A kind of Arthrobacter aureus and its application in the preparation of okra polysaccharide degrading enzyme

A polysaccharide-degrading enzyme, chromobacter chromobacter technology, applied in the direction of enzymes, bacteria, microorganism-based methods, etc., can solve the problem of undiscovered okra polysaccharide-degrading enzymes, etc.

Active Publication Date: 2020-07-07
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about okra polysaccharide degrading enzymes at home and abroad

Method used

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  • A kind of Arthrobacter aureus and its application in the preparation of okra polysaccharide degrading enzyme
  • A kind of Arthrobacter aureus and its application in the preparation of okra polysaccharide degrading enzyme
  • A kind of Arthrobacter aureus and its application in the preparation of okra polysaccharide degrading enzyme

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Morphological, physiological and biochemical characteristics and molecular biology identification of embodiment 1 Arthrobacter aurescens (Arthrobacter aurescens) TU

[0045] A strain of Arthrobacter aurescens was isolated from okra planting soil in Qingzhou, Weifang by using okra polysaccharide medium, named Arthrobacter aurescens TU, and the strain of Arthrobacter aurescens TU was preserved, strain preservation information As follows: deposit number: CCTCC NO: M 2019035; depository unit: China Center for Type Culture Collection; deposit address: Wuhan University, Wuhan, China; deposit date: January 11, 2019.

[0046] The morphology, physiological and biochemical characteristics and molecular biological identification results of Arthrobacter aurescens TU are as follows:

[0047] 1. The morphological characteristics, physiological and biochemical characteristics of Arthrobacter aurescens TU are identified by conventional strain identification methods, and the specific re...

Embodiment 2

[0053] The growth characteristic analysis of embodiment 2 Arthrobacter aurescens (Arthrobacter aurescens) TU

[0054] (1) Preparation of seed solution: Inoculate Arthrobacter aureus TU stored on okra polysaccharide medium into fermentation medium, rotate at 150 r / min, and culture at 30° C. for 48 hours.

[0055] (2) The effect of temperature on the growth of the strain: the seed solution was inoculated in the fermentation medium with an inoculum size of 5%, the pH was 7.0, and the rotation speed was 150r / min, and cultured at different temperatures respectively, and the cell concentration was measured. The results showed that the bacteria could grow in the temperature range of 10-55℃, and the optimum growth temperature was 35℃.

[0056] (3) The influence of pH on bacterial strain growth: adopt 2M hydrochloric acid and 1 M sodium hydroxide to regulate the pH of culture medium after the sterilization of fermentation medium, make the pH of the fermentation medium after sterilizati...

Embodiment 3

[0057] Embodiment 3 Arthrobacter aurescens (Arthrobacter aurescens) TU produces the method for okra polysaccharide degrading enzyme

[0058] The present embodiment provides a preparation method of okra polysaccharide degrading enzyme, which is prepared by fermentation of Arthrobacter aurescens TU, specifically as follows:

[0059] (1) Preparation of seed solution: Inoculate Arthrobacter aureus TU stored on the okra polysaccharide medium into the fermentation medium, rotate at 130r / min, and cultivate at 30°C for 24h to obtain the seed solution;

[0060] (2) Production of okra polysaccharide degrading enzyme by fermentation: inoculate the seed solution obtained in step (1) into the fermentation medium at an inoculum size of 6%, cultivate at 130r / min, and cultivate at 30°C for 48h;

[0061] (3) Extraction and purification of okra polysaccharide degrading enzyme: the fermentation broth obtained in step (2) is centrifuged at 10000r / min for 10min, and the supernatant is taken; 45% a...

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Abstract

The invention relates to the technical field of biology, in particular to arthrobacter aurescens and application of the arthrobacter aurescens in preparation of an okra polysaccharide degrading enzyme. The arthrobacter aurescens (TU) is preserved in China Center for Type Culture Collection on January eleventh, 2019, and the preservation number is CCTCC NO:M 2019035. The strain can efficiently secrete the okra polysaccharide degrading enzyme and can be applied to preparation of the okra polysaccharide degrading enzyme. The okra polysaccharide degrading enzyme generated by the strain has high okra polysaccharide degrading activity, high heat stability and wide temperature and pH adaption range, efficient resources are provided for okra polysaccharide degrading through an enzyme method, and an advantageous technical means is provided for high-value utilization of okra.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an Arthrobacter aureus and its application in preparing okra polysaccharide degrading enzymes. Background technique [0002] Okra (Abelmoschus esculent, also known as okra), an annual herb of the Malvaceae family, is a dual-purpose plant for medicine, flowers, vegetables and feed. Introduced from Taiwan and Japan in the 1990s, it is now planted in Shandong, Guangdong, Jiangsu, Zhejiang, Hainan and Fujian. In recent years, the planting area of ​​Shandong okra has been on the rise. At present, the planting area has reached more than 100,000 mu, accounting for about one-third of the planting area in China. At present, the sales of okra in Shandong Province mainly rely on orders from processing companies. 80% of okra is exported to Japan, Korea and other countries after quick-frozen processing as special dishes, and a small part of okra is supplied to the domestic market as fresh vegeta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/00C12R1/06
Inventor 李银平王新宇洪自信刘艳玲王锡爽闫鸣艳
Owner QINGDAO UNIV OF SCI & TECH
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