Technology for extracting proteins and superoxide dismutase (SOD) from horseradish tree leaves

Abstract

The invention discloses a technology for extracting proteins and superoxide dismutase (SOD) from horseradish tree leaves. The technology comprises the following steps: smashing and pulping fresh horseradish tree leaves, and performing digestion, pressure filtering, isoelectric precipitation and centrifugation to obtain precipitates and supernate; performing modification protection, thermal denaturation reaction and freeze drying on the precipitates to obtain SOD freeze-dry powder; performing isoelectric precipitation, centrifugation and spray drying on the supernage to obtain leaf protein powder. By the adoption of the technical methods such as normal-temperature digestion, isoelectric precipitation, centrifugal separation, the original functional characteristics of the proteins and the activity of enzyme are retained to the maximum extent, and the yield of the proteins and that of active enzyme are greatly increased; the obtained leaf protein powder is bright in color, high in protein content and stable in quality; the obtained SOD is high in activity, and the practical value and the economical benefit are higher. The technology is easy to operate, low in production cost, safe, reliable and suitable for industrial production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a process for extracting protein and superoxide dismutase (SOD) from Moringa oleifera leaves. Background technique [0002] Moringa is a perennial woody plant native to the Himalayas in northern India. It is a tropical plant with high edible and medicinal value. A large number of studies have proved that Moringa leaves are rich in protein, total flavonoids and superoxide dismutase (SOD) and other functional components. The protein content of Moringa leaves is as high as 27%, about twice that of milk; the superoxide dismutase (SOD) contained in Moringa leaves has the functions of scavenging free radicals, regulating thyroxine and hepatic lipid peroxidation. In recent years, Moringa has been widely introduced and cultivated in my country's Guangdong, Yunnan and Hainan, and the development of its related products has become a hot spot. But the domestic research on Moringa mainly focus...

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Problems solved by technology

However, domestic research on Moringa mainly focuses on cultivation techniques and nutrient analysis, and there are no patent reports about extracting protein and superoxide dismutase (SOD) technology from Moringa leaves.

[0003] In the existing extraction process of other plant leaf proteins, heat treatment is generally used to extract and separate leaf proteins. This method not only consumes a lot of energy, but also easily causes the denaturation of the extracted leaf proteins, and the rehydration solubility is poor, and the digestibility is not good. High, low commercial application value

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