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39 results about "Nibea albiflora" patented technology

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Molecular marker for identifying genetic gender of nibea albiflora and application thereof

ActiveCN107326077AImprove aquaculture profitabilityRapid identificationMicrobiological testing/measurementDNA/RNA fragmentationEconomic benefitsNucleotide sequencing

The invention discloses a molecular marker for identifying the genetic gender of nibea albiflora and application thereof. The molecular marker shows insertion/deletion length polymorphism of a nucleotide sequence shown as SEQ ID NO: 1; an individual having the nucleotide sequence shown as SEQ ID NO: 1 is a female nibea albiflora, and an individual without the nucleotide sequence shown as SEQ ID NO: 1 is a male nibea albiflora. The invention further discloses a primer pair, a kit and a method for detecting the molecular marker. The molecular marker can simply, conveniently, rapidly and stably identify the genetic genders of different individuals in each colony of the nibea albiflora, so that development of a unisexual breeding technology of the nibea albiflora and development of unisexual breeding of the nibea albiflora are facilitated, the breeding efficiency is further improved and nibea albiflora breeding economic benefits are increased; meanwhile, nibea albiflora gender determining mechanism and other relevant scientific researches are benefited.

Molecular marker for identifying genetic gender of nibea albiflora and application thereof

Molecular marker for identifying genetic gender of nibea albiflora and application thereof

Molecular marker for identifying genetic gender of nibea albiflora and application thereof

Owner:JIMEI UNIV

Basic bait formulation for Nibea albiflora

InactiveCN103070301AGreat tasteLow costAnimal feeding stuffVitamin CAdditive ingredient

The invention relates to a basic bait formulation for Nibea albiflora. The basic bait formulation for the Nibea albiflora is characterized by comprising fish meal, peanut meal, soybean phospholipid, fish oil, soybean oil, corn starch, vitamin C phosphate, choline, tea polyphenol, composite vitamin, composite amino acid, a composite mineral substance and a bonding agent. Compared with the prior art, the fish meal and the peanut meal are taken as main raw materials and various beneficial components are added, so that the problems that amino acids are unbalanced as well as lysine, methionine and threonine are short in the peanut meal can be effectively solved; the amino acids are balanced by adding synthetic amino acids or other protein feeds, so that all nutritional requirements can be supplied to the Nibea albiflora; multiple effective antibacterial substances including the tea polyphenol are added into the feed to prevent the feed from going mouldy and deteriorating, so that artificial breeding is facilitated; the bait is good in feed attractant effect, has a higher food coefficient than natural bait and is relatively applicable to livestock and aquatic feeds; and the peanut meal is low in cost, so that compared with the feed in the prior art, the bait provided by the invention saves cost and is high in economic benefit.

Basic bait formulation for Nibea albiflora

Basic bait formulation for Nibea albiflora

Basic bait formulation for Nibea albiflora

Owner:ZHEJIANG OCEAN UNIV

Nibea albiflora feed and preparing method of nibea albiflora feed

ActiveCN104000009AImprove palatabilityGreat tasteFood processingClimate change adaptationBiotechnologyAdditive ingredient

The invention discloses nibea albiflora feed and a preparing method of the nibea albiflora. The nibea albiflora feed is soft particle feed prepared from fresh trash fish and nutrition ingredients through being matched, wherein the nutrition ingredients comprise soybean meal, corn gluten meal, high gluten flour, complex vitamin, complex minerals, choline chloride and complex enzyme preparations. The fresh trash fish and the nutrition ingredients are adopted and are mixed to be prepared into the soft particle feed, nutrition components of the feed are supplemented, and the mouth feeling of the feed is also improved, so the feed is favorable for the ingestion and the growth of nibea albiflora. Meanwhile, various kinds of vitamin, minerals and complex enzyme required by the nibea albiflora are added, and phototrophic bacteria are added, so the nutrition value of the feed is improved, and the survival rate of the nibea albiflora is improved.

Nibea albiflora feed and preparing method of nibea albiflora feed

Nibea albiflora feed and preparing method of nibea albiflora feed

Nibea albiflora feed and preparing method of nibea albiflora feed

Owner:ZHOUSHAN HAIYOO SCI & TECH

Nibea albiflora juvenile fish compound feed with fish meal replaced by fermented soybean meal and feeding method

InactiveCN105594998AReduce anti-nutritional factorsImprove balanceFood processingClimate change adaptationCelluloseJuvenile fish

The invention provides Nibea albiflora juvenile fish compound feed with fish meal replaced by fermented soybean meal. The compound feed is prepared from, by weight, 14-70 parts of fish meal, 10-67 parts of fermented soybean meal, 1-6 parts of fish oil, 1-6 parts of soybean oil, 5-13 parts of dextrin, 0.5-2.5 parts of soybean lecithin, 0.2-1.6 parts of seawater fish compound premix feed, 0.1-0.6 part of choline chloride, 0.5-2.0 parts of monocalcium phosphate, 0.05-0.2 part of hydroxyl methionine, 0.02-0.08 part of ethyoxyl quinoline and 0.3-15 parts of cellulose. Compared with the prior art, on the premise of not affecting healthy growth of juvenile fish, the use amount of the fish meal can be reduced to about 43% or even about 25%, and feed cost is greatly reduced. The protein source amino acid is balanced, the compound feed is high in palatability, and beneficial for fish feed ingestion and feed digestion and absorption, and it is proved through experiments that the feed can ensure healthy growth of the juvenile fish.

