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37 results about "In situ visualization" patented technology

Method and device for determining flow characteristic of carbon dioxide in porous medium and distribution rule of methane in pore space

The invention relates to a method and a device for determining the flow characteristic of CO2 in a porous medium and the distribution rule of CH4 in a pore space. The method comprises the following steps: (1) pretreating a sample; (2) system connection and air tightness inspection; (3) vacuumizing the system; (4) temperature control; (5) CH4 saturation and pressure stabilization; (6) displacement experiment and data acquisition; (7) monitoring outlet gas; and (8) data processing. The problem that in existing CO2 geological sequestration and enhanced gas production research, due to the fact that in-situ visualization of the pore size cannot be achieved, a microscopic displacement mechanism is unclear is solved, and the method aims at providing scientific basis and technical support for efficient exploitation of natural gas and effective geological sequestration of CO2.
Owner:SANYA MARINE OIL & GAS RESEARCH INSTITUTE NORTHEAST PETROLEUM UNIVERSITY

Virus three-dimensional reconstruction method and system based on three-dimensional Gaussian splashing

The embodiment of the invention discloses a virus three-dimensional reconstruction method and system based on three-dimensional Gaussian splashing. The method comprises the following steps: acquiring three-dimensional point cloud data including a target virus; performing molecular dynamics simulation on the target virus based on a coarse graining molecular dynamics model, identifying a high-energy interaction region, and obtaining binding energy data of the target virus and a host molecule in an interaction process; tracing the conformation change of the virus structure to obtain conformation change data; performing three-dimensional Gaussian splashing processing on the point cloud data to generate an in-situ visual three-dimensional model of the target virus; wherein in the Gaussian splashing processing, the shape, the direction and the weight of a Gaussian kernel are dynamically adjusted according to combined energy data and conformation change data. According to the method and the device, efficient virus three-dimensional model generation is realized aiming at virus characteristics.
Owner:WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI

Research method for mechanism of extracting elemental gold in hydrothermal fluid from crude oil

The invention belongs to the technical field of petroleum and natural gas mineral geochemical experiment research, and discloses a research method for a mechanism of extracting elemental gold in hydrothermal fluid from crude oil. In-situ visualization experiments are carried out in a transparent fused quartz capillary tube, and analysis is carried out in combination with a scanning electron microscope, an energy dispersion X-ray spectrum and a transmission electron microscope, so that continuous evolution of water droplets near an interface of oil and a solution containing HAuCl4 at a high temperature is successfully proved. According to the method disclosed by the invention, hydration electrons released in a conversion process from OH <-> to OH < * > in the water droplets are extracted, the hydration electrons and H2O2 formed in the droplets have a synergistic effect, Au3 < + > is promoted to be reduced to form natural gold atoms, the atoms are gathered into nano-scale and micron-scale gold particles in a droplet coalescence process, and a clear and reasonable mechanism is provided for formation of elemental gold in crude oil extraction hydrothermal fluid.
Owner:CHINA UNIV OF PETROLEUM (EAST CHINA)

Guniting thickness in-situ visualization method and system based on mixed reality

The invention provides a mixed reality-based guniting thickness in-situ visualization method and system, relates to the field of civil engineering and intelligent construction, and aims to realize in-situ visualization of guniting thickness detection by using a mixed reality technology. The method comprises the steps of constructing a three-dimensional grid representation of a guniting operation site, and constructing a three-dimensional grid representation of the guniting operation site by using a space anchor point technology; a virtual mechanical arm corresponding to a physical mechanical arm in the guniting operation site is established, and the virtual mechanical arm and the physical mechanical arm are kept consistent in pose and spatial position; generating virtual guniting particles, and setting particle parameters for the virtual guniting particles; when it is detected that the virtual guniting particles collide with the environment grid every time, the three-dimensional coordinates of collision points are mapped to grid units in the two-dimensional height field grid, and the current deposition thickness value of the grid units is calculated; and based on the deposition thickness value of the grid unit, obtaining the spraying thickness of each position point in the grid unit, and rendering the spraying thickness of each position point to the target surface through a corresponding color.
Owner:BEIJING UNIV OF TECH

