130 results about "Phenylethanoid" patented technology

The objective of the invention is to provide a photo-aging prophylactic agent for skin with novel ingredients and foods using the photo-aging prophylactic agent for skin, medicaments for mammals including human beings and a skin external agent, as well as feed for mammals. The inventive photo-aging prophylactic agent for skin is characterized by taking extracts of Orobanchaceae plants as effective ingredients. The photo-aging prophylactic agent for skin preferably comprises phenylethanoid glycosides compounds as effective ingredients. Moreover, the photo-aging prophylactic agent for skin preferably comprises at least one of echinacoside and acteoside as the phenylethanoid glycosides compounds. On top of that, the photo-aging prophylactic agent for skin preferably comprises both echinacoside and acteoside as the phenylethanoid glycosides compounds. In addition, the photo-aging prophylactic agent for skin can be employed as medicaments for mammals including human beings, skin external agents, consumptive foods and feed for mammals.

The body of a herbaceous plant, Cistanche tubulosa (Schenk.) Wight, is used to make a medicinal preparation containing phenylethanoid glycosides and comprising 10–70% of echinacoside by weight of the preparation and 1–40% of acteoside by weight of the preparation. The medicinal preparation is used as an active ingredient of medicinal composition for use in prevention of senile dementia, and inhibition of aggregation of blood platelets.

The invention relates to a preparation method of cistanche deserticola phenylethanoid glycosides. The method comprises the steps of: cistanche deserticola crushing, lixiviating, absorbent resin adsorption, elution, and concentration, etc. In research of the extraction and stability of cistanche deserticola phenylethanoid glycoside, under a weakly acidic condition with pH of 2-4, the cistanche deserticola phenylethanoid glycoside can have substantially improved stability, and under the condition, the cistanche deserticola phenylethanoid glycoside extracted from cistanche deserticola is mainly composed of echinacoside and verbascoside. And in cistanche deserticola phenylethanoid glycosides, the total content proportion of echinacoside and verbascoside is greater than 90%. The content of cistanche deserticola phenylethanoid glycosides extracted by the method of the invention is obviously higher when compared with other extraction methods. And the obtained cistanche deserticola extract has stable effective component content and relative light color. The method provided in the invention avoids decomposition and oxidation of cistanche deserticola phenylethanoid glycoside, and is in favor of product stability.

The invention discloses an osmanthus phenylethanoid glycoside extract. The total phenylethanoid glycoside content in the osmanthus phenylethanoid glycoside extract is 30-90 wt%; and the total phenylethanoid glycosides comprise verbascoside and isoverbascoside. The verbascoside content in the osmanthus phenylethanoid glycoside extract is 25-80 wt%, and the weight ratio of the verbascoside to the isoverbascoside in the total phenylethanoid glycosides is 48:1-300:1. The invention also discloses a preparation method of the osmanthus phenylethanoid glycoside extract and application of the osmanthus phenylethanoid glycoside extract in preparing foods, drugs or health products for preventing and treating diabetes.

The invention provides a method that effectively stops and restrains the active ingredients of desertliving cistanche from serious degrading or losing caused by enzyme catalytic reaction initiated during the processing, drying and production technology processes by adopting an improved drying processing manner of the desertliving cistanche. The method is characterized in that: microwave is used for heating and sterilizing enzyme at high temperature, polar molecules contained in the meat stalk cell of fresh desertliving cistanche that is rich in water are led to make high frequency swing, vibration and friction and extrusion between the molecules by using a microwave high frequency electric field so as to lead the temperature inside the cell of the desertliving cistanche and the surface thereof to raise rapidly, thus generating thermal effect that has the function of enzyme sterilization and decoction pieces of the desertliving cistanche is dried rapidly by adopting microwave drying technology, thus effectively preserving the content of the active ingredients of phenylethanoid glycosides, etc. that is rich in the meat stalk of the fresh desertliving cistanche and greatly promoting the quality and medical value of the prepared decoction pieces of the desertliving cistanche. Upon analysis and determination, the content of the active ingredients of the phenylethanoid glycosides, etc. contained in the prepared decoction pieces, which is in the shapes of filiform, elongated flake or granular, of the desertliving cistanche is rich, the content of the main index ingredients of echinacoside and verbascoside monomeric compounds of the prepared decoction pieces of the desertliving cistanche, which used for weighing the quality thereof, is up to 13-24.1 percent (being calculated based on dry substance) and the water content thereof is 1.2-3.8 percent.