Owner:MARINE FISHERIES RES INST OF ZHEJIANG

Method for developing SSR (Simple Sequence Repeat) molecular mark of nibea albiflora for population identification

InactiveCN105624322AImprove work efficiencyReduce screening costsMicrobiological testing/measurementDNA/RNA fragmentationGenomic DNAGrowing season

The invention discloses a method for developing an SSR (Simple Sequence Repeat) molecular mark of nibea albiflora for population identification. The method comprises the following steps: extracting RNA (Ribonucleic Acid) by using a nibea albiflora mixed tissue of a plurality of nibea albiflora, performing RNA sequencing, performing splicing to obtain a nibea albiflora expression gene sequence, and obtaining a potential SSR mark with a bioinformatics method; designing primers by utilizing an SSR flanking sequence, extracting genomic DNA (Deoxyribose Nucleic Acid) by using fins from a random sample, and screening the SSR primers; and performing PCR (Polymerase Chain Reaction) amplification on different nibea albiflora DNA templates by using each pair of the SSR primers, performing capillary electrophoresis detection on an amplification product by using a Qsep100 full-automatic nucleic acid analysis system, screening out primers with different amplification results, calculating allelic composition and allelic frequency by utilizing PopGene, and analyzing a nibea albiflora population relationship and a genetic structure. The SSR molecular mark method for identifying and verifying nibea albiflora groups, provided by the invention, is stable and reliable, is not influenced by ages, gender and growth seasons, and provides scientific bases for group analysis as well as species identification, breeding and evaluation of the nibea albiflora.

Method for developing SSR (Simple Sequence Repeat) molecular mark of nibea albiflora for population identification

Method for developing SSR (Simple Sequence Repeat) molecular mark of nibea albiflora for population identification

Method for developing SSR (Simple Sequence Repeat) molecular mark of nibea albiflora for population identification

Owner:JIMEI UNIV

Nibea albiflora fry artificial mixed feed domestication method

ActiveCN104054597AImprove seedling survival rateThere was no mass deathClimate change adaptationPisciculture and aquariaFisheryObserved Survival

The invention discloses a nibea albiflora fry artificial mixed feed domestication method. The method adopts the following steps: 1, prolonging feeding tine of rotifer and artemia nauplius; 2, applying artificial mixed feed domestication in advance; 3, performing lamplight induction aggregation domestication; 4, separating floating fry to culture. Therefore, the artificial mixed feed domestication of the nibea albiflora fry can be finished, and the survival rate of the nibea albiflora fry can be improved. Through the adoption of the method to conduct artificial mixed feed domestication on the nibea albiflora fry, 80% 35-day nibea albiflora fry can successfully convert to artificial mixed feed, 20% 35-day nibea albiflora fry floats, 60% 35-day nibea albiflora fry can successfully convert to fry which can eat artificial mixed feed after follow-up domestication, the batch death phenomenon does not occur, and the survival rate of fry breeding can reach 35%.

Owner:MARINE FISHERIES RES INST OF ZHEJIANG

Culturing method of nibea albiflora

InactiveCN102499126AInhibition of reproductionAvoid spreadingClimate change adaptationPisciculture and aquariaDiseaseEutrophication

The invention provides a culturing method of nibea albiflora. The method comprises the following steps: treating frozen nibea albiflora in bait with formalin (20-50 mg/L) dipping bath for 4 to 6 minutes; controlling the feeding amount according to the weight of the nibea albiflora and controlling the culture density according to the weight of the nibea albiflora when mixed feed is used; performing regular medicated bath with seawater solution of mixture with the mass ratio of copper sulfate to ferrous sulfate of 7 to 3; reinforcing clean equipment, removing sludge at the bottom of a pool, and changing water to play the roles of killing amyloodinium ocellatum spores, removing pool water eutrophication generation conditions and eliminating a possibly existing amyloodinium ocellatum spreading source when the temperature of aquatic water exceeds 22 DEG C. Therefore, spreading of amyloodinium ocellatum is cut off, and propagation of the amyloodinium ocellatum is inhibited. Compared with the prior art, regarding the characteristics in nibea albiflora culture, the invention provides the culturing method of nibea albiflora capable of preventing occurrence and spreading of amyloodinium ocellatum disease, and the occurrence rate of the amyloodinium ocellatum disease is reduced to 20 percent of the previous occurrence rate after the method is used.