All-solid-state battery thin-layer electrode device for multi-mode in-situ interface research and application of all-solid-state battery thin-layer electrode device

The invention relates to an all-solid-state battery thin-layer electrode device for multi-mode in-situ interface research. The all-solid-state battery thin-layer electrode device comprises the following components: a solid electrolyte substrate; the functional thin layer is arranged on the surface of the solid electrolyte substrate; the functional thin layer has conductivity; the functionalized thin layer allows a detection signal of an in-situ characterization technology to effectively penetrate or be reflected from the surface of the functionalized thin layer; the in-situ characterization techniques include atomic force microscopes, optical microscopes, and Raman spectroscopy, X-ray photoelectron spectroscopy, infrared spectroscopy, or X-ray diffraction. The invention provides a universal thin-layer electrode design and system and a thin-layer electrode in-situ imaging and spectroscopic characterization method for all-solid-state battery interface reaction. The objective of the invention is to expose a solid electrolyte-electrode interface reaction process which is difficult to observe in an effective detection range of various characterization technologies so as to realize real-time and in-situ visualization and accurate analysis of a dynamic evolution process of an internal interface of an all-solid-state battery.
Owner:INST OF CHEM CHINESE ACAD OF SCI

In-situ derivatization mass spectrometry imaging method

The invention discloses an in-situ derivatization mass spectrometry imaging method, which belongs to the technical field of mass spectrometry imaging detection, and comprises the following steps: preparing a sample slice and attaching the sample slice to a conductive glass slide; the method comprises the following steps: dissolving a derivatization reagent and a catalyst in a volatile organic solvent to prepare a derivatization reagent system solution, and spraying the derivatization reagent system solution on the surface of the tissue slice for derivatization reaction; coating a matrix on the surface of the derivatization slice, and identifying and analyzing by using an MALDI-MSI method; the structure of the derivatization reagent is shown in the specification. The derivatization reagent can perform mild and rapid in-situ derivatization on trace carbonyl-containing compounds in biological tissues, significantly improve the mass spectrum signal response of target compound molecules and significantly inhibit the delocalization (diffusion) phenomenon of analytes, and the MALDI-MSI technology is adopted to analyze the derivatized tissue slices. High-spatial-resolution in-situ visualization characterization of trace carbonyl-containing compounds in biological tissue slices is realized, and the problems in the prior art are effectively solved.
Owner:LANZHOU UNIV

Device and method for observing and actively strengthening multi-field coupling damage of recycled concrete interface

The present application relates to the technical field of road engineering test, in particular to a recycled concrete interface multi-field coupling damage observation and active reinforcement device and method, and constructs a closed-loop experimental system of "observation-diagnosis-treatment" integration. The system simulates the real environment, diagnoses the state change of the internal interface area of the test piece in real time by using various high-precision sensors during the stress damage process of the test piece, and treats the weak interface at the key moment. The mechanical loading, environment simulation, in-situ microscopy, multi-parameter monitoring and active reinforcement intervention are integrated, and the whole chain and integrated research from macro performance to micro mechanism is realized. The dynamic, in-situ and visualized research on the most core scientific problem of recycled concrete is realized. Based on the trigger mechanism of multi-source information fusion, the timing of reinforcement intervention is more scientific and accurate, and data support is provided for optimizing the repair strategy.
Owner:HENAN UNIV OF SCI & TECH

Gas-water-rock reaction and particle corrosion-migration in-situ visual quantitative determination method and device based on micro-fluidic chip

