Extraction method for fucoidin with high yield and high ratio of fucose

A technology of fucoidan and an extraction method, which is applied in the field of seaweed processing and utilization, can solve the problems of low quality of fucoidan, many impurities of fucoidan, insufficient quality for use, etc., and achieves environmental protection, mild extraction conditions, and high extraction efficiency. short time effect

Active Publication Date: 2015-10-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the traditional extraction methods of fucoidan, water extraction and acid extraction are the most common methods. However, the fucoidan obtained by the traditional extraction method has many impurities, low yield and low quality.
The quality of fucoidan sold in th...

Method used

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  • Extraction method for fucoidin with high yield and high ratio of fucose
  • Extraction method for fucoidin with high yield and high ratio of fucose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Direct extraction of fucoidan by high-pressure homogenization

[0039] Weigh 50g of dried mozuku, pulverize with a pulverizer, sieve through a 100-mesh sieve, add water according to the mass ratio of dried mozuku:water=1:30, soak for a period of time, stir well, and homogenize once under 15Mpa.

[0040] After high-pressure homogenization, centrifuge at 4000rpm for 15min, take the centrifuged supernatant (i.e. extract), concentrate the extract, add 3 volumes of absolute ethanol per L of extract, mix well, and let stand to obtain a precipitate . The precipitate is filtered and vacuum-dried to obtain refined fucoidan.

[0041] 15.48 g of fucoidan was obtained with a yield of 30.96% (product / raw material dry weight×100), a purity of 88.22%, and an organic sulfate content of 22.03%. The molar content of fucose in the fucoidan is 73.84%.

Embodiment 2

[0042] Example 2: Extraction of fucoidan by high pressure homogenization method combined with chemical degradation method (1)

[0043] Weigh 0.5kg of fresh mozuku (dry weight is about 20-25g), wash it, add 1.5kg of water (that is, the ratio of material to water is 1:60-1:75), and homogenize once at 20Mpa. Then centrifuge at 5000rpm for 10min, and take the centrifuged supernatant (ie extract). Concentrate the extract to 1 / 2 of the original volume, and then add 8 mL of 30% H 2 o 2 , 60°C water bath, degrade for 1h, adjust the pH of the degradation solution to neutral with 1M NaOH solution, and then add catalase to react for 20min. The degradation solution flows through ultrafiltration columns with a molecular weight cut-off of 30KD and 10KD, and the 10-30KD concentrated solution is collected to obtain a retentate. Add 4 times the volume of absolute ethanol to the retentate, mix well, and let it stand for 24 hours to collect solid sediment. ; The precipitate was filtered throu...

Embodiment 3

[0045] Example 3: Extraction of fucoidan by high pressure homogenization method combined with chemical degradation method (2)

[0046] Weigh 0.5kg of fresh mozuku (dry weight is about 20-25g), clean it, add 4kg of water (that is, the ratio of material to water is 1:160-1:200), and homogenize 3 times under 10Mpa. Centrifuge and take the supernatant (ie extract). Concentrate the extract to 1 / 4 of the original volume, and then add 5 mL of 30% H 2 o 2 , 50°C water bath, degrade for 4h, adjust the pH of the degradation solution to neutral with 1M NaOH solution, and then add catalase to react for 10min. The degradation solution flows through the ultrafiltration column with a molecular weight cut-off of 30KD and 10KD, and the concentrated solution of 10-30KD is collected to obtain the retentate, and 4 times the volume of absolute ethanol is added to the retentate, mixed, left standing, and the solid sediment is collected; The precipitate was filtered through a plate frame and drie...

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Abstract

The present invention discloses an extraction method for fucoidin with a high yield and a high ratio of fucose, belongs to the technology field of algae processing and utilization. According to the present invention, nemacystis is used as a raw material, a high-pressure homogenisation method is used to extract the fucoidin, and therefore extraction time is short, extraction efficiency is high, and retention of natural components content in the fucoidin is maximized. By combining a chemical degradation method and a enzymatic degradation method to degrade the fucoidin, and by ultrafiltration technology, finally low-molecular-weight fucoidin with outstanding anti-tumor activity is obtained. According to the present invention, the yield of the obtained fucoidin is 21%-34%, the purity is 85%-90%, the fucose content reaches 70%-75%, and the organic sulfate ion content reaches 20%-30%.

Description

technical field [0001] The invention relates to a method for extracting fucoidan with high yield and high proportion of fucose, and belongs to the technical field of seaweed processing and utilization. Background technique [0002] my country is rich in algae resources, especially brown algae resources, and is a big country in seaweed production and consumption. Brown algae are rich in polysaccharides, such as algin, laminarin, carrageenan, etc., which have become important food or medical raw materials and are widely used. It is distributed in my country, Okinawa, Japan, and along the coast. [0003] Fucoidan is an inherent intercellular polysaccharide in brown algae, which exists in the cell wall matrix. Modern studies have shown that fucoidan has more than 20 physiological effects such as anticoagulation, anti-tumor, anti-virus, anti-oxidation, promotion of skin regeneration, and skin moisturizing. Low-molecular-weight fucoidan has the most outstanding anti-tumor effect...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00
Inventor 余晓斌李国莹顾秋亚
Owner JIANGNAN UNIV
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