Anticoagulant for prolonging external hemolymph cell life and its application method

A technology of blood anticoagulant and lymphocytes, which is applied in the field of blood anticoagulant and its application to prolong the storage life of blood lymphocytes in vitro, and can solve the problems of inability to maintain the vitality of lymphocytes, and achieve the effect of maintaining vitality and being convenient to use

Inactive Publication Date: 2003-04-23
SHANGHAI BLOOD CENT
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0003] Aiming at the problem that the conventional anticoagulant used for human leukocyte antigen AB typing cannot maintain the vitality of lymphocytes in a blood sample for a relatively long period of time, the present invention provides a blood anticoagulant for prolonging the storage life of blood lymphocytes in vitro and its use method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] In the blood anticoagulant, every 100 milliliters of the anticoagulant contains 3.315 grams of trisodium citrate, 0.03 grams of citric acid, 2.91 grams of glucose, and the rest is water.

Embodiment 2

[0017] In the blood anticoagulant, every 100 milliliters of the anticoagulant contains 3.31 grams of trisodium citrate, 0.05 grams of citric acid, 3.37 grams of glucose, and the rest is water.

Embodiment 3

[0019] In the blood anticoagulant, every 100 milliliters of the anticoagulant contains 3.32 grams of trisodium citrate, 0.01 gram of citric acid, 2.45 grams of glucose, and the rest is water.

[0020] The method of using the blood anticoagulant of the present invention is that after the blood sample is collected, the blood anticoagulant is mixed with the collected blood. When mixing, the ratio of the blood anticoagulant to the blood is 1:10, that is, every 100 milliliters Add 10 ml of anticoagulant to the blood.

[0021] Tests and actual use have proved that the blood anticoagulant of the present invention can prolong the preservation life of lymphocytes after anticoagulation in human extracorporeal blood from the original 4-6 hours to 48-72 hours, and can make human leukocyte antigen serotyped blood samples It is carried out every other day, so that the blood sample is suitable for long-distance transportation between different places, while maintaining the vitality of lympho...

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Abstract

Each 100 ml of blood anticoagulant contains trisodium citrate 3.31-3.32g, citric acid 0.01-0.05g, glucose 2.45-3.37g and the rest is water. The tests show that after the external blood is undergone the process of anticoagulation treatment it can prolong the stroage life of lymphocyte to 48-72 hr. from original 4-6 hr. Said invention also provides its application method.

Description

Background technique [0001] For routine human leukocyte antigen AB typing (HLA-AB), human venous blood is drawn, and heparin or compound sodium citrate preservation solution (ACD) is added to the venous blood as an anticoagulant. Such anticoagulated blood must be used within 4-6 hours to ensure the vitality of lymphocytes. If blood is drawn for more than 10 hours after anticoagulation, some or all of the lymphocytes will die, and the correct human leukocyte antigen cannot be obtained. classification results. [0002] With the continuous improvement of human civilization, more and more people are participating in the voluntary bone marrow donation team, and the China (Shanghai) bone marrow bank is developing rapidly. For the volunteers who sign up for the bone marrow bank, firstly arrange for volunteers to draw venous blood for routine human leukocyte antigen (HLA) blood type test. The number of blood drawn by volunteers is often large. Generally speaking, each time can reach ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
Inventor 张工梁马俊杨颖
Owner SHANGHAI BLOOD CENT
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