Anticoagulant for prolonging external hemolymph cell life and its application method

Abstract

Each 100 ml of blood anticoagulant contains trisodium citrate 3.31-3.32g, citric acid 0.01-0.05g, glucose 2.45-3.37g and the rest is water. The tests show that after the external blood is undergone the process of anticoagulation treatment it can prolong the stroage life of lymphocyte to 48-72 hr. from original 4-6 hr. Said invention also provides its application method.

Description

Background technique [0001] For routine human leukocyte antigen AB typing (HLA-AB), human venous blood is drawn, and heparin or compound sodium citrate preservation solution (ACD) is added to the venous blood as an anticoagulant. Such anticoagulated blood must be used within 4-6 hours to ensure the vitality of lymphocytes. If blood is drawn for more than 10 hours after anticoagulation, some or all of the lymphocytes will die, and the correct human leukocyte antigen cannot be obtained. classification results. [0002] With the continuous improvement of human civilization, more and more people are participating in the voluntary bone marrow donation team, and the China (Shanghai) bone marrow bank is developing rapidly. For the volunteers who sign up for the bone marrow bank, firstly arrange for volunteers to draw venous blood for routine human leukocyte antigen (HLA) blood type test. The number of blood drawn by volunteers is often large. Generally speaking, each time can reach ...

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problem that the conventional anticoagulant used for human leukocyte antigen AB typing cannot maintain the vitality of lymphocytes in a blood sample for a relatively long period of time, the present invention provides a blood anticoagulant for prolonging the storage life of blood lymphocytes in vitro and its use method