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36results about How to "Research results are accurate" patented technology

Culture method for plant seedlings

The invention relates to a culture method for plant seedlings. The plant seedlings are cultured by a ventilated root division culture device, the side surfaces of two culture bowls of the device are in butt joint, a cover plate and a culture plate are arranged on the two culture bowls, culture holes are formed in the culture plate, and vent holes are formed in the two sides of the culture holes. The culture method comprises the followings steps: the plant seedlings of 3 days are taken and cultured in a Hoagland's nutrient solution with the concentration of 50 percent for 2 days then in a Hoagland's nutrient solution with the concentration of 100 percent for 12 days, the plant seedlings growing consistently are taken, the stems of the plant seedlings are wrapped by nutrient cotton and fixed in the culture holes of the culture plate, the roots of the plant seedlings are divided into a group A and a group B, the group A is put into the culture bowl containing the Hoagland's nutrient solution, and the group B is put into the culture bowl containing a nutritional deficiency nutrient solution; the pH values of the nutrient solutions are regulated to 6 at 8 o'clock in the morning and 15 o'clock in the afternoon; the nutrient solutions are changed every other three days; and after 4-12 days, the plant seedlings are taken out. The operation is simple, and the personal errors are reduced, so that the research result is more accurate, the culture time is saved, and the working efficiency is improved.
Owner:HENAN UNIV OF SCI & TECH

System and method for realizing synchronous control of multiple targets in deflagration experiment test system

The invention relates to a system and a method for realizing synchronous control of multiple targets in a detonation experiment test system. The system comprises a shock tube, a premixed gas supply system, a flame retardant injection system, a flame signal acquisition system, a pressure signal acquisition system, a data acquisition system, a laser schlieren system, a high-pressure ignition systemand a synchronous control system for controlling the systems, the shock tube comprises a plurality of experiment pipelines and a visual observation window experiment section which are connected in sequence; the premixed gas supply system is communicated with the interior of the shock tube to generate premixed gas with a preset equivalence ratio and input the premixed gas into the shock tube; the flame retardant spraying system is connected with the visual observation window experiment section; the flame signal acquisition system and the pressure signal acquisition system are arranged on the side wall of each section of experiment pipeline; the laser schlieren system is used for measuring distribution images of typical free radical concentration and temperature of the deflagration flow field; the high-pressure ignition system is connected with the end of the shock tube and used for ignition. The system and the method can be widely applied to the field of safety science and technology.
Owner:NORTH CHINA INST OF SCI & TECH

New method for performing data correction by using cell metabolite relative content as cell number index

The invention discloses a new method for performing data correction by using cell metabolite relative content as cell number index. The new method is characterized by comprising the steps of: realizing the fast quenching of biochemical metabolism in cells by using a simple sample pre-processing method; efficiently extracting small molecule metabolites by an optimized extraction solvent; and roundly and half quantitatively measuring the small molecule compounds in a biological sample by a metabonomics method based on measuring technology like mass spectrum, nuclear magnetic resonance and the like so as to identify a quantitative index of symbolic metabolites in cells to be as the cell number, and regarding the quantitative index as a weight factor to perform data correction. Compared with the quantitative index of the prior cell number, the method is simple, fast and with good repeatability; additional experimental working and the error caused by the additional working are reduced; the accuracy and reliability of experiment result are improved; the difficulty for relatively quantifying the number of in vitro cultured cells is solved; a new displacing method is supplied for cell metabonomics and relevant researches; and the method can be widely used in the relevant researches in the field of cell molecular biology.
Owner:CHINA PHARM UNIV

Rocker arm type valve wear test device

The invention discloses a rocker arm type valve wear test device which comprises a rocker arm platform, a rocker arm, a cam, a motor, a combustion chamber, a valve tapered head, a valve, a valve retainer and an impact spring for driving the valve to impact the valve retainer. A valve head of the valve stretches into the combustion chamber, the valve retainer is arranged in the combustion chamber, a valve rod of the valve stretches out of the combustion chamber, the valve tapered head is fixedly connected to the valve rod, the valve rod is sleeved with the impact spring, the two ends of the impact spring are connected with the valve tapered head and the combustion chamber respectively, the middle of the rocker arm is hinged to the rocker arm platform, one end of the rocker arm is the driven end driven by the cam, the other end of the rocker arm is a trigger end for driving the valve rod to move, and the motor is used for driving the cam to rotate. The device is driven by the motor, a roller makes contact with the cam, wear between the cam and the rocker arm is reduced, contact of the rocker arm and the valve is more accurate, and the wear condition produced when the rocker arm makes contact with the valve can be accurately observed.
Owner:SOUTH CHINA UNIV OF TECH