Owner:MARINE FISHERIES RES INST OF ZHEJIANG

Method for producing sterile hybrid nibea albiflora

ActiveCN106614142AImprove survival rateStrong stress resistanceClimate change adaptationPisciculture and aquariaJuvenile fishPennahia argentatus

The invention discloses a method for producing sterile hybrid nibea albiflora. The method includes the following steps: using nibea mitsukurii as a female parent and pennahia argentatus as a male parent, and promoting the sexual glands of the nibea mitsukurii and the pennahia argentatus to be mature synchronously; selecting the nibea mitsukurii with well-developed sexual glands and the pennahia argentatus with well-developed sexual glands, and injecting lutein release promoting hormones for artificially induced spawning of the nibea mitsukurii; collecting an ovum of the nibea mitsukurii and a sperm of the pennahia argentatus, and conducting dry fertilization; breeding hybrid larva fish, hybrid young fish and hybrid juvenile fish respectively, and observing the sexual gland. The hybridization method is used for culturing the nibea albiflora with the sterile sexual gland, and the bred nibea albiflora has the advantages of being high in survival rate, strong in stress resistance, totally sterile and the like. The method has the advantages of being simple and easy to operate, and the sterile hybrid nibea albiflora has a good application prospect in aquaculture production, can be used as an artificial 'surrogate' receptor, and has very important application value.

Method for producing sterile hybrid nibea albiflora

Method for producing sterile hybrid nibea albiflora

Method for producing sterile hybrid nibea albiflora

Owner:MARINE FISHERIES RES INST OF ZHEJIANG

Fish wintering aquaculture net cage and method for wintering aquacultured Nibea albiflora by utilizing net cage

ActiveCN103814850AImprove the blocking effectImprove concentrationClimate change adaptationPisciculture and aquariaEngineeringSurface structure

The invention relates to a fish wintering aquaculture net cage and a method for wintering aquacultured Nibea albiflora by utilizing the net cage, and belongs to the technical field of fish aquaculture. The fish wintering aquaculture net cage comprises a canvas hood arranged all around and a rigid support provided with a canvas layer at the bottom, wherein the canvas hood is formed by connecting four sheets of canvas matched with four surfaces of the rigid support through four zippers; the area of the canvas layer is greater than that of the bottom of the rigid support; the canvas layer is of a convex cambered surface structure; the center of a convex cambered surface is connected with a discharge pipe. According to the wintering aquaculture net cage disclosed by the invention, the canvas hood can be opened and closed, when the canvas hood is closed, a relatively stable and still closed aquaculture space is formed in the net cage so as to achieve a better heat preservation effect for wintering the aquacultured Nibea albiflora, when peripheral water is polluted, a better blocking effect can also be achieved, and when the canvas hood is opened, the net cage can also be used for aquaculture in regular seasons; the fish wintering aquaculture net cage and the method are a product and an aquaculture method worthy of large-area promotion.

Fish wintering aquaculture net cage and method for wintering aquacultured Nibea albiflora by utilizing net cage

Fish wintering aquaculture net cage and method for wintering aquacultured Nibea albiflora by utilizing net cage

Fish wintering aquaculture net cage and method for wintering aquacultured Nibea albiflora by utilizing net cage

Owner:MARINE FISHERIES RES INST OF ZHEJIANG

Functional feed used for inhibiting growth of gonads of Nibea albifloras and preparation method thereof

InactiveCN105410466ALow costPromote weight gainFood processingClimate change adaptationCastor SeedSodium Bentonite

The invention discloses functional feed used for inhibiting growth of gonads of Nibea albifloras and a preparation method thereof. The fish feed comprises the following raw materials: super steamed fish meal, wheat flour, cottonseed meal, fish oil, corn protein powder, distiller's grain protein feed, bean pulp, beer yeast, vital gluten, phospholipid oil, squid ointment, spray-dried blood cells, monocalcium phosphate, bentonite, castor seed kernels, special fish microelements, choline, mildew preventive, special fish multivitamin, vitamin, antioxidant, inositol and carnitine, wherein every 1000 kg of raw materials contain 8.3 kg-9.85 kg of bentonite and 6.5 kg-8 kg of castor seed kernels. The fish feed is low in cost, and can effectively inhibit the growth of the gonads and the natural ovulation of the Nibea albifloras in the growth period of the gonads of the Nibea albifloras to promote weight growth and improves economic benefit of culturing. The preparation method of the fish feed is simple and convenient in operation and is beneficial for improving culturing efficiency.

