36 results about "Enzymic degradation" patented technology

The present invention relates to chitin oligose preparing process, and is especially enzyme generating fermentation process to degrade chitin colloid for preparing bioactive chitin oligose. The preparation process has simple technological path, effective inhibition of further degradation of bioactive chitin oligose, reuse of degrading enzyme and other advantages, and is suitable for industrial production. The preparation process includes: culturing Aeromonascaviae strain to obtain fermented liquid, centrifugally separating the fermented liquid to obtain enzyme supernatant; preparing chitin colloid; compounding chitin colloid buffer solution in an enzyme reactor and adding abacterial enzyme liquid for enzymolysis to obtain enzymolyzed chitin oligose liquid; four stages of membrane separation to obtain concentrate and spray drying to obtain bioactive chitin oligose product.

The invention provides a method for producing galacto-mannan-oligosaccharide by the enzymic degradation of locust bean gum. The method is characterized in that: preparing enzyme-producing culture medium, inoculating bacillus alcalophilus, fermenting, extracting coarse enzyme liquid of mannase, dissolving the locust bean gum in buffer solution, adding the coarse enzyme liquid of the mannase into the solution for enzymatic degradation, inactivating the mannase by boiling water bath heating, removing deposited impurities after centrifuging, making the precipitation fractionation by organic solvent after concentration, and obtaining the galacto-mannan-oligosaccharide fragment after freeze drying. The preparation method has the advantages of low requirement on environmental equipment, simple and easy operation, low cost, simple composition of the product, mild preparation conditions, good structural stability of the product and suitability for popularization.

The invention relates to the field of K-carrageenan oligosaccharides and particularly relates to a preparation method of K-carrageenan oligosaccharide having antioxidant activity. The preparation method comprises preparation of K-carrageenase, preparation of K-carrageenan oligosaccharide, determination of a hydroxyl free radical removal capacity of K-carrageenan oligosaccharide, and determination of a superoxide anion removal capacity of K-carrageenan oligosaccharide. Compared with the existing preparation method, the preparation method provided by the invention is simple and fast. Through use of enzymic degradation, the preparation method has the advantages of simple and fast process, high yield and stable quality. The product has antioxidant activity and has the prospect of developing a health food with an anti-oxidation function.

The invention discloses yeast peptone and a preparation method thereof. The preparation method comprises steps of pretreatment, size mixing, autolysis, enzymatic hydrolysis, centrifugation, membrane filtration and enrichment, and concentration and drying of a high protein yeast fermentation broth, wherein the molecular weight distribution of the yeast peptone is from 500Da to 3000Da. According to the preparation method, yeast serves as a raw material and the ultrafiltration technology is employed based on biological enzymic degradation; by the adoption of the membrane filtration and enrichment step and the optimization selection of the membrane flux and the concentration multiple, the molecular weight distribution of the peptone is precisely controlled and enabled to accord with standards; the percentage of the molecular weight distribution is maintained high; and the stability of product quality is guaranteed further. The preparation method is short in production cycle, low in cost, free of discharge of high-concentration organic waste water, more comprehensive in nutrition, stable, and easy in absorption and utilization.

A preparation method of heparin oligosaccharides comprises the following steps: 1, carrying out enzymic degradation on heparin: dissolving 20 mg of heparin in 1 ml of a reaction solution Digestion Buffer, adding 500 [mu]l of heparinase I, 500 [mu]l of heparinase II and 500 [mu]l of heparinase III, carrying out an oscillatory reaction for 72 h at a constant temperature of 37 DEG C with heparinase supplement every 12 h, heating in a water bath with the temperature of 100 DEG C for 15 min after ending the reaction, carrying out high speed centrifugation for 10 min with a speed of 12000 rpm, taking out a supernatant and setting aside; and 2, carrying out stage purification on heparin oligosaccharides: carrying out stage purification through passing oligosaccharide fragments obtained in step 1 through a Bio-gel P2 column, collecting and lyophilizing. So the finished products are obtained.