The invention provides a method for extracting phenylethanoid glycoside in herba cistanche by using a salt-induced deep-eutectic solvent on the one hand. According to the method, the phenylethanoid glycoside in the herba cistanche is extracted by using a water solution of the deep-eutectic solvent to extract herba cistanche powder, and adding phosphate into extracting liquid to prepare an aqueous two-phase system. According to the method, conditions of the extraction environment are mild, and phase separation time is short; the yield of the phenylethanoid glycoside in the herba cistanche can reach up to 20%.

The invention relates to a method for simultaneously preparing phenylethanoid glycoside, mannitol, oligosaccharide syrup and polysaccharide from broomrape, which comprises the following steps: extracting monosaccharide, oligosaccharide and phenylethanoid glycoside in broomrape by using an ethanol water solution, separating the phenylethanoid glycoside and saccharide with a macroporous adsorbent resin column, carrying out concentration and drying on the phenylethanoid glycoside solution obtained by elution to obtain the product, concentrating the saccharide under reduced pressure, crystallizing at normal temperature, separating the oligosaccharide and mannitol, recrystallizing the mannitol to obtain the high-purity mannitol, centrifugating the clear solution to obtain the oligosaccharide syrup, extracting the polysaccharide from residues by using pure water, and carrying out alcohol precipitation to collect the polysaccharide. The method enhances the comprehensive utilization ratio of the broomrape resources, simplifies the production technique, develops the phenylethanoid glycoside, mannitol, oligosaccharide syrup and polysaccharide products simultaneously, increases the economic added value of the broomrape product, and has favorable industrial production and market development prospects.

The present invention relates to an extraction and separation method for echinacoside and verbascoside in cistanche. The method comprises the following steps: dicing the cistanche and drying the cistanche; adding 75% ethanol into a multi-functional ultrasonic extraction tank to extract for three times; combining extraction liquid and concentrating the extraction liquid until no ethanol exists; filtering and clarifying the extraction liquid and the concentration liquid of the cistanche by using ultrafiltration equipment; performing absorption on the liquid by using a macroporous resin column; firstly washing the liquid with water until the liquid is colorless, then eluting the liquid by using ethanol with certain concentration; collecting an elution solution that contains the echinacoside and the verbascoside; concentrating the elution solution by using an outer circulating vaccum concentrator; carrying out spray-drying on the concentration liquid to obtain the cistanche extract; and carrying out efficient liquid-phase tracing detection on the cistanche extract, wherein the echinacoside accounts for greater than or equal to 25%, and the verbascoside accounts for greater than or equal to 10%. The total content of phenylethanoid glycosides is greater than or equal to 80%, and the yield is 20%.

The invention discloses application of an osmanthus phenylethanoid glycoside extract in preparing anti-aging drugs or health products. The total phenylethanoid glycoside content in the osmanthus phenylethanoid glycoside extract is 30-90 wt%, and the total phenylethanoid glycosides comprise verbascoside and isoverbascoside. The verbascoside content in the osmanthus phenylethanoid glycoside extract is 25-80 wt%, and the weight ratio of the verbascoside to the isoverbascoside in the total phenylethanoid glycosides is 48:1-300:1.

