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Method for the detection of a pathogenic form of a prion protein

A prion protein, prion technology, used in biochemical equipment and methods, disease diagnosis, biological testing, etc.

Inactive Publication Date: 2006-08-16
桑昆血液供给基金会
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzyme treatment without PK, another problem is PrP C and PrP Sc Binds to microtiter plate with equal intensity

Method used

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  • Method for the detection of a pathogenic form of a prion protein
  • Method for the detection of a pathogenic form of a prion protein
  • Method for the detection of a pathogenic form of a prion protein

Examples

Experimental program
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Embodiment

[0054] In order to demonstrate the effect of a clear nitrocellulose coating on the prion-binding properties of microtiter plates, applicants performed experiments with nitrocellulose-coated microtiter plates (NC-plates) or standard microtiter plates (Std-plates). Direct ELISA. Homogenates (0.1%) of scrapie-infected hamster brains (263K strain) or normal mouse brains were treated with or without PK; and performed on NC-plates (0.1% nitrocellulose-coated) or Std-plates Incubation. Bound prion protein was detected with horseradish peroxidase (HRP)-labeled anti-prion antibody 1E4, followed by staining with 3,3',5,5'-tetramethylbenzidine (TMB) substrate.

[0055] as in figure 1 Prion protein (PrP) present in the brain of scrapie-infected hamsters digested by PK Sc ) binds to the NC-plate, but not to the Std-plate. In addition, prion protein (PrP) present in undigested normal mouse brain C ) binds weaker to the NC-plate than to the Std-plate.

[0056] In order to demonstrate t...

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Abstract

Method for the detection of a pathogenic form of a prion protein in a sample, comprising providing a container, pretreating the container to deposit on a surface of the container a coating of a cellulose derivative capable of favoring the binding of pathogenic prion protein to said container surface over the binding of cellular prion protein, incubating the sample in said container to bind any pathogenic prion protein present in the sample to said container surface, labelling the thus immobilized pathogenic prion protein, if present, with an appropriate labelling agent using an anti-prion antibody capable of binding to pathogenic prion protein and detecting the presence of labelling agent attached to the container surface. Coating a surface of a microtitre plate well with nitrocellulose allows to perform an ELISA for quantification of pathogenic prions in a suspected sample, especially after enzymatic digestion of the sample with an enzyme like proteinase K, by enhancing binding of pathogenic prions and / or reducing binding of cellular (non-pathogenic) prions.

Description

Technical field: [0001] The present invention relates to the field of prion diseases. These diseases, also known as transmissible spongiform encephalopathy (TSE), include bovine spongiform encephalopathy (BSE) or mad cow disease in cattle, scrapie in sheep and Creutzfeldt-Jakob disease (CJD) in humans. [0002] More particularly, the invention relates to the field of diagnostic methods and the diagnosis of prion diseases or TSE. Typically, such methods expect to detect (pathogenic) conformers (conformers) of native (non-pathogenic) prion proteins, especially in animal or human brains, in lymph fluid; or where possible Such conformers are found in body fluids such as blood, plasma, serum or urine. Background technique: [0003] The present invention relates to methods of diagnosing prion diseases or TSE in tissue samples and other biological samples such as bodily fluids. [0004] Prion diseases or TSEs are a group of lethal, non-inflammatory neurodegenerative disorders su...

Claims

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Application Information

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IPC IPC(8): G01N33/68C12Q1/70
CPCG01N2800/2828G01N33/6896
Inventor 约瑟夫斯·威廉默斯·阿方萨斯·马里亚·范奥尔斯特温·扬·卡雷尔·范德福斯特科内利斯·埃里克·哈克弗朗西斯库斯·安东尼厄斯·科内利斯·范恩格伦伯格
Owner 桑昆血液供给基金会
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