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Applications of ceftiofur hydrochloride in prevention of mycobacterium tuberculosis infection

A technology of ceftiofur hydrochloride and Mycobacterium tuberculosis, which is applied in the field of pharmacy and can solve the problems of no report on the application of ceftiofur hydrochloride and the like

Inactive Publication Date: 2018-06-08
TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, the application of ceftiofur hydrochloride in an

Method used

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  • Applications of ceftiofur hydrochloride in prevention of mycobacterium tuberculosis infection
  • Applications of ceftiofur hydrochloride in prevention of mycobacterium tuberculosis infection
  • Applications of ceftiofur hydrochloride in prevention of mycobacterium tuberculosis infection

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Embodiment Construction

[0014] In order to better illustrate the present invention, specific embodiments of the present invention will be described in detail below.

[0015] 1. Expression and purification of methionyl aminopeptidase (MetAP) in Mycobacterium tuberculosis

[0016] According to the literature (Lu J P, Ye Q Z. Expression and characterization of Mycobacterium tuberculosis methionine aminopeptidase type 1a.[J].Bioorganic&Medicinal Chemistry Letters,2010,20(9):2776-2779.)

[0017] (1) Transform the pET28a vector containing the MetAP gene into the Escherichia coli BL21 (DE3) strain, and use LB plate medium (containing 50 mg / L kanamycin) to screen for positive clones.

[0018] (2) Pick positive clones on the plate and transfer them to 0.8L LB medium (containing 50mg / L kanamycin) after cultivating overnight at 37°C. At 0.6, add 0.1 mM IPTG (Isopropylβ-D-Thiogalactoside) and incubate at 20° C. for 18 hours.

[0019] (3) Collect the cells by centrifugation at 5000rpm for 10min and then autocla...

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Abstract

The invention provides a compound especially used for inhibiting methionine aminopeptidase (MetAP) in mycobacterium tuberculosis (Mtb). The compound is ceftiofur hydrochloride, and possesses obvious inhibition activity on methionine aminopeptidase in mycobacterium tuberculosis. The compound can be used for preparing small molecular inhibitors used for inhibiting methionine aminopeptidase (MetAP) in mycobacterium tuberculosis (Mtb), and is promising to be prepared into drugs used for preventing Mtb infection.

Description

technical field [0001] The invention relates to the technical field of pharmacy, in particular to the application of ceftiofur hydrochloride in anti-mycobacterium tuberculosis infection. Background technique [0002] Mycobacterium tuberculosis (Mtb) is the pathogenic bacterium that causes tuberculosis (TB). It is mainly transmitted through the respiratory tract and can cause symptoms such as cough, chest pain, hemoptysis, and dyspnea. Healthy people infected with tuberculosis do not necessarily have the disease, only when the body's immunity is weakened. Statistics from the World Health Organization (WHO) show that 8 to 10 million cases of tuberculosis occur in the world every year, and about 3 million people die from tuberculosis every year. It is the single infectious disease that causes the largest number of deaths. In 1993, WHO declared a "global tuberculosis emergency", arguing that tuberculosis has become an important public health problem all over the world. [0003...

Claims

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Application Information

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IPC IPC(8): A61K31/546A61P31/04C07D501/36
CPCA61K31/546C07D501/36
Inventor 杨海涛李瑞张晓玲刘祥胡灿张晴晴
Owner TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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