The invention discloses a tumor antigen TRAG-3 mimic epitope peptide and an application thereof. The mimic epitope peptide comprises an amino acid sequence shown by Ile-Leu-Leu-Arg-Asp-Ala-Gly-Leu-Val, wherein the fifth Asp is D-Asp or the sixth Ala is D-Ala. The mimic epitope peptide has the advantages of better enzymic degradation resistant stability compared with TRAG-3 natural epitope peptide, stronger appetency compared with HLA-A2.1, and stronger CTL specific killing effect compared with TRAG-3 positive tumor cells, can be used for preparing polypeptide vaccine for treating the TRAG-3 positive tumor cells, and has good development and application prospect in the immunotherapy filed.

There are provided a novel series of peptide analogs of hGH-RH(1-29)NH2 and hGH-RH(1-30)NH2 which show high activities in stimulating the release of pituitary GH in animals. They retain their physiological activity in solution for extended periods of time and resist enzymic degradation in the body. These novel and useful properties appear to be due to novel substitution patterns ant at the 1, 15, 27 and 29 positions on the peptide.

The invention relates to a method for preparing cassava polysaccharide iron. The method is to oxidize and degrade cassava starch with hydrogen oxide to obtain an applicable oligosaccharide solution, and then add ferric chloride and sodium hydroxide solution dropwise to perform a complex reaction to obtain cassava polysaccharide. The iron synthesis solution is then filtered to remove water-insoluble impurities to obtain cassava polysaccharide iron solution, which is then desalinated and concentrated by ultrafiltration membrane technology, spray dried or vacuum evaporated, dried and crushed, and finally cassava polysaccharide iron products are obtained. The present invention uses hydrogen peroxide to oxidize and degrade cassava starch to replace the previous acid or enzymatic degradation process, which not only can significantly reduce the generation of by-product glucose than the acid method, obtain a higher yield of applicable oligosaccharides, and is more efficient than the enzymatic method. The degradation time is greatly shortened, thereby reducing production costs; it also replaces the previous ethanol or methanol solvent method to remove sodium chloride, avoids the use of flammable and explosive materials such as ethanol or methanol, and eliminates hidden dangers in production safety. The cassava polysaccharide iron prepared by the invention can be used as an iron supplement for poultry and livestock.

The present invention discloses an extraction method for fucoidin with a high yield and a high ratio of fucose, belongs to the technology field of algae processing and utilization. According to the present invention, nemacystis is used as a raw material, a high-pressure homogenisation method is used to extract the fucoidin, and therefore extraction time is short, extraction efficiency is high, and retention of natural components content in the fucoidin is maximized. By combining a chemical degradation method and a enzymatic degradation method to degrade the fucoidin, and by ultrafiltration technology, finally low-molecular-weight fucoidin with outstanding anti-tumor activity is obtained. According to the present invention, the yield of the obtained fucoidin is 21%-34%, the purity is 85%-90%, the fucose content reaches 70%-75%, and the organic sulfate ion content reaches 20%-30%.

The invention relates to a method and application for preparing low-viscosity octenyl succinate konjac glucomannan ester by using β-mannanase. The present invention creatively uses natural non-toxic, non-polluting β-mannanase as raw material, uses it to enzymatically degrade konjac glucomannan, and then further esterifies and modifies the polysaccharide to obtain the β-mannanase of the present invention. Viscosity target product. The target product octenyl succinic acid konjac glucomannan ester obtained by the present invention has low viscosity, and the viscosity under 1wt% concentration condition can be as low as 2700mPa.s (25 ℃), therefore, can be used as an emulsifier to emulsify corn germ oil , broaden the scope of application of traditional konjac glucomannan ester. In addition, the present invention also applies microwave heating to the modification of KGM, which can achieve the effects of high efficiency, energy saving, emission reduction and consumption reduction. Moreover, compared with the solvent method of the traditional reaction, the reaction system of the present invention is in a semi-dry state, and there is no waste water discharge in the whole reaction process, which has a significant environmental protection effect.

