36 results about "Microtitre plate" patented technology

A robotic arm-mounted laser barcode reader is provided. The robotic arm-mounted laser barcode reader may be used in the field of sample analysis to in increase the rate with which sample carriers such as microtitre plates may be interfaced with an automated system. The robotic arm-mounted laser barcode reader can read a bar code label affixed to a sample carrier immediately when the robotic arm grabs and manipulates a sample carrier, eliminating the step of presenting the sample carrier to a separate bar code reader to ensure proper tracking and/or inventorying of the sample carrier. The robotic arm-mounted laser barcode reader may also be used in other automated apparatuses that require tracking and/or inventorying.

By arranging reagents needed for a specific reaction in separate distinct chambers in a reagent cartridge and combining several such reagent cartridges in a cassette in a format that matches that of commonly used microtitre plates. e.g. the 96 well format or other standard formats. several drawbacks of well-known procedures can be eliminated.

The invention concerns an optical instrument for imaging fluorescence signals from an arrangement of a plurality of individual detection sites, for example the wells of a microtitre plate. In order to improve the light yield of the fluorescence excitation with excitation light as well as the light yield of the detection of the fluorescence signals, an objective array is provided which is arranged in the beam path between the field lens and the detection sites and comprises a field lens array with field lens array elements and a pupil lens array with pupil lens array elements. In order to improve the channel separation and suppress interfering light the objective array can comprise a diaphragm array with in each case two diaphragm openings per detection site.

A method and device to assist in the determination of protein domain boundaries is described. In particular the device is designed to provide a high throughput of proteolytic digestion of proteins to identify domains and their boundaries, for use in protein structure determination, in a manner that is amenable to automation. Proteases are immobilized in a convenient format such as a microtitre plate and preferably arranged in a matrix thereby allowing for simultaneous degradation of a protein by a number of proteases at a number of concentrations.

A shuttle apparatus for transferring sample carriers comprising a shuttle table having a mating section, the mating section allowing a sample carrier to be transferred between the shuttle table and a mating support structure. The shuttle table can optionally further comprise a mating section having a void section.

Disclosed herein is a method of: treating an organic polymer with an electron beam-generated plasma; exposing the treated polymer to air or an oxygen- and hydrogen-containing gas, generating hydroxyl groups on the surface of the polymer; reacting the surface with an organosilane compound having a chloro, fluoro, or alkoxy group and a functional or reactive group that is less reactive with the surface than the chloro, fluoro, or alkoxy group; and covalently immobilizing a biomolecule to the functional or reactive group or a reaction product thereof.

This invention provides a high throughput survival assay, using uptake of a marker dye (e.g. a fluorescent dye) as a marker of death of a nematode. The assay permits high throughput screening of thousands of compounds possible. By the application of automated worm handling technology we are able to accurately dispense nematodes into 384 well microtitre plates, at rates many thousand of Limes faster than previously possible. In addition, we have automated the analysis of survival by the use of a fluorometric plate reader that quantitates the degree of fluorescence within each well.

A fluorescence microtitre plate reader comprises a plate reader body (2) having a base (3), top (5) and sidewalls (4), the top of the plate reader comprising at least one seat (6) dimensioned for receipt of a microtitre plate (7), the at least one seat defining an aperture (8) in the top of the plate that substantially corresponds to a base of the plate. The plate reader also includes means for illuminating wells of a microtitre plate with light from beneath the plate having an excitation wavelength, and a camera (10) and lens (11) assembly for capturing light emitted by the wells of the microtitre plate and generating a digital image, in which regions of light intensity of the image correspond to wells of the microtitre plate. An optical system is disposed within the plate reader body for expanding the distance to image plane of light, the optical system comprising a first mirror (15) disposed to receive light from the wells of the microtitre plate and reflect the light to second mirror (16) which is disposed to reflect incident light to the camera lens. The means for illumining the wells of the microtitre plate comprises a ring light (12) that embraces the camera and lens assembly.

