Quercetin crosslinking method for preparing artificial bioprosthesis heart valve materials
A heart valve, quercetin technology, applied in the field of biomedical engineering, can solve problems such as fragility, affecting the working period of biological valves, and becoming brittle
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Embodiment 1
[0056] Use the balanced salt buffer D-Hanks solution at 1-6°C as the storage and transportation solution, take the fresh porcine aortic heart valve from the slaughterhouse, cut the valve leaflets at 4°C within 4 hours, and use D-Hanks Fluid clean. Afterwards, the decellularized porcine heart aortic valve was prepared according to the aforementioned method, washed and washed, and the decellularized valve material with the structure and shape shown in Figure 1 was obtained. Then add 2ml of quercetin to each valve to soak in ethanol-containing D-Hanks special solution, shake and cross-link at 120rpm at 37°C for 48 hours. Add 2ml of D-Hanks solution to each valve to soak the valve, shake and wash at 120rpm at 37°C for 48 hours, and repeat the washing 3 times. The obtained biological valve material was evaluated for the above performance. 2 in Figure 2 is a decellularized valve cross-linked with 6.25 mg / ml glutaraldehyde, which is as loose as the non-cross-linked decellularized v...
Embodiment 2
[0058] Prepare the decellularized porcine heart aortic valve according to the method described in the summary of the invention and clean it, add 2.5mg / ml quercetin-containing D-Hanks special solution with ethanol at a ratio of 2ml per valve. Example 1 Method Cross-linking, the resulting cross-linked biological valve material can also maintain the original shape of the valve, soft and moist without shrinking. Figure 3 shows that the maximum tensile strength of the decellularized valve cross-linked by 2.5 mg / ml quercetin is 13.9 MPa. Figure 5 shows that only 28.5% of the decellularized valve cross-linked with 2.5 mg / ml quercetin was degraded after 24 hours of enzymatic hydrolysis. It shows that this concentration of quercetin also has a cross-linking effect on the decellularized valve, and has good mechanical properties and stability against enzymolysis.
Embodiment 3
[0060] Get fresh porcine aortic heart valve from slaughterhouse by the method of embodiment 1 and clean up. After washing with D-Hanks solution three times, valve cells were removed by shaking with decellularization solution at 60 rpm at 4°C for 96 hours. Use D-Hanks solution to shake the decellularized heart valve material at 21°C (room temperature) at 120rpm to wash valve cell residues, debris, free protein, nucleic acid and other macromolecules for 96 hours. Add 0.1mg / ml, 0.2mg / ml, 0.5mg / ml, 2mg / ml and 5mg / ml quercetin in ethanol-containing D-Hanks special solution at a rate of 20ml per valve, at 20°C, at 240rpm shaking speed, cross-linked for 96 hours. Add 20ml of D-Hanks solution to each valve to soak the valve, and wash at 120rpm for 10 minutes at room temperature. The bioprosthetic valve material of the present invention that makes like this is done aforementioned performance evaluation, and its maximum tensile strength is respectively 10.4MPa, 12.5MPa, 14.4MPa, 14.9M...
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