Functional feed used for inhibiting growth of gonads of Nibea albifloras and preparation method thereof

Owner:SHANGHAI NONGHAO FEED

Inducing method of Nibea albiflora gynogenesis diploid

InactiveCN103430872AImprove and accelerate homozygous efficiencyOvercoming the Effects of Haploid SyndromeClimate change adaptationPisciculture and aquariaSemenMatus ovatus

The invention discloses an inducing method of a Nibea albiflora gynogenesis diploid. The technical scheme of the invention is that the inducing method comprises the steps of genetic inactivation of sperms of Nibea albiflora and diploidzation of ova of the Nibea albiflora; the inducing method is mainly characterized by firstly enabling the sperms of the Nibea albiflora to be subjected to the genetic inactivation by using ultraviolet irradiation, then activating the sperms and the ova by using seawater after the sperms are mixed with the ova of the Nibea albiflora, starting gynogenesis of the ova of the Nibea albiflora, restraining the discharge of a second polar body by using cold shock, doubling genomes of the ova, and inducing the Nibea albiflora gynogenesis diploid. The inducing method disclosed by the invention has the advantages that the operation is simple, the needed cost is lower, the practicability is stronger, and the like.

Owner:JIMEI UNIV

Inducing method for gynogenetic diploid of Nibea albiflora

ActiveCN102986571AEasy to operateHigh gynogenesis induction rateClimate change adaptationPisciculture and aquariaShock temperatureStart time

The invention provides an inducing method for a gynogenetic diploid of a Nibea albiflora, comprising the following steps of: (1) carrying out genetic inactivation on Nibea albiflora or Oplegnathus fasciatus sperms; (2) inseminating unfertilized ovums of the Nibea albiflora and inactivated sperms; (3) determining the starting time of chromosome reduplication of gynogenetic roes; and (4) screening the cold shock temperature and the duration of chromosome reduplication. The invention firstly provides a method for inducing Nibea albiflora ovum gynogenesis by using the sperms of the Nibea albiflora and the sperms of the Oplegnathus fasciatus to obtain a gynogenetic diploid fish fry, and the proper cold shock starting time, the cold shock temperature and the duration for Nibea albiflora ovum gynogenesis are screened out. The inducing method has the characteristics of convenience in operation, high efficiency, practicability and reliability; and a Nibea albiflora progeny obtained through inducing by using the method only contains the genetic materials of a female parent, so that the purification of an excellent gene can be accelerated, the excellent hereditary character can be rapidly fixed, and the inducing method has an important application value and a favorable popularization and application prospect on the aspect of breeding and sex control research of the Nibea albiflora.

Inducing method for gynogenetic diploid of Nibea albiflora

Inducing method for gynogenetic diploid of Nibea albiflora

Inducing method for gynogenetic diploid of Nibea albiflora

Owner:MARINE FISHERIES RES INST OF ZHEJIANG

Artificial spawning induction and insemination method for nibea albiflora

ActiveCN110169377ANot easy to oxidize and deteriorateIncrease contentClimate change adaptationPisciculture and aquariaSexual maturityOospore

The invention belongs to the technical field of biology and provides an artificial spawning induction and insemination method for nibea albiflora. The method includes: in a nibea albiflora parent breeding period, feeding nibea albiflora parents with a prepared stable composition, and performing nutrition enhancement; after sexual maturity of the parents, artificially collecting spawns and semen tocomplete insemination and obtain inseminated spawns; hatching the inseminated spawns, and carrying out fry breeding. Before artificial spawn and semen collection, injecting the nibea albiflora parents with spawning induction hormones is avoided. By adoption of the artificial spawning induction and insemination method, the spawns and semen can be regulated to realize synchronous maturity finally,so that nonsynchronous and unsmooth spawning and semen discharging can be avoided, and after-spawning parent fish death loss is avoided; large spawning quantity of female fish and high semen quality of male fish are realized, and the hatching rate and the effective hatching quantity can be increased so as to increase fry output; the composition is stable and easy to preserve and is capable of increasing the fat, protein and total sugar accumulation in testes and ovaries to enable synchronous development and maturity of testes and ovaries of the parents, and the spawning amount of parental female fish is increased.

Artificial spawning induction and insemination method for nibea albiflora

Artificial spawning induction and insemination method for nibea albiflora

Artificial spawning induction and insemination method for nibea albiflora

Owner:ZHEJIANG OCEAN UNIV

Feed additive for preventing and treating nibea albiflora enteritis disease

InactiveCN104322920AAnti-inflammatoryEasy to processDigestive systemAnimal feeding stuffDiseaseAnimal science

A feed additive for preventing and treating nibea albiflora enteritis disease consists of following components, by weight, 2-8 parts of cortex magnoliae officinalis, 15-30 parts of malt, 5-15 parts of sophora flowers, 10-20 parts of semen platycladi, 2-8 parts of semen cuscutae, 5-15 parts of bletilla striata, 10-20 parts of vervain and 4-12 parts of cyrtomium fortunei. A preparation method of the feed additive for preventing and treating the nibea albiflora enteritis disease comprises following steps: weighing the components according to the weight proportions and crushing and mixing the weighed components. When being used, the feed additive is added to a feed according to an adding amount of 5-6 g/kg.

