The invention discloses a fish vision and temperature response research device and method. A fish tank is arranged in a display combined surface which is consistent with the appearance of the fish tank, and a left camera, a right camera and an adjustable light source are vertically downwards arranged right above the fish tank. A video stimulation image is created on a display device, the brightness of the display device is controlled, and the temperature is controlled in a way that a resistance wire is used for heating, and cold water is used for reducing the temperature. A chemical storage controller is used for controlling the chemical charging. The fish vision and temperature response research device is controlled by a control circuit board comprising a system control microprocessor, a display device control circuit, a heating control circuit, a refrigerating control circuit, a chemical charging control circuit and a brightness control circuit. A luminescence sensor and a waterproof temperature sensor are used for transmitting data, and a data collection and analysis server is connected with the two cameras and the control circuit board. Under the program control, fish three-dimensional image coordinate data is collected, analyzed and researched under a fish tank three-dimensional coordinate system. By adopting the fish vision and temperature response research device, the behavior of the fish can be widely and further researched, and an important significance on researching the life science can be realized.