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Estuary ecological water requirement estimation method based on response of shellfish coupled biomarkers

A biomarker and water demand technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of reducing the authenticity of research, increasing the risk of research interference, low accuracy, etc., to achieve clear environmental characteristics, Simple calculation method and strong comparability effect

Inactive Publication Date: 2015-03-04
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0005] The current research on biological ecological water requirements is mainly based on the population and distribution changes of fish to calculate the biological ecological water requirements from the group level, but the accuracy of this method is not high
In addition, the swimming nature of fish also increases the risk of interference in the research, reducing the authenticity of the research

Method used

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  • Estuary ecological water requirement estimation method based on response of shellfish coupled biomarkers
  • Estuary ecological water requirement estimation method based on response of shellfish coupled biomarkers
  • Estuary ecological water requirement estimation method based on response of shellfish coupled biomarkers

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Embodiment 1

[0040] A method for estimating the ecological water demand of an estuary based on shellfish-coupled biomarker responses, using the following process steps:

[0041] The test organism, the four-cornered clam, was taken from the tidal flat area at the mouth of the Yellow River (Kenli County, Shandong Province). The water temperature at the sampling point was 19.23°C and the salinity was 20.02. Wash the clams briefly before putting them in, and select the same size and specification (shell length: 2.4-2.6cm; shell height: 1.68-1.49cm; shell width: 2.1-2.3cm, weight with shell: 5.2-6.1g), and the structure is intact , 1,200 four-cornered clams that can move freely.

[0042] The salinity of the mouth of the Yellow River increases sharply after the runoff into the sea decreases in the dry season. According to the hydrodynamic conditions of the sea area, in the salinity fluctuation area (56Km 3 ) within 5 stations (z1, z2, z3, z4 and z5, see figure 1 ), the station salinity ranges ...

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Abstract

The invention provides an estuary ecological water requirement estimation method based on the response of shellfish coupled biomarkers and belongs to the field of estuary ecological water requirement calculation. The estuary ecological water requirement estimation method comprises the following steps: selecting a type of shellfish scattered within a wide range as the experimental subjects, setting different salinity regions according to a local actual salinity scope, and taking the normal salinity of the estuary as a control salinity region; throwing equal shellfish to the set different salinity regions, measuring the antioxidant system enzyme activity values of samples collected at different points in time and screening out enzyme activity indexes having remarkable response to salinity change; next, standardizing the well-chosen antioxidant enzyme activity values by use of a novel coupled biomarker response method before coupling and determining the salinity region having remarkable difference as a biological response threshold range; and finally, estimating the biological ecological water requirement by use of the difference value of the biological response threshold range and the normal salinity concentration. According to the estuary ecological water requirement estimation method based on the response of the shellfish coupled biomarkers, the shellfish, namely the species having poor motility, is selected and the interference risk is reduced; meanwhile, the research level is promoted to the molecular level and the accuracy of the estuary ecological water requirement calculation is improved.

Description

technical field [0001] The invention belongs to the field of estuary ecological water demand calculation, and in particular relates to an estuary ecological water demand estimation method based on shellfish coupled biomarker responses. Background technique [0002] The estuary is the junction of sea and land, where salty and fresh water meet. Its unique natural environment characteristics and ecological regulation function play a very important role in maintaining the global ecological balance. The salinity in the estuary area is often fluctuating due to the influence of upstream river runoff, etc. Under normal ecological conditions, estuarine organisms can adapt to this salinity change through their own regulation. However, in recent years, due to the impact of climatic factors, environmental factors and various human factors in the estuary area, especially in the dry season, the runoff has decreased year by year or even stopped, the salinity of the estuary has risen sharpl...

Claims

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Application Information

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IPC IPC(8): C12Q1/527C12Q1/48C12Q1/30C12Q1/28C12Q1/26
Inventor 孙雪梅夏斌陈碧鹃曲克明崔正国
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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