Feed additive for preventing and treating nibea albiflora enteritis disease

Owner:ZHEJIANG OCEAN UNIV

Cage culture device for nibea albiflora capable of automatic feeding

InactiveCN104904639AReduce workloadControl the amount of baitClimate change adaptationPisciculture and aquariaWater dischargeMotor drive

The invention provides a cage culture device for nibea albiflora capable of automatic feeding. The cage culture device comprises a culture pond with a water inlet pipe and a water discharge pipe, a net cage and an oxygen-enriching unit. A support frame is arranged on the culture pond. A rotary shaft is arranged in the support frame. A feeding device is arranged above the net cage and comprises a material bin, a flap wheel, a servo motor and a discharge port. The feeding device is fixed onto the rotary shaft. An arranging unit is arranged on the support frame. The outer wall of the culture pond is provided with a control box. The cage culture device for nibea albiflora capable of automatic feeding has following beneficial effects: in the way that the servo motor drives one flap wheel to rotate for feeding, the amount of baits for feeding can be precisely controlled; flexible control is performed by arranging different feeding periods and amount of feeding baits on a touch screen according to different growth periods of nibea albiflora such that the amount of baits in the net cage is maintained at the proper range suitable for growth of nibea albiflora; and workload of workers is reduced and efficiency is increased.

Cage culture device for nibea albiflora capable of automatic feeding

Cage culture device for nibea albiflora capable of automatic feeding

Cage culture device for nibea albiflora capable of automatic feeding

Owner:杨丽花

Vitamin premix for nibea albiflora richardson juvenile fish, and preparation method of vitamin premix

InactiveCN106942522AImprove growth performanceImprove survival rateClimate change adaptationAccessory food factorsWeight gainingJuvenile fish

The invention provides a vitamin premix for nibea albiflora richardson juvenile fish, and a preparation method of the vitamin premix. The vitamin premix which is required by the nibea albiflora richardson juvenile fish during growth is developed according to the characteristics of nibea albiflora richardson, and is matched with the feed for use. Compared with the prior art, practical culture experiment using the feed which is prepared according to a formula of the disclosed vitamin premix for larimichthys crocea, and the vitamin premix for nibea albiflora richardson juvenile fish, which is provided by the invention, prove that the vitamin premix provided by the invention has the beneficial effects that the growth performance and the survival rate of the nibea albiflora richardson, and the feed utilization capacity can be significantly improved, and the blood and respiratory burst capability of the nibea albiflora richardson is improved. According to practical measurement results, the survival rate of the nibea albiflora richardson juvenile fish is as high as 100%; the weight gain rate is as high as 315%; the feed coefficient is 1.54; and the blood NBT vitality is 0.39.

Vitamin premix for nibea albiflora richardson juvenile fish, and preparation method of vitamin premix

Owner:MARINE FISHERIES RES INST OF ZHEJIANG

Chinese herbal medicine formula for treating enteritis of nibea albiflora seeds and application methods thereof

InactiveCN102166321AEnteritis disease controlPrevent residual effectsDigestive systemPlant ingredientsSide effectAdemetionine

The invention discloses a traditional Chinese medicine formula for treating enteritis of nibea albiflora seeds and application methods thereof, belonging to the field of agricultural marine culture. The formula is prepared from radix scutellariae, rhubarb, phellodendron bark, dangshen, hawthorn and malt according to the weight ratio of 2:5:3:2:1:1. The invention discloses two preparation methods, wherein one preparation method is used for preparing a full-pond sprinkling decoction of which the volume is metered according to the weight of Chinese herbal medicines in the decocting process; and the other preparation method is used for preparing an oral powder by grinding. The application methods are as follows: (1) the dose of the full-pond sprinkling decoction is 20ml/cubic water body; (2) the oral powder is added into the compound feed according to the weight ratio of 2%, the compound feed is fed 6 times per day, and the feeding quantity per day is 3-5g/10000 larval fishes; and (3) the decoction strengthens the biological bait, the decoction is added into a rotifer and artemia strengthened culture bucket according to the dose of 40ml/cubic water body, and collecting and cleaning processes are carried out after 8-10 hours. After the three methods are used for 5 days, the enteritis of the original seeds is controlled, the excrement dragging phenomenon does not occur, and the seeds recover. The traditional Chinese medicine formula is convenient to use, is economical and does not have side effects.

