Method for structuring hepatitis E infection model
A technology of hepatitis E and construction method, which is applied in the field of construction of hepatitis E infection model, can solve the problems of inconvenient feeding, long breeding cycle, and high experimental requirements, and achieve simple and easy experimental operation, convenient feeding and management, and avoid expensive effect
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[0016] Embodiment 1, the construction of hepatitis E infection model and its effect verification
[0017] 1. Construction of hepatitis E infection model
[0018] (1) Experimental gerbils (purchased from the Department of Experimental Animal Science, Capital Medical University): use SPF grade or clean grade, 50-80 g long-clawed gerbils. After antibody detection, HEV IgG-negative mice were selected, and 28 mice were selected and divided into two groups, 14 mice in each group, and raised separately in the isolation system.
[0019] (2) challenge: select HEV gene type 4 virus (the sequence of its genome is the sequence shown in FJ409465 numbered in GENBANK) copy number is at 10 5 The above pig liver disease materials were used to prepare liver suspension, and each long-clawed gerbil was orally administered with 10 5 -10 8 copies of virus (genome dose), at 10 6 1 virus copy is the optimal infection dose, in the present embodiment, HEV content is 10 in every milliliter suspensio...
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