Inducing method of Nibea albiflora gynogenesis diploid

A technique for gynogenesis and yellow croaker, which is applied to the induction of gynogenetic diploids of yellow croaker and the field of induction of fish gynogenesis, to improve the efficiency of gene homozygosity, the method is simple and easy, and avoid gene pollution Effect

Inactive Publication Date: 2013-12-11
JIMEI UNIV
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Problems solved by technology

However, the research on artificial gynogenesis of yellow croaker is still blank
Although there have been many kinds of fish artificial gynogenesis induction techniques reported at home and abroad, the sperm diluent required for different species, the irradiation dose of ul...

Method used

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Embodiment Construction

[0015] The invention is a method for inducing gynogenetic diploid of yellow croaker, which comprises the following steps:

[0016] 1. Genetic inactivation of croaker sperm. After diluting croaker semen 20-30 times with the diluent, pipette 3mL into a petri dish with a diameter of 9cm, so that the thickness of the semen is about 0.5-1mm; then place the petri dish on a tray filled with crushed ice and the surface is horizontal Then place the tray on a shaker with a rotation speed of 110 r / min; use two 30W UV lamps placed in parallel as light sources for irradiation (wavelength 254 nm), the irradiation distance is 40cm, and the UV-C intensity is 3800μw / ( cm 2 s), the irradiation time is 50-70s.

[0017] 2. Initiation of egg gynogenesis. Take 120 g of croaker eggs, add 15 mL of the genetically inactivated croaker semen mentioned in step 1, mix well, add seawater at 24±0.5°C, start gynogenesis of croaker eggs, and start timing.

[0018] 3. The chromosome set of the egg is doubl...

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Abstract

The invention discloses an inducing method of a Nibea albiflora gynogenesis diploid. The technical scheme of the invention is that the inducing method comprises the steps of genetic inactivation of sperms of Nibea albiflora and diploidzation of ova of the Nibea albiflora; the inducing method is mainly characterized by firstly enabling the sperms of the Nibea albiflora to be subjected to the genetic inactivation by using ultraviolet irradiation, then activating the sperms and the ova by using seawater after the sperms are mixed with the ova of the Nibea albiflora, starting gynogenesis of the ova of the Nibea albiflora, restraining the discharge of a second polar body by using cold shock, doubling genomes of the ova, and inducing the Nibea albiflora gynogenesis diploid. The inducing method disclosed by the invention has the advantages that the operation is simple, the needed cost is lower, the practicability is stronger, and the like.

Description

technical field [0001] The invention belongs to the field of aquaculture science and technology, and relates to a method for inducing fish gynogenesis, which uses genetically inactivated yellow croaker ( Nibea albiflora ) Sperm induces gynogenesis in fish eggs, especially a method for inducing diploid gynogenesis in yellow croaker. Background technique [0002] Gynogenesis refers to the phenomenon that the egg develops into an individual only by its own genetic material. It requires the same or different sperm to enter the egg, but these sperm only play an exciting role and do not participate in the development. Embryo development is completely under the control of the female nucleus Therefore, the offspring obtained are all maternal traits, and the genotype is the same or slightly different from the mother parent. Artificially inducing gynogenesis is one of the most effective methods to accelerate the homozygosity of good genes in fish and quickly fix good genetic traits, ...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王志勇谢仰杰杨育凯陈庆凯
Owner JIMEI UNIV
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