Method for designing, amplifying and sequencing twelve pairs of floccularia luteovirens microsatellite primers

The invention relates to a method for designing, amplifying and sequencing twelve pairs of floccularia luteovirens microsatellite primers. The method comprises the following steps: (1) extracting genome DNA of three floccularia luteovirens populations among which the geographic interval is more than 300km by using an improved CTAB method; (2) randomly selecting an individual genome DNA respectively from the three populations, mixing, detecting the quality of total DNA, preparing a gene library, and performing Illumina HiSeq<TM>2500 sequencing after the gene library is qualified in examination in depot; (3) splicing sequenced data, detecting simple sequence repeats (SSR) in the total DNA sequence by using SR search software, and performing primer design by applying primer3; (4) preparing an SSR primer having an annealing temperature of 50-60 DEG C by adopting a temperature gradient method; (5) respectively performing PCR amplification on the genome DNA of the three floccularia luteovirens populations, and sequencing and verifying to obtain 12 pairs of primers with polymorphism; and (6) calculating the number N of allelic genes, haplotype diversity H<d> genetic differentiation coefficient F<ST>, nucleotide diversity P, G<ST> and the value of pi. The method is beneficial to large-scale research.
Owner:CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST

Root box test device suitable for growing of plants

The invention relates to a root box test device suitable for growing of plants. The root box test device comprises a cylindrical soil storage container and a cylindrical rhizosphere soil storage device, wherein the cylindrical rhizosphere soil storage device is positioned at the center of the cylindrical soil storage container and positioned on a lug boss; the cylindrical rhizosphere soil storage device comprises circular rings, support strips and nylon nets, which are combined to form a net-shaped cylinder with an upwards opening, the circular rings are positioned at two ends of the cylinder, the nylon nets are covered on the surfaces of the lower end and the outer side of the cylinder, and the lug boss is arranged at the center of the bottom surface of the cylindrical soil storage container. The cylindrical rhizosphere soil storage device is in interference fit with the cylindrical soil storage container through the lug boss and the circular rings. Soil is filled in a root box, and crops grow in the rhizosphere soil storage device. According to the root box test device, the problem that plant root systems only grow towards two directions during testing of the root box is solved, and the root systems radically grow towards the circumference so that the simulating test research result is accurate.
Owner:HUAZHONG AGRI UNIV

Method and device for measuring soil penetration characteristic based on instantaneous acceleration detection

The invention discloses a method and a device for measuring a soil penetration characteristic based on instantaneous acceleration detection, specifically a method and a device for measuring soil penetration resistance and then obtaining soil physical properties. The device mainly comprises an acceleration detection device, a handle, a guide rod, impact weights, an adapter sleeve, a penetrating rod, a guide support and the like. The penetrating rod penetrates into soil, the acceleration detection device is used for detecting acceleration change in the descending process of the whole device, and after the whole operation process is finished, data can be read in a computer for further analysis processing. The initial velocities when the penetrating rod penetrates into the soil are different by changing parameters in the descending process, so that the velocities of a conical head reaching the same depth are different, and then the relation between mathematical model discussion velocity and penetration resistance can be established, a series of characteristic parameters of soil are researched, and the device has significance for guiding agricultural production. Speed-changing penetration, automatic recording and data storage are realized, and the device is high in measuring precision and high in work efficiency.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Identification method of special type pathogenicity of fusarium oxysporum sesame