The invention relates to the technical field of biology, in particular to arthrobacter aurescens and application of the arthrobacter aurescens in preparation of an okra polysaccharide degrading enzyme. The arthrobacter aurescens (TU) is preserved in China Center for Type Culture Collection on January eleventh, 2019, and the preservation number is CCTCC NO:M 2019035. The strain can efficiently secrete the okra polysaccharide degrading enzyme and can be applied to preparation of the okra polysaccharide degrading enzyme. The okra polysaccharide degrading enzyme generated by the strain has high okra polysaccharide degrading activity, high heat stability and wide temperature and pH adaption range, efficient resources are provided for okra polysaccharide degrading through an enzyme method, and an advantageous technical means is provided for high-value utilization of okra.

The invention relates to the field of K-carrageenan oligosaccharides, in particular to a method for preparing K-carrageenan oligosaccharides with antioxidant activity. It includes the preparation of K-carrageenan enzyme and the preparation of K-carrageenan oligosaccharide, and also includes the determination of the ability to scavenge hydroxyl radicals of K-carrageenan oligosaccharide and the determination of the ability to scavenge superoxide anion of K-carrageenan oligosaccharide method. The preparation method of the invention is simpler and quicker than the existing preparation methods. The invention adopts enzymatic degradation, so that the preparation process is simple and quick, the yield of the product is high, and the quality is stable, and the product has anti-oxidation activity, and has the prospect of developing health food with anti-oxidation function.

The invention relates to a chitooligosaccharide preparation method, relating to a chitooligosaccharide, in particular to a method for preparing biologically active chitin oligosaccharides by degrading chitin colloids by fermenting an enzymatic method. Provide a simple process route, which can effectively inhibit the further degradation of active chitosan oligosaccharides under the action of enzymes. The degrading enzyme can be reused, and the efficiency of enzyme use is high. Products with different degrees of polymerization can be obtained according to needs, with less pollution and high separation efficiency. , a chitosoligosaccharide preparation method capable of large-scale industrial production. Centrifuge and separate the fermentation broth obtained from the culture of Aeromonas caviae, obtain the supernatant of the fermentation broth, and separate the enzyme solution; then prepare chitin colloid; in the enzyme reaction tank, suspend the chitin colloid in the buffer solution , prepared into a chitin colloid solution, aseptically transporting the chitin enzyme solution to the chitin colloid solution in a sterilized enzyme reaction tank to obtain a chitin oligosaccharide enzymatic hydrolysis solution; The three and four stages of membrane separation spray-dry the intercepted concentrated solution to obtain the active chitosan oligosaccharide product.

The present invention relates to the application of 5'-monophosphate nucleotides and mixtures thereof in the preparation of medicines or foods for improving mitochondrial function. 5'-monophosphate nucleotides are produced by enzymatic degradation of ribonucleic acid as raw materials, and 5'-monophosphate 5'-monophosphate and 5'-uridylate disodium are more than 99% pure. The 5'-nucleotide mixture includes : CMP 23-78%, AMP 6-44%, UMP 7-40%, GMP 7-51%, IMP is 0, or greater than 0 and not higher than 2.5%. The present invention finds that 5'-monophosphate nucleotides and mixtures thereof can improve H 2 O 2 Mitochondrial function in induced senescent fibroblasts.

The invention discloses a tumor antigen TRAG-3 mimic epitope peptide and an application thereof. The mimic epitope peptide comprises an amino acid sequence shown by Ile-Leu-Leu-Arg-Asp-Ala-Gly-Leu-Val, wherein the fifth Asp is D-Asp or the sixth Ala is D-Ala. The mimic epitope peptide has the advantages of better enzymic degradation resistant stability compared with TRAG-3 natural epitope peptide, stronger appetency compared with HLA-A2.1, and stronger CTL specific killing effect compared with TRAG-3 positive tumor cells, can be used for preparing polypeptide vaccine for treating the TRAG-3 positive tumor cells, and has good development and application prospect in the immunotherapy filed.