The invention belongs to the technical field of unconventional oil and gas development, and particularly relates to a gas-water-rock reaction and particle dissolution-migration in-situ visual quantitative determination method and device based on a micro-fluidic chip. The quantitative determination method comprises the following steps: firstly, designing a chip model containing a real rock core, wherein factors such as a strong heterogeneous structure of the compact conglomerate oil reservoir and strong difference of a pore throat structure are considered at the same time; then, the gas-water-rock reaction and the particle dissolution-migration process are recorded through an optical camera; and finally, analyzing contribution degrees of different minerals to a gas-water-rock reaction and judging different corrosion modes of the conglomerate by combining image processing software and a mathematical model. The method can be used for carrying out in-situ visual quantitative determination on gas-water-rock three-phase reaction, particle corrosion and a secondary migration process in the conglomerate reservoir under the conditions of oil reservoir temperature pressure and fluid chemistry.
Owner:CHINA UNIV OF PETROLEUM (EAST CHINA)

An in-situ visualization rapid measurement method for measuring the gas dissolution rate

The present invention discloses an in-situ visualization rapid measurement method for measuring the gas dissolution rate: horizontally place the test substrate into the box body; turn on the planar light source assembly and focus it on the microcavity of the test substrate; evacuate the inside of the highly transparent heat-resistant glass main box, and inject the test gas into the highly transparent heat-resistant glass main box through the gas injection assembly; inject the test liquid into the internal liquid box through the liquid injection assembly until the microcavity at the bottom of the test substrate is quickly flooded and bubbles are generated inside the microcavity; use a CCD camera and a data acquisition and recording system to observe and record in real time the generation and dissolution disappearance process of the bubbles inside the microcavity of the test substrate, record the time and images of the whole process, and calculate the dissolution rate of the gas in the liquid medium. In the case of a large machine vision recording range, the present invention realizes the measurement and monitoring of the air dissolution rate inside multiple microcavities through one measurement, obtains a large number of samples, and the obtained average result is accurate, avoiding the contingency of the experiment.
Owner:INST OF MECHANICS CHINESE ACAD OF SCI

Carbon nano chemical indicator, preparation method thereof and application of carbon nano chemical indicator in bacterial proliferation monitoring

The invention discloses a carbon nano chemical indicator, a preparation method thereof and application of the carbon nano chemical indicator in bacterial proliferation monitoring. The method comprises the following steps: S1, preparing nitrogen-sulfur co-doped carbon quantum dots; and S2, carrying out surface modification by adopting 1, 2-naphthoquinone-4-sodium sulfonate, so as to obtain the carbon nano chemical indicator. The intelligent response type chemical indicator based on the carbon quantum dots is innovatively developed, according to the scheme, a specific response system is constructed through surface modification of the carbon quantum dots, and real-time and in-situ visual monitoring of bacterial proliferation is achieved through fluorescence / color dual-mode changes triggered by a bacterial metabolism microenvironment; compared with a traditional method, the method has the outstanding advantages that the response is fast (lt; the method can realize dynamic monitoring, does not need complex pretreatment, supports nondestructive detection, reduces the detection cost by more than 80%, has double functions of naked eye visualization and instrument quantitative detection, and is suitable for universal detection of a plurality of common bacteria.
Owner:SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI

In-situ visual fluorescence detection method for micro-damage of lead-based perovskite solar cell

The invention discloses an in-situ visual fluorescence detection method for micro-damage of a lead-based perovskite solar cell, and relates to the technical field of perovskite photovoltaic detection. The method comprises the following steps: uniformly dispersing methacrylic acid 3-(trimethoxysilyl) propyl ester, an initiator and meso-tetra (4-carboxyphenyl) porphyrin in a water solvent in a nitrogen atmosphere to obtain a mixture; and treating the mixture at 70-85 DEG C for 20-40 minutes to synthesize gel, so as to obtain the fluorescent probe Si (at) MTCP. According to the invention, synthesized Si-coated MTCP is spin-coated on a cell at a rotating speed of 1000 rpm, and then the cell is heated at 60 DEG C for 2 minutes to form and realize perovskite cell fluorescent probe deposition. The lead leakage in the damaged device can weaken and even quench the fluorescence of the MTCP, and the lead leakage position can be accurate through the phenomenon.
Owner:NORTHWESTERN POLYTECHNICAL UNIV +1