The invention provides a production method, which can enable the content of Phenylethanoid Glycoside combination before and after the processing to keep stable through improving the processing treatment technology of a broomrape decoction piece, wherein, the Phenylethanoid Glycoside combination is the active ingredient abundant in broomrape. Through a high-frequency microwave electrical field, the invention leads a polar molecule in a succulent stem cell of a waterrich fresh broomrape to perform high-frequency swing, vibration, and intermolecular friction and extrusion to cause the inner and surface temperature of a broomrape cell to rise rapidly; the pyrometric effect for inactivating the enzyme activity, so as to effectively block and restrain the degradation loss of the Phenylethanoid Glycoside combination caused by the enzymatic reaction induced by enzyme systems such as hydrolytic ferment, glucoside hydrolase, etc., abundant in a fresh broomrape cell; at the same time, the broomrape decoction piece is dried through the technology for microwave drying or other drying methods. The analysis determination shows that the content of echinacoside and a verbascoside monomer compound which are the main index components for weighing the quality of the broomrape decoction piece in the acquired broomrape decoction piece can reach 13 percent to 24 percent (by dry matters), and the water content is 1.3 percent to 4.1 percent.

The invention relates to an integrated ultrasonic cyclic extracting and separating technology for a cistanche functional ingredient. The integrated ultrasonic cyclic extracting and separating technology is characterized by comprising the step of carrying out integrated extraction and subsequent separation on the main effect ingredients, such as phenylethanoid glycosides, polysaccharide and total oligosaccharide of the cistanche by an ultrasonic reinforced extraction technology. By adopting the technology, the comprehensive utilization problem of the cistanche resource is solved; the cistanche extracting efficiency is improved; the production cost is reduced; and the integrated ultrasonic cyclic extracting and separating technology has good industrial production and market development prospects.

The invention relates to the technical field of flexible nano-liposome freeze-dried powder and a preparation method thereof, in particular to flexible nano-liposome freeze-dried powder prepared by cistanche tubulosa extract phenylethanoid glycosides and a preparation method thereof. In preparation, the flexible nano-liposome freeze-dried powder prepared by cistanche tubulosa extract phenylethanoid glycosides comprises the raw materials of the cistanche tubulosa extract phenylethanoid glycosides, lecithin, cholesterin and sodium cholate. Cistanche tubulosa extract phenyylethanoid glycosides are prepared into the nano-liposome freeze-dried powder for the first time, the transdermal absorption effect of cistanche tubulosa extract phenylethanoid glycosides is improved, meanwhile, the action time of cistanche tubulosa extract phenylethanoid glycosides is prolonged, and according to the features that the flexible nano-liposome freeze-dried powder prepared by cistanche tubulosa extract phenylethanoid glycosides has good encapsulation efficiency and is small in particle size and good in stability, technical bases are provided for development of cosmetics or related products with cistanche tubulosa extract phenylethanoid glycosides being main components at the later period.

The invention discloses a double-water-phase extraction method for cistanche deserticola phenylethanoid glycosides. The method comprises the following steps: 1) adding a cistanche deserticola raw material powder into a double-water-phase system; 2) extracting; and 3) transferring a phenylethanoid glycosides enriching phase, concentrating and drying, so as to obtain the cistanche deserticola phenylethanoid glycosides. The method for extracting phenylethanoid glycosides from cistanche deserticola is simple, high in extraction yield, easy for amplified continuous production operation, low in energy consumption, high in phenylethanoid glycosides content in the extract product, and overcomes the disadvantages that conventional method for extracting phenylethanoid glycosides from cistanche deserticola is tedious in operation, time-consuming, low in extraction yield, not high in extracted phenylethanoid glycosides quality, and the like.

The invention provides a preparation method of a molecularly imprinted membrane for separation of phenylethanoid glycosides. The method includes the steps of: adding acteoside and a functional monomerinto a solvent, performing sealing, conducting mixing, and carrying out pre-polymerization; and adding ethylene glycol dimethacrylate and azodiisobutyronitrile into the pre-polymerized system to obtain a polymerization system, immersing a PVDF membrane into the polymerization system, carrying out nitrogen bubbling deoxygenation, then carrying out polymerization reaction to obtain an imprinted membrane, and eluting template molecules in the imprinted membrane with an eluent to obtain the molecularly imprinted membrane. According to the invention, by applying the molecularly imprinted membraneto extract active ingredients in cistanche tubulosa, the adsorption capacity of acteoside reaches 62.82mg / g, the adsorption capacity of echinacoside reaches 29.49mg / g, and the selectivity is 2.74. Inpermselectivity experiment, the permeability coefficient of acteoside is 4.02*10<-6>cm<2> / s, the permeability coefficient of echinacoside is 1.07*10<-5>cm<2> / s, and the permeation selectivity factor is 2.65.

