47 results about "Enzyme action" patented technology

The invention discloses a dynamic self-adaptive control method for a mechanical arm holder, which belongs to the technical field of PID (Proportion Integration Differentiation) control optimization, and specifically comprises the following steps: step 1, constructing a mechanical arm holder control system; step 2, improving an enzyme action optimization algorithm; step 3, obtaining an error between the current position and the target position of the mechanical arm holder, calculating an angle error of the holder needing to be adjusted, and optimizing control parameters of a PID controller module of the mechanical arm holder control system through an improved enzyme action optimization algorithm to obtain a group of optimal control parameters; step 4, inputting a group of optimal control parameters obtained in the step 3 into a PID controller, and adjusting the mechanical arm holder to reach a target angle through the control quantity output by the PID controller; a PID controller in the mechanical arm holder control system is optimized through an improved enzyme action optimization algorithm, and the adaptive capacity and the control precision of the mechanical arm holder control system are improved.

The invention belongs to the technical field of grease preparation, and particularly relates to a preparation method and application of diglyceride oil with a weight reducing effect. Comprising the following steps: firstly, adding grease into a basic nutrient solution, adding strains for fermentation, and filtering to obtain a glycolysis product; adding glycerol into the glycolysis product, carrying out lipase esterification reaction, and carrying out centrifugal separation to obtain an oil phase; the method comprises the following steps: degrading triglyceride by adopting dominant strain fermentation, then adding glycerol, and carrying out esterification reaction under the action of dominant compound lipase to obtain the diglyceride oil. According to the method, the 1, 3-diglyceride can be selectively generated, the proportion of dominant grease of the 1, 3-diglyceride can be increased, and research shows that the prepared diglyceride oil has good weight reduction and fat reduction effects and has a good application prospect.

The invention discloses a method for simultaneously determining gene point mutation and DNA methylation based on a CRISPR / Cas12a system. The method is characterized by comprising four steps of enzyme digestion, recognition, conversion and detection, wherein in the enzyme digestion step, methylation-sensitive restriction endonuclease (MSRE) is selected according to a target site sequence, unmethylated DNA is not hydrolyzed by MSRE and is kept complete, methylated DNA is hydrolyzed and broken under the action of MSRE, and the broken 3'end can be used as a primer P1 to trigger SDA1 amplification; wherein in the recognition step, a Flap primer and an Invading primer are designed according to a point mutation sequence, and an enzyme digestion product generated under the action of FEN1 enzyme can be used as a primer P2 to trigger SDA2 amplification; wherein in the conversion step, two different SDA amplification templates are designed according to sequences of P1 and P2, and amplification of SDA1 and SDA2 is triggered by enzyme digestion products P1 and P2 respectively, so that DNA methylation and gene mutation information are quickly converted into two different amplification products T1 and T2; wherein in the detection step, crRNA1 and crRNA2 are respectively designed as guide chains of a CRISPR / Cas12a reaction system according to sequences of T1 and T2, and synchronous detection of gene point mutation and DNA methylation is realized through'strong 'and'weak' of fluorescence signals. Based on a CRISPR / Cas12a system, the method solves the problem that a traditional detection method cannot realize simultaneous detection of gene point mutation and DNA methylation, can be used for scientific research analysis and clinical detection, and has a wide market prospect.

To provide a glycan preparation method for preparing labeled glycans quickly from glycoproteins.SOLUTION: A glycan preparation method comprises: a releasing step in which a glycosidase is allowed to act on a glycoprotein immobilized on a solid phase in a container to obtain a released product containing glycans; and a labeling step in which a labeling reagent is added to the released product in the container to obtain a labeled product containing labeled glycans, wherein the glycoprotein is an antibody, the solid phase has on its surface a ligand selected from the group consisting of protein A, protein G, protein L, protein H, protein D, and protein Arp, and the labeling reagent comprises a reducer, a solution and at least one selected from the group consisting of 8-aminopyrene-1,3,6-trisulfonic acid, a sodium salt thereof, and 9-aminopyrene-1,4,6-trisulfonic acid.SELECTED DRAWING: None

