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40results about How to "Research results are reliable" patented technology

Device suitable for studying phototactic ethology of minitype fishes

InactiveCN102613127AAvoid interferenceClearly describe the course of actionPisciculture and aquariaRemote controlFishery
The invention relates to a device suitable for studying phototactic ethology of minitype fishes, and is characterized in that an aquarium box is placed in a dark chamber; the outer part at one end of the aquarium box is provided with an adjustable light source; the bottom of the aquarium box and other parts except for the periphery of the adjustable light source end are not light transmitting; the aquarium box is internally provided with a dividing ruler; the dividing ruler divides the aquarium box into a plurality of regions according to the illumination intensity attenuation direction, and the different regions are divided by a division scale region; a guide rail is arranged deadly above the aquarium box; the guide rail is provided with a plurality of cameras corresponding to the center and the boundary of each region; an underwater illuminometer is suspended on the guide rail, and can move randomly in the aquarium box along the length direction of the guide rail; and a computer remote control terminal is connected with the cameras, and is used for controlling the moving of the cameras and tracking and recording the swimming paths of the fishes. The device suitable for studying phototactic ethology of the minitype fishes provided by the invention can study the phototactic behavior of the minitype fishes, and can remove the interference of artificial factors, thus the studied results are accurate and reliable.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI

Fish vision and temperature response research device and method

The invention discloses a fish vision and temperature response research device and method. A fish tank is arranged in a display combined surface which is consistent with the appearance of the fish tank, and a left camera, a right camera and an adjustable light source are vertically downwards arranged right above the fish tank. A video stimulation image is created on a display device, the brightness of the display device is controlled, and the temperature is controlled in a way that a resistance wire is used for heating, and cold water is used for reducing the temperature. A chemical storage controller is used for controlling the chemical charging. The fish vision and temperature response research device is controlled by a control circuit board comprising a system control microprocessor, a display device control circuit, a heating control circuit, a refrigerating control circuit, a chemical charging control circuit and a brightness control circuit. A luminescence sensor and a waterproof temperature sensor are used for transmitting data, and a data collection and analysis server is connected with the two cameras and the control circuit board. Under the program control, fish three-dimensional image coordinate data is collected, analyzed and researched under a fish tank three-dimensional coordinate system. By adopting the fish vision and temperature response research device, the behavior of the fish can be widely and further researched, and an important significance on researching the life science can be realized.
Owner:SICHUAN UNIV

Culture method for plant seedlings

The invention relates to a culture method for plant seedlings. The plant seedlings are cultured by a ventilated root division culture device, the side surfaces of two culture bowls of the device are in butt joint, a cover plate and a culture plate are arranged on the two culture bowls, culture holes are formed in the culture plate, and vent holes are formed in the two sides of the culture holes. The culture method comprises the followings steps: the plant seedlings of 3 days are taken and cultured in a Hoagland's nutrient solution with the concentration of 50 percent for 2 days then in a Hoagland's nutrient solution with the concentration of 100 percent for 12 days, the plant seedlings growing consistently are taken, the stems of the plant seedlings are wrapped by nutrient cotton and fixed in the culture holes of the culture plate, the roots of the plant seedlings are divided into a group A and a group B, the group A is put into the culture bowl containing the Hoagland's nutrient solution, and the group B is put into the culture bowl containing a nutritional deficiency nutrient solution; the pH values of the nutrient solutions are regulated to 6 at 8 o'clock in the morning and 15 o'clock in the afternoon; the nutrient solutions are changed every other three days; and after 4-12 days, the plant seedlings are taken out. The operation is simple, and the personal errors are reduced, so that the research result is more accurate, the culture time is saved, and the working efficiency is improved.
Owner:HENAN UNIV OF SCI & TECH