Ph-reversible leuco-BODIPY probe for in situ visualization of extracellular vesicles and their secretion from living cells

ActiveUS12578273B2Methine/polymethine dyesAzo dyesIntracellular vesicleExtracellular vesicle
The subject invention pertains to compositions of a probe comprising two pH-reversible forms, including the boron dipyrromethene (BODIPY) fluorescent form and the non-fluorescent leuco-BODIPY form, for use in confocal imaging methods of intracellular vesicles as well as extracellular vesicles that are present in conditioned culture medium and / or secreted by living cells, without inducing severe cytotoxicity.
Owner:THE CHINESE UNIVERSITY OF HONG KONG

Method for measuring film-forming coverage rate and shear strength of low-speed heavy-load friction interface lubricating grease deposited film based on fluorescence method

The invention discloses a method for measuring the film-forming coverage rate and the shear strength of a low-speed heavy-load friction interface lubricating grease deposited film based on a fluorescence method, and belongs to the technical field of lubricating grease measurement. The method comprises the following steps: vertically arranging a coaxial illumination fluorescence microscope right above a ball-disc contact area formed by a steel ball and a sapphire disc; a lubricating grease sample dyed by a fluorescent agent is preset in the ball disc contact area; after the excitation light is filtered, the excitation light is horizontally emitted into a coaxial illumination fluorescence microscope, is reflected by a dichroscope, and is vertically irradiated to a lubricating grease sample in a ball disc contact area; a fluorescence signal sequentially passes through a dichroscope and a cut-off filter, is focused and amplified by a coaxial illumination fluorescence microscope, and is captured by a camera; and converting the fluorescence signal into a fluorescence image, and carrying out morphological and gray brightness analysis to obtain the distribution form and aggregation state of the lubricating grease thickening agent in the contact area and in the peripheral friction raceway of the contact area. According to the invention, in-situ, visual and quantitative determination of the film-forming coverage rate and the shear strength of the deposited film of the lubricating grease thickening agent is realized.
Owner:LANZHOU INSTITUTE OF CHEMICAL PHYSICS CHINESE ACADEMY OF SCIENCES

Method for researching MOF (Metal Organic Framework) synthesis process by using low-field nuclear magnetic imaging device

The invention discloses a method for researching an MOF (Metal Organic Framework) synthesis process by utilizing a low-field nuclear magnetic imaging device, and belongs to the technical field of material synthesis process analysis. The method comprises the following steps: firstly, preparing a metal source solution and an organic ligand solution; one of the solutions is selected for low-field nuclear magnetic resonance relaxation time measurement, and imaging sequence parameters are optimized; mixing the two solutions, and immediately putting the mixed solution into a low-field nuclear magnetic imaging device; and carrying out continuous relaxation time weighted imaging and layered relaxation time measurement on the reaction process by using the optimized imaging sequence. The lossless, in-situ, visual and quantitative monitoring of the metal-organic framework material in the nucleation, growth and sedimentation process is realized by analyzing the change of image signal intensity in time and space and the change trend of relaxation time (such as T2 value) of different liquid layers along with time. According to the method, required equipment is simple, operation is convenient and fast, and an innovative research means is provided for deeply understanding the MOF synthesis mechanism and optimizing the synthesis process.
Owner:INSTITUTE OF PROCESS ENGINEERING CHINESE ACADEMY OF SCIENCES

Three-dimensional reconstruction method and system for viruses based on three-dimensional gaussian blurring

