Method for developing SSR (Simple Sequence Repeat) molecular mark of nibea albiflora for population identification

A technology of molecular marker and identification method, which is applied in the field of population molecular genetics of yellow croaker, achieves the effects of reduced cost, low experimental cost, and stable and reliable method

Inactive Publication Date: 2016-06-01
JIMEI UNIV
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Problems solved by technology

At present, there is no report on the use of SSR molecular markers for population identification of yellow croaker based on the isolation of transcriptome gene sequences

Method used

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  • Method for developing SSR (Simple Sequence Repeat) molecular mark of nibea albiflora for population identification
  • Method for developing SSR (Simple Sequence Repeat) molecular mark of nibea albiflora for population identification
  • Method for developing SSR (Simple Sequence Repeat) molecular mark of nibea albiflora for population identification

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Embodiment Construction

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0031] see Figure 1~4 , in an embodiment of the present invention, a method for developing croaker SSR molecular markers for population identification, specifically comprising the following steps:

[0032](1) Yellow croaker samples: The samples of croaker croaker came from the breeding base of croaker croaker in Ningde, Fujian. In order to obtain the gene sequences of different stages, this experiment used different developmental stages (fertilized eggs, newl...

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Abstract

The invention discloses a method for developing an SSR (Simple Sequence Repeat) molecular mark of nibea albiflora for population identification. The method comprises the following steps: extracting RNA (Ribonucleic Acid) by using a nibea albiflora mixed tissue of a plurality of nibea albiflora, performing RNA sequencing, performing splicing to obtain a nibea albiflora expression gene sequence, and obtaining a potential SSR mark with a bioinformatics method; designing primers by utilizing an SSR flanking sequence, extracting genomic DNA (Deoxyribose Nucleic Acid) by using fins from a random sample, and screening the SSR primers; and performing PCR (Polymerase Chain Reaction) amplification on different nibea albiflora DNA templates by using each pair of the SSR primers, performing capillary electrophoresis detection on an amplification product by using a Qsep100 full-automatic nucleic acid analysis system, screening out primers with different amplification results, calculating allelic composition and allelic frequency by utilizing PopGene, and analyzing a nibea albiflora population relationship and a genetic structure. The SSR molecular mark method for identifying and verifying nibea albiflora groups, provided by the invention, is stable and reliable, is not influenced by ages, gender and growth seasons, and provides scientific bases for group analysis as well as species identification, breeding and evaluation of the nibea albiflora.

Description

technical field [0001] The invention relates to population molecular genetics technology of croaker, in particular to a method for developing croup SSR molecular markers for population identification. Background technique [0002] Yellow croaker (Nibea albiflora) belongs to Osteichthyes, Perciformes, Totoabaidae, and genus Nibea albiflora. It is an important seawater economic fish in my country, and it is a warm-temperate offshore middle-lower fish. my country's Yellow Sea, Bohai Sea, East China Sea and South China Sea are distributed. The artificial breeding technology of yellow croaker has been successful, and in recent years, the scale of breeding in Zhejiang and Fujian has continued to expand, and it has become an important economic fish for marine aquaculture in my country's coastal areas. Due to the pollution and destruction of the marine environment caused by overfishing and human activities, the fishery resources of yellow croaker have declined sharply, and wild res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 肖世俊龚诗琦谢仰杰王志勇
Owner JIMEI UNIV
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