Owner:MARINE FISHERIES RES INST OF ZHEJIANG

Box-type Nibea albiflora fingerling transportation device suitable for long-distance transportation

ActiveCN106719265AEasy to operateImprove operational efficiencyPisciculture and aquariaEngineeringOxygen

The invention discloses a box-type Nibea albiflora fingerling transportation device suitable for long-distance transportation, and aims at providing a box-type Nibea albiflora fingerling transportation device which is convenient to operate and high in operation efficiency, and the problem that fingerling survival rates are influenced due to the fact that transparent nylon bags are not convenient to fix and are easy to shake during the transportation process can be effectively solved. The box-type Nibea albiflora fingerling transportation device comprises a transport vehicle; a mounting rack arranged on the transport vehicle, wherein the mounting rack comprises a vertical guide rod and a supporting platform elevating along the vertical guide rod; and a plurality of fingerling temporary rearing devices. A fingerling temporary rearing device comprises a temporary rearing barrel with an open-ended upper end; a plugging cover body cooperated with the corresponding temporary rearing barrel, wherein an outside surface of the plugging cover body is provided with a first sealing ring; and a first vertical guide sleeve arranged on the supporting platform. A connecting guide rod is disposed in each first vertical guide sleeve, and a lower end of the connecting guide rod is connected with the central section of an upper end surface of the corresponding plugging cover body. Each plugging cover body is provided with an exhaust vent and an oxygen charging hole which penetrate upper and lower end surfaces of the plugging cover body.

Box-type Nibea albiflora fingerling transportation device suitable for long-distance transportation

Box-type Nibea albiflora fingerling transportation device suitable for long-distance transportation

Owner:MARINE FISHERIES RES INST OF ZHEJIANG

Isolated culture and induction method of nibea albiflora precursor adipose cells

ActiveCN109182256AOptimize enzymatic digestion methodCell dissociation methodsCulture processDigestion TreatmentCell culture media

The invention relates to the technical field of cell culture and discloses isolated culture and an induction method of nibea albiflora precursor adipose cells. The isolated culture comprises the following steps: 1) firstly, digesting nibea albiflora adipose tissue blocks by utilizing a pancreatin solution; then treating the nibea albiflora adipose tissue blocks by utilizing a type II collagenase digestion solution; and 2) inoculating dissociated nibea albiflora precursor adipose cells into a cell culture bottle, and culturing by adopting a precursor adipose cell culture medium to obtain a cellsingle layer. The induction method comprises the following steps: after carrying out digestion treatment on the obtained nibea albiflora precursor adipose cells through the pancreatin solution, inoculating into a 6-pore plate and culturing; and then transferring into a precursor cell induction culture medium and continually culturing. According to the isolated culture and the induction method, the nibea albiflora precursor adipose cells can be successfully obtained and are further induced to obtain mature adipose cells; the isolated culture and the induction method have the advantages of simple and feasible method, good repeatability, low cost and rapid cell growth; and an effective in vitro cell model can be provided for molecular cell biology researches of nutrition metabolism of nibeaalbiflora.

Isolated culture and induction method of nibea albiflora precursor adipose cells

Isolated culture and induction method of nibea albiflora precursor adipose cells

Isolated culture and induction method of nibea albiflora precursor adipose cells

Owner:MARINE FISHERIES RES INST OF ZHEJIANG

Compound feed capable of promoting growth of nibea albiflora

InactiveCN107373208APromote absorptionFast growthFood processingClimate change adaptationDiseaseBiotechnology

The invention discloses compound feed capable of promoting growth of nibea albiflora. The compound feed is prepared from raw materials in parts by weight as follows: 25-45 parts of plant-source protein feed, 60-80 parts of wheat, 10-20 parts of blood worm powder, 4-8 parts of fermented feather powder, 20-40 parts of pine needle powder, 8-16 parts of fermented straw, 10-20 parts of yeast protein meal, 2-4 parts of montmorillonite, 2-4 parts of monocalcium phosphate, 18-22 parts of a digestion promoter and 3-6 parts of sweet potato powder. The proposed compound feed capable of promoting growth of nibea albiflora can effectively improve intestinal flora of the nibea albiflora, promote digestive absorption and strengthen the physique and realizes the effect on preventing diseases; the nibea albiflora has good absorption effect and grows fast.

Owner:合肥申仁养殖有限公司

Clostridium butyricum CG3 with probiotic activity and culture method and application thereof

ActiveCN110004094AImprove growth performanceIncrease intestinal SCFAs contentBacteriaAnimal feeding stuffSynbioticsGut flora

The invention relates to clostridium butyricum CG3 with probiotic activity and a culture method and application thereof. The clostridium butyricum CG3 is preserved in the China Type Culture Collection, and the preservation number is CCTCC NO. M2019071. The clostridium butyricum CG3 and synbiotics thereof significantly improve the growth performance of fishes, improve intestinal digestive enzyme activity, change the composition and diversity index of intestinal flora, enrich many SCFA production bacteria, and increase the content of fish intestinal SCFAs. In addition, a light nibea albiflora immune system can be activated by regulating serum complement and cytokine levels, lysozyme activity and intestinal antioxidant capacity, and the clostridium butyricum CG3 is non-hemolytic, sensitive tomost antibiotics, non-drug resistant and highly safe, and can be used as a feed additive for the light nibea albiflora to promote growth and prevent diseases.