The invention relates to a circular DNA probe for recognizing vibrio vulnificus and a preparation method of the circular DNA probe, specifically, a linear probe carrying DNAzyme is mixed with a looping primer, the mixture is connected under the action of T4DNA ligase to form the circular DNA probe, the circular DNA probe can be specifically recognized with a target and then is subjected to rolling circle amplification to form long-chain DNA, the long-chain DNA contains a large amount of DNAzyme formed by amplification and repeated GC-rich regions, and the long-chain DNA can be used for detecting the vibrio vulnificus. Intramolecular or intermolecular hydrogen bonds are spontaneously formed and are self-assembled to form the DNA hydrogel in a porous state, so that the structure promotes region limitation, and the detection sensitivity is greatly improved; target concentration gradient verification shows that the linear relation of the kit is good, R2 is equal to 0.9915, clinical sample verification shows that the specificity of the kit is high, and the kit can be used for visual and portable detection of vibrio vulnificus.

The invention discloses an energy-saving pulping method based on pretreatment strengthening and segmented enzyme activation, and belongs to the technical field of pulping and papermaking. According to the pulping method provided by the invention, p-toluenesulfonic acid is added in pretreatment to adjust pH and strengthen enzyme activity, and enzyme is wrapped by using a temperature or pH response type carrier, so that pectinase, cellulase and laccase can be controlled to be released and activated in a proper treatment stage. By accurately controlling the enzyme action sequence, substrate competition is avoided, enzyme inactivation is reduced, the enzymolysis efficiency and the utilization rate are effectively improved, and the COD content of wastewater is reduced; and two-stage pulping and p-toluenesulfonic acid are combined to soften fibers synergistically, so that the power consumption per ton of pulp is reduced. According to the invention, fiber fibrillation is promoted through enzyme treatment, and the tensile strength and the water retention value are effectively improved; the laccase catalyzes crosslinking of lignin, so that the wet strength is remarkably improved. The paper pulp provided by the invention gives consideration to physical properties and environmental protection requirements of high-strength packaging paper manufacturing, and has a wide application prospect.

The present invention provides a novel means for degrading PEF. Provided is a method for degrading polyethylene furanoate that includes causing an enzyme comprising a polypeptide which has an amino acid sequence having at least 90% identity with the amino acid sequence of SEQ ID NO: 1 and has one or more amino acid substitutions selected from the group consisting of H129W, F208S, A172C, and A209C to act on polyethylene furanoate.

Provided is a vegetable milk having a reduced pink feeling and a changed taste. The present technology provides: an enzyme agent for reducing the pink feeling of a plant food or beverage or a plant food or beverage material, which contains a cell wall polysaccharide-degrading enzyme; and a plant food or beverage or a plant food or beverage material, which uses the enzyme agent for reducing the pink feeling. Also provided are: a method for producing a plant food or beverage or a plant food or beverage material, said method comprising a step for causing a cell wall polysaccharide-decomposing enzyme to act on a plant raw material; and a method for reducing the pink feeling of a plant food or beverage or beverage material.

The present invention relates to the field of bioengineering, and particularly to mutant compositions, fusion enzymes and methods for preparing collagen tripeptides. The present invention provides mutant compositions, including Mutant 1 and Mutant 2; Mutant 1 has an amino acid sequence as shown in SEQ ID NO: 1 and Mutant 2 has an amino acid sequence as shown in SEQ ID NO: 2. The preparation method provided by the present invention uses inexpensive amino acids as raw materials, and is highly efficiently converted into corresponding dipeptide or tripeptide products under the action of adenosine triphosphate (ATP) and the corresponding ligase. Introducing an ATP recycling system into the reaction system can greatly reduce its usage amount; at the same time, the amount of enzyme used in the actual production process is reduced by constructing fusion enzymes; the reaction and purification processes are further optimized by using immobilized enzyme catalysis, and the preparation scheme is very concise, with high yield, good product quality and easy to scale up.

