26 results about "Phenylethanoid" patented technology

The invention discloses a preparation method and application of a callicarpa macrophylla extract containing phenylethanoid glycosides, and the preparation method comprises the following steps: (1) mixing callicarpa macrophylla and a 50%-95% ethanol water solution according to a mass-volume ratio of 1: (6-10), pulping, and carrying out ultrasonic extraction to obtain an extracting solution; (2) performing vacuum concentration on the extracting solution obtained in the step (1) to obtain extract; and (3) dissolving the extract obtained in the step (2) with a 50% ethanol aqueous solution, separating and purifying with macroporous resin, and performing vacuum concentration and freeze drying to obtain the callicarpa macrophylla extract. As a low-toxicity and safe natural active ingredient, the callicarpa macrophylla extract prepared by the invention can be added into a skin care product as a functional additive to achieve the effects of resisting aging, relieving, repairing and the like, the application potential of the callicarpa macrophylla in the field of cosmetics is expanded, and high-value utilization of plant resources is promoted.

The invention relates to the technical field of cistanche deserticola detection, in particular to a method for simultaneously and rapidly detecting four phenylethanoid glycosides in cistanche deserticola, which comprises the following steps: preparing a plurality of parts of mixed reference substance solutions containing four phenylethanoid glycosides with known concentrations; preparing a to-be-detected cistanche sample into a test solution; spotting the mixed reference substance solution and the test solution on the same thin-layer plate, carrying out thin-layer chromatography development by adopting a developing solvent, and volatilizing the solvent; placing the thin-layer plate after the solvent is volatilized under ultraviolet light to collect spot images, and processing the images by adopting image analysis software to obtain integral gray values of all phenylethanoid glycoside spots; respectively drawing a standard curve by taking the known concentrations of the four phenylethanoid glycosides in each mixed reference substance solution as horizontal coordinates and the corresponding integral gray values as vertical coordinates, establishing a linear regression equation, and calculating the contents of the four phenylethanoid glycosides in the cistanche deserticola sample to be detected; the invention provides a rapid and accurate method for determining the content of four phenylethanoid glycosides in cistanche.

The present invention relates to myconoside-containing extracts obtained from in vitro systems of plants belonging to the genera Haberlea and Ramonda, including Haberlea rhodopensis Friv, Ramonda heldreichii (Boiss.) C.B. Clarke, Ramonda myconi (L.) Rchb., Ramonda nathaliae Pancic & Petrovic, and Ramonda serbica Pancic, as well as hybrids thereof, belonging to the family Gesneriaceae, and to methods for their preparation. Furthermore, the present invention relates to the use of said extracts as chemoprotective, radioprotective, and UV-protective agents in human and veterinary medicine, dietary supplements, and cosmetics. The described in vitro plant extracts contain two fractions, Extract 1, a polyphenol fraction containing primarily flavone C-glycosides and phenylethanoids (PHEs), and Extract 2, a fraction containing primarily phenylethanoid myconosides, particularly myconosides and pauciflosides, either alone or in combination. Extract 1 contains 0.1-55.0 wt% PHEs, with free phenols, sugars, organic acids, fatty acids, amino acids, and sterols accounting for up to 100 wt%. Extract 2 contains 55.0-99.9 wt% PHEs (particularly myconosides and pauciflosides), with phenolic compounds, monosaccharides, disaccharides, or their residues accounting for up to 100 wt%. Extracts 1 and 2 obtained according to the present invention consist primarily of broad-spectrum RHEs (particularly myconosides and pauciflosides) within the limits of quantitation. These compounds, especially the myconosides, have the high biological, antioxidant, antimutagenic and anticlastogenic activity demonstrated herein and are therefore suitable for use alone or in combination by dilution and by metered formulation for the preparation of products to be used as natural chemical, radiological and UV protection agents with a controlled degree of protection.The latter are used in human and veterinary medicine, dietary supplements and cosmetics for the prevention and treatment of harmful chemical, radiation and ultraviolet effects.

The present invention describes cannabinoid formulations that combine cannabinoids, such as CBD, with other active agents within the phenylethanoid class of compounds, such as oleocanthal, which, in combination, provide synergistic anti-inflammatory effects. These combination preparations are capable of increasing anti-inflammatory effects when compared to each individual compound alone, and are further capable of delivery through a variety of administration routes.