Culture method for rice seedlings

The invention discloses a culture method for rice seedlings. The rice seedlings are cultured by using a split root culture device; the side faces of two culture bowls of the device are butted; a cover plate and a cultivation plate are arranged above the culture bowls; the cultivation plate is provided with culture holes; the culture method comprises the following steps of: culturing 3-day rice seedlings in kimura nutrient solution with concentration of 50 percent for 2 days; replacing the kimura nutrient solution with the kimura nutrient solution with concentration of 100 percent for 12 days;taking consistently grown seedlings and wrapping stem base parts by using culture cotton; fixing in the culture holes of the cultivation plate; grouping rice seedling roots into equal groups A and B,wherein the group A is placed in the culture bowl containing the kimura nutrient solution, and the group B is placed in the culture bowl containing the culture solution of element deficient; adjusting the pH of the culture solution to 6.0 from 8 a.m. to 15 p.m. each day; and replacing the culture solution after culturing for 3 days, and after culturing to 4-12 days, taking the stems and leaves ofthe rice seedlings, the group A of cultured rice seedling roots and the group B of cultured rice seedling roots. The culture method is easy to operate; the personal error is avoided; the culture timeis saved; and the working efficiency is increased.
Owner:HENAN UNIV OF SCI & TECH

New method for performing data correction by using cell metabolite relative content as cell number index

The invention discloses a new method for performing data correction by using cell metabolite relative content as cell number index. The new method is characterized by comprising the steps of: realizing the fast quenching of biochemical metabolism in cells by using a simple sample pre-processing method; efficiently extracting small molecule metabolites by an optimized extraction solvent; and roundly and half quantitatively measuring the small molecule compounds in a biological sample by a metabonomics method based on measuring technology like mass spectrum, nuclear magnetic resonance and the like so as to identify a quantitative index of symbolic metabolites in cells to be as the cell number, and regarding the quantitative index as a weight factor to perform data correction. Compared with the quantitative index of the prior cell number, the method is simple, fast and with good repeatability; additional experimental working and the error caused by the additional working are reduced; the accuracy and reliability of experiment result are improved; the difficulty for relatively quantifying the number of in vitro cultured cells is solved; a new displacing method is supplied for cell metabonomics and relevant researches; and the method can be widely used in the relevant researches in the field of cell molecular biology.
Owner:CHINA PHARM UNIV

Method for simulating interfacial interaction of aggregation of nanometer matter in water environment through computer

InactiveCN104077457AObvious superiorityUnderstanding Interface InteractionsSpecial data processing applicationsPollutionMolecular dynamics
The invention discloses a method for simulating interfacial interaction of aggregation of nanometer matter in a water environment through a computer. The method comprises the following steps that (1) a geometric model of interfaces of the nanometer matter in the water environment is constructed, and physical significance is given to the geometric model; (2) the geometric model is optimized with an energy minimization method, and the structure of the geometric model is made to be more authentic; (3) molecule dynamic simulation is conducted under the thermodynamic parameters identical with an authentic environment by referencing experiment researches, and motion locus files and related computing files of all atoms are obtained; (4) the dynamic features and pivotal roles of the interfacial interaction of aggregation of the nanometer matter in the water environment are researched by simulating the obtained motion locus files and the related computing files. According to the method for simulating interfacial interaction of aggregation of the nanometer matter in the water environment through the computer, the potential pollution effects of nanometer pollutants in the water environment are quantitatively analyzed from the angle of interfacial interaction of aggregation of the nanometer pollutants on the level of the molecule and the atoms, and a theoretical basis is laid for water environment biological safety guarantees and the sustainable development of the nanometer technology.
Owner:HARBIN INST OF TECH

