Method for estimating toxicity contribution rate of metal nanoparticle ions and nano effect

Abstract

The invention discloses a method for estimating the toxicity contribution rate of metal nanoparticle ions and a nano effect and belongs to the field of artificial metal nanoparticle toxicity effect evaluation. Hemolymph cells of shellfish in an ocean are selected as an experimental subject, cell culture mediums containing metal ions or nanoparticles are set as two experimental groups according to the content of metal ions released by the metal nanoparticles, and the toxicity contribution rates of an ion effect and the nano effect to the metal ions and the nanoparticles are researched. The nano effect and the ion effect on the metal nanoparticles are researched for the first time, the hemolymph cells of the immune system of a benthic organism in the ocean are selected as the experimental subject, and the cells are very sensitive to stress of pollutants; besides, multiple physiological indexes are selected, the research level is pushed to the molecular immune level, and the effectiveness and the accuracy of toxicity effect evaluation on artificial metal nanoparticles are improved.

Description

technical field [0001] The invention belongs to the field of evaluating the toxicity effect of artificial metal nanoparticles, and in particular relates to a method for estimating the contribution rate of the ion effect and nano effect of metal nanoparticles to the toxicity effect of marine organisms in a marine ecological environment. Background technique [0002] In the past ten years, nanotechnology has developed rapidly, and nano-products, including drug delivery vehicles, conductive accessories, sunscreens, textiles and cosmetics, have increased rapidly. With the continuous expansion of the range of nano-products, it is expected that its annual output may increase to 58,000 tons in the next ten years. This will lead to the increase of artificially synthesized nanoparticles (Engineered Nanoparticles, ENPs) released into the environment year by year, which will bring potential harm to the ecosystem and ecological environment. Artificial nanoparticles refer to materials t...

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Problems solved by technology

[0005] At present, the research on the toxic effect of metal nanoparticles is mainly aimed at the overall toxic effect, but many metal nanoparticles can release metal ions to varying degrees, and the toxic action mode of metal ions has a great difference with metal nanoparticles. Therefore, in order to understand the toxic effects of metal nanoparticles on organisms and to evaluate their toxic effects, it is necessary to separate the ionic effects and nano-effects and study them separately in order to better study the toxic mechanism of metal nanoparticles

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