The invention discloses phenylethanoid glycoside 6 '-O-glycosyl transferase FsGT61 / FsGTH7-64 / FsGTH7-69 / FsGT3, and application of the phenylethanoid glycoside 6'-O-glycosyl transferase FsGT61 / FsGTH7-64 / FsGTH7-69 / FsGT3 in catalyzing glycosylation of glucose 6 '-position rhamnose / xylose / arabinose / celery of various phenylethanoid glycosides. The F144H / A / S / P mutant obtained by engineering FsGT61 can catalyze the glucosylation at the 6'position of phenylethanoid glycoside glucose, and a feasible method is provided for generating phenylethanoid glycoside with glycosyl at the 6 'position through in-vitro enzyme catalysis.

Provided are a phenylethanoid glycoside extract from the plant A. ilicifolius L., a preparation method thereof, and its use as an anti-liver injury medicament. The preparation method of the phenylethanoid glycoside extract comprises following steps: pulverizing dried whole plant Acanthus ilicifolius L. and passing through 20-30 mesh sieve to obtain Acanthus ilicifolius L. powder, adding 50%-95% ethanol solution whose mass is 8-20 times of the powder, soaking and extracting under reflux, filtering and collecting extract of the powder, and extracting a filter residue under reflux with 50%-95% ethanol solution whose mass is 8-10 times of the filter residue and collecting extract of the filter residue, combining, concentrating and subjecting to macroporous resin column chromatography, eluting successively by 10%, 30% and 50% ethanol solutions for four column volumes, respectively, collecting fraction eluted by the 50% ethanol solution and drying to obtain the phenylethanoid glycoside extract.

The application provides a molecular imprinting composite film for selectively separating phenylethanoid glycosides, wherein the matrix of the molecular imprinting composite film is polypropylene, polyethersulfone, polycarbonate, cellulose acetate or polyvinylidene fluoride; and a verbascoside imprinting layer or a trilobatin imprinting layer is synthesized on the surface of the composite film. Compared with the prior art, the application has the following advantages: the composite film provided by the application has a coral-like structure, and has the advantages of easy recycling, no pollution, low energy consumption, excellent separation performance, application in continuous separation process and the like, and effectively solves the problems of few types, low purity, difficult recycling, high energy consumption and secondary pollution; the composite film is a molecular imprinting composite film for efficiently separating phenylethanoid glycosides, which is designed by combining the molecular imprinting technology and the membrane separation method in the technical field of separation of effective components of natural products, and is expected to provide a new application idea and method for high-efficiency separation of phenylethanoid glycosides in effective components of natural products.

The invention discloses a compound fermentation technology of herba cistanche and radix astragali and a preparation method of a derivative beverage of the herba cistanche and radix astragali. According to the method, cistanche deserticola and astragalus membranaceus are used as raw materials, ultrasonic-assisted and bionic enzymolysis technologies are firstly adopted for pretreatment, and then a probiotic combination is utilized for fermentation. The content of total flavonoids and phenylethanoid glycosides in the prepared fermentation liquor is obviously higher than that of the raw materials. The fermentation liquor is used as a main material and is compounded with common food ingredients and additives to form drinks with different flavors, so that the requirements of different consumers are met. The process technology is simple and feasible, and industrial production is easy. The herba cistanches and radix astragali compound fermentation liquor and drink prepared by the technology disclosed by the invention not only have good oxidation resistance, but also have the effect of remarkably prolonging the swimming exhaustion time of a mouse with a heavy load, can be used as a functional drink and a health-care product, and have wide application prospects.

The invention discloses a method for preparing high phenylethanoid glycoside liqueur by utilizing cistanche processing byproducts. The preparation method comprises the following steps: by taking desert cistanche processing by-product squamous epidermis as a raw material, performing freeze drying and preliminary crushing, and performing airflow crushing to obtain ultrafine powder of 80-100 meshes; then adopting a three-section gradient extraction process, sequentially performing static high-pressure extraction, ultrasonic-assisted extraction and constant-temperature stirring extraction, and fully enriching phenylethanoid glycoside substances with different polarities; mixing the three sections of extracting solutions according to a specific ratio, compounding with white spirit or yellow wine base, and filtering and clarifying through a filter membrane to obtain a finished product. According to the method, high-valued utilization of cistanche processing byproducts is achieved, loss of active ingredients in the processing process is remarkably reduced, the content of phenylethanoid glycoside substances in the obtained liqueur is high, the processing period is greatly shortened, the liqueur has high extraction rate, high clarity and excellent health-care effects, the process is scientific and controllable, industrial production is facilitated, and the method is suitable for industrial production. The requirements of consumers on the healthy liqueur are met.