Identification method of special type pathogenicity of fusarium oxysporum sesame

The invention relates to an identification method of pathogenicity of fusarium oxysporum sesame special type bacterial strain to host sesame, which comprises the following steps: (1) pretreatment of seeds; (2) bacterial inoculation culture of the seeds: conidium suspending liquid is taken and poured into sterile vermiculite with the volume to be two times that of the conidium suspending liquid, vermiculite mixed with pathogenic bacteria and sterile soil are mixed evenly according to the volume ratio of 1:3 and charged in a split mode, sterile sesame seeds exposing white are sown in a paper bowl, 10 particles are evenly placed in each bowl, each variety is repeated and planted for three times, mixed soil for mixing bacteria is lightly covered on the surface of the seeds and placed into an artificial climate box for light and dark alternated culture for 12 hours per day at 25 DEG C and at the humidity of about 80%, and other culture conditions are the same with equal-volume sterile water as comparison; and (3) performing pathogenicity identification. The identification method is simple, convenient and easy to implement, pathogenicity of the pathogenic bacteria is stable, results are reliable, repeatability is good, and the fact that whether fusarium oxysporum sesame special type bacterial strain has pathogenicity on sesame seedlings and pathogenic strength of the fusarium oxysporum sesame special type bacterial strain can be accurately reflected.
Owner:HENAN ACAD OF AGRI SCI

Estuary ecological water requirement estimation method based on response of shellfish coupled biomarkers

The invention provides an estuary ecological water requirement estimation method based on the response of shellfish coupled biomarkers and belongs to the field of estuary ecological water requirement calculation. The estuary ecological water requirement estimation method comprises the following steps: selecting a type of shellfish scattered within a wide range as the experimental subjects, setting different salinity regions according to a local actual salinity scope, and taking the normal salinity of the estuary as a control salinity region; throwing equal shellfish to the set different salinity regions, measuring the antioxidant system enzyme activity values of samples collected at different points in time and screening out enzyme activity indexes having remarkable response to salinity change; next, standardizing the well-chosen antioxidant enzyme activity values by use of a novel coupled biomarker response method before coupling and determining the salinity region having remarkable difference as a biological response threshold range; and finally, estimating the biological ecological water requirement by use of the difference value of the biological response threshold range and the normal salinity concentration. According to the estuary ecological water requirement estimation method based on the response of the shellfish coupled biomarkers, the shellfish, namely the species having poor motility, is selected and the interference risk is reduced; meanwhile, the research level is promoted to the molecular level and the accuracy of the estuary ecological water requirement calculation is improved.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Ultrasonic auxiliary filtering membrane damage simulation test device and test method

The invention discloses an ultrasonic auxiliary filtering membrane damage simulation test device and a test method. An ultrasonic transducer box is vertically positioned at the right side inside a test pool for dividing the inside of the test pool into a left region and a right region; the ultrasonic transducer box can leftwards and rightwards move and is in sealed contact with the test pool; the left side region is vertically provided with a flat membrane; the flat membrane is clamped by a front side clamp plate and a right side clamp plate; a sound intensity probe is arranged at the front wall part of the back side clamp plate; the center part of the ultrasonic transducer box is connected with the left end of a horizontal screw rod in a rolling way; the right end of the screw rod extends out of the test pool through the right side wall of the test pool and is axially connected with a screw nut with teeth in a sleeving way; the screw nut with teeth is connected with a gear through a belt with teeth; the gear is connected with an output shaft of a step motor in a concentric way. On the premise of the fixed ultrasonic output power of each gear, the rotation of the step motor is precisely controlled by combining an MCU (micro control unit) control system; the ultrasonic intensity is changed through changing the distance between an ultrasonic source and the membrane; the effect that the ultrasonic intensity is roughly adjusted at first and is then finely adjusted is achieved; the obtained rest data is more comprehensive.
Owner:JIANGSU UNIV

