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Paralysation of silkworm larvae

A technology of lepidopteran larvae and method, applied in the field of strong wire drawing

Inactive Publication Date: 2013-12-25
NEFILA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This immobilization method does not require skilled handling of lepidopteran larvae and can be practiced on a large scale

Method used

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  • Paralysation of silkworm larvae
  • Paralysation of silkworm larvae
  • Paralysation of silkworm larvae

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0083] Inject 0.1 ml of hemolymph extracted and exposed to the air for 10 minutes at the root of the first pseudopod on the right side of the silkworm, or inject 0.1 ml of an aqueous solution of 100 ng / μl paralytic peptide.

[0084] Silkworms injected with hemolymph will be paralyzed within 5 minutes, except for some sustained movement of the silkworm's mandibles and thoracic legs. Silkworms injected with hemolymph will remain motionless for several hours and can be spun immediately. Injection of synthetic silkworm paralytic peptide will also immobilize the silkworm quickly, but compared with hemolymph-injected silkworms, it often takes a longer time for the animal to spin silk, and the effect of peptide injection will be longer than that of hemolymph-injected silkworms. Duration is short.

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Abstract

The present disclosure relates to a method and use for immobilising Lepidopteran larvae and a method, device and use for forced silking from an immobilised Lepidopteran larva. A method for immobilising Lepidopteran larvae is disclosed which comprises paralysing the Lepidopteran larvae by at least one of transgenically expressing a polypeptide having paralytic effects in the Lepidopteran larvae, injecting hemolymph, wherein the hemolymph is drawn from Lepidopteran larvae and oxidised prior to the injecting, or injecting a natural or artificial polypeptide having paralytic effects.

Description

technical field [0001] The field of the disclosure relates to a method and use to immobilize Lepidopteran larvae, and a method, apparatus and use for force-drawing silk from immobilized Lepidopteran larvae. Background technique [0002] Lepidoptera are insects. Lepidoptera is one of the most species-rich orders in the world, including three families of moths, butterflies, gnats, and moth-butterfly hybrids. Many species in the order Lepidoptera are economically beneficial because of their important role in the process of pollination of plants, or because of their ability to produce silk, such as silkworms. [0003] Lepidoptera species undergo a process of change known as "holometabolism." The life cycle of Lepidoptera generally includes eggs, larvae, pupae, and adults. Lepidoptera generally reproduce sexually and oviparously, and some species are ovoviviparous. Larvae are the first stage after hatching and have a very different shape and size than adults. The larvae grow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435D01D5/00A01K67/033A01K67/04D01B7/00
CPCC07K14/43586D01D5/00D01D5/12D01F4/02D01B7/00A01K67/04D01B7/04D01B9/00
Inventor 弗里茨·沃华夫亚历山大·伍兹
Owner NEFILA
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