The invention relates to an identification method of pathogenicity of fusarium oxysporum sesame special type bacterial strain to host sesame, which comprises the following steps: (1) pretreatment of seeds; (2) bacterial inoculation culture of the seeds: conidium suspending liquid is taken and poured into sterile vermiculite with the volume to be two times that of the conidium suspending liquid, vermiculite mixed with pathogenic bacteria and sterile soil are mixed evenly according to the volume ratio of 1:3 and charged in a split mode, sterile sesame seeds exposing white are sown in a paper bowl, 10 particles are evenly placed in each bowl, each variety is repeated and planted for three times, mixed soil for mixing bacteria is lightly covered on the surface of the seeds and placed into an artificial climate box for light and dark alternated culture for 12 hours per day at 25 DEG C and at the humidity of about 80%, and other culture conditions are the same with equal-volume sterile water as comparison; and (3) performing pathogenicity identification. The identification method is simple, convenient and easy to implement, pathogenicity of the pathogenic bacteria is stable, results are reliable, repeatability is good, and the fact that whether fusarium oxysporum sesame special type bacterial strain has pathogenicity on sesame seedlings and pathogenic strength of the fusarium oxysporum sesame special type bacterial strain can be accurately reflected.
Owner:HENAN ACAD OF AGRI SCI

Method for detecting irritation of cosmetic raw material and method for detecting anti-irritation of anti-irritation product

ActiveCN110018161AStimulating precisionStimulating objectiveMaterial analysis by optical meansMedicineEye irritation
The invention discloses a method for detecting anti-irritation of an anti-irritation product. The method for detecting the anti-irritation of the anti-irritation product comprises the following stepsof 1) obtaining an image of skin or a skin mimic obtained after patch treatment, wherein a patch is coated with a gel composition comprising the anti-irritation product and an irritant; and 2) measuring an erythema feature value of the skin or the skin mimic in the image. The invention also discloses a method for detecting the irritation of a cosmetic raw material, the gel composition and the patch. The detection method provided by the invention not only has good repeatability, but also can more accurately score the results of irritation antagonism; meanwhile, the subjective influence of people is avoided; and therefore, the irritation antagonism effect of the anti-irritation product is more accurately and objectively evaluated.
Owner:三立慧评(北京)检测技术有限公司

Novel automatic soil sample compaction apparatus and method for root-soil composite

The invention discloses a novel automatic soil sample compaction apparatus for root-soil composite. The novel automatic soil sample compaction apparatus comprises a compaction cylinder, a hammering device, a driving device, a photoelectric induction switch and a control device, wherein a reflecting material is arranged on the inner wall of the compaction cylinder; the hammering device is arrangedin the compaction cylinder and comprises a compaction rod and a compaction hammer; the compaction hammer is arranged on the outer side of the compaction rod in a sleeving manner; the driving device isconnected with the hammering device and is used for driving the hammering device to make a hammering movement; the photoelectric induction switch is arranged on the side wall of the compaction rod and corresponds to the reflecting material; the control device is connected with the driving device and the photoelectric induction switch; and when the photoelectric induction switch induces a signal,the signal is transmitted to the control device, and the control device transmits a command to the driving device to correspondingly execute the action. According to the compaction apparatus disclosedby the invention, automated control of the compaction process can be realized, and the working efficiency is improved; and moreover, with the adoption of automatic insertion, the research result of shear strength of the root-soil composite is accurate.
Owner:WEIFANG UNIVERSITY

Static timing analysis method for thermal stress circuit with silicon through holes

he invention belongs to a static timing analysis method for a thermal stress circuit with silicon through holes. The method comprises the following steps of: (1) determining the types of the silicon through holes used in a circuit; (2) extracting the physical parameters of all layers of materials and transistors in the silicon through holes in the circuit; (3) obtaining the radial stress and hoop stress of all layers of materials of single silicon through hole in a cylindrical-coordinate system; (4) solving the boundary conditions of all coefficients in a stress expression; (5) converting the stress under the cylindrical-coordinate system into the stress under a cartesian coordinate system; (6) obtaining total thermal stress distribution caused by a plurality of silicon through holes according to a linear superposition norm; (7) calculating the influence on the change of carrier mobility in different channel directions; and (8) adding the change of carrier mobility to a Gate-level netlist of the circuit, and operating PrimeTime to carry out the static timing analysis under a timing constraint condition, so as to obtain the conditions of longest path time delay and timing sequence allowance change of the circuit.
Owner:XIDIAN UNIV

