A method and application of using β-mannanase to prepare low-viscosity octenyl succinate konjac glucomannan ester

Abstract

The invention relates to a method and application for preparing low-viscosity octenyl succinate konjac glucomannan ester by using β-mannanase. The present invention creatively uses natural non-toxic, non-polluting β-mannanase as raw material, uses it to enzymatically degrade konjac glucomannan, and then further esterifies and modifies the polysaccharide to obtain the β-mannanase of the present invention. Viscosity target product. The target product octenyl succinic acid konjac glucomannan ester obtained by the present invention has low viscosity, and the viscosity under 1wt% concentration condition can be as low as 2700mPa.s (25 ℃), therefore, can be used as an emulsifier to emulsify corn germ oil , broaden the scope of application of traditional konjac glucomannan ester. In addition, the present invention also applies microwave heating to the modification of KGM, which can achieve the effects of high efficiency, energy saving, emission reduction and consumption reduction. Moreover, compared with the solvent method of the traditional reaction, the reaction system of the present invention is in a semi-dry state, and there is no waste water discharge in the whole reaction process, which has a significant environmental protection effect.

Description

technical field

[0001] The invention relates to the fields of food processing, biology, medicine, chemical industry and the like, in particular to a method and application for preparing low-viscosity octenyl succinate konjac glucomannan ester by using β-mannanase. Background technique

[0002] Konjac Glucomannan (KGM for short) is formed by polymerizing glucose and mannose residues with a molecular ratio of 1:1.5~1:1.78 through β-1,4 glycosidic bonds and β-1,3 glycosidic bonds The water-soluble polysaccharide of the sugar main chain has a β-1,3 bond branch at the C-3 position of the mannosyl group, and an acetyl group is connected to every 19 sugar residues (C-6 position). Natural KGM is composed of radially arranged micelles, and its crystal structure has two types: α-type (amorphous) and β-type (crystalline). KGM has a relatively high molecular weight, mainly distributed between 200,000 and 2 million, and the weight average molecular weight Mw is 5.83×10 5 g / mol, its mol...