[Problems] To provide plant-base milk with reduced powdery feel and altered taste.[Solution] The present technique provides an enzyme agent for reducing the powdery feel of a plant-base beverage or food or a plant-base beverage or food material, which contains a cell wall polysaccharide degrading enzyme, and a plant-base beverage or food or a plant-base beverage or food material using the enzyme agent for reducing powdery feel. The present technique also provides a method for producing a plant-base beverage or food or a plant-base beverage or food material, and a method for reducing the powdery feel of a plant-base beverage or food or a plant-base beverage or food material, which include a step of allowing a cell wall polysaccharide degrading enzyme to act on a plant-base raw material.

The invention discloses a preparation method of a proteoglycan protein conjugate carrying a specified connection site. The method comprises the following steps: carrying out recombinant expression on a recombinant tetanus toxin heavy chain C fragment rTTHc containing a Sortase A 7M ligase recognition sequence, introducing O, O '-(ethane-1, 2-diyl) bis (hydroxylamine) as a nucleophilic reagent into the C tail end of the rTTHc under the action of ligase Sortase A 7M, and carrying out specific condensation reaction on the C tail end of the rTTHc as a unique chemical reaction site and polysaccharide carrying an aldehyde group functional group to obtain the tetanus toxin heavy chain C fragment rTTHc. Therefore, polysaccharide protein conjugates with definite polysaccharide and protein connection sites are produced. According to the invention, the problem that the key antigen epitope of the carrier protein and the heterogeneity of the product are possibly damaged by the traditional random modification strategy is solved, the antigen epitope of the carrier protein is prevented from being damaged to a great extent, and a reliable technical scheme is provided for preparing the polysaccharide conjugate vaccine with high quality and high immunogenicity.

The present invention provides a method for producing nicotinamide mononucleotide, which uses one enzyme to produce nicotinamide mononucleotide from a nucleoside monophosphate, pyrophosphate, and nicotinamide as raw materials. The method for producing nicotinamide mononucleotide according to the present invention includes at least the following steps 1) and 2): 1) A first step of allowing substantially one enzyme to act on a nucleoside monophosphate and pyrophosphate to generate phosphoribosyl diphosphate; 2) A second step of allowing substantially only the above-mentioned one enzyme to act on the phosphoribosyl diphosphate as the product of the first step and nicotinamide to generate nicotinamide mononucleotide.

The present invention relates to a single base editor using adenosine deaminase acting on RNA (ADAR enzyme) and a single base editing method using same. Specifically, the present invention relates to a single base editor using an ADAR enzyme and a single base editing method using same, wherein the single base editor solves the problem of not only a target single base but also all single bases around the target single base being replaced, which is the biggest limitation of conventional base editing tools, and can selectively replace just a single base.

The present invention relates to a gene delivery platform for multi-organ sequential targeting oral administration, and to a gene delivery platform having a layer-by-layer chylomicron-mimicking self-assembly (lbl-CMSA), which is a lipid nanoparticle having a double-layer structure. A gene delivery platform for oral administration according to the present invention allows a second layer containing the outermost bile acid to exhibit high durability in the gastrointestinal tract even under various pH and enzyme actions, allows a gene drug encapsulated by an intestinal active target mechanism of bile acid to be primarily delivered to intestinal cells, and allows a first layer containing apolipoprotein to be delivered to lymphatic vessels through a chylomicron-mimicking pathway, thereby allowing the gene drug encapsulated therein to secondarily reach a target organ via a circulatory pathway in the body. Therefore, the gene delivery platform for oral administration of the present invention can be used as an effective oral administration formulation having increased bioavailability by loading, on the platform, a combination of gene therapeutic agents sequentially targeting the intestine and various organs.