Clostridium butyricum CG3 with probiotic activity and culture method and application thereof

Clostridium butyricum CG3 with probiotic activity and culture method and application thereof

Clostridium butyricum CG3 with probiotic activity and culture method and application thereof

Owner:SHANTOU UNIV

Method for culturing nibea albiflora

InactiveCN104996343AReduce pollutionReduce the risk of farmingClimate change adaptationPisciculture and aquariaAfter treatmentFresh water organism

A method for culturing nibea albiflora comprises steps as follows: male parent nibea albiflora and female parent nibea albiflora are mixed and cultured together, and fertilized eggs of the nibea albiflora are obtained; the fertilized eggs of the nibea albiflora are hatched with a loop incubator, and fries of the nibea albiflora are obtained; after a culturing system is sterilized, the culturing system is washed and soaked with fresh water; natural seawater is treated to meet the preset culturing index, and culturing seawater is obtained and injected into a culturing system; the fries of the nibea albiflora are put in the culturing system for culture, fish bait is put in the culturing system, pest control is performed, and the water quality of the culturing seawater is monitored in real time; the culturing wastewater is treated to meet the preset culturing index and to serve as the recyclable culturing seawater; the culturing seawater is supplemented and injected into the culturing system. The method for culturing the nibea albiflora is more environment-friendly and has smaller pollution on the environment; besides, the natural seawater meeting the preset culturing index after treatment is used for culturing the nibea albiflora, the culturing risk is lower, the culturing period is shorter, and the culturing quality is better.

Method for culturing nibea albiflora

Owner:张晶

Nibea albiflora breeding method

InactiveCN105961233ABreeding method is simpleImprove survival rateFood processingClimate change adaptationWater qualityObserved Survival

The invention discloses a Nibea albiflora breeding method, characterized by comprising the following steps: A, selecting a pond; B, treating the pond; C, putting Nibea albiflora fries; D, putting bait; E, monitoring water quality; F, performing medicated bath at regular periods. An objective of the invention is to overcome defects in the prior art, and provide the Nibea albiflora breeding method. The Nibea albiflora can be cultured artificially, and survival rate is high, feeding is convenient, bait putting dispersibility is good, and the method provides convenience for Nibea albiflora to eat.

Nibea albiflora breeding method

Nibea albiflora breeding method

Nibea albiflora breeding method

Owner:ZHONGSHAN ZHINING BIOTECH CO LTD

Nibea albiflora fry quick rearing method

InactiveCN105532541AImprove survival rateGood energyClimate change adaptationPisciculture and aquariaObserved SurvivalNibea albiflora

The invention provides a nibea albiflora fry quick rearing method and belongs to the technical field of aquaculture. La3+ elements are added into fry rearing water 15 days after the time from roe distribution to fry hatching, and the concentration of La3+ reaches 0.5-1.0 mg/L. An improvement is carried out on the basis of a conventional nibea albiflora fry rearing method, the lanthanum elements of a certain concentration are added into the fry rearing water, fries which quickly grow are obtained, and meanwhile the fry rearing survival rate is increased.

Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Method for improving gelling performance of minced Tilapia mossambica products

InactiveCN101861992BGood gelHigh gel strengthFood preparationTilapiaNibea albiflora

The invention discloses a method for improving the gelling performance of Tilapia mossambica products. In the method, minced Tilapia mossambica and minced Nibea albiflora are used as raw materials and are mixed in a certain proportion to prepare mixed minced fish. The gelling performance of the minced fish products made of the mixed minced fish is higher than that of a minced fish product (fish ham sausages, fried fish balls and fish cakes) made of the Tilapia mossambica or Nibea albiflora alone. The method provided by the invention can be used for making minced fish products such as the fishham sausages, fried fish balls and fish cakes.

Method for improving gelling performance of minced Tilapia mossambica products

Owner:SOUTH CHINA UNIV OF TECH

Quick label-hanging marking method of Japan Nibea albiflora Japomic and special reagent thereof

InactiveCN104206337AReduce harmEasy to operateClimate change adaptationPisciculture and aquariaDelta Aminolevulinic AcidNibea albiflora

The invention discloses a quick label-hanging marking method of Japan Nibea albiflora and a special reagent thereof. A treatment reagent consisting of 0.02 to 0.2 part of delta-aminolevulinic acid, 0.1 to 0.5 part of ms-222, 10 to 40 parts of NaCl (sodium chloride), and 10-60 parts of CaCl2 (calcium chloride) is adopted. Compared with the prior art, the special regent has the advantages that the operation is simple, the cost is low, the anesthesia effect is good, the action concentration is low, the performance of controllable recovery time is stable, and the falling rate of label is low.