The invention relates to the field of natural drug and specifically relates to a callicarpa nudiflora extract containing phenylethanoid glycoside and a preparation method thereof. The preparation method comprises the following steps of: heating callicarpa nudiflora leaves with water, carrying out reflux extraction, filtering the extracting solution, concentrating, adding a clarifying agent for clarifying the concentrated solution, enabling the clarified solution to pass through a macroporous resin column, firstly eluting with pure water, then, eluting with alcohol with low-concentration alcohol, finally, eluting with high-concentration alcohol, collecting the high-concentration alcohol eluant and drying, thus obtaining the callicarpa nudiflora extract. In the callicarpa nudiflora extract provided by the invention, total phenylethanoid glycoside content is up to 60-80%, and the sum of contents of three constituents including forsythiaside B, verbascoside and isoacteoside is up to 50%-70%.

The invention relates to application of a phenylethanoid glycoside compound and a composition thereof in preparation of a medicine for preventing and treating new coronal pneumonia. Specifically, the invention relates to application of a phenylethanoid glycoside compound and a pharmaceutical composition thereof as a 2019 novel coronavirus (SARS-CoV-2) 3CL protease inhibitor in preparation of drugs for treating and / or preventing and relieving respiratory tract infection, pneumonia and other related diseases caused by 2019 novel coronavirus infection.

The invention relates to a method for extracting phenylethanoid glycoside and flavonoid C-glycoside from corallodiscus flabellate, effectively solving the problem of extraction of the phenylethanoid glycoside and the flavonoid C-glycoside from the corallodiscus flabellate. The method comprises the following steps: taking a Chinese herb corallodiscus flabellate; adding ethanol, and performing reflux extraction; filtering; decompressing filtrate, and recycling a solvent; drying the filtrate in vacuum to obtain an extract for use; taking macroporous adsorption resin, and pretreating the macroporous adsorption resin; dynamically adsorbing the extract by the pretreated resin column; after complete adsorption, rinsing the resin column with distilled water, and eluting for impurity removal; after the impurity removal, eluting with ethanol till eluent is colorless; and collecting the eluent, thus obtaining the phenylethanoid glycoside and the flavonoid C-glycoside. The method is simple, easy to operate, low in extraction cost and high in extraction efficiency.

The invention relates to the technical field of healthcare food, and particularly discloses a chitosan-coating sweet-scented osmanthus phenylethanoid glycosides lipidosome and a preparation method ofthe chitosan-coating sweet-scented osmanthus phenylethanoid glycosides lipidosome. The preparation method adopts soybean lecithin and cholesterol as film materials, adopts an ethanol injection methodto prepare the osmanthus phenylethanoid glycosides lipidosome, and utilizes the chitosan to modify the lipidosome, thereby obtaining the chitosan-coating sweet-scented osmanthus phenylethanoid glycosides lipidosome. The embedding rate of the chitosan-coating sweet-scented osmanthus phenylethanoid glycosides lipidosome prepared by the invention is 35.04 to 88.10 percent, and a particle size is 74.14 to 116.47 nm. The stability of the sweet-scented osmanthus phenylethanoid glycosides is improved, and the sweet-scented osmanthus phenylethanoid glycosides has a sustained release effect.