To provide a method for efficiently and accurately distinguishing two similar diseases that differ in the presence or absence of a mutant allele in the same gene region.SOLUTION: A method for distinguishing between a genetic disease caused by a codon mutation in a gene and another disease includes the steps of: performing an amplification reaction to amplify a genetic region containing the codon, the amplification reaction including adding a DNA primer for amplifying the genetic region containing the codon and a PNA that inhibits the amplification of a normal allele containing the codon; and treating a product obtained by the amplification reaction with a restriction enzyme that specifically recognizes and cleaves either a base sequence containing the codon with the mutation or a base sequence containing the codon without the mutation, thereby obtaining a restriction enzyme-treated product.SELECTED DRAWING: Figure 3

The purpose of the present invention is to provide a processing technique for improving the liquid retention of a dried composition containing a vegetable protein. A method for improving the liquid retention of a dry composition containing a plant protein, said method comprising: an enzyme treatment step for obtaining a processed composition containing a plant protein by causing a protein deamidase to act on the composition containing a plant protein; and a drying step for drying the processed composition containing the vegetable protein to obtain a processed dried composition containing the vegetable protein, the method for improving the liquid retention property of the dried composition containing the vegetable protein, and the method for improving the liquid retention property of the dried composition containing the vegetable protein. The liquid retention property of the processed dry composition containing the vegetable protein when coexisting with a liquid can be improved.

The purpose of the present invention is to provide a processing technique for improving the foamability of a liquid composition containing oat protein. The foamability of a liquid composition containing oat protein can be improved according to a method for producing a processed liquid composition containing oat protein, said method comprising: an enzyme treatment step for obtaining an enzymatically treated liquid composition by causing a protein deamidase to act on the liquid composition containing oat protein at a prescribed pH; and a pH reduction step for reducing the pH of the enzyme-treated liquid composition to obtain a processed liquid composition containing oat protein.

This invention relates to the field of biodetection technology, specifically to a non-isolated digital multiplex detection method based on a DNA walker and TdT enzyme amplification. The method first utilizes coding microspheres with capture elements coupled to their surface to specifically bind to target miRNAs or proteins in the sample; then, specific guide DNA (gDNA) corresponding to the target molecule is released through enzymatic digestion or thermal denaturation; the gDNA then... Pf Under the action of Ago endonuclease, the DNA walker on the surface of the encoded microspheres is activated, cyclically cutting the orbital strand to generate multiple DNA fragments with 3'-hydroxyl ends. Finally, TdT enzyme is used to extend fluorescently labeled nucleotides at the hydroxyl ends, achieving in-situ signal amplification, and digital quantitative analysis is performed on the fluorescence signal of the encoded microspheres. This invention achieves high-sensitivity detection of multiple targets in a single reaction without chamber isolation, with detection limits for each target molecule reaching the fM or fg / mL level, providing an effective technical means for the early screening of major diseases such as Alzheimer's.

The invention belongs to the technical field of bioactive protein peptides / protein hydrolysates, and particularly relates to an anti-aging soybean protein peptide and a preparation method thereof. Soybean protein is used as a raw material, the soybean protein is firstly subjected to thermal denaturation treatment to change the secondary structure of the soybean protein, and then the alkaline subunit amount of the thermal denaturation soybean protein is increased by utilizing high static pressure physical treatment, so that the exposure of hydrophobic groups in soybean protein molecules and the increase of enzyme action sites are facilitated, and the proteolysis rate is further increased. And finally, carrying out enzymolysis on the treated soybean protein by using alkaline protease to obtain the soybean protein peptide. The soybean protein peptide obtained by the preparation method disclosed by the invention has very strong antioxidant activity and a good anti-aging effect.

Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

To provide a technique for reducing a saccharide in a plant-base food or beverage.The technique provides an enzymatic agent for reducing a saccharide in a plant-base food or beverage, which contains a cyclodextrin-producing enzyme, and a plant-base food or beverage using the enzymatic agent for reducing a saccharide. The technique also provides a method for manufacturing a plant-base food or beverage, which includes a step of allowing a cyclodextrin-producing enzyme to act in a liquefaction process of a plant-base raw material, and a method for reducing a saccharide in a plant-base food or beverage, which includes a step of allowing a cyclodextrin-producing enzyme to act on a plant-base raw material.

