Composite material for degrading bisphenol A as well as preparation method and application of composite material

A composite material and bisphenol technology, applied in the fields of nanomaterials and water pollution prevention and control, can solve problems such as complicated operation, high cost, and secondary pollution, and achieve the effects of simple preparation, expanded application range, and improved stability

Pending Publication Date: 2022-04-29
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the concentration of BPA is lower than that of other common interfering substances, the cost of removing it to a safe range is high, the operation is complicated, and secondary pollution may...

Method used

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  • Composite material for degrading bisphenol A as well as preparation method and application of composite material
  • Composite material for degrading bisphenol A as well as preparation method and application of composite material
  • Composite material for degrading bisphenol A as well as preparation method and application of composite material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the preparation of carrier material CN and ACN

[0042] (1) Preparation of graphitic carbon nitride CN

[0043]CN was prepared by thermal polycondensation of urea: dry urea was ground into powder, and then placed in a muffle furnace at 550 °C for 4 h, with a heating rate of 2.5 °C / min. After natural cooling to room temperature, the yellow sample was acid-washed overnight in nitric acid solution (pH=1), then washed with deionized water until neutral, then centrifuged at 6000 r / min for 3-5 min, and dried at 60 °C for 24 h. CN available.

[0044] (2) Preparation of ACN

[0045] ACN is prepared by thermal polycondensation of a mixture of urea and 4-aminopyridine: 20 g of dry urea and 0.09 g of 4-aminopyridine are mixed and ground into powder, and the two are fully mixed, and then the mixture is placed in a muffle at 550 °C Keep in the furnace for 4 h with a heating rate of 2.5 °C / min. After naturally cooling to room temperature, the light yellow sample was...

Embodiment 2

[0048] Embodiment 2: the preparation of HRP / ACN immobilized enzyme

[0049] 0.3g of ACN was dispersed in 4mL of PBS, and dispersed evenly by ultrasonic. Then 0.7 mL of HRP solution with a concentration of 1 mg / mL was added dropwise to the above mixture, and the reaction was stirred at room temperature for 20 min. Finally, it was separated by centrifugation at 8000 r / min for 5 min, and the centrifuged sample was placed in a freeze dryer at -80 °C for 24 h to obtain the HRP / ACN composite material.

[0050] figure 2 In Figures a-c, CN (a), ACN 45 (b) and HRP / ACN 45 The SEM image of (c), the three materials under the SEM image all show a bulk structure, but the surface of ACN is relatively smoother than that of CN after doping with 4-aminopyridine, figure 2 In c, after ACN is loaded with HRP, its surface is relatively "smooth", with some changes but still not obvious.

[0051] Observation of changes in bright field and fluorescence field by laser confocal microscopy (LSC...

Embodiment 3

[0056] Embodiment 3: HRP / ACN immobilized enzyme preparation condition optimization

[0057] (1) The effect of ACN content on the solid loading capacity of HRP / ACN

[0058] Add different amounts of ACN (0.1–0.9 mg) to 4 mL of PBS buffer, and sonicate to form a suspension. Subsequently, 1 mL of HRP solution with a concentration of 1 mg / mL was added dropwise to the above mixture, and PBS buffer solution was added to ensure that the volume was the same as 5 mL. Stir the reaction at room temperature for 20 min, and centrifuge at 8000 r / min for 5 min to separate, test the content of HRP before and after immobilization, and obtain the effect of the amount of carrier material on the immobilization capacity of HRP.

[0059] Figure 5 Shown in a is the effect of different amounts of carrier materials on the immobilized amount of HRP. The results showed that when the amount of carrier material was 0.3 mg, the immobilized amount of HRP reached 708.67 mg / g, and then increased the carri...

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Abstract

The invention belongs to the technical field of nano materials and water pollution prevention and control, and relates to a composite material for degrading bisphenol A as well as a preparation method and application of the composite material. According to the invention, the HRP is immobilized on the ACN carrier material, so that the range of use conditions of the HRP is effectively expanded, and the stability of the HRP is remarkably improved. When the HRP/ACN immobilized enzyme is applied to degradation of bisphenol A, the reaction rate of degrading bisphenol A by an enzyme method is improved, and the immobilized enzyme has important significance on ecological environment and human health.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and water pollution prevention and control, and relates to a composite material for degrading bisphenol A and its preparation method and application, in particular to a novel optical drive nanomaterial-mediated immobilized enzyme cascade catalytic system HRP / Preparation method and application of ACN. Background technique [0002] Bisphenol A, also known as BPA, scientific name 2,2-di(4-hydroxyphenyl) propane, referred to as diphenol propane, is an important industrial organic compound, mainly used in the production of polycarbonate, epoxy resin, Polysulfone resin, polyphenylene ether resin and other polymer materials can also be used in fine chemical products such as plasticizers, flame retardants, antioxidants, heat stabilizers, rubber anti-aging agents, pesticides, coatings, etc. Bisphenol A Heavy use presents many environmental and safety concerns. How to reduce this hazard, and by wha...

Claims

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Application Information

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IPC IPC(8): C12N9/08C12N11/14A62D3/02A62D101/28
CPCC12N9/0065C12N11/14A62D3/02C12Y111/01007A62D2101/28
Inventor 贺婷王龙飞吴嘉聪王赟韩娟毛艳丽王蕾李春梅
Owner JIANGSU UNIV
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