Hesperetin dihydrochalcone-7-O-glucoside and preparation method and application thereof

A technology for hesperetin dihydrochalcone and glucoside, which is applied in the field of hesperetin dihydrochalcone-7-O-glucoside and its preparation, can solve problems such as limited effect, and achieves the purpose of expanding the scope of use. Effect

Inactive Publication Date: 2010-07-28
GUANGDONG FOOD IND INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This taste correcting method, due to its lim...

Method used

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  • Hesperetin dihydrochalcone-7-O-glucoside and preparation method and application thereof
  • Hesperetin dihydrochalcone-7-O-glucoside and preparation method and application thereof
  • Hesperetin dihydrochalcone-7-O-glucoside and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] 2.0 g of citric acid was dissolved in 80 ml of distilled water, and its pH value was adjusted to 6.0 with 30% NaOH solution. Add 1g of neohesperidin dihydrochalcone and 0.01g of naringinase (enzyme activity 860u / g) to the above mixed solution, stir and react at 40°C for 24h, TLC tracking shows that neohesperidin dihydrochalcone The ketone moiety is converted to hesperetin dihydrochalcone-7-O-glucoside. After the reaction, heat the reaction mixture to 80°C to inactivate the enzyme, concentrate the solution, pass through the AB-8 macroporous resin column, wash the column with distilled water to remove sugar and inorganic salts, and then wash the column with 40% ethanol solution , concentrated and spin-dried to obtain 0.9 g of the product, namely hesperetin dihydrochalcone-7-O-glucoside.

Embodiment 2

[0033] Dissolve 2.0 g of citric acid in 80 ml of distilled water, adjust its pH value to 6.6 with 30% NaOH solution, then add 24 ml of ethyl acetate solution and stir to mix. Add 1g neohesperidin dihydrochalcone and 0.01g naringinase (enzyme activity 860u / g) to the above mixed solution, stir and react at 40°C for 30h, TLC tracking shows that neohesperidin dihydrochalcone Ketones are mostly converted to hesperetin dihydrochalcone-7-O-glucoside. After the reaction is over, heat the reaction mixture to 80°C to inactivate the enzyme, concentrate the solution, pass through the AB-8 macroporous resin column, wash the column with distilled water to remove sugar and inorganic salts, and then wash the column with 30% ethanol solution , concentrated and spin-dried to obtain 1.3 g of the product, namely hesperetin dihydrochalcone-7-O-glucoside.

Embodiment 3

[0035] Dissolve 2.0 g of citric acid in 80 ml of distilled water, adjust its pH value to 7.0 with 15% KOH solution, then add 20 ml of ethyl acetate solution and stir to mix. Add 1g of neohesperidin dihydrochalcone and 0.02g of naringinase (enzyme activity 860u / g) to the above mixed solution, stir and react at 35°C for 24h, TLC tracking shows that neohesperidin dihydrochalcone Ketones are mostly converted to hesperetin dihydrochalcone-7-O-glucoside. After the reaction, heat the reaction mixture to 80°C to inactivate the enzyme, concentrate the solution, pass through the AB-8 macroporous resin column, wash the column with distilled water to remove sugar and inorganic salts, and then wash the column with 40% ethanol solution , concentrated and spin-dried to obtain 1.7 g of the product, which was decolorized by activated carbon and crystallized from a mixed solvent of ethanol / water to obtain 1.1 g of white crystal hesperetin dihydrochalcone-7-O-glucoside, the content of which was ...

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Abstract

The invention discloses a hesperetin dihydrochalcone-7-O-glucoside, wherein the compound is obtained by generating a rhamnose deglycosylation reaction of new hesperidin dihydrochalcone in water or a water and organic solvent system under an enzyme action. The sweetness multiple of the hesperetin dihydrochalcone-7-O-glucoside prepared by the invention is about 1800 times of sucrose, and the hesperetin dihydrochalcone-7-O-glucoside can be widely used for industries of foods, medicines, feed, daily use chemicals and the like and used as a sweetening agent, a flavoring agent, a flavor enhancer and the like.

Description

technical field [0001] The invention relates to a food additive and its preparation method and application, in particular to hesperetin dihydrochalcone-7-O-glucoside and its preparation method and application. Background technique [0002] Food high-intensity sweeteners refer to sweeteners whose sweetness is more than several dozen times that of sucrose, and are divided into two categories: natural and chemically synthesized. Natural high-intensity sweeteners are popular in the market due to their good safety and easy acceptance by consumers. At present, natural high-intensity sweeteners mainly include stevioside, glycyrrhizin, mogroside, dihydrochalcone and thaumatin. [0003] Dihydrochalcone is a sweet compound originally isolated from citrus and naringin. People have synthesized a large number of derivatives of this type of sweetener, some of which have only made some slight changes in structure, and some have made major changes. All of these are to further understand ...

Claims

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Application Information

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IPC IPC(8): C07H15/203C12P19/44A23L1/236A23L1/226A61K47/26A23L27/20A23L27/30
Inventor 王三永李春荣王辉臧国雄
Owner GUANGDONG FOOD IND INST
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