Preparation method of K-carrageenan oligosaccharide having antioxidant activity

A carrageenan oligosaccharide, antioxidant activity technology, applied in biochemical equipment and methods, preparation of test samples, microorganism-based methods, etc. The preparation process is simple and fast, and the quality is stable.

Active Publication Date: 2017-07-28
QINGDAO BZ OLIGO BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are reports on enzymatic hydrolysis of carrageenan oligosaccharides, but there is no report on enzymatic K-carrageenan oligosaccharides with antioxidant activity

Method used

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  • Preparation method of K-carrageenan oligosaccharide having antioxidant activity
  • Preparation method of K-carrageenan oligosaccharide having antioxidant activity
  • Preparation method of K-carrageenan oligosaccharide having antioxidant activity

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Embodiment Construction

[0029] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments.

[0030] A preparation method of K-carrageenan oligosaccharides with antioxidant activity specifically comprises:

[0031] One, the preparation of K-carrageenase

[0032] 1. Inoculate the screened Cellulophagalytica strain N5-2 in the activation medium, and culture it on a shaker at 20-30°C and 150-250rpm / min for 15-20h, and then activate it to obtain a seed solution.

[0033] 2. Inoculate the seed liquid into the fermentation medium according to the inoculum amount of 2-4%, and culture on a shaker at 20-30°C and 150-250rpm / min for 15-20h.

[0034] 3. Refrigerate and centrifuge the fermentation broth at 4000-5000r / min for 20min at 4°C, and take the supernatant after centrifugation, which is the crude enzyme solution.

[0035] 2. Preparation of K-carrageenan oligosaccha...

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Abstract

The invention relates to the field of K-carrageenan oligosaccharides and particularly relates to a preparation method of K-carrageenan oligosaccharide having antioxidant activity. The preparation method comprises preparation of K-carrageenase, preparation of K-carrageenan oligosaccharide, determination of a hydroxyl free radical removal capacity of K-carrageenan oligosaccharide, and determination of a superoxide anion removal capacity of K-carrageenan oligosaccharide. Compared with the existing preparation method, the preparation method provided by the invention is simple and fast. Through use of enzymic degradation, the preparation method has the advantages of simple and fast process, high yield and stable quality. The product has antioxidant activity and has the prospect of developing a health food with an anti-oxidation function.

Description

technical field [0001] The invention relates to the field of K-carrageenan oligosaccharides, in particular to a method for preparing K-carrageenan oligosaccharides with antioxidant activity. Background technique [0002] As a natural marine sulfated polysaccharide with unique structure, carrageenan is called heparan-like polysaccharide, and its biological activity has become a hot spot in the field of polysaccharide research. In recent years, the biological activity of carrageenan in physiological and pathological research has been discovered one after another. Experimental studies have shown that carrageenan has biological activities such as anti-angiogenesis, anti-tumor, immune regulation, anti-oxidation, antibacterial, anti-virus, and hypoglycemic. However, due to the large molecular weight of carrageenan polysaccharide, poor solubility and absorption, complex structure and other reasons, its application is limited. The molecular weight of carrageenan oligosaccharides i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/38C12P19/14G01N21/31G01N1/38G01N30/02G01N30/06C12R1/01
CPCC12N9/2468C12P19/14C12Y302/01083G01N1/38G01N21/314G01N30/02G01N30/06
Inventor 王丽丽张斌管昶徐小龙高芹芹岳帆
Owner QINGDAO BZ OLIGO BIOTECH
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