A high-temperature polyphenol oxidase and its application in the treatment of phenolic wastewater

A polyphenol oxidase and application technology, applied in the field of environmental biotechnology applications, can solve the problems of low enzyme activity, low expression of polyphenol oxidase, unfavorable fermentation production, etc., and achieve the effect of improving catalytic efficiency
CN113462701BActive Publication Date: 2021-11-26玉凰生态科技股份有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
玉凰生态科技股份有限公司
Publication Date
2021-11-26

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Abstract

The invention discloses a polyphenol oxidase YHL21 gene and polypeptide sequence. The polyphenol oxidase is confirmed by gene splicing and high-throughput screening of enzyme performance. It has a wide range of temperature resistance and strong high-temperature catalytic performance. Single peptide construction, easy membrane immobilization and other advantages. The present invention also discloses a method for recombinant expression and secretion of polyphenol oxidase YHL21 in Bacillus subtilis strain 168, and a method for using polyvinylidene fluoride (PVDF) membrane to immobilize recombinant polyphenol oxidase on the membrane surface , the membrane-enzyme composite system can stably and efficiently degrade organic substances such as phenol and p-chlorophenol in aqueous solution. The present invention carries out enzyme engineering optimization through gene splicing, and obtains a highly efficient polyphenol oxidase that degrades phenolic pollutants, uses Bacillus subtilis to carry out recombinant secretion expression, and uses PVDF to carry out enzyme immobilization, and improves the enzyme catalytic efficiency.
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Description

Technical field

[0001] The present invention belongs to the field of environmental biotechnology, and more particularly to a high temperature polyphenol oxidase and its application in wastewater treatment of phenolic contaminants. Background technique

[0002] Polyphenol oxidase is a copper-containing ionic oxidase which is first isolated from the active substance that cures the polyphenol oxidation, and is also referred to as polyphenol oxidation enzyme (laccase). Polyphenol oxidase can be synthesized from various cell synthesis, plants, animals, bacteria and fungi can produce polyphenol oxidases, and its oxidized substrate object is very broad spectrum, more than hundreds of different structural or type of complex organic matter, so It is widely used in the fields of food, paper, textile, industrial catalysis, pollutant treatment.

[0003] In the food industry, polyphenol oxidase can be used to treat the phenomenon of aromaine and phenolic substances in alcoholic juice, improve...

Claims

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