Fluorescence quantitative PCR primer, probe and kit for detecting ordinary pathogenic fungi

Abstract

The invention discloses a fluorescence quantitative PCR primer, a probe and a kit for detecting ordinary pathogenic fungi. A specific primer and a TaqMan probe are independently designed, and a fluorescence PCR detection method which can be used for simultaneously detecting 15 clinically ordinary pathogenic fungi, including 8 candida mycoderma bacteria (candida albicans, candida glabrata, candida parapsilosis, candida kefyr, candida sake, candida kruse, candida guilliermind and candida tropicalis), four aspergilli (aspergillus niger, aspergillus flavus, aspergillus terreus and aspergillus fumigates), cryptococcus, rhizopus oryzae and mucor circinelloides, is established. The method is relatively high in sensitivity and specificity, and has significant meanings for early diagnosis and treatment on invasive infections with fungi.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and relates to a real-time fluorescent PCR detection kit, in particular to a kit and a detection method for quickly identifying common pathogenic fungi in clinical samples using real-time fluorescent quantitative PCR technology. Background technique [0002] In recent years, with the widespread use of antibiotics, immunosuppressants and corticosteroids in clinical practice, organ transplantation, catheter intubation, etc. have been widely carried out, and the rate of deep fungal infection has increased. Deep fungal infection is a severe infection with a mortality rate of 40-90%. The incidence of fungal diseases has been on the rise in recent years, especially for intensive care units, immunodeficiency patients, and cancer patients. 8% of hospital infections are caused by fungi . [0003] According to statistics, the incidence of deep fungal infections has increased by 3-5 times in the past 30 years, and fu...

AI Technical Summary

Problems solved by technology

There are certain defects in the above methods: the traditional culture method is the most direct method for diagnosing fungal infection with high accuracy, but its shortcomings of long time consumption, easy pollution and low sensitivity make it unfavorable for clinical diagnosis of patients; The high-resolution machine directly observes the patient's lesion dynamically, which has important prompting value, but this method not only has low specificity, but also has early diagnostic value only for high-risk patients; serological methods have certain sensitivity and specificity, but the diagnosis higher false positive rate

[0006] Common clinical invasive fungi mainly include Candida, Aspergillus, and Cryptococcus, etc., and no reports have been found on the detection of different fungal genera by fluorescent PCR using the probe method

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