Detection kit for S100B protein and using method of detection kit

Abstract

The invention is applicable to the field of biotechnology, and particularly relates to a detection kit for S100B protein and a using method of the detection kit. The kit comprises an elisa plate coated with an anti-S100B monoclonal antibody, a horse radish peroxidase labeled anti-S100 polyclonal antibody solution for detection, a standard substance, a standard substance diluent, a sample diluent,a concentrated washing solution, a developing solution A, a developing solution B, a stop solution and a detection antibody diluent. The kit disclosed by the invention is relatively high in sensitivity, and the lowest detection limit of the kit is as low as 1pg / mL; the uniformity is good, the stability is high, the intra-batch variation coefficient is less than 10%, and the inter-batch variation coefficient is less than 5%; the specificity is good, the cross reaction between the kit and serum total bilirubin TBIL, triglyceride TG and total cholesterol TC which are easy to interfere with the test in serum is very low, and the measurement of S100B is not interfered; the clinical trial effect is good, and the single detection cost is lower than that of the same type of kit on the market.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a detection kit for S100B protein and a use method thereof. Background technique [0002] S100B protein is a specific protein that exists in glial cells in the brain. It has a wide range of biological activities and plays a variety of important physiological functions at physiological concentrations, including affecting the growth, reproduction and differentiation of glial cells, maintaining Calcium homeostasis, and may play a certain role in learning and memory. When the glial cell membrane is damaged due to brain injury, S100B protein is released into the extracellular space and enters the cerebrospinal fluid and blood through the damaged blood-brain barrier. Its serum content is closely related to the condition and prognosis of some nervous system diseases. [0003] At present, the diagnosis of craniocerebral injury and cerebrovascular diseases mainly relies on imagin...

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Problems solved by technology

[0008] The embodiment of the present invention provides a detection kit for S100B protein, which aims to solve the problems of high detection cost, low detection sensitivity, poor specificity and stability in existing detection methods for serum S100B

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