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A banana fruit glycogen initial synthetase gene magn12 and its encoded protein and application

A technology for synthesizing enzymes and fruits, which is applied in the fields of application, genetic engineering, and plant genetic improvement, etc., can solve problems such as GN reports that have not been seen, and achieve the effect of improving the quality of sweetness and increasing the content of fructose

Active Publication Date: 2020-05-12
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, most studies on GN mainly focus on animals (Bischof et al., 2013; Li et al., 2017), and there is little information on GN in plants, especially for the important tropical fruit banana, where GN has not been seen so far related reports

Method used

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  • A banana fruit glycogen initial synthetase gene magn12 and its encoded protein and application
  • A banana fruit glycogen initial synthetase gene magn12 and its encoded protein and application
  • A banana fruit glycogen initial synthetase gene magn12 and its encoded protein and application

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Embodiment Construction

[0020] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention.

[0021] 1. Gene acquisition

[0022] Using the cDNA of banana fruit as a template, the

[0023] 5'-GCTCTAGAGATGATGTACATGGGGAC-3'

[0024] 5'-CGAGCTCGGTTAATACAGTTTGTTGAACTCAG-3'

[0025] As a primer, a base sequence obtained by PCR amplification is 1407bp ( figure 1 ), its sequence is shown in SEQ ID No:1; the amino acid sequence of the gene encoding MaGN12 is shown in SEQ ID No:2.

[0026] 2. Expression vector construction

[0027] The nucleotide sequence of the above-mentioned banana fruit glycogen synthetase gene MaGN12 was double digested with Xba I and Sac I two restriction endonucleases respectively, and the target fragment after digestion was The plant expression vector pBI121 fragment was recovered, connected, transformed and sequenced to verify that it was correct, and the expres...

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Abstract

The invention provides a banana fruit glycogen initiation synthetase gene MaGN12. The nucleotide sequence is shown in SEQ ID NO:1. The invention also provides an encoded protein and application of thebanana fruit glycogen initiation synthetase gene MaGN12. The banana fruit glycogen initiation synthetase gene MaGN12 can improve the sweetness quality of the fruit and increase the glucose and fructose content of the fruit. For example, the gene can be imported into tomatoes to obtain 8 independent lines of MaGN12 transgenic tomatoes driven by a 35S promoter. The over-expression gene MaGN12 increases GN enzyme activity, MaGN12 expression, glycogen content, fructose and glucose content, which is important for regulating fruit quality and cultivating high quality banana varieties.

Description

technical field [0001] In the field of biological technology, the invention specifically relates to a banana fruit glycogen initial synthetase gene MaGN12 and its encoded protein and application. Background technique [0002] Glycogen is a branched macromolecular polysaccharide composed of glucose, which is widely stored in human body, animals, fungi, bacteria, and plant tissues. The lack of glycogen in human heart and skeletal muscle leads to cardiomyopathy and muscle weakness; animal glycogen mainly controls the homeostasis of blood sugar; glycogen in fungi and bacteria mainly exists in the form of basic energy, which can be shortened when needed. Use quickly and in large quantities within a short period of time, and quickly restore storage when not needed. Phytoglycogen is a kind of soluble α-D-glucan existing in plants lacking isoamylase mutants (sul mutants). It is the main storage polysaccharide in plants and the basis of the carbon framework of all plant organs. sub...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/55C12N15/54C12N9/10C12N9/16C12N15/82A01H5/00A01H6/82C12N15/11
Inventor 金志强苗红霞徐碧玉孙佩光刘菊华贾彩红张建斌王静毅王卓苗雨露
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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