The invention relates to the technical field of a construction method of an animal model and provides a construction method of a skin pigmentation animal model. The construction method comprises: step 1, selecting a guinea pig to be tested; step 2, shaving long hairs and removing short hairs; step 3, irradiating by ultraviolet rays to obtain the skin pigmentation animal model. By selecting the ultraviolet rays to radiate a back skin of the guinea pig to be tested and taking change conditions of skin melanin and melanocytes as indexes, the skin pigmentation animal model is established through determining an optimal irradiation dosage and irradiation time; the skin is not scorched and the skin has no erythema, molting, swelling and blisters; the skin pigmentation animal model is convenient for microscopic observation and instrument detection; a pigmentation resisting effect of herba cistanche phenylethanoid glycoside nano-emulsion is observed and inspected through appearance evaluation and histopathology; compared with the prior art, physiological statuses of skins of people can be relatively really simulated after skin pigmentation, and references are provided for researches of novel pigmentation-resisting drugs, novel preparations and whitening and sun-screen cosmetics.

The invention relates to a method for identifying forsythia suspensa derivatives. The method comprises the following steps: (1) directly obtaining the forsythia suspensa derivatives as a forsythia suspensa lignans and phenylethanoid glycoside characteristic extract containing an active component group; (2) carrying out IGD (Integrated Graphics Driver) nuclear magnetic resonance spectroscopy fingerprint spectrum detection on the forsythia suspensa lignans and phenylethanoid glycoside characteristic extract so as to obtain peak intensities of characteristic peaks of a plurality of active components in the characteristic extract according to a fingerprint spectrum; and detecting the peak intensity of the characteristic peak of a standard reference corresponding to each active component in the same manner; (3) obtaining the absolute content of the standard reference by a quantitative analysis means; (4) calculating the content of each active component and the content of the active component group in the forsythia suspensa derivatives in a ratio of the peak intensities of the characteristic peaks and the absolute content. The method provided by the invention can be used for reflecting the lignans and phenylethanoid glycoside compounds contained in the forsythia suspensa derivatives and a ratio of the lignans compounds to the phenylethanoid glycoside compounds, so that the purpose of identifying the quality of the forsythia suspensa derivatives can be realized.

A health-care product for delaying senility and relieving fatigue contains the extract of cistanche (containing phenylethanoid glycoside) and ginsenoside. Its advantage is high health-care effect.

The invention provides a plant body building composition which is prepared from the following ingredients in mass percent: 30 to 60% of herba cistanches, 10 to 30% of herba cynomorii, 10 to 25% of astragalus mongholicus bunge and 10 to 20% of rhizoma polygonati. According to different situations, radix ginseng, radix rehmanniae preparata or fructus cnidii can be added. The composition disclosed by the invention richly contains varieties of amino acid, alkaloid, phenylethanoid glycosides, other glycosides and microelements, is beneficial to oxidization resistance, ageing resistance and senile dementia resistance if being eaten / taken for a long time, can improve immunity and enhance memory, further has active effects on protecting liver and protecting angiocarpy, can recover fatigue and enhance human body functions and achieves the body building purpose. Meanwhile, the ingredients of the composition do not contain animal or mineral traditional Chinese medical ingredients, so that a requirement of pure vegetarians is met.

An application based on the extract of Cistanche deserticola, which is used to prepare antidepressant drugs; the extract of Cistanche deserticola is obtained by extracting the herb powder of Cistanche deserticola through liquid reflux to obtain a concentrated solution, and finally separating it by macroporous adsorption resin column chromatography effective part. The effective components prepared by using desert cistanche and cistanche tubulosum through a certain extraction and separation method have good antidepressant effect and can be applied to the preparation and development of antidepressant preparations.

The invention discloses a method for extracting phenylethanoid glycoside substances from cistanche tubulosa and application thereof. The method for extracting the phenylethanoid glycoside substances from the cistanche tubulosa includes the following steps: step 1, performing crushing: crushing dry succulent stems of the cistanche tubulosa, performing sieving, and performing drying; step 2, performing extraction: adding a 50% ethanol aqueous solution into the cistanche tubulosa powder obtained in the step 1, performing heating to the temperature of 70 DEG C, and performing extraction for 2min by using a high shear homogenizing emulsifying machine at the temperature of 70 DEG C, wherein the extraction is performed once; and step 3, performing centrifugation filtration on the extraction liquid obtained in the step 2, performing reduced-pressure drying, and performing vacuum lyophilization to a powder shape, so as to obtain the phenylethanoid glycoside substances. The method provided by the invention has the advantages of short extraction time, a less solvent usage amount and high extraction efficiency, the high-shear equipment is simple and easy to use in series with other equipment,and the method is convenient for realizing automated production.