This invention provides a plant-based composition comprising a first component, a second component, and a third component. The first component comprises 1-3 parts by weight of protein melanin and 1-3 parts by weight of phenylethanoid glycoside. The second component comprises 10-14 parts by weight of Polygonatum sibiricum powder, 8-12 parts by weight of licorice powder, 8-12 parts by weight of wolfberry powder, 8-14 parts by weight of longan powder, 8-16 parts by weight of jujube powder, 12-15 parts by weight of Poria cocos powder, and 7-10 parts by weight of red rose powder. The third component comprises 3-5 parts by weight of γ-aminobutyric acid (GABA), 2-6 parts by weight of erythritol, 3-5 parts by weight of citric acid, 2-4 parts by weight of sucralose, and 3-5 parts by weight of ascorbic acid. This invention uses protein melanin and phenylethanoid glycoside as the main active ingredients, supplemented with Polygonatum sibiricum powder, Poria cocos powder, and GABA. Experiments have shown that this composition can prolong the swimming time of mice under load and improve their lymphocyte proliferation capacity. This composition can regulate bodily functions and enhance immunity.

The invention relates to the technical field of Chinese patent medicine analysis, in particular to a multi-component characterization method of Jichuan decoction and application of the multi-component characterization method. According to the multi-component characterization method provided by the invention, an off-line two-dimensional liquid chromatography tandem quadrupole-electrostatic field orbitrap high-resolution mass spectrometry technology is adopted, and efficient and accurate analysis of various chemical components of Jichuan decoction is realized through specific chromatographic conditions and mass spectrometry conditions. By means of the method, more than two hundred compounds can be identified from the Jichuan decoction, the compounds contain flavonoids, terpenoids, phenylethanoid glycosides, organic acids, coumarins, phenylpropanoids, iridoids, phthalides, steroids and other components, and reference can be provided for research of pharmacodynamic substances of the Jichuan decoction.

The invention provides a method for extracting and enriching phenylethanoid glycoside and lignan effective components from fructus forsythiae fresh leaves. The method comprises the following steps: extracting phenylethanoid glycoside and lignan compounds, enriching MCI resin components, analyzing by liquid chromatography-mass spectrometry, and identifying. The enrichment extracted by the method provided by the invention is a mixture containing phenylethanoid glycoside and lignan compounds respectively, and can be used as a medicine for resisting the human coronavirus HCoV-229E.

The invention provides a nitrogen-rich triazine COF material, a preparation method thereof and application of the nitrogen-rich triazine COF material in separation and purification of phenylethanoid glycoside compounds, and belongs to the technical field of preparation of functional materials and separation and purification of natural products. According to the invention, based on a network chemical design, 4, 4 ', 4' '-(1, 3, 5-triazinyl-2, 4, 6-triyl) tribenzaldehyde (TFPT) and 2, 4, 6-tri (4-aminophenyl)-1, 3, 5-triazine (TA) are used as construction units, and the COF material with a nitrogen-rich triazine ring structure is formed by self-assembly from bottom to top through a Schiff base reaction; the COF material is mild in synthesis condition, controllable in structure and easy to functionalize; the COF material can be used as an adsorbent and has good application in separation and purification of phenylethanoid glycoside compounds.

The invention relates to the technical field of traditional Chinese medicines, and provides a pharmaceutical composition for enhancing the anti-melanoma effect of an immune checkpoint inhibitor, a preparation and application. The pharmaceutical composition comprises a phenylethanoid glycoside extract and a total terpene extract in a mass ratio of (2-6): 1, the pharmaceutical preparation comprises the pharmaceutical composition and pharmaceutically acceptable auxiliary materials or pharmaceutically acceptable salts. The invention also provides an application of the pharmaceutical composition combined with an immune checkpoint inhibitor in preparation of drugs for treating melanoma. The immune checkpoint inhibitor comprises one or more of a PD-1 inhibitor, a PD-L1 inhibitor, a CTLA-4 inhibitor, a TIM3 inhibitor and an LAG3 inhibitor. The pharmaceutical composition can improve the response rate of a PD-L1 inhibitor and enhance the anti-melanoma effect of an immune checkpoint inhibitor, and an efficient and low-toxicity combined scheme is provided for treating melanoma.

The invention relates to the technical field of traditional Chinese medicine processing, and particularly discloses wine desert cistanche traditional Chinese medicine decoction pieces and a preparation method thereof. The preparation method comprises the following steps: pretreating and spreading cistanche tubulosa, cooling to (-40)-(-30) DEG C at a cooling rate of 10-15 DEG C / min, freezing for 7-9 hours, heating to (-6)-(-4) DEG C at a heating rate of 0.1-0.5 DEG C / min, unfreezing for 7-9 hours, vacuumizing until the vacuum degree is (-0.07)-(-0.05) MPa, and heating to (-10)-(-20) DEG C under the saturated vapor pressure at the temperature of 60-80 DEG C, thereby obtaining the cistanche tubulosa traditional Chinese medicine decoction piece. And carrying out vacuum steam treatment for 10-20 minutes, slicing, spreading and drying, moistening with yellow wine, spreading, steaming, and spreading and drying to obtain the wine desert cistanche traditional Chinese medicine decoction piece. According to the wine cistanche deserticola traditional Chinese medicine decoction pieces obtained through the preparation method, the total phenylethanoid glycoside content is larger than 11 mg / g, the medicine effect is higher, and the market requirement is met.

