41 results about "Disease mechanisms" patented technology

An analytic apparatus and method is provided for diagnosis, prognosis and biomarker discovery using transcriptome data such as mRNA expression levels from microarrays, proteomic data, and metabolomic data. The invention provides for model-based analysis, especially using kernel-based models, and more particularly similarity-based models. Model-derived residuals advantageously provide a unique new tool for insights into disease mechanisms. Localization of models provides for improved model efficacy. The invention is capable of extracting useful information heretofore unavailable by other methods, relating to dynamics in cellular gene regulation, regulatory networks, biological pathways and metabolism.

The present invention relates to identification and uses of biomarkers for neurodegenerative disease, including Alzheimer's disease, and the related diseases. More specifically, the present invention relates to the identification of protein biomarkers useful for the screening, diagnosis, and differentiation of Alzheimer's disease from Parkinson's disease, other neurodegenerative diseases, and normal controls, and in the monitoring of Alzheimer's disease severity and disease mechanisms in patients.

The present invention is a synthetic microfluidic microvasculature network and associated methods. The synthetic microfluidic microvasculature network mimics the structure, fluid flow characteristics, and physiological behavior of physiological microvasculature networks. Computational methods for simulating flow and particle adherence in synthetic and physiological microvascular systems and methods for determining parameters influencing particle adhesion and drug delivery are also described. The invention has many uses including the optimization of drug delivery and microvascular treatments and in describing disease mechanisms that affect the microvasculature such as inflammation, diabetes and hypertension.

The invention discloses an efficient amplifying and culturing method for biliary epithelial cells in rabbit livers and belongs to the technical field of biological medicines. The efficient amplifying and culturing method comprises the following steps of: (1) separating, purifying and culturing the epithelial cells; (2) detecting cell growth lines; and (3) identifying the epithelial cells. The efficient amplifying and culturing method for the biliary epithelial cells, disclosed by the invention, has the advantages as follows: through a multi-step separating method, biliary epithelial cells with higher purities are obtained and a long-time multiplication culturing system for the biliary epithelial cells is established; a fact that the multiplication of the biliary epithelial cells in the livers can be obviously promoted through an Rho kinase inhibitor Y-27632 is first found; and the efficient amplifying and culturing method for the biliary epithelial cells, disclosed by the invention, has the advantages of simpler operation and the like, is suitable for promotion and application and can provide a platform for researches on a plurality of disease mechanisms including biliary atresia, primary biliary cirrhosis and the like.

The invention discloses a kit for detecting child active tuberculosis and an application of the kit. The kit is combined with a bioinformatics method to screen characteristic metabolic products of child active tuberculosis, and a detecting method for the child active tuberculosis is established based on chemical shift of the characteristic metabolic products of child active tuberculosis; experiments show that in verification of 24 samples by the detecting method provided by the invention, the accuracy is 100%, the specificity is 100% and the sensitivity is 100%; in addition, the detecting method is simple and easy to operate and can be used for early detection of child active tuberculosis; the detecting method provides a new approach for clinical diagnosis of child active tuberculosis as well as provides new thoughts for exploring disease mechanisms.

The invention discloses a method for researching differentially expressed proteins of triple negative breast cancer exosomes by using an iTRAQ technology. The method comprises the following steps of (1) extracting and treating exosomes and exosome proteins; (2) carrying out enzymatic hydrolysis and desalination on exosome protein samples obtained in the step (1) to obtain polypeptides; (3) carrying out iTRAQ labelling and mixing on the polypeptides obtained in the step (2); (4) carrying out HPLC separation and LC-MS analysis on a polypeptide-peptide mixture labelled in the step (3); and (5) carrying out bioinformatics analysis and quantitative analysis on the obtained mass spectrometry identification result to obtain the differentially expressed proteins of the triple negative breast cancer exosomes. Research on triple negative breast cancer exosome proteome can be quickly and effectively carried out by combining the iTRAQ technology disclosed by the invention with an MS/MS technology,and a reference is provided for researching a disease mechanism of a triple negative breast cancer and discovering triple negative breast cancer-related biomarkers.

This invention relates to the advanced material field of the quantum information, it relates to the self assembling bi-stable quantum line array and its production method of nanometer medicine concretely. The invention self assembles antioxidase oxygen free-radical antagonist by the interaction of the inelasticity electron tunnel penetration, the beta-acceptor excitant, the P2 acceptor excitant, the benzene alkane amine calcium antagonist / or purine nucleic acid monomer and their binary , three-element, four -element and five -element compound. The invention quantum line array have regular geometry configuration, the dimension is controllable and adjustable, the inelasticity electron tunnel penetration, the quantum bit electricity characteristic and the Kondo effect. The invention not only benefits the study of the innovation medicine of target disease form, but also benefits the study of quantum bio-information sensor new material and nanometer structure magnetism memory (MRAM).

