40results about How to "Research results are reliable" patented technology

The invention discloses a highway long-tunnel exit section daytime illumination optimizing method, which comprises the following steps: collecting tunnel lighting parameters, visual adaptation time and driver pupil area data; establishing a driver pupil area and illumination model, an illumination and tunnel length model, and a driver visual adaptation model; working out a three-dimensional curved surface according to relationship among the driver pupil area, the visual adaptation time and the corresponding environment illumination by utilizing software; finally establishing a function model as per parameters obtained from the curved surface, and computing to solve the relationship among tunnel exit distance, car speed and tunnel optimized illumination. The method can solve the problem of dark adaptation caused by illumination difference during traveling in a tunnel at night, reduces traffic accidents induced by visual disturbance, and meanwhile can greatly reduce power consumption of lighting facilities in the tunnel.

The invention relates to an identification method of pathogenicity of fusarium oxysporum sesame special type bacterial strain to host sesame, which comprises the following steps: (1) pretreatment of seeds; (2) bacterial inoculation culture of the seeds: conidium suspending liquid is taken and poured into sterile vermiculite with the volume to be two times that of the conidium suspending liquid, vermiculite mixed with pathogenic bacteria and sterile soil are mixed evenly according to the volume ratio of 1:3 and charged in a split mode, sterile sesame seeds exposing white are sown in a paper bowl, 10 particles are evenly placed in each bowl, each variety is repeated and planted for three times, mixed soil for mixing bacteria is lightly covered on the surface of the seeds and placed into an artificial climate box for light and dark alternated culture for 12 hours per day at 25 DEG C and at the humidity of about 80%, and other culture conditions are the same with equal-volume sterile water as comparison; and (3) performing pathogenicity identification. The identification method is simple, convenient and easy to implement, pathogenicity of the pathogenic bacteria is stable, results are reliable, repeatability is good, and the fact that whether fusarium oxysporum sesame special type bacterial strain has pathogenicity on sesame seedlings and pathogenic strength of the fusarium oxysporum sesame special type bacterial strain can be accurately reflected.

The invention discloses a culture method for rice seedlings. The rice seedlings are cultured by using a split root culture device; the side faces of two culture bowls of the device are butted; a cover plate and a cultivation plate are arranged above the culture bowls; the cultivation plate is provided with culture holes; the culture method comprises the following steps of: culturing 3-day rice seedlings in kimura nutrient solution with concentration of 50 percent for 2 days; replacing the kimura nutrient solution with the kimura nutrient solution with concentration of 100 percent for 12 days;taking consistently grown seedlings and wrapping stem base parts by using culture cotton; fixing in the culture holes of the cultivation plate; grouping rice seedling roots into equal groups A and B,wherein the group A is placed in the culture bowl containing the kimura nutrient solution, and the group B is placed in the culture bowl containing the culture solution of element deficient; adjusting the pH of the culture solution to 6.0 from 8 a.m. to 15 p.m. each day; and replacing the culture solution after culturing for 3 days, and after culturing to 4-12 days, taking the stems and leaves ofthe rice seedlings, the group A of cultured rice seedling roots and the group B of cultured rice seedling roots. The culture method is easy to operate; the personal error is avoided; the culture timeis saved; and the working efficiency is increased.

The invention relates to a Chinese traditional medicinal preparation for treating tumor and process for preparation, wherein the preparation comprises (by weight percent): slice of aconite 1.2-30.5%, pokeberry 1.0-30.3%, notoginseng 1.5-28.9%, Sichuan fritillary bulb 1.9-28.3%, house lizard 3.6-33.1%. The preparation can be made into various dosage forms including capsules, oral liquids and tablets.

The invention provides an identification sequence pair for targeted knockout of Nrf1beta gene in human hepatocyte, Talens, a carrier pair and an application. The identification sequence pair comprises a left arm identification sequence and a right arm identification sequence, wherein a nucleotide sequence of the left arm identification sequence is as shown in SEQ ID NO.1; a nucleotide sequence of the right arm identification sequence is as shown in SEQ ID NO.2; the nuclease Talens comprises a nuclease left arm Nrf1beta-Talen L for identifying the nucleotide sequence of SEQ ID NO.1 and a nuclease right arm Nrf1beta-Talen R for identifying the nucleotide sequence of SEQ ID NO.2; the carrier pair comprises an Nrf1beta-Talen L carrier and an Nrf1beta-Talen R carrier; and the carrier pair can be applied to directed knockout of the Nrf1beta gene in a human hepatocyte line and construction of an Nrf1beta gene knockout type human hepatocyte line.

The present invention provides sisal ACT and GAPDH gene sequences, which can be used as sisal internal reference genes, and simultaneously provide sisal ACT and GAPDH as specific primers for internal reference genes, and establish a qRT-PCR method based on SYBR Green fluorescent dye technology, for Subsequent use of real-time fluorescent quantitative PCR provides stable and reliable internal reference genes and technical methods for gene expression analysis under stress conditions such as Phytophthora sisalum infection, low temperature stress, salt stress, and wound treatment.

The invention discloses a heat simulation method for differential temperature rolling, and belongs to the technical field of metal material technology research. On the basis of Gleeble thermal simulated test machine, an air injection cooling device is additionally arranged inside a working cavity of the thermal simulated test machine. The method comprises the experimental steps of manufacturing, sample grinding, thermocouple welding, differential temperature rolling, quenching treatment and experimental analysis. The heating characteristics of the Gleeble thermal simulated test machine, the conventional sample size is changed, an auxiliary cooling tool is used, and the heat simulation method is used for differential temperature rolling technology research, and convenient and fast research means is provided for mature application of the differential temperature rolling technology.

The invention discloses a compound Chinese traditional medicine preparation for inducing immune tolerance and preparing method and application thereof. The compound Chinese traditional medicine preparation comprises 2-30 parts of roots of Chinese thorowax, 3-31 parts of moutan bark, 4-32 parts of radix paeoniae alba, 5-33 parts of radix curcumae and 6-35 parts of liquorice. The above components are prepared according to the proportion, to be smashed, soaked with water, decocted, filtered, centrifuged and dried to be made into capsules, oral liquid, tablets and the like. The compound Chinese traditional medicine preparation can induce generation of DC regs of high-expression CD11b and low-expression HLA-DR / CD86 / CD80, the DCregs generated through inducing can effectively inhibit DC-mediated lymphopoiesis and promote secretion of cytokines IL-10; a receptor mice is induced to tolerate the iso-allotransplantation skin; no influence on the motility rate of DC cells and the hepatorenal function of the receptor mice is generated, and the compound Chinese traditional medicine preparation is effective and low-toxic and induces immune tolerance.

The invention discloses a method for structuring a hepatitis E infection model, which comprises the following steps of: infecting Meriones unguiculatus with hepatitis E viruses to obtain the hepatitis E infected model. The infection method is oral administration. The method of the invention has low price, adopts the Meriones unguiculatus infection model which is convenient to feed and test, and avoids the defects of high price, limited sources, high experiment requirement condition, long propagation period and the like of non-human primates used at present. The invention provides the ideal infection model for the study of HEV (hepatitis E virus) pathogenesis, vaccine development, interspecies transmission mechanism and the like.