A simpler method for measuring enzyme activity is provided. The-method for estimating enzyme activity uses a base on which spots of immobilized substrates are formed, and includes (a) enabling an enzyme to act on the substrate on the spot to obtain an enzyme product; (b) setting one or more compartments on the spot and obtaining density of the substrate in each compartment; (c) measuring the density of the enzyme product in the each compartment; and (d) estimating the enzyme activity of the enzyme based on a relationship between the density of the substrate in the each compartment obtained by step (b) and the density of the enzyme product in the each compartment obtained by step (c).

This invention discloses a method for synthesizing chiral γ-acyloxybutenoic acid lactones, characterized by the following steps: (1) dissolving the substrate 2-furoic acid in an organic solvent and carrying out a photocatalytic oxidation reaction under the action of a photocatalyst and under light and oxygen conditions; (2) after the substrate in step (1) is completely converted, adding an acyl donor, and carrying out an enzymatic acylation reaction in the resulting reaction solution under the action of lipase to obtain γ-acyloxybutenoic acid lactones. This invention solves the problem of solvent compatibility between photocatalysis and enzyme catalysis systems, supporting both efficient photocatalytic oxidation and excellent medium for enzyme catalytic acylation. It realizes a one-pot conversion from the substrate 2-furoic acid to the product chiral γ-acyloxybutenoic acid lactones, with advantages such as mild reaction conditions, environmental friendliness, high synthesis efficiency, excellent selectivity, easy scale-up, and no need for separation and purification of intermediate products.

The invention relates to the technical field of extract refining, and particularly discloses a method for preparing stevioside RM. The method comprises the following steps: (1) crushing dry stevia rebaudiana leaves, mixing with water, extracting, stirring, extracting, centrifugally filtering, performing column chromatography purification, washing with water, eluting with ethanol to obtain an eluent, and concentrating the eluent to obtain a stevioside solution; (2) adding an auxiliary stable mixed aid, cane sugar, cane sugar synthase, beta-1, 2-UDP-glucosyltransferase from dendrobenthamia grandiflora and beta-1, 3-UDP-glucosyltransferase from walnut into the stevioside solution obtained in the step (1), carrying out decoloration and desalination treatment after catalytic reaction, eluting with an ethanol-water solution, and carrying out vacuum drying to obtain the stevioside. And finally, carrying out vacuum concentration, recrystallization and drying treatment to obtain the product. According to the method, the stability of an enzyme action system in the stevioside RM preparation process can be remarkably improved.

To provide a technique for improving the shape-retainability of a plant-base ice cream.The technique provides an enzyme preparation for improving the shape-retainability of a plant-base ice cream, which contains one or more enzymes selected from the group consisting of maltotriose-producing amylase, lipase, and β-amylase, and a plant-base ice cream using the enzyme preparation for improving the shape-retainability. The technique also provides a method for producing a plant-base ice cream and a method for improving the shape-retainability of a plant-base ice cream, which include one or more steps selected from a maltotriose-producing amylase action step of allowing maltotriose-producing amylase to act on a carbohydrate-containing plant-base material, a lipase action step of allowing lipase to act on a fat-containing plant-base material, and a β-amylase action step of allowing β-amylase to act.