Some of the blind holes (6) between indentations (4) of a heating surface (3) contain lifting elements (7) which, after opening of a cover, release a microtitre plate (13) from the heating surface (3) and raise said microtitre plate about 2 to 3 mm, so that it can be removed without application of force. Each lifting element (7) consists of a coil spring (8) and a contact pin (9) made of, for example, PEEK which is inserted into said coil spring and presses with a round flat abutting surface (12) against the lower surface of the microtitre plate (13). The spring constant of the lifting element (7) is about 6 N/mm.

Method for the detection of a pathogenic form of a prion protein in a sample, comprising providing a container, pretreating the container to deposit on a surface of the container a coating of a cellulose derivative capable of favoring the binding of pathogenic prion protein to said container surface over the binding of cellular prion protein, incubating the sample in said container to bind any pathogenic prion protein present in the sample to said container surface, labelling the thus immobilized pathogenic prion protein, if present, with an appropriate labelling agent using an anti-prion antibody capable of binding to pathogenic prion protein and detecting the presence of labelling agent attached to the container surface. Coating a surface of a microtitre plate well with nitrocellulose allows to perform an ELISA for quantification of pathogenic prions in a suspected sample, especially after enzymatic digestion of the sample with an enzyme like proteinase K, by enhancing binding of pathogenic prions and/or reducing binding of cellular (non-pathogenic) prions.

High-affinity monoclonal antibodies, wherein the affinity is characterised by: (i) incubating first and second samples of the antibody in antigen-coated microtitre plate wells at a concentration chosen to be within the linear part of a standard curve at pH 7.2 for 1 hour at 37 DEG C; (ii) removing unbound antibody from both samples; (iii) incubating the first sample with PBS at pH 7.2 for 1 hour at 37 DEG C, and reducing the pH of the second sample to pH 3 or below and incubating for 1 hour at 37 DEG C; (iv) removing unbound antibody from both samples; (v) incubating both samples with anti-antibody alkaline phosphatase-conjugate for 1 hour at 37 DEG C; (vi) removing unbound conjugate from both samples; and (vii) adding PNPP substrate to the samples, measuring the absorbance of the samples at 405 nm, and determining the amount of antibody bound to antigen, wherein the amount bound in the second sample is > 50 % of that of the first sample.

Various embodiments include a sample storage system having: a transport module; a storage module coupled with the transport module on at least one side of the transport module, the storage module for storing a plurality of specimen tubes or microtitre plates; a tube selector module coupled to an end of the transport module for selecting at least one of the plurality of specimen tubes or microtitre plates; and an input/output (I/O) module coupled with the transport module on a side of the transport module, wherein the transport module is configured to modularly couple with at least one additional storage module for storing a plurality of specimen tubes or microtitre plates.

The invention discloses a method for detecting bacterial drug resistance in aquaculture, which comprises the following steps: (1) an antibacterial drug stock solution is prepared; (2) a bacterial liquid is prepared; (3) an antibiotic working solution is prepared from the antibacterial drug stock solution obtained in step (1); (4) a 96-well microtitration plate is placed in an incubator for incubation; and (5) data are read through a microplate reader for analysis. The method applies to bacterial drug resistance detections in aquaculture and other areas. In combination of the specification of the 96-well microtitration plate (8 multiply 12) and the drug concentration scope required by CLSI (Clinical and Laboratory Standards Institute), 8 categories of antibiotic drugs and 10 concentration gradients are selected for detection. When the method is in use, no flat plate is required, the steps of configuring a large amount of culture mediums, manufacturing drug sensitive paper scraps and measuring the inhibition zone are spared, and a small quantity of culture solutions is required. The method has the advantages of strong practicability, convenience, accuracy and stability in results, good repeatability and high sensitivity.

The present invention relates to methods and systems for adding a reagent to an analyte in a gel. The invention further provides methods and systems for transferring liquid analyte reagent mixtures from a gel to a second vessel, such as a microtitre plate. The invention is useful in the manipulation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for manipulating proteins and peptides in isoelectric focusing gels.

A microtitre plate having a relieved perimeter includes a plate defining a plurality of wells and the perimeter of the plate is horizontally relieved. Alternatively, a microtitre plate may include a base and a holding section extending from the base. The holding section defines a plurality of wells and the perimeter thereof being horizontally relieved.