Kit for specifically detecting peptidoglycan

Abstract

The invention discloses a kit for specifically detecting peptidoglycan.The kit comprises prophenoloxidase, a chromogenic solution and peptidoglycan standards; in order to avoid influences of other interference factors such as glucan, the kit further comprises an alkali metal hydroxide treating agent or a biological buffer treating agent and a neutralization solution treating agent composed of alkali metal hydroxides and biological buffers; in order to store reaction results for a long time, the kit further comprises an acidic stopping solution.The kit can specifically detect the peptidoglycan in sterile body fluid of a human body, and interference of the interference factors such as the glucan is avoided; the sensitivity and specificity are both high, the coincidence rate of an identification result with blood culture strains is high, and clinical test demands can be met; detection can be conducted through an ordinary microplate reader in a laboratory, and the detection cost is reduced; after system reacting is stopped, the test results can be stored for a long time, repeated measurement can be conducted on the test results by means of the microplate reader again and again; operation is easy, long-time experience accumulation is not needed, and popularization is easy.

Description

technical field [0001] The invention relates to a kit, in particular to a kit for specifically detecting peptidoglycan. Background technique [0002] Bacteremia and sepsis caused by bacterial infection seriously threaten human life and health. In recent years, the incidence of infectious diseases caused by bacterial infections has risen sharply with the rapid expansion of antibiotic abuse, immunosuppression, and immune tolerance. Various critical diseases are on the rise, including malignant tumors and malignant blood diseases, AIDS, diabetes, autoimmune diseases, severe burns and trauma, long-term drug abuse, etc., and the aging trend of the population is accelerating. Patients with these diseases are undoubtedly at high risk of bacterial infection On the other hand, the progress of science and the development of medicine have also caused unavoidable negative effects while improving the curative effect and health, such as iatrogenic immune impairment, including broad-spect...

AI Technical Summary

Problems solved by technology

However, the SLP reagent of Wako Pure Chemicals cannot distinguish between peptidoglycan and dextran, and peptidoglycan and dextran respectively indicate bacterial infection and fungal infection, and the treatment drugs are completely different, and this method cannot be used for human detection ;Immunetics' BacTx? Bacterial Detection Kit has passed the US FDA certification, but it cannot distinguish between peptidoglycan and dextran, and can only detect human blood products (platelets)

The rate microplate method judges the presence or absence and amount of peptidoglycan in the sample according to the change of the color development rate after the sample is added. Standard instrument

(2) It takes a long time to occupy the microplate reader during the detection process

(3) After the reaction of the experimental system is completed, the reaction system cannot be stored for a long time, and the test results cannot be repeated

(4) The naked eye cannot be used to interpret the color rendering results

In addition, the detection method of peptidoglycan also has double-antibody sandwich ELISA, which is currently only used for the detection of peptidoglycan content in some animal samples, because anti-peptidoglycan monoclonal antibodies may only be used The peptidoglycan component of a certain bacterial cell wall is immunized, so it is doubtful whether it can detect all bacteria or at least the peptidoglycan of most bacteria, which means that the sensitivity of this product has yet to be verified and improved

[0005] Compared with blood culture and histopathological methods, which are time-consuming, low-sensitivity, and susceptible to interference, peptidoglycan detection requires other detection methods with the advantages of simplicity, rapidity, accuracy, specificity, and high sensitivity. Supplement, providing a new technical means for rapid and early clinical diagnosis of bacterial infection in human aseptic body fluid

However, the market is still under development, and there are still some serious defects in the existing products on the market. Either the mixed detection of peptidoglycan and dextran cannot specifically detect peptidoglycan, or the sensitivity of the methodology has yet to be verified. Therefore, clinical laboratories urgently need peptidoglycan detection kits with sensitivity and specificity that can meet the needs of bacterial infection diagnosis

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