Bacillus licheniformis and application thereof

A technology of bacillus licheniformis and bacterial solution, which is applied in the direction of application, bacteria, and microorganisms, can solve the problems of no anti-prawn virus disease strains, few strains of bacillus licheniformis, and unstable effects, etc., to enhance the non-specific immune function of prawns , reduce virus infection, and slow down the effect of value-added rate

Inactive Publication Date: 2015-03-04
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But at present, there are few Bacillus licheniformis strains used in the production of prawn farming, and there are deficiencies such as mediocre biological properties, low number of viable bacteria, unstable effect, etc., especially there is no application of anti-prawn virus disease strains.

Method used

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  • Bacillus licheniformis and application thereof
  • Bacillus licheniformis and application thereof
  • Bacillus licheniformis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Screening of Bacillus licheniformis

[0014] The Bacillus lincheniformis LV0005 strain of the present invention is selected from the digestive tract of Litopenaeus vannamei. The collected healthy prawn samples were transported back to the laboratory in inflatable bags filled with seawater. Wipe the body surface of live shrimp with 75% alcohol on the aseptic operation table for disinfection, dissect the live shrimp by aseptic operation, and take out the intestinal tissue of the prawn. After washing the removed intestinal tissue with sterile PBS (pH=7.0) buffer solution for 3-4 times, grind it evenly with a sterile grinding rod. Afterwards, it was appropriately diluted with sterile PBS buffer, and 0.1 ml was taken and spread on the 2216E solid medium, and placed upside down in a constant temperature incubator at 28°C for 16h-20h. Observe the colony shape of the bacteria on the 2216E plate, number the bacteria with the same colony shape, and pick a single colo...

Embodiment 2

[0020] With the 100mL bacillus licheniformis LV005 solution that makes and 10Kg basic prawn feed material (containing fish meal 30%, shrimp meal 10%, soybean meal 25%, peanut meal 25%, flour 3%, corn meal 3%, multivitamin 1% ) mixed to make immune feed containing Bacillus licheniformis LV005. Various raw materials were crushed through a 60-mesh sieve, accurately weighed, and mixed well step by step. Sodium alginate was used as a binder, and an appropriate amount of water was added to extrude it with a small meat grinder to make pellet feed, and dried at 40°C. Dried until the moisture content is below 10%, packed in separate bags, and stored in a refrigerator at 4°C for later use.

[0021] There were more than 300 healthy Litopenaeus vannamei prawns, and the initial average body length of the experimental prawns was 6.3 cm. Two treatment groups were randomly divided into the experimental group and the control group. Each treatment group had 3 parallels, and 50 prawns were cultu...

Embodiment 3

[0024]Containing 1% (V / W) bacillus licheniformis LV005 solution's immunization feed (total concentration of living bacteria ≥ 10 7 CFU / mL) The preparation method is the same as embodiment 1.

[0025] There were more than 300 healthy Litopenaeus vannamei prawns, and the initial average body length of the experimental prawns was 6.3 cm. They were randomly divided into two treatment groups, the experimental group and the control group, and 150 prawns were cultured in each treatment group. The experimental group was fed the immune feed for 4 consecutive days, and the basal feed for 3 days, with a cycle of 7 days until the end of the experiment. The control group was fed the basal feed throughout the experimental period. After 21 days of breeding with immunized feed, the artificial injection infection experiment of WSSV was carried out. Feed was stopped 1 day before infection, and 50 μL of WSSV crude extract (concentration: 0.16 g / mL) was injected into the second abdominal segmen...

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Abstract

The invention aims to provide a bacillus licheniformis and application thereof. The preservation number of a bacterial strain of the bacillus (Bacillus lincheniformis) LV005 is CCTCC M 2013262. The bacillus licheniformis LV005 disclosed by the invention is screened from an alimentary canal of a prawn, and has the advantages of improving the composition and the number of dominant microbial populations in the alimentary canal of the prawn, forming and maintaining good microecological balance of the alimentary canal, and resisting the adhesion and the proliferation of viruses in the alimentary canal through nutrition competition, space competition, bacteriocin secretion and the like. The bacterial strain can activate the expression of prophenoloxidase, superoxide dismutase, lysozyme, heat shock protein, lipopolysaccharide-glucan conjugated protein, signal transduction, transcriptional activation factors and the like of the prawn with relevant pathogenic infection resistant molecules, and retard the proliferation rate of the viruses in the body of the prawn, so that the bacterial strain has various biological functions of improving the activity of the protease, the lipase and the amylase of the prawn, promoting the digestion capacity of the prawn and the digestion for feed nutrients, improving the stress response resistant capacity of the prawn and the like.

Description

technical field [0001] The invention belongs to the field of probiotic screening, and in particular relates to a bacillus licheniformis and an application thereof. Background technique [0002] Bacillus lincheniformis belongs to Firmicutes (low G+C Gram-positive bacteria), Bacilli class (Bacilli), Bacillales (Bacillales), Bacillus family ( Bacillaceae), Bacillus (Bacillus). Bacillus licheniformis is ubiquitous in soil and on the surface of plants, and is also one of the common symbiotic bacteria in the intestines of humans and animals. Bacillus licheniformis is one of the 44 "microorganisms that can be directly fed and generally recognized as safe" announced by the U.S. Food and Drug Administration (FDA) and the American Feed Standards Association (AAFCO) in 1989. It is one of the 16 types of feed-grade microbial additive strains that can be directly fed to animals specified in Bulletin No. 1126 "Catalogue of Feed Additives". Compared with traditional probiotics such as b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/18A23K1/16C12R1/10
CPCC12N1/20C12N1/205C12R2001/10
Inventor 宋晓玲孙艳王秀华李玉宏黄倢
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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