Method for preparing aflatoxin B1 degradation preparation by mixed fermentation

A kind of aflatoxin and mixed bacteria fermentation technology, applied in the field of biochemistry, can solve the problems of limited production capacity, high cost, easy damage to grain and oil, etc., and achieve the effects of mild action conditions, efficient detoxification, and high safety factor

Active Publication Date: 2019-08-27
合肥停弦渡生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The physical removal method is complex in operation, high in cost, low in efficiency, and seriously limits the production capacity; the chemical removal method is easy to destroy grain, oil, feed nutrition, and incomplete detoxification; the degradation cycle of bacteria and fungi is long, and it requires high conditions such as temperature and pH, and enzymes have substrates Specificity and simple catalytic steps, it is difficult for general enzyme preparations to completely remove toxins

Method used

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  • Method for preparing aflatoxin B1 degradation preparation by mixed fermentation
  • Method for preparing aflatoxin B1 degradation preparation by mixed fermentation
  • Method for preparing aflatoxin B1 degradation preparation by mixed fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The seeds of Aspergillus oryzae (strain from Guangdong Microbial Culture Collection Center, strain number CGMCC3.800) and Saccharomyces cerevisiae (strain from Guangdong Provincial Microbial Culture Collection Center, strain number ATCC204508) seeds preserved at low temperature were inoculated in The PDA medium was cultured in a biochemical incubator at 30°C for 72 hours to complete seed rejuvenation. The rejuvenated seeds were transferred to the seed medium, kept on a constant temperature shaker at a temperature of 25° C., and rotated at 200 rpm, and cultivated for 48 hours to complete seed activation. The seed liquid is inoculated on the fermentation medium, and the volume ratio of the seed liquid is Aspergillus oryzae: Saccharomyces cerevisiae=5:1. The total inoculum amount is 5%, aerobic fermentation, ventilation and stirring are maintained, the fermentation temperature is 30°C, and the fermentation period is 96h.

[0027] Extracellular enzymes and activities after...

Embodiment 2

[0031] Seeds of Aspergillus oryzae CGMCC3.800 and Saccharomyces cerevisiae ATCC204508 preserved at low temperature were inoculated in PDA medium respectively, and cultured in a biochemical incubator at 30°C for 60 hours to complete seed rejuvenation. The rejuvenated seeds were transferred to the seed medium, kept on a constant temperature shaker at a temperature of 30° C., and rotated at 200 rpm, and cultivated for 48 hours to complete seed activation. Inoculate the seed liquid into the fermentation medium, and the volume ratio of the seed liquid to Aspergillus oryzae: Saccharomyces cerevisiae=15:1. The total inoculum amount is 10%, aerobic fermentation, keep ventilation and stirring, the fermentation temperature is 30°C, and the fermentation period is 84h.

[0032]Extracellular enzymes and activities after fermentation: pectinase activity 6000u / ml, xylanase activity 3000u / ml, glucose oxidase activity 80u / ml, cellulase activity 900u / ml, polyphenol oxidase activity 3000u / ml ml...

Embodiment 3

[0036] Seeds of Aspergillus oryzae strain number CGMCC3.800 and Saccharomyces cerevisiae ATCC204508 preserved at low temperature were inoculated in PDA medium respectively, and cultured in a biochemical incubator at 30°C for 72 hours to complete seed rejuvenation. The rejuvenated seeds were transferred to the seed medium, kept on a constant temperature shaker at a temperature of 30° C., and rotated at 200 rpm, and cultivated for 48 hours to complete seed activation. Inoculate the seed liquid into the fermentation medium, and the volume ratio of the seed liquid to Aspergillus oryzae: Saccharomyces cerevisiae = 20:1. The total inoculum amount is 10%, aerobic fermentation, ventilation and stirring are maintained, the fermentation temperature is 30°C, and the fermentation period is 84h.

[0037] Extracellular enzymes and activities after fermentation: pectinase activity 8000u / ml, xylanase activity 5000u / ml, glucose oxidase activity 90u / ml, cellulase activity 1000u / ml, polyphenol o...

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Abstract

The invention discloses a technology of producing an aflatoxin B1 (AFB1) degradation preparation by mixed fermentation and application. Microorganisms used by the AFB1 degradation preparation preparedby the invention comprise aspergillus oryzae CGMCC 3.800, extracellular enzyme secreted by saccharomyces cerevisiae ATCC 204508, an amboceptor, an adjuvant and the like. The extracellular enzyme of the preparation comprises working enzyme (glucose oxidase, polyphenol oxidase) capable of degrading AFB1, assisting enzyme (pectinase, xylanase, cellulase) for improving degradation efficiency, and also comprises micromolecule amboceptor for transferring electron, adjuvant and the like. The working enzyme is oxidase, a catalytic process involves electron transfer and free radical generation, the micromolecule amboceptor and adjuvant can carry the electron into the interior of tissue of grain and oil, feed and related products, so as to degrade the AFB1 infected into the interior of tissue, namely multi-path and multi-step catalysis, thus realizing thorough detoxification of the AFB1. The preparation is convenient to use, small in dosage, high in biological security coefficient, and has gooddegradating effect on the AFB1 in mildewed grain and oil, feed and related products.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and relates to a method for preparing aflatoxin B1 degradation preparation by mixed bacteria fermentation. Background technique [0002] Aflatoxin (AFT) is a highly toxic, carcinogenic and mutagenic secondary metabolite produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. The physical and chemical properties of aflatoxin are stable, and it is very difficult to degrade naturally during the production and processing process; aflatoxin has many components, among which aflatoxin B1 (AFB1) is the most toxic, more than 60 times that of arsenic, and carcinogenic The toxicity is more than 4000 times that of benzopyrene, and it is designated as a class IA carcinogen by the World Health Organization. [0003] AFB1 pollution of grain, oil, feed and related products is a global problem. Known methods to remove AFB1 from grain, oil, feed and related products include: physical remova...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12R1/69C12R1/865
CPCC12P1/02C12N1/145C12N1/185C12R2001/69C12R2001/865
Inventor 王林尹若春罗学才谷中华
Owner 合肥停弦渡生物科技有限公司
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