The application discloses a traditional Chinese medicine extract of Ligustrum lucidum and a preparation method and application thereof. The traditional Chinese medicine extract of Ligustrum lucidum contains total flavones, total iridoid glycosides and total phenylethanoid glycosides, and the content of each of them is greater than or equal to 3% in mass. The application adopts supercritical extraction or subcritical extraction to obtain a Ligustrum lucidum extract which is different from main components of a related product of Ligustrum lucidum which has been marketed at present. The extract has a significant prevention and treatment effect on an animal model related to MASLD, and can be developed into a new traditional Chinese medicine for effectively preventing and treating MASLD.

The invention discloses cistanche enzyme and a preparation method thereof, and belongs to the technical field of food fermentation. The problems that in existing cistanche enzyme preparation, active ingredients are seriously lost, flora metabolism is unbalanced, the quality is unstable, and the flavor is poor are solved. A specific raw material formula and a specific preparation process are adopted; the raw materials comprise 30-50 parts of herba cistanche, 8-20 parts of an antioxidant protective agent consisting of at least two of fructus lycii and the like, and 10-25 parts of a flora adaptive slow-release carbon source consisting of at least two of red dates and the like, and are matched with a compound fermentation strain of lactobacillus plantarum, saccharomyces cerevisiae and acetic acid bacteria; the preparation process comprises the steps of segmented temperature control and dark fermentation, accurate pH adjustment, standard aging and low-temperature sterilization and sterile filling. The total content of phenylethanoid glycoside components is larger than or equal to 0.5 mg / mL, the loss rate is smaller than or equal to 10%, the activity of superoxide dismutase is larger than or equal to 100 U / mL, the viable count of lactic acid bacteria is larger than or equal to 1 * 10 CFU / mL, core functional components of cistanche deserticola and the stability of a fermentation system are reserved, the flavor and storage safety of products are improved, and high-value utilization of cistanche deserticola resources is achieved.

The invention discloses a phenylethanoid glycoside compound as well as a preparation method and application thereof. The phenylethanoid glycoside compound is hydroxytyrosyl-1-O-beta-D-glucoside, and is a new compound separated from hance brandisia herb for the first time. The invention further discloses a preparation method of the compound. The preparation method comprises the steps of alcohol solvent extraction, liquid-liquid extraction, multiple times of silica gel column chromatography, Sephadex LH-20 gel chromatography purification and the like. Finally, the invention discloses an anti-tumor application of the compound and a pharmaceutical composition containing the compound. An in-vitro antitumor activity test shows that the compound has a remarkable growth inhibition effect on tumor cells (especially liver cancer cells). Therefore, the compound provided by the invention can be used for preparing antitumor drugs and has important development and application values.

The invention discloses a cistanche deserticola phenylethanoid glycoside extraction process optimization method. The method comprises the following steps: extracting and preparing a cistanche deserticola phenylethanoid glycoside dry extract; weighing a proper amount of echinacoside reference substance to prepare a standard substance solution, and calculating the content of phenylethanoid glycoside in the desert cistanche deserticola extract by combining the echinacoside standard curve; the method comprises the following steps: determining the content of the extracted cistanche deserticola phenylethanoid glycoside for multiple times by taking the ethanol concentration, the solid-liquid ratio, the extraction temperature, the extraction time and the extraction times of an extracting solution as dependent variables and taking the cistanche deserticola phenylethanoid glycoside yield as a measurement index, and determining the process condition under the maximum extraction amount as the optimal extraction condition; the optimal extraction process is subjected to reproducible operation. According to the method, the optimal process parameters are determined by measuring the content of the cistanche deserticola phenylethanoid glycoside solution and through a uniform design test, and finally, the reproducible operation is performed under the condition of the optimal process parameters, so that the overall optimization is accurate and convenient.