Method for establishing disease model of 'human-derived' short QT syndrome

The invention discloses a method for establishing a disease model of 'human-derived' short QT syndrome, specifically for the application of induced pluripotent stem cells (iPSCs) technology to establish a 'human-derived' short QT syndrome (SQTs) disease model. Patient skin fibroblasts from individuals with SQTs disease are reprogrammed to iPSCs, and then differentiated into cardiomyocytes specificto patients with SQTs, to establish a stable model of cardiomyocyte disease in vitro, and use it to analyze disease mechanisms and screen therapeutic drugs; and CRISPR / Cas9 genome editing technologywas used to correct the mutation site of the patient's pathogenic gene, and then the iPSCs were differentiated into cardiomyocytes, and the indexes of iPSCs were detected, which provided a basis for the study of the mechanism of disease.
Owner:ZHEJIANG UNIV

Device and method for monitoring and researching insect taxis behaviors

The invention belongs to the technical field of inset performance monitoring, in particular to a device and method for monitoring and researching insect taxis behaviors. According to the device for monitoring and researching the insect taxis behaviors, by arranging a plurality of monitoring containers and corresponding covers, studies on multi-parameter taxis behaviors can be carried out synchronously, so that parallel comparison among data pieces is convenient; preferably, by arranging a timing device connected with the covers, the corresponding covers are controlled to open and close, and then the studies on the taxis behaviors in all the monitoring containers are controlled. The device for monitoring and researching the insect taxis behaviors can synchronously complete researching and contrasting of different taxis behaviors of multiple reference objects and can be used for automatically monitor and research the taxis behaviors of insects in different time periods, trouble of manualmonitoring is eradicated, and the device for monitoring and researching the insect taxis behaviors is not only simple in structure but also extremely convenient to use.
Owner:INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI

A method for display intraosseous vein-artery blood vessel

The invention discloses a method for display intraosseous vein-arterial blood vessel. The method comprises that follow steps: (1) performing first blood vessel perfusion on bone tissue specimen with perfusion liquid A or perfusion liquid B, performing microCT scan and first three-dimensional reconstruction of intraosseous blood vessel data to obtain first intraosseous blood vessel data; (2) Perfusion solution A or perfusion solution B was used to perfusion the second blood vessel of bone tissue, and the second three-dimensional reconstruction of intraosseous blood vessel data was performed bymicro-CT scanning to obtain the second blood vessel data; (3) Register the first intraosseous blood vessel data and the second intraosseous blood vessel data to obtain the femoral artery blood vesselsystem and femoral vein blood vessel system. The invention is the first, simplest and most time-saving method, the research result is accurate, and can quantify the blood vessel diameter, blood vessellength, blood vessel volume, blood vessel length density, volume density, bone trabecular parameters, and is interrelated with bone mineral content in bone tissue and bone trabecular structure parameters.
Owner:AFFILIATED ZHONGSHAN HOSPITAL OF DALIAN UNIV

Chilo suppressalis breeding method

The invention relates to the technical field of insect breeding, in particular to a chilo suppressalis breeding method. An egg mass is positioned at the intersection of a sheath of a first rice stem and a main stem, and hatched larvae bore through the first rice stem to eat; after the larvae grow to an age of 5, the larvae are taken out from the first rice stem and fed on a second rice stem, wherein the first rice stem refers to a fresh rice stem with roots and the sheath in or after a round stem elongation stage, and the second rice stem refers to a knotted fresh rice stem in or after the round stem elongation stage. The chilo suppressalis breeding method has advantages of high larva and pupa survival rate, convenience in pupa searching, suitableness for researching and availability for large-scale breeding.
Owner:INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI

Identification method of special type pathogenicity of fusarium oxysporum sesame

The invention relates to an identification method of pathogenicity of fusarium oxysporum sesame special type bacterial strain to host sesame, which comprises the following steps: (1) pretreatment of seeds; (2) bacterial inoculation culture of the seeds: conidium suspending liquid is taken and poured into sterile vermiculite with the volume to be two times that of the conidium suspending liquid, vermiculite mixed with pathogenic bacteria and sterile soil are mixed evenly according to the volume ratio of 1:3 and charged in a split mode, sterile sesame seeds exposing white are sown in a paper bowl, 10 particles are evenly placed in each bowl, each variety is repeated and planted for three times, mixed soil for mixing bacteria is lightly covered on the surface of the seeds and placed into an artificial climate box for light and dark alternated culture for 12 hours per day at 25 DEG C and at the humidity of about 80%, and other culture conditions are the same with equal-volume sterile water as comparison; and (3) performing pathogenicity identification. The identification method is simple, convenient and easy to implement, pathogenicity of the pathogenic bacteria is stable, results are reliable, repeatability is good, and the fact that whether fusarium oxysporum sesame special type bacterial strain has pathogenicity on sesame seedlings and pathogenic strength of the fusarium oxysporum sesame special type bacterial strain can be accurately reflected.
Owner:HENAN ACAD OF AGRI SCI

Small internal combustion engine crankshaft system simulation device and simulation method thereof

The invention discloses a small internal combustion engine crankshaft system simulation device and a simulation method thereof. The device comprises a mounting platform, a servo motor, a driving crankshaft, a left connecting rod, a double-end cylinder, a right connecting rod, a driven crankshaft and a flywheel, the servo motor and the double-head air cylinder are both mounted on the mounting platform; two ends of a main journal of the driving crankshaft are mounted on the mounting platform through a bearing and a bearing seat; a main journal of the driving crankshaft and an output shaft of the servo motor are coaxially connected and can rotate synchronously; a connecting rod neck of the driving crankshaft is hinged with one end of a left connecting rod; the other end of the left connecting rod is hinged with a main piston rod of the double-head cylinder; two ends of a main journal of the driven crankshaft are mounted on the mounting platform through a bearing and the bearing seat; the main journal of the driven crankshaft and the flywheel are coaxially connected and can rotate synchronously; a connecting rod neck of the driven crankshaft is hinged with one end of a right connecting rod; and the other end of the right connecting rod is hinged with a slave piston rod of the double-head cylinder. The crankshaft system of the internal combustion engine can be truly simulated, and the equivalence requirement is met.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY

Climate simulation system for researching storage stability of liquid petroleum product

The invention discloses a climate simulation system for researching the storage stability of a liquid petroleum product. The system comprises a temperature adjusting device, a humidity adjusting device, a fresh air device, an air circulating device and a salt spray adjusting device; the temperature adjusting device which comprises an air-cooled single-stage double-compressor refrigerating unit and a stainless steel electrothermal tube is used for adjusting the temperature of the storage environment of the liquid petroleum product; the humidity adjusting device comprises a relational expression humidifier; and the salt spray adjusting device comprises a salt solution tuner and a high pressure sprayer; a heat exchanger is used for exchanging heat of air entering the storage environment of the liquid petroleum product with heat discharged from the storage environment of the liquid petroleum product; and the air circulating device is used for circulating air in the storage environment of the liquid petroleum product. By adopting the system disclosed in the invention, the storage environment of the liquid petroleum product can be adjusted to make the environment of the liquid petroleum product in a laboratory coincide with the practical case outside the laboratory, thereby the research result can be accurate.
Owner:PETRO OIL & LUBRICANT RES INST OF THE GENERAL LOGISITIC DEPT OF PLA +1

A small internal combustion engine crankshaft system simulation device and simulation method

The invention discloses a small internal combustion engine crankshaft system simulation device and a simulation method thereof. The device comprises: an installation platform, a servo motor, a driving crankshaft, a left connecting rod, a double-headed cylinder, a right connecting rod, a driven crankshaft and a flywheel; a servo motor Both ends of the main journal of the driving crankshaft are installed on the installation platform through bearings and bearing seats; the main journal of the driving crankshaft is coaxially connected with the output shaft of the servo motor, and the two can rotate synchronously; The connecting rod journal of the driving crankshaft is hinged with one end of the left connecting rod; the other end of the left connecting rod is hinged with the main piston rod of the double-headed cylinder; the two ends of the main journal of the driven crankshaft are installed on the installation platform through bearings and bearing seats; The main journal of the driven crankshaft is coaxially connected with the flywheel, and the two can rotate synchronously; the connecting rod journal of the driven crankshaft is hinged with one end of the right connecting rod; the other end of the right connecting rod is hinged with the slave piston rod of the double-headed cylinder; the present invention It can truly simulate the crankshaft system of an internal combustion engine and meet the requirements of equivalence.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY

