36results about How to "Research results are accurate" patented technology

The invention relates to a culture method for plant seedlings. The plant seedlings are cultured by a ventilated root division culture device, the side surfaces of two culture bowls of the device are in butt joint, a cover plate and a culture plate are arranged on the two culture bowls, culture holes are formed in the culture plate, and vent holes are formed in the two sides of the culture holes. The culture method comprises the followings steps: the plant seedlings of 3 days are taken and cultured in a Hoagland's nutrient solution with the concentration of 50 percent for 2 days then in a Hoagland's nutrient solution with the concentration of 100 percent for 12 days, the plant seedlings growing consistently are taken, the stems of the plant seedlings are wrapped by nutrient cotton and fixed in the culture holes of the culture plate, the roots of the plant seedlings are divided into a group A and a group B, the group A is put into the culture bowl containing the Hoagland's nutrient solution, and the group B is put into the culture bowl containing a nutritional deficiency nutrient solution; the pH values of the nutrient solutions are regulated to 6 at 8 o'clock in the morning and 15 o'clock in the afternoon; the nutrient solutions are changed every other three days; and after 4-12 days, the plant seedlings are taken out. The operation is simple, and the personal errors are reduced, so that the research result is more accurate, the culture time is saved, and the working efficiency is improved.

The invention relates to a system and a method for realizing synchronous control of multiple targets in a detonation experiment test system. The system comprises a shock tube, a premixed gas supply system, a flame retardant injection system, a flame signal acquisition system, a pressure signal acquisition system, a data acquisition system, a laser schlieren system, a high-pressure ignition systemand a synchronous control system for controlling the systems, the shock tube comprises a plurality of experiment pipelines and a visual observation window experiment section which are connected in sequence; the premixed gas supply system is communicated with the interior of the shock tube to generate premixed gas with a preset equivalence ratio and input the premixed gas into the shock tube; the flame retardant spraying system is connected with the visual observation window experiment section; the flame signal acquisition system and the pressure signal acquisition system are arranged on the side wall of each section of experiment pipeline; the laser schlieren system is used for measuring distribution images of typical free radical concentration and temperature of the deflagration flow field; the high-pressure ignition system is connected with the end of the shock tube and used for ignition. The system and the method can be widely applied to the field of safety science and technology.

The invention discloses a rocker arm type valve wear test device which comprises a rocker arm platform, a rocker arm, a cam, a motor, a combustion chamber, a valve tapered head, a valve, a valve retainer and an impact spring for driving the valve to impact the valve retainer. A valve head of the valve stretches into the combustion chamber, the valve retainer is arranged in the combustion chamber, a valve rod of the valve stretches out of the combustion chamber, the valve tapered head is fixedly connected to the valve rod, the valve rod is sleeved with the impact spring, the two ends of the impact spring are connected with the valve tapered head and the combustion chamber respectively, the middle of the rocker arm is hinged to the rocker arm platform, one end of the rocker arm is the driven end driven by the cam, the other end of the rocker arm is a trigger end for driving the valve rod to move, and the motor is used for driving the cam to rotate. The device is driven by the motor, a roller makes contact with the cam, wear between the cam and the rocker arm is reduced, contact of the rocker arm and the valve is more accurate, and the wear condition produced when the rocker arm makes contact with the valve can be accurately observed.

The invention relates to a method for designing, amplifying and sequencing twelve pairs of floccularia luteovirens microsatellite primers. The method comprises the following steps: (1) extracting genome DNA of three floccularia luteovirens populations among which the geographic interval is more than 300km by using an improved CTAB method; (2) randomly selecting an individual genome DNA respectively from the three populations, mixing, detecting the quality of total DNA, preparing a gene library, and performing Illumina HiSeq<TM>2500 sequencing after the gene library is qualified in examination in depot; (3) splicing sequenced data, detecting simple sequence repeats (SSR) in the total DNA sequence by using SR search software, and performing primer design by applying primer3; (4) preparing an SSR primer having an annealing temperature of 50-60 DEG C by adopting a temperature gradient method; (5) respectively performing PCR amplification on the genome DNA of the three floccularia luteovirens populations, and sequencing and verifying to obtain 12 pairs of primers with polymorphism; and (6) calculating the number N of allelic genes, haplotype diversity H<d> genetic differentiation coefficient F<ST>, nucleotide diversity P, G<ST> and the value of pi. The method is beneficial to large-scale research.