Molecular marker for identifying genetic gender of nibea albiflora and application thereof

A technology of molecular markers and yellow croaker, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems that have not been reported, achieve accurate identification, improve aquaculture benefits, good stability effect

Active Publication Date: 2017-11-07
JIMEI UNIV
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Problems solved by technology

[0005] So far, molecular markers that can identify and identify (identify)

Method used

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  • Molecular marker for identifying genetic gender of nibea albiflora and application thereof
  • Molecular marker for identifying genetic gender of nibea albiflora and application thereof
  • Molecular marker for identifying genetic gender of nibea albiflora and application thereof

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Experimental program
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Embodiment

[0050] 1. Sex-specific molecular markers of yellow croaker

[0051] The results of transcriptome sequencing analysis of yellow croaker showed that the dmrt1 gene was highly expressed in male individuals, but almost not expressed in female individuals. From invertebrates to vertebrates, the dmrt1 gene plays a crucial role in sex determination. Based on the conservation of the function of the gene, the inventors considered using the dmrt1 gene in the yellow croaker as an entry point to carry out the mining of sex-specific molecules in the croaker. For this reason, the inventor designed primers on the exons of the croaker dmrt1 gene, and used its genomic DNA as a template to perform PCR amplification to obtain its intron sequence. Figure 7A . Through the comparison and analysis of the intron sequences of male and female croakers, it is found that there is a 45bp indel fragment in the No. 1 intron of the crocodile dmrt1 gene, see Figure 7B with 7C . Figure 7B It is a parti...

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Abstract

The invention discloses a molecular marker for identifying the genetic gender of nibea albiflora and application thereof. The molecular marker shows insertion/deletion length polymorphism of a nucleotide sequence shown as SEQ ID NO: 1; an individual having the nucleotide sequence shown as SEQ ID NO: 1 is a female nibea albiflora, and an individual without the nucleotide sequence shown as SEQ ID NO: 1 is a male nibea albiflora. The invention further discloses a primer pair, a kit and a method for detecting the molecular marker. The molecular marker can simply, conveniently, rapidly and stably identify the genetic genders of different individuals in each colony of the nibea albiflora, so that development of a unisexual breeding technology of the nibea albiflora and development of unisexual breeding of the nibea albiflora are facilitated, the breeding efficiency is further improved and nibea albiflora breeding economic benefits are increased; meanwhile, nibea albiflora gender determining mechanism and other relevant scientific researches are benefited.

Description

technical field [0001] The invention relates to fish genetic sex identification and sex control technology in the technical field of aquatic organisms, in particular to a molecular marker for identifying the genetic sex of yellow croaker and its application. Background technique [0002] In the field of life science research, gender research has always been a hot topic, attracting the attention of many researchers. Fish is in the position of connecting the past and the future in the evolution of animals, and the number of species is abundant. Similar to most vertebrates, many fishes are dioecious and sexually dimorphic, showing significant differences between males and females in morphology and physiology. Moreover, important economic traits such as individual growth and economic value of some fish species are closely related to sex; therefore, the development of parthenogenic breeding and breeding techniques for these fish species has great significance in the fish farming...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6879C12Q2600/156
Inventor 王志勇孙莎
Owner JIMEI UNIV
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