The invention relates to the technical field of application of a cistanche phenylethanoid glycosides extract, and discloses the application of a cistanche phenylethanoid glycosides extract in preparing drugs for controlling hepatic fibrosis for the first time. The anti-hepatic fibrosis effect of the cistanche phenylethanoid glycosides extract is remarkable, activation and proliferation of hepatic stellate cells can be restrained, collagen synthesis can be reduced, collagen degradation can be promoted, the content of collagen in hepatic fibrosis tissue and the content of hydroxyproline in hepatic tissue can be reduced, and then synthesis of the extracellular matrix is restrained, the anti-hepatic fibrosis function is realized and a new direction is provided for controlling hepatic fibrosis diseases.

The invention discloses a method for simultaneously determining three kinds of phenylethanoid glycoside compositions in a callicarpa nudiflora preparation through HPLC. The method comprises: (1) preparation of a contrast sample solution; (2) preparation of a to-be tested sample solution; and (3) HPLC detection. The method is simple in operation, high in accuracy, good in specificity, relatively high in precision and good in reappearance, and is beneficial for guaranteeing stable quality of the callicarpa nudiflora preparation and clinic curative effect.

The invention provides a method for simultaneous detection of iridoid glycoside, phenylethanoid glycoside, flavone and dicaffeoyl ingredients in lamiophlomis rotate. In the detection method, the peak amount is large, separation degree is high, a base line is stable, the lamiophlomis rotate can be effectively detected, and new selection is provided for guaranteeing of medicine material quality. The iridoid glycoside, phenylethanoid glycoside, flavone and dicaffeoyl ingredients are detected under the same chromatographic condition for the first time, seven specific compounds are identified, and the quality of lamiophlomis rotate medicine materials can be comprehensively and accurately controlled.

The invention discloses a method for extracting phenylethanoid glycoside active components from semenplantaginis. The method comprises extracting by solvents and refining by a chromatography to obtain extract. The obtained extract contains phenylethanoid glycoside active components with a total content above 50%. The method is simple, is suitable for industrialized production, and has a prospect of industrial application.

The invention discloses a rapid qualitative and quantitative detection method for seven phenylethanoid glycoside components in the medicinal material Herba Cistanche. The method can be used for determining the contents of active components in the medicinal material Herba Cistanche. The method comprises the following steps: with echinacoside as a reference substance, establishing relative calibration factors for echinacoside and verbascoside / isoacteoside / cistanoside C / cistanoside A / 2'-acetylverbascoside / 6'-acetylverbascoside by using a percent absorption coefficient; and then carrying out detection by using liquid chromatography, qualitatively determining the components according to relative retention time of each chromatographic peak and the peak of the reference substance, and quantitatively determining the components according to the peak areas of the components and the relative correction factors. Therefore, the method can simultaneously determine the contents of seven phenylethanoid glycoside components including echinacoside in the medicinal material Herba Cistanche by using only one reference substance, i.e., echinacoside.

The invention belongs to the field of Chinese medicine and particularly provides a composition for treating juvenile herpangina. The composition is prepared from, by weight, 40-50 parts of callicarpanudiflora general flavone, 10-20 parts of callicarpa nudiflora general phenolic acid, 20-30 parts of callicarpa nudiflora general phenylethanoid glycosides and 2-6 parts of callicarpa nudiflora polysaccharide. The invention also discloses application of the composition. The four components are all extracted from callicarpa nudiflora, are definite and cooperate with one another to take an effect, and the composition has a great effect of treating and / or preventing juvenile herpangina.