Method for obtaining low-rank coal pyrolysis characteristic parameters and semi-coke combustion characteristic parameters

The invention discloses a low-rank coal pyrolysis characteristic parameter and semicoke combustion characteristic parameter obtaining method comprising the following steps: A, grinding and screening the low-rank coal so as to obtain coal powders; B, adding the coal powders into a thermobalance crucible, and replacing the atmosphere in the thermobalance into inert atmosphere under room temperature; C, under the inert atmosphere protection, heating the coal powders up to a preset temperature in a preset heating rate, carrying out pyrolyzation so as to obtain coal semicoke, and obtaining a coal powder weight time change curve and a temperature time change curve; D, regulating the temperature in the thermobalance to the semicoke combustion temperature, and insulating for certain time; E, switching inert atmosphere into semicoke combustion atmosphere, carrying out semicoke combustion under insulation state, thus obtaining coal semicoke weight time change curve and a temperature time change curve. The parameter obtaining method is simple in process, can eliminate influences on semicoke preparation by different pyrolysis devices, can use a static method to study low-rank coal semicoke combustion features, thus obtaining more accurate parameters.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI

Method for researching maximum load average value of resident living electricity users

The invention discloses a method for researching the maximum load average value of resident living electricity users. The method comprises the steps of selecting users in different areas, selecting measurement objects, determining the sampling time, drawing a user electricity broken line graph, determining an electricity load maximum value and calculating an average value. The beneficial effects of the invention are that: t the method for researching the maximum load average value of the resident living electricity users is reasonable in design; the selected residential areas are divided intothree categories of urban residential users, suburb residential users and rural residential users according to living positions; therefore, the selected cell sample is more representative; the research result is more accurate; the user residence division types are divided according to residence rooms or residence areas; sampling time is set at an interval of 10 minutes, and data such as the current, the voltage, the active power, power factor values and the like of each measuring point are automatically recorded by using a tester, so that the base number of the acquired data is large enough, and a research result is more accurate when maximum load average value research is performed.
Owner:湖南迪泰尔综合能源规划设计有限公司

Gel composition, patch and application thereof

ActiveCN109999205BSolve the problem of unstable patch results of antagonistic stimuliReduce misjudgment of resultsCompounds screening/testingFilm-forming agentBiochemistry
The invention discloses a gel composition, patch and application thereof. The gel composition of the present invention includes a film former, a thickener, a penetration enhancer and a solvent. The use of the gel composition in patch products can solve the problem of unstable results of antagonistic stimulus patches and reduce misjudgment of results caused by unstable stimulus modeling.
Owner:三立慧评(北京)检测技术有限公司

Culture method for plant seedlings

The invention relates to a culture method for plant seedlings. The plant seedlings are cultured by a ventilated root division culture device, the side surfaces of two culture bowls of the device are in butt joint, a cover plate and a culture plate are arranged on the two culture bowls, culture holes are formed in the culture plate, and vent holes are formed in the two sides of the culture holes. The culture method comprises the followings steps: the plant seedlings of 3 days are taken and cultured in a Hoagland's nutrient solution with the concentration of 50 percent for 2 days then in a Hoagland's nutrient solution with the concentration of 100 percent for 12 days, the plant seedlings growing consistently are taken, the stems of the plant seedlings are wrapped by nutrient cotton and fixed in the culture holes of the culture plate, the roots of the plant seedlings are divided into a group A and a group B, the group A is put into the culture bowl containing the Hoagland's nutrient solution, and the group B is put into the culture bowl containing a nutritional deficiency nutrient solution; the pH values of the nutrient solutions are regulated to 6 at 8 o'clock in the morning and 15 o'clock in the afternoon; the nutrient solutions are changed every other three days; and after 4-12 days, the plant seedlings are taken out. The operation is simple, and the personal errors are reduced, so that the research result is more accurate, the culture time is saved, and the working efficiency is improved.
Owner:HENAN UNIV OF SCI & TECH
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