Ruminant micro-ecological functional forage containing lactobacillus acidophilus, and preparation and application thereof

A technology of Lactobacillus acidophilus and functional feed, which is applied in the field of microbial beneficial bacteria in feed, can solve the problems of large investment in equipment, and achieve the effect of simple and easy process

Inactive Publication Date: 2013-02-27
SOUTHWEST UNIVERSITY FOR NATIONALITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yan Qitao et al. (2000) used photosynthetic bacteria (PSB) to ferment corn yellow slurry water to produce PSB bacteria slime (Run Qitao et al., Application Prospect of PSB Resource Development of Corn Starch Yellow Syrup Water), Journal of Jilin Grain College, 2000 Phase 4), this technology needs to separate the fermentation liquid from the bacterial liquid to make the liquid discharge up to the standard, but it also requires a large investment in equipment

Method used

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  • Ruminant micro-ecological functional forage containing lactobacillus acidophilus, and preparation and application thereof
  • Ruminant micro-ecological functional forage containing lactobacillus acidophilus, and preparation and application thereof
  • Ruminant micro-ecological functional forage containing lactobacillus acidophilus, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1, the screening of lactic acid bacteria and performance measurement

[0063] 1.1. Screening yak gastrointestinal lactic acid bacteria to obtain LAg12-7 Lactobacillus acidophilus

[0064] MRS medium: peptone 10.00g, yeast extract 5.00g, beef extract 10.00g, glucose 20.00g, dipotassium hydrogen phosphate 2.00g, triammonium citrate 1.00g, sodium acetate 5.00g, magnesium sulfate 0.80g, manganese sulfate 0.25g , Tween 801ml, dilute to 1000ml with distilled water, pH 6.2-6.4, sterilize at 115°C for 30min.

[0065] Using conventional microbiological methods, 108 strains of lactic acid bacteria were isolated from yak gastrointestinal mucosa, and a strain with excellent performance was screened out through acid production, acid resistance and salt resistance experiments. After physiological and biochemical identification and 16SrDNA molecular identification, the strain was determined to be Lactobacillus acidophilus and named Lactobacillus acidophilus LAg12-7. The st...

Embodiment 2

[0084] Embodiment 2, the method for preparing the composite fermented bacterial agent that ruminant micro-ecological function feed is used

[0085] The composition and weight ratio of the composite fermentation agent are: LAg12-7 Lactobacillus acidophilus lyophilized powder 30.0%, Bacillus subtilis lyophilized powder 50.0%, Saccharomyces cerevisiae lyophilized powder 20.0%. The composite fermented bacterial agent for ruminant micro-ecological function feed is prepared by mixing LAg12-7 Lactobacillus acidophilus freeze-dried powder, Bacillus subtilis freeze-dried powder and brewer's yeast freeze-dried powder evenly under normal temperature conditions.

Embodiment 3

[0086] Embodiment 3, the method for preparing the composite fermented bacterial agent that ruminant micro-ecological function feed is used

[0087] The composition and weight ratio of the composite fermentation agent are: LAg12-7 Lactobacillus acidophilus lyophilized powder 60.0%, Bacillus subtilis lyophilized powder 20.0%, Saccharomyces cerevisiae lyophilized powder 20.0%. The composite fermented bacterial agent for ruminant micro-ecological function feed is prepared by mixing LAg12-7 Lactobacillus acidophilus freeze-dried powder, Bacillus subtilis freeze-dried powder and brewer's yeast freeze-dried powder evenly under normal temperature conditions.

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Abstract

The invention relates to ruminant micro-ecological functional forage containing lactobacillus acidophilus, and a preparation and an application thereof, and belongs to the technical field of applications of biological bacteria in the forage. The invention relates to a preferred LAg12-7 lactobacillus acidophilus, and particularly relates to a compound fermentation bacteria agent containing the lactobacillus acidophilus, and a method for preparing the ruminant micro-ecological functional forage by applying the compound fermentation bacteria agent in the fermentation of foodstuff industrial waste, forage raw materials and auxiliary materials. The LAg12-7 lactobacillus acidophilus has good acid-producing, acid-resisting and antibacterial performances, can be compatible well with bacillus subtilis and saccharomyces cerevisiae, allowing the pH value of the fermentation forage to be lower than 4.0 rapidly, and can inhibit harmful microbes rapidly, enabling beneficial bacteria to be in a dominant state all the time, and the fermentation forage to keep quality for a long time. The ruminant micro-ecological functional forage is simple and practical in process, has low production cost, is convenient for feeding, has long quality guarantee period, can increase digestion rate of daily-ration cellulose and the like, and has good economic benefits.

Description

technical field [0001] The invention belongs to the technical field of the application of microbial beneficial bacteria in feed, relates to a preferred strain of Lactobacillus acidophilus, in particular to a composite fermentation agent containing the Lactobacillus acidophilus, and the composite fermentation agent is used to remove waste from the food industry It is a method for preparing ruminant micro-ecological function feed by mixing the food as feed raw material and auxiliary materials and then fermenting. Background technique [0002] Micro-ecological functional feed is a feed obtained by fermenting relevant feed materials with a variety of beneficial microorganisms. The microorganisms and metabolites in it are consistent with the natural micro-ecological system in related animals and are nutritionally complementary. The micro-ecological functional feed produced by microbial fermentation technology is of positive significance for the development and utilization of feed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/00A23K1/00A23K1/18C12R1/23
CPCY02P60/87
Inventor 郭春华彭忠利罗璠柏雪王永玲
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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