Electro-hydraulic loading device based on hydraulic tractor steer-by-wire system

The invention discloses an electro-hydraulic loading device based on a hydraulic tractor steer-by-wire system. The electro-hydraulic loading device is designed for tractors which are relatively slow in development, and complex and changeable in working environment, and is an indispensable importable part for guaranteeing that developing, designing and performance testing can be effectively conducted on the hydraulic steer-by-wire system. According to the electro-hydraulic loading device, the structural characteristic that a single-side single hydraulic cylinder steering trapezium of the hydraulic steer-by-wire system is positioned frontward is utilized for symmetrically arranging a hydraulic loading oil cylinder (1) and a hydraulic steering cylinder (16) of a tractor on the two sides of the trapezium of the steering system, and the ground lateral resistance borne by tires when the tractor steers is simulated through knuckle abutting. The electro-hydraulic proportion control technology which is widely applied at present is adopted in the electro-hydraulic loading device, the effects that loading force changes continuously, quickly and accurately and loading methods are varied can be achieved, the basic requirements of the hydraulic steer-by-wire system are met, and the electro-hydraulic proportion loading device has certain commonality and is applicable to loading of various vehicle steering systems.
Owner:NANJING AGRICULTURAL UNIVERSITY

Identification method of special type pathogenicity of fusarium oxysporum sesame

The invention relates to an identification method of pathogenicity of fusarium oxysporum sesame special type bacterial strain to host sesame, which comprises the following steps: (1) pretreatment of seeds; (2) bacterial inoculation culture of the seeds: conidium suspending liquid is taken and poured into sterile vermiculite with the volume to be two times that of the conidium suspending liquid, vermiculite mixed with pathogenic bacteria and sterile soil are mixed evenly according to the volume ratio of 1:3 and charged in a split mode, sterile sesame seeds exposing white are sown in a paper bowl, 10 particles are evenly placed in each bowl, each variety is repeated and planted for three times, mixed soil for mixing bacteria is lightly covered on the surface of the seeds and placed into an artificial climate box for light and dark alternated culture for 12 hours per day at 25 DEG C and at the humidity of about 80%, and other culture conditions are the same with equal-volume sterile water as comparison; and (3) performing pathogenicity identification. The identification method is simple, convenient and easy to implement, pathogenicity of the pathogenic bacteria is stable, results are reliable, repeatability is good, and the fact that whether fusarium oxysporum sesame special type bacterial strain has pathogenicity on sesame seedlings and pathogenic strength of the fusarium oxysporum sesame special type bacterial strain can be accurately reflected.
Owner:HENAN ACAD OF AGRI SCI

Culture method for rice seedlings

The invention discloses a culture method for rice seedlings. The rice seedlings are cultured by using a split root culture device; the side faces of two culture bowls of the device are butted; a cover plate and a cultivation plate are arranged above the culture bowls; the cultivation plate is provided with culture holes; the culture method comprises the following steps of: culturing 3-day rice seedlings in kimura nutrient solution with concentration of 50 percent for 2 days; replacing the kimura nutrient solution with the kimura nutrient solution with concentration of 100 percent for 12 days;taking consistently grown seedlings and wrapping stem base parts by using culture cotton; fixing in the culture holes of the cultivation plate; grouping rice seedling roots into equal groups A and B,wherein the group A is placed in the culture bowl containing the kimura nutrient solution, and the group B is placed in the culture bowl containing the culture solution of element deficient; adjusting the pH of the culture solution to 6.0 from 8 a.m. to 15 p.m. each day; and replacing the culture solution after culturing for 3 days, and after culturing to 4-12 days, taking the stems and leaves ofthe rice seedlings, the group A of cultured rice seedling roots and the group B of cultured rice seedling roots. The culture method is easy to operate; the personal error is avoided; the culture timeis saved; and the working efficiency is increased.
Owner:HENAN UNIV OF SCI & TECH

A computer-based method for simulating interfacial interactions of nanomaterials aggregated in aqueous environments