The invention relates to the technical field of natural medicinal chemistry and medicine, in particular to a phenylethanoid glycoside compound as well as a preparation method and application thereof. The structural formula of the phenylethanoid glycoside compound is shown in the specification. The compound is obtained by separating and purifying Clerodendrum bungei Steud. Medicinal materials for the first time, the compound comprises a phenethyl alcohol fragment, a phenylpropanoid fragment, glucose and apiose, and meanwhile, pharmacological activity results show that the compound is non-toxic to normal cells and has a selective inhibition effect on a plurality of tumor cells.

The invention discloses a medicinal and edible composition with the effects of resisting oxidation and reducing uric acid and application of the medicinal and edible composition. The medicinal and edible composition is mainly prepared from the following components in parts by weight: 6 to 12 parts of tricholoma matsutake modified polysaccharide, 15 to 25 parts of rehmannia iridoid glycoside extract, 15 to 25 parts of fructus forsythiae phenylethanoid glycoside extract and 15 to 25 parts of sophora flower bud polyphenol extract. Through verification, the medicinal and edible composition disclosed by the invention has good oxidation resistance, can remarkably inhibit generation of uric acid, promote excretion of uric acid and reduce blood uric acid, and is beneficial to prevention or alleviation of gout. Therefore, the medicinal and edible composition with the effects of resisting oxidation and reducing uric acid can be applied to preparation of functional foods, health care products or medicines for reducing uric acid.

The application belongs to the technical field of food and traditional Chinese medicine health care wine, and discloses a cistanche deserticola wine and a preparation method thereof. The method realizes directional enzymolysis of cistanche deserticola raw materials through composite enzymes, realizes nano-embedding of active ingredients such as phenylethanoid glycosides and stilbenes by combining with γ-cyclodextrin metal organic framework materials, and completes extraction and stable encapsulation under low-temperature and low-alcohol conditions. The preparation comprises the following steps: after the cistanche deserticola is crushed, cellulase and pectinase are used for synergistic enzymolysis; the in-situ synthesized cyclodextrin metal organic framework material is added into the enzymolysis liquid to realize selective embedding; then, the enzymolysis liquid is mixed with pure grain liquor, and the finished product is obtained after aging and filtration. The obtained cistanche deserticola wine has high light transmittance, high active ingredient content, good stability, improved bioavailability, reduced bitterness and coordinated taste, and solves the problems of low extraction rate, easy turbidity and poor flavor of traditional medicinal liquor.

The invention relates to the technical field of medicines, and discloses a sustained-release solid chewable tablet rich in cistanche phenylethanoid glycosides and a preparation method thereof, the sustained-release solid chewable tablet comprises the following components by weight: 15-30% of a cistanche extract, 20-40% of a sustained-release matrix material, 15-35% of a chewable filler, 3-8% of a natural sweetener, 1-3% of a flavoring agent, 0.5-2% of a flow aid, 0.5-1.5% of a lubricant and 0.2-1% of a natural perfume, through a gradient baking technology, phenylethanoid glycoside active ingredients, especially echinacoside and acteoside, in cistanche deserticola are effectively reserved, meanwhile, release of components such as polysaccharide is promoted, the extraction efficiency is improved, through the scientific proportion of composite slow-release matrix materials, the tablets can slowly release the active ingredients in the body, the stable blood concentration is maintained, and the curative effect is good. The medicine action time is prolonged, the medicine taking frequency is reduced, the bitter taste of cistanche deserticola is effectively covered through scientific compatibility of a natural sweetening agent, a flavoring agent and spices, and the medicine taking compliance of a patient is improved.

The present application belongs to the technical field of biological extraction, and particularly relates to a method for extracting and separating phenylethanoid glycoside from Cistanche. The present application improves the extraction and purification process of phenylethanoid glycoside in Cistanche, and integrates secondary crushing, steam explosion for pretreatment of Cistanche, water extraction coupled with complex enzyme preparation for enzymolysis treatment, which greatly improves the yield of phenylethanoid glycoside. Further, multi-stage membrane filtration, D101 macroporous resin adsorption and other operations are used for purification. After purification, the purity of phenylethanoid glycoside can reach 75.6% at most, which provides higher quality phenylethanoid glycoside products for the application fields of antioxidant, anti-fatigue and the like.

The invention discloses a method for de novo synthesis of phenethyl alcohol compounds, which is characterized in that the de novo synthesis of the phenethyl alcohol compounds is realized by constructing metabolic pathways of the phenethyl alcohol compounds including tyrosol, hydroxytyrosol and salidroside in saccharomyces cerevisiae, and the yield of the hydroxytyrosol and the salidroside is greatly increased through multi-copy expression. Meanwhile, a UDP-glucose synthesis route is introduced, so that the synthesis efficiency of the salidroside is greatly improved; the method is simple in process, easy to industrialize and suitable for popularization and application.