Thermophilic esterase and application thereof in degradation of PAEs (Phthalic Acid Esters)

A technology of esterase and biological enzyme, applied in the field of bioengineering, can solve the problems of little practical application value and poor stability, and achieve the effects of superior thermal stability, fast reaction speed and good stability of degradation reaction
CN104152425AInactive Publication Date: 2014-11-19EAST CHINA UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Publication Date
2014-11-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a thermophilic esterase and an application thereof in degradation of PAEs (Phthalic Acid Esters). The amino acid sequence of the thermophilic esterase is shown in SEQ ID NO.1. A hydrolysis reaction of PAEs is carried out in a water-containing medium under the catalysis of the thermophilic esterase and the fusion protein of the thermophilic esterase so as to generate a phthalic monomer acid and an alcohol are generated, and a phthalic acid and the alcohol are further formed. The thermophilic esterase can be used for preliminarily degrading a plasticizer, has a certain resistance to a denaturant and an organic solvent with moderate and low concentrations, has excellent heat stability and has the function of degrading various PAEs. The thermophilic esterase is the only one esterase cloned from thermophilic microorganisms according to literature reports. The thermophilic esterase has a good application prospect in the purely enzymatic degradation of the PAEs on the basis of the excellent performance. The thermophilic esterase realizes harmless degradation of the PAEs, and is good in degradation reaction stability, extensive in reaction condition and high in reaction speed.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, in particular to a thermophilic esterase and its application in degrading PAEs. Background technique

[0002] Various chemical synthetic substances play a vital role in human life, they improve people's life and promote the development of human social civilization. But at the same time, they also pollute our earth and bring great pressure to our living environment. There are also some compounds whose harm to the environment has been verified after years of scientific experiments. At this time, their accumulation range and accumulation amount in nature have reached a serious level. Phthalate esters (PAEs), also known as phthalates, are a general term for about 30 compounds that are widely used in daily life and are mainly used as plasticizers for plastic products. They are reproductively toxic at very low concentrations , and after it enters the environment, it is not easy to degrade rapi...

Claims

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