InactiveCN104077457BObvious superiorityUnderstanding Interface InteractionsSpecial data processing applicationsPollutionMolecular dynamics
The invention discloses a method for simulating interfacial interaction of aggregation of nanometer matter in a water environment through a computer. The method comprises the following steps that (1) a geometric model of interfaces of the nanometer matter in the water environment is constructed, and physical significance is given to the geometric model; (2) the geometric model is optimized with an energy minimization method, and the structure of the geometric model is made to be more authentic; (3) molecule dynamic simulation is conducted under the thermodynamic parameters identical with an authentic environment by referencing experiment researches, and motion locus files and related computing files of all atoms are obtained; (4) the dynamic features and pivotal roles of the interfacial interaction of aggregation of the nanometer matter in the water environment are researched by simulating the obtained motion locus files and the related computing files. According to the method for simulating interfacial interaction of aggregation of the nanometer matter in the water environment through the computer, the potential pollution effects of nanometer pollutants in the water environment are quantitatively analyzed from the angle of interfacial interaction of aggregation of the nanometer pollutants on the level of the molecule and the atoms, and a theoretical basis is laid for water environment biological safety guarantees and the sustainable development of the nanometer technology.
Owner:HARBIN INST OF TECH

Mouse rearing cage for simulating weightless environment

The invention discloses a mouse rearing cage for simulating a weightless environment. The mouse rearing cage comprises an electromagnet base, a rearing cage and a suspension assembly, an electrified copper coil is embedded in the electromagnet base, and a power plug, a switch button and a current regulation knob are arranged on the outer side wall of the electromagnet base; the rearing cage is of a box body structure with an opening in the upper end, the rearing cage is arranged at the top end of the electromagnet base, a throwing opening is formed in one side wall of the rearing cage, and a placing frame is arranged in the throwing opening; and the suspension assembly comprises a net-shaped top cover and a plurality of suspension magnets, the net-shaped top cover is placed at an opening in the upper end of the rearing cage, and a binding belt is arranged on each suspension magnet. The mouse rearing cage for simulating the weightless environment has the advantages of being adjustable in parameter and weightlessness degree; and the posture problem caused by weightlessness simulation through a traditional tail hanging method can be solved, the influence of non-gravity factors such as blood perfusion caused by the posture problem is avoided, and weightless simulation research can be better carried out.
Owner:SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE

Riparian zone lateral undercurrent exchange process simulation device based on dye tracing

The invention relates to a riparian zone lateral undercurrent exchange process simulation device based on dye tracing. The device comprises an organic glass tank main body composed of an organic glass tank component C and an organic glass tank component G which are vertically arranged in a T shape and are internally connected with each other, a water level control assembly and a dye tracing monitoring assembly, wherein the water level control assembly includes a water supply unit and a water discarding unit which are connected with water inlets and water outlets in the side walls of the organic glass tank component C and the organic glass tank component G; and the dye tracing monitoring assembly includes a camera arranged on the front side through a triangular support and transparent viscous graduated scales attached to front side panels of the organic glass groove component C and the organic glass groove component G respectively, and the dye tracing monitoring assembly is used for observing changes of the water level and the undercurrent exchange range and migration tracks of dye tracers. Compared with the prior art, the device has the advantages of non-intrusive monitoring, fine observation in the whole process, simplicity and rapidness in operation, quantitative description of a dynamic process and the like.
Owner:TONGJI UNIV

Landslide susceptibility evaluation method based on topographic units

The invention discloses a landslide susceptibility evaluation method based on terrain units, solves the problem of poor reliability of an existing landslide susceptibility evaluation method, and belongs to the field of regional geological disaster evaluation. The method comprises the following steps: determining a landslide point position and related disaster-inducing factor data; acquiring discrete intervals of the disaster-inducing factor data, wherein each discrete interval corresponds to a classification category, and the intervals are optimized by adopting intersection points and inflection points of an area classification ratio curve and a weight ratio index curve; carrying out statistics on the optimized discrete interval area and the corresponding landslide point number, and obtaining a slip inclination index; constructing a river network by using a terrain surface model DEM; constructing a terrain unit according to the river network; assigning the slip degree index of each optimization interval of each disaster-inducing factor to each terrain unit; and establishing a random forest model, in which the input is the data of each terrain unit, and the output is the landslide susceptibility probability value of each terrain unit.
Owner:HENAN UNIVERSITY
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