Ruminant micro-ecological functional forage containing lactobacillus acidophilus, and preparation and application thereof
A technology of Lactobacillus acidophilus and functional feed, which is applied in the field of microbial beneficial bacteria in feed, can solve the problems of large investment in equipment, and achieve the effect of simple and easy process
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Embodiment 1
[0062] Embodiment 1, the screening of lactic acid bacteria and performance measurement
[0063] 1.1. Screening yak gastrointestinal lactic acid bacteria to obtain LAg12-7 Lactobacillus acidophilus
[0064] MRS medium: peptone 10.00g, yeast extract 5.00g, beef extract 10.00g, glucose 20.00g, dipotassium hydrogen phosphate 2.00g, triammonium citrate 1.00g, sodium acetate 5.00g, magnesium sulfate 0.80g, manganese sulfate 0.25g , Tween 801ml, dilute to 1000ml with distilled water, pH 6.2-6.4, sterilize at 115°C for 30min.
[0065] Using conventional microbiological methods, 108 strains of lactic acid bacteria were isolated from yak gastrointestinal mucosa, and a strain with excellent performance was screened out through acid production, acid resistance and salt resistance experiments. After physiological and biochemical identification and 16SrDNA molecular identification, the strain was determined to be Lactobacillus acidophilus and named Lactobacillus acidophilus LAg12-7. The st...
Embodiment 2
[0084] Embodiment 2, the method for preparing the composite fermented bacterial agent that ruminant micro-ecological function feed is used
[0085] The composition and weight ratio of the composite fermentation agent are: LAg12-7 Lactobacillus acidophilus lyophilized powder 30.0%, Bacillus subtilis lyophilized powder 50.0%, Saccharomyces cerevisiae lyophilized powder 20.0%. The composite fermented bacterial agent for ruminant micro-ecological function feed is prepared by mixing LAg12-7 Lactobacillus acidophilus freeze-dried powder, Bacillus subtilis freeze-dried powder and brewer's yeast freeze-dried powder evenly under normal temperature conditions.
Embodiment 3
[0086] Embodiment 3, the method for preparing the composite fermented bacterial agent that ruminant micro-ecological function feed is used
[0087] The composition and weight ratio of the composite fermentation agent are: LAg12-7 Lactobacillus acidophilus lyophilized powder 60.0%, Bacillus subtilis lyophilized powder 20.0%, Saccharomyces cerevisiae lyophilized powder 20.0%. The composite fermented bacterial agent for ruminant micro-ecological function feed is prepared by mixing LAg12-7 Lactobacillus acidophilus freeze-dried powder, Bacillus subtilis freeze-dried powder and brewer's yeast freeze-dried powder evenly under normal temperature conditions.
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