The invention relates to the technical field of deep processing of edible mushrooms, and discloses a method for improving the flavor of an edible mushroom product and application. The method comprises the following steps: introducing a complex enzyme dissolving solution containing papain and 5 '-phosphodiesterase in a ratio of 1: 1 into an edible mushroom tissue by combining ultrasonic treatment with a pulse negative pressure permeation technology, performing enzymolysis, and performing high-temperature sterilization and enzyme deactivation. The ultrasonic hole effect and the mechanical effect are innovatively utilized to break through an enzyme action channel, pulse negative pressure promotes enzyme liquid permeation, and Ca < + > is cooperated to enhance the enzyme activity and texture stability. The content of glutamic acid in the treated edible mushroom product is increased by 25%, the total amount of 5 '-nucleotide is increased by 3.34 times, the equivalent delicate flavor concentration reaches 4.75 g / 100g, and the sensory score is remarkably improved. The method is suitable for processing multiple edible mushroom slices / granules such as shiitake mushrooms and pleurotus eryngii, the problem that the flavor of an original product is boring is effectively solved, the original state is kept while green processing is achieved, and a new scheme is provided for flavor upgrading of leisure food and prefabricated dishes.

The invention provides a synthesis method of a single-chain aptamer and application of the single-chain aptamer in synthesis of a C5 complement inhibitor, and relates to the technical field of aptamer synthesis, the synthesis method comprises the following steps: cutting off the single-chain aptamer into two fragments, pairing the two fragments through complementary bases carried by the two fragments to form a double-chain secondary structure with a single gap, and under the enzymatic action, synthesizing the C5 complement inhibitor by using the double-chain secondary structure to obtain the C5 complement inhibitor. A phosphodiester bond is formed at a notch of the double-chain secondary structure to obtain a single-chain aptamer, and the base length ratio of the two fragments is (14-26): (25-13). The self-complementary nucleic acid fragment of the to-be-synthesized single-stranded aptamer is used as a substrate, specific connection is realized under the action of ligase, an exogenous template chain is not needed, and the final purity and yield of the single-stranded aptamer are improved. The technical problems that in the prior art, enzymatic connection of single-stranded oligonucleotides needs to depend on an exogenous template, so that an experimental system is complex, mismatching is likely to happen, and connection position errors are likely to happen are solved.

Provided is a novel technique for improving the freezing resistance of a starch-containing food. Provided is a method for producing a starch-containing composition for freezing, the method comprising an enzyme action step for causing one or more enzymes selected from the group consisting of a microorganism-derived beta-amylase, a soybean-derived beta-amylase, and a 4-alpha-glucanotransferase to act on a starch-containing material. The invention also provides a preparation method of the starch-containing composition, which comprises a freezing process, and a freezing step for freezing an enzyme-treated starch-containing composition obtained by causing one or more enzymes selected from the group consisting of a microorganism-derived beta-amylase, a soybean-derived beta-amylase, and a 4-alpha-glucanotransferase to act on the starch-containing material.

The application discloses a synthesis method and application of a fluorescent probe for lipase detection, and the fluorescent probe has the following structural formula; the synthesis method comprises the following steps: under the action of inert gas protection, a catalyst and alkali, triphenylol compound and a fatty chain or an aromatic ring organic acid are reacted in an organic solvent at 25-100 DEG C for 8-24h; after the reaction is stopped, dichloromethane and water are added to carry out extraction; organic phases are combined, dried, filtered, and the solvent is removed under reduced pressure; and the product is separated and purified; the fluorescent probe is basically non-fluorescent by itself, releases bright fluorescence after reacting with lipase, and can realize rapid detection of bacteria by using the characteristics that most bacteria secrete lipase in the growth and reproduction process.

The invention discloses a glycosyl transferase gene for biosynthesis of polygonatum cyrtonema steroid saponin and application of the glycosyl transferase gene. The glycosyl transferase gene directly related to steroid saponin synthesis in the UGT85A subfamily is cloned and functionally verified from polygonatum cyrtonema for the first time, and gene resources of steroid saponin biosynthesis are enriched; the activity of the PcUGT85As gene for catalyzing the glycosylation of the sitenogenin is defined, and the key enzyme action of the PcUGT85As gene is proved; a key target spot is provided for realizing metabolic regulation and synthetic biological production of the polygonatum cyrtonema active saponin; the gene can be applied to microbial cell factory construction, transgenic plant breeding or biological manufacturing of high-yield saponin, and has a good application prospect and industrial value.