Quick label-hanging marking method of Japan Nibea albiflora Japomic and special reagent thereof

Owner:MARINE FISHERIES RES INST OF ZHEJIANG

Method for improving fishing survival rate of nibea albiflora

InactiveCN101569294AClimate change adaptationPisciculture and aquariaMarine cultureFishing

The invention relates to a method for improving the fishing survival rate of a nibea albiflora (Richardson). The fished nibea albiflora is taken as the parent of artificial breeding. The invention belongs to the technical field of agriculture marine culture. The invention selects the nibea albiflora which is fished with longline fishing; the hook in the fished nibea albiflora is taken down in a container filling with water; an air tube (medical infusion venting needle tube) is used for puncturing into the belly of the fish from the base pectoral fin of the fish, wherein, the puncturing depth is from 0.5 cm to 1.0 cm according to the size of the fish; the belly of fish is lightly pressed to release air; and the air-released fish is put into a seawater container with 0.1 mg/kg oxytetracycline to have a medical bath and charge air for 1-2 hours. The survival rate of the nibea albiflora which is processed by the method can reach more than 90 %. The invention provides the parent of culturing seeding for artificially culturing the nibea albiflora.

Owner:MARINE FISHERIES RES INST OF ZHEJIANG +1

Method for identifying vitality of sperm of Nibea albiflora

InactiveCN101838680AReduce subjective factorsLarge number of detectable samplesMicrobiological testing/measurementColor/spectral properties measurementsATPaseCreatine kinase

The invention provides a method for identifying the vitality of the sperm of Nibea albiflora, relates to the technical field of aquaculture. The method uses a kit and an ultraviolet spectrophotometer, and is characterized in that: in the breeding season of the Nibea albiflora, a certain amount of sperm is collected by batch; and the activities of total ATPase, creatinekinase, succinodehydrogenase, lactic dehydrogenase and acid phosphatase in different batches of sperm are tested respectively by the kit to differentiate sperm with high vitality, sperm with medium vitality and sperm with low vitality. The method is used for identifying the vitality of the sperm of the Nibea albiflora.

Owner:EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Breeding method for decreasing feed coefficient of light-colored nibea albiflora and improving quality of swimming bladders

InactiveCN110393162AExpand the salinity range of farmingConducive to regional promotion of artificial breedingClimate change adaptationPisciculture and aquariaFisherySalinity

The invention discloses a breeding method for decreasing the feed coefficient of light-colored nibea albiflora and improving the quality of swimming bladders. According to the method, the salinity ofan original living water environment of light-colored nibea albiflora is taken as the initial salinity of the breeding water environment, the salinity of the breeding water environment is gradually lowered, the final salinity of the culture water environment is finally formed, and breeding of light-colored nibea albiflora continues in the breeding water environment with the final salinity. The breeding method can decrease the feeding amount of feed and increase the content of crude proteins and collagen in the swimming bladders while not influencing the growth of light-colored nibea albiflora,so that the feed coefficient of light-colored nibea albiflora is decreased, and the quality of the swimming bladders of light-colored nibea albiflora is improved.

Breeding method for decreasing feed coefficient of light-colored nibea albiflora and improving quality of swimming bladders

Breeding method for decreasing feed coefficient of light-colored nibea albiflora and improving quality of swimming bladders

Breeding method for decreasing feed coefficient of light-colored nibea albiflora and improving quality of swimming bladders

Owner:SHANTOU UNIV

Box-type transportation equipment for croaker fingerlings suitable for long-distance transportation

ActiveCN106719265BEasy to operateImprove operational efficiencyPisciculture and aquariaObserved SurvivalNibea albiflora

The invention discloses a box-type Nibea albiflora fingerling transportation device suitable for long-distance transportation, and aims at providing a box-type Nibea albiflora fingerling transportation device which is convenient to operate and high in operation efficiency, and the problem that fingerling survival rates are influenced due to the fact that transparent nylon bags are not convenient to fix and are easy to shake during the transportation process can be effectively solved. The box-type Nibea albiflora fingerling transportation device comprises a transport vehicle; a mounting rack arranged on the transport vehicle, wherein the mounting rack comprises a vertical guide rod and a supporting platform elevating along the vertical guide rod; and a plurality of fingerling temporary rearing devices. A fingerling temporary rearing device comprises a temporary rearing barrel with an open-ended upper end; a plugging cover body cooperated with the corresponding temporary rearing barrel, wherein an outside surface of the plugging cover body is provided with a first sealing ring; and a first vertical guide sleeve arranged on the supporting platform. A connecting guide rod is disposed in each first vertical guide sleeve, and a lower end of the connecting guide rod is connected with the central section of an upper end surface of the corresponding plugging cover body. Each plugging cover body is provided with an exhaust vent and an oxygen charging hole which penetrate upper and lower end surfaces of the plugging cover body.

Box-type transportation equipment for croaker fingerlings suitable for long-distance transportation

Box-type transportation equipment for croaker fingerlings suitable for long-distance transportation

Box-type transportation equipment for croaker fingerlings suitable for long-distance transportation

Owner:MARINE FISHERIES RES INST OF ZHEJIANG

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