Alpha-L-fucosidase detection kit and preparation method thereof

A kit and reagent technology are applied in the field of α-L-fucosidase content detection in serum samples to achieve the effects of strong anti-interference, good stability and good repeatability

Active Publication Date: 2016-01-13
WUHAN LIFE ORIGIN BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] With the continuous improvement and improvement of AFU detection methodology, some diagnostic kits suitable for AFU detection have appeared, but these detection kits will have some detec

Method used

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  • Alpha-L-fucosidase detection kit and preparation method thereof
  • Alpha-L-fucosidase detection kit and preparation method thereof
  • Alpha-L-fucosidase detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of Example 1 α-L-fucosidase Detection Kit:

[0046] 1. Preparation of Reagent 1:

[0047] Take each component according to the stated dosage:

[0048]

[0049] Dissolve each component in distilled water or double-distilled water, mix well, adjust the pH to 6.5-8.5 with 1mol / L hydrochloric acid or sodium hydroxide solution, and then adjust the volume to 1L with distilled water or double-distilled water.

[0050] 2. Preparation of reagent 2:

[0051] Take each component according to the stated dosage:

[0052]

[0053] Dissolve each component in distilled water or double-distilled water, mix well, adjust the pH to 6.5-8.5 with 1mol / L hydrochloric acid or sodium hydroxide solution, and then adjust the volume to 1L with distilled water or double-distilled water.

Embodiment 2

[0054] Preparation of Example 2 α-L-fucosidase Detection Kit:

[0055] 1. Preparation of Reagent 1:

[0056] Take each component according to the stated dosage:

[0057]

[0058] Dissolve each component in distilled water or double-distilled water, mix well, adjust the pH to 6.5-8.5 with 1mol / L hydrochloric acid or sodium hydroxide solution, and then adjust the volume to 1L with distilled water or double-distilled water.

[0059] 2. Preparation of reagent 2:

[0060] Take each component according to the stated dosage:

[0061]

[0062] Dissolve each component in distilled water or double-distilled water, mix well, adjust the pH to 6.5-8.5 with 1mol / L hydrochloric acid or sodium hydroxide solution, and then adjust the volume to 1L with distilled water or double-distilled water.

Embodiment 3

[0063] Embodiment 3 Preparation of α-L-fucosidase detection kit:

[0064] 1. Preparation of Reagent 1:

[0065] Take each component according to the stated dosage:

[0066]

[0067] Dissolve each component in distilled water or double-distilled water, mix well, adjust the pH to 6.5-8.5 with 1mol / L hydrochloric acid or sodium hydroxide solution, and then adjust the volume to 1L with distilled water or double-distilled water.

[0068] 2. Preparation of reagent 2:

[0069] Take each component according to the stated dosage:

[0070]

[0071] Dissolve each component in distilled water or double-distilled water, mix well, adjust the pH to 6.5-8.5 with 1mol / L hydrochloric acid or sodium hydroxide solution, and then adjust the volume to 1L with distilled water or double-distilled water.

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Abstract

The invention discloses an alpha-L-fucosidase detection kit consists of an independent reagent 1 and an independent reagent 2, wherein the reagent 1 consists of components of a buffer agent, a preservative, a metal ion chelating agent, a stabilizing agent and water; and the reagent 2 consists of components of a buffer agent, a preservative, an enzyme action substrate, a surfactant, a stabilizing agent and water. The preparation method comprises the following steps: (1) preparing the reagent 1: dissolving the various components in distilled water or double distilled water, uniformly mixing, regulating pH to 6.5-8.5, and setting a constant volume; (2) preparing the reagent 2: dissolving various components in distilled water or double distilled water, uniformly mixing, regulating pH to 6.5-8.5, and setting a constant volume; and (3) independently sub-packaging the reagent 1 and the reagent 2, and sealing. The detection kit disclosed by the invention has the advantages of good stability, high precision, broad linear test range, good repeatability, strong anti-interference performance and the like.

Description

technical field [0001] The invention relates to a reagent for detecting α-L-fucosidase in human serum and a preparation method thereof, belonging to the technical field of detecting the content of α-L-fucosidase in serum samples. Background technique [0002] α-L-Fucosidase (α-L-Fucosidase, AFU) is a lysosomal acid hydrolase, which is widely distributed in various tissues, cells and body fluids in the human body. In 1977, Bauer et al. observed in animal experiments that the AFU activity in Morris rat liver cancer tissue was 7 times higher than that in normal liver, and it was related to the growth period of the tumor. In 1980, French scholar Dcugnier et al. first found elevated AFU activity in the serum of 3 cases of primary liver cancer, and proposed AFU as a new marker enzyme for the diagnosis of liver cancer, which was confirmed by many subsequent studies. In recent years, research on the methodology of AFU detection and the changes of serum AFU levels in other tumor or ...

Claims

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Application Information

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IPC IPC(8): C12Q1/34
Inventor 华权高来祥兵许可
Owner WUHAN LIFE ORIGIN BIOTECH LTD
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