The invention provides a micro-fluidic chip which comprises a channel layer and a substrate layer; the channel layer comprises a sample introduction area, a mixing area and a detection area which are sequentially communicated; the mixing area comprises a plurality of mixing units; each mixing unit comprises at least one wide channel and at least one narrow channel, wherein the width of the wide channel is 100-300 microns, and the width of the narrow channel is 1/5-1/2 of the width of the wide channel; and target samples can be efficiently mixed, and functional areas including mixing, separation and detection are integrated on the chip at the same time, so that the requirements of reaction, separation, detection and the like can be met, and the production or research efficiency can be effectively improved. The obtained micro-fluidic chip can also be applied to the field of plasma lipoprotein and exosome separation, and is beneficial to promoting the application of lipoprotein and exosome in disease mechanism research and diagnosis.

Provided herein are methods for assessing a disease score of a subject suffering from or suspected to be suffering from chronic obstructive pulmonary disease (COPD) or associated disease mechanisms, wherein the disease score represents COPD activity. The disease score can be used to stratify the subject into a specific risk category and can further inform patient management decisions. The methods can involve determining a biomarker signature including four or more biomarkers associated with COPD or COPD mechanisms. The methods can further include supplementing the biomarker combinations with calculation or classification trees based on one or more additional clinical parameters or biomarkers. In some cases, the methods include timing of collection of patient samples with respect to acute event or treatment course.

The invention discloses a metabonomics pathway-based myocardial infarction early warning method. The method specifically comprises the following steps of S1, taking in 10 patients with qi deficiency and easy chest distress and urgent breath and 10 healthy and symptomatic healthy people, not intervening in the normal life of the healthy people, and taking salvia miltiorrhiza traditional Chinese medicine formula granules for the patients with qi deficiency; and S2, respectively carrying out morning fasting blood sample collection on the two groups of subjects before and after intervening, settling the collected blood for 1.5 hours, centrifuging for 10 minutes at the temperature of 5 DEG C, and sealing after centrifuging, so that the invention relates to the technical field of medicines. According to the metabonomics pathway-based myocardial infarction early warning method, the nuclear magnetic resonance technology is combined with multivariate statistics to analyze the content change ofserum potential markers related to myocardial infarction resistance of salvia miltiorrhiza, and then metabolic pathway analysis is performed on the potential markers, so early warning of myocardial infarction of a patient is facilitated; and research of medical researchers on disease mechanisms is facilitated.

The invention provides a novel efficient mycoplasma columbinum culture medium FG1. The culture medium is obtained through adding dove serum, and polypeptide T1 as shown in a SEQ ID: 1 sequence based on a conventional culture medium FM-4 of mycoplasma. The mycoplasma columbinum can realize rapid growth and proliferation in FG1 culture mediums, and can achieve very high viable bacteria titer, so that rapid detection and diagnosis of the mycoplasma columbinum are facilitated, rapid separation and resolution of the mycoplasma columbinum are facilitated, the development of mycoplasma columbinum vaccines and treatment medicines is facilitated, and research of disease mechanisms of the mycoplasma columbinum is facilitated.

The microfluidic platform device of the claimed invention makes use of a high throughput lab-on-a-chip format to determine the functional profile of antibodies elicited by infection or vaccination against infection for any pathogen. It can be used to evaluate vaccines, evaluate whether vaccinated individuals show indices of protection, determine whether individuals display resistance or susceptibility to infection, discover new vaccine antigens, discover and test therapeutic interventions, or evaluate mechanisms of disease.

The invention discloses a method for key RNA function mining based on high-throughput experimental data mining. By integrating multiple data to obtain concomitant data sets and combining the concomitant data sets with clinical data sets, the most relevant genes for project research can be identified from a large set of known RNAs, while the function of unknown RNAs can be predicted to better determine the roles the unknown RNAs exercise in life activities, so that an important basis is provided for subsequent aspects of disease mechanisms, drug targets, disease diagnosis and the like.

The invention discloses a pluripotent stem cell establishment and stemness maintenance method. The method comprises the following steps: 1, selecting proper maternal cells; 2, extracting maternal cells, culturing the cells, and selecting healthy maternal cells; 3, treating the maternal cells to obtain novel pluripotent stem cells. According to the invention, the bHLH transcription factors with different concentrations are adopted to inhibit the differentiation condition of the pluripotent stem cells, and the bHLH transcription factors with different concentration gradients are set to explore the pluripotent stem cells, so the influence condition of the bHLH transcription factors with specific concentrations on the differentiation of the stem cells can be confirmed; in this way, the pluripotent stem cells can be placed in the bHLH transcription factor culture solution with specific concentration, and the stemness of the pluripotent stem cells can be maintained, so the pluripotent stem cells can be suitable for subsequent biological or medical research on disease mechanisms, model preparation, chromosome engineering, genome stability and the like.