Method for producing galacto-mannan-oligosaccharides by enzymatic degradation of sophora bean gum

A technology of galactomannan oligosaccharides and enzymatic degradation, applied in the field of enzymatic degradation of locust bean gum to produce galactomannan oligosaccharides, can solve problems such as the method of producing galactomannan oligosaccharides from locust bean gum that has not been seen, and achieve the product The effect of good structural stability, low cost and mild preparation conditions

Inactive Publication Date: 2009-04-29
OCEAN UNIV OF CHINA
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There is no report on the method of enzymatically degrading locust bean gum to produce galactomannan oligosaccharides

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  • Method for producing galacto-mannan-oligosaccharides by enzymatic degradation of sophora bean gum

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Embodiment Construction

[0010] The bacterial species alkalophilic bacillus used in the present invention can be cultivated on common bacterial culture mediums such as nutrient broth or beef extract peptone medium, and after liquid shaking culture for 18-24 hours, the growth reaches the logarithmic phase, and the culture solution at this time can be used as Fermented seeds are used for inoculation of enzyme-producing medium. Prepare the enzyme-producing medium according to the following components: 4 g of guar gum, 20 g of soybean powder, (NH 4 ) 2 HPO 4 5g,NH 4 Cl 1g, K 2 HPO 4 ·3H 2 O 1g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.5g, CaCl 2 0.1g, FeSO 4 ·7H 2 O 0.001g, finally add water to a total weight of 1kg, adjust the pH of the enzyme culture medium to 8.0 with 1M NaOH or HCl aqueous solution. After the enzyme-producing medium was sterilized by high-pressure steam at 121°C for 20 minutes, it was cooled to room temperature, and an alkalophilic bacillus culture solution in the logarithmic phase wa...

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Abstract

The invention provides a method for producing galacto-mannan-oligosaccharide by the enzymic degradation of locust bean gum. The method is characterized in that: preparing enzyme-producing culture medium, inoculating bacillus alcalophilus, fermenting, extracting coarse enzyme liquid of mannase, dissolving the locust bean gum in buffer solution, adding the coarse enzyme liquid of the mannase into the solution for enzymatic degradation, inactivating the mannase by boiling water bath heating, removing deposited impurities after centrifuging, making the precipitation fractionation by organic solvent after concentration, and obtaining the galacto-mannan-oligosaccharide fragment after freeze drying. The preparation method has the advantages of low requirement on environmental equipment, simple and easy operation, low cost, simple composition of the product, mild preparation conditions, good structural stability of the product and suitability for popularization.

Description

technical field [0001] The invention relates to a method for enzymatically degrading locust bean gum to produce galactomannan oligosaccharides. Background technique [0002] Locust bean gum is a galactomannan with mannose as the main chain. The average molar ratio of galactose to mannose is 1:4. It can be used as a food thickener, emulsifier, stabilizer, etc. The mannan oligosaccharide formed after mannan degradation is used as the proliferation factor of bifidobacteria, which can effectively make the beneficial bacteria in the human body proliferate naturally, improve the structure of intestinal flora, and regulate intestinal function. Therefore, mannan oligosaccharide has the function of protecting the liver , anti-tumor, enhance immunity, enhance intestinal peristalsis, lower cholesterol, anti-aging and other physiological activities, suitable for various intestinal diseases, but also suitable for healthy people, especially the elderly and children's daily health care, ha...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12R1/07
Inventor 牟海津江晓路王鹏周芳
Owner OCEAN UNIV OF CHINA
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