Marine Streptomyces strain and use thereof

A marine Streptomyces and Staphylococcus technology, applied in bacteria, antifungal agents, antibacterial drugs, etc., can solve problems such as increased drug resistance and reduced efficacy of antibiotics

Inactive Publication Date: 2011-10-19
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The abuse of clinical antibiotics has sharply increased the drug resistance of pathogenic bacte...

Method used

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  • Marine Streptomyces strain and use thereof
  • Marine Streptomyces strain and use thereof
  • Marine Streptomyces strain and use thereof

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Experimental program
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Embodiment 1

[0025] Embodiment 1, the isolation of marine streptomyces FXJ7.388

[0026] 1. Separation of strains

[0027] In January 2009, sea mud samples from the Bohai Sea were obtained from Dalian Bay, and the samples were brought back to the laboratory for processing as soon as possible. After the sea mud sample is naturally air-dried, it is made into a suspension with sterile water, pretreated by heating at 60°C for ten minutes, using ISP-3 medium as the isolation medium, and cultured at 28°C for 30 days, and single colonies are picked in GYM Purified on the culture medium to obtain marine Streptomyces FXJ7.388.

[0028] 2. Identification of strains

[0029] 1. 16S rRNA analysis

[0030] Genomic DNA of Streptomyces marine FXJ7.388 was extracted, and the 16S rRNA gene was amplified and sequenced using actinomycete 16S rRNA universal primers (27f: 5'-AGAGTTTGATCMTGGCTCAG-3'; 1492r: 5'-TACGGYTACCTTACGACTT-3') and purified for sequencing. Similarity comparisons were performed by NCBI...

Embodiment 2

[0055] Embodiment 2, the antibacterial function verification of marine Streptomyces FXJ7.388

[0056] 1. Antibacterial experiment of marine Streptomyces FXJ7.388 by agar block method

[0057] 1. The marine Streptomyces FXJ7.388, Streptomyces xinghaiensis NRRL B-24674 T and Streptomyces chungwhensis DSM 41843 were inoculated in GYM agar medium (glucose 4.0g, yeast extract 4.0g, malt extract 10.0g, calcium carbonate 2.0g, agar 15.0g, distilled water 1000.0ml), and cultured at 28°C for 2 weeks.

[0058] 2. Use LB liquid medium for the test bacteria, shake culture at 37°C for 24 hours; use PDA liquid medium for fungi, shake culture at 25°C for 48 hours. The rotating speed of the oscillator is 165r / min, and the obtained concentration is 10 8 Cfu / ml of the bacteria solution for the test bacteria, after mixing the medium and the bacteria solution, pour it into the petri dish for activity determination, and cool it until the medium solidifies.

[0059] Tested bacteria: Staphylococc...

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Abstract

The invention provides a marine Streptomyces strain and use thereof. The strain provided by the invention is Streptomyces sp. FXJ7.388, and the collection number of the strain is CGMCC No.4474. The invention also provides the use of the strain in the preparation of an antibacterial product. The antibacterial effect is to inhibit gram-positive bacteria, including Multi-resistant Staphylococcus aureus (MRSA 1-1), staphylococcus aureus CGMCC1.2386, bacillus subtilisGCMCC1.2428 and/or Candida albicans 2.538. Experiments prove that the Streptomyces sp. FXJ7.388 separated by the invention has the advantage of resisting gram-positive bacteria and Candida albicans, namely having high activity for resisting MRSA, staphylococcus aureus AND bacillus subtilis and certain inhibiting effect on Candida albicans.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of marine streptomyces and its application. Background technique [0002] Actinomycetes are a class of Gram-positive bacteria with high G+C mol% content of genomic DNA and complex morphological differentiation. They are named because their colonies are mostly radial. Actinomycetes can produce a variety of biologically active substances with high economic value, including antibiotics, and are considered to be an important class of medicinal microbial resources. Among the more than 20,000 microbial-derived biologically active substances discovered so far, about Half are produced by actinomycetes. [0003] The abuse of clinical antibiotics has sharply enhanced the drug resistance of pathogenic bacteria, and the efficacy of existing antibiotics has been greatly reduced. It is urgent to find new antibiotics. As the odds of discovering new active products in soil-derived actinom...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P31/04A61P31/10C12R1/465
Inventor 黄英刘宁郗丽君张利敏
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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