The embodiment of the application discloses a virus three-dimensional reconstruction method and system based on three-dimensional Gaussian splash, and the method comprises the following steps: acquiring three-dimensional point cloud data of a target virus; performing molecular dynamics simulation on the target virus based on a coarse-grained molecular dynamics model, identifying a high-energy interaction region, and acquiring binding energy data of the target virus and host molecules in the interaction process; tracking the conformational change of the virus structure to obtain conformational change data; performing three-dimensional Gaussian splash processing on the point cloud data to generate an in-situ visual three-dimensional model of the target virus; and in the Gaussian splash processing, the shape, direction and weight of the Gaussian kernel are dynamically adjusted according to the binding energy data and the conformational change data. The application realizes efficient generation of a virus three-dimensional model according to the characteristics of the virus.
Owner:WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI

Flow field visualization detection method and device for flow battery

The application discloses a liquid flow battery flow field visualization detection method and device, and the method comprises the following steps: opening a cold fluid valve and starting a flow control pump, so that the cold fluid circulates into the flow field in the visualization pool at a preset rate of the flow control pump until the temperature of the flow field reaches a preset initial temperature; closing the cold fluid valve and opening a hot fluid valve, so that the hot fluid flows into the flow field at a preset rate of the flow control pump, and the hot fluid and the cold fluid remaining in the flow field are subjected to convection heat exchange; acquiring color image data displayed by a temperature-sensitive color-changing liquid crystal sheet in the visualization pool through a camera within a preset time period, the color image data being displayed due to the temperature change of the flow field; acquiring multiple color images corresponding to multiple preset time points in the color image data respectively; calculating uniformity coefficients of preset colors in the multiple color images respectively; and judging the uniformity of the flow field distribution according to the uniformity coefficients. The purpose is to characterize the fluid distribution state in the flow field by monitoring the temperature distribution of the flow field in real time, so as to realize in-situ visualization of the flow field.
Owner:THE CHINESE UNIV OF HONG KONG (SHENZHEN) +1

A fluorescence in situ hybridization method for co-localization of multiple genes in hemocytes of bivalves

The present invention discloses a fluorescence in situ hybridization method for co-localization of multiple genes in shellfish hemocytes, belonging to the technical field of cell biology of marine invertebrates. By optimizing the osmotic pressure buffer system and the preparation method, the present invention can stably obtain a monolayer sample with complete and uniform cell morphology. By advancing the cell morphology imaging step, the complete morphological information of cells is obtained and fixed as a reference before implementing the stringent in situ hybridization process. The present invention can achieve in situ visualization of multiple target genes while better preserving the cell morphological structure, which helps to obtain more accurate and stable spatial distribution information of gene expression at the single-cell level, improves the overall reliability of the experiment, and provides a more effective analysis tool for the study of cell functions of marine invertebrates.
Owner:OCEAN UNIV OF CHINA +1

A method for in situ visualization and biological function research of cell pd-l1 glycosylation based on hybridization chain reaction

PendingCN122445771ADendritic cellDendrite
The application discloses a cell PD-L1 glycosylation in-situ visualization and biological function research method based on hybridization chain reaction. The application adopts a hybridization chain reaction strategy (HCR) of a logic "AND" gate activated by nucleic acid aptamer recognition and metabolic glycan labeling, and constructs a new method for a cell PD-L1 glycosylation in-situ visualization and biological function research method. Through HCR signal amplification induced by adjacent double recognition, the detection sensitivity of PD-L1 glycosylation on different cells is significantly improved, and subtle fluctuations in the PD-L1 glycosylation level can be accurately captured, so as to provide an analysis tool for revealing the dynamic changes under different physiological or pathological conditions. Meanwhile, the application first clarifies the immune regulation function of the PD-L1 glycosylation of dendritic cells, and further expands the research scope of tumor immune checkpoints.
Owner:XIAMEN MEDICAL COLLEGE

Language landscape text grammar error correction method and system based on image recognition

The invention relates to the technical field of computer vision and natural language processing, and particularly discloses a language landscape text grammar error correction method and system based on image recognition, and the method comprises the steps: collecting an image containing a language landscape text through a mobile terminal camera; performing optical character recognition on the image, and extracting a target language text; inputting the text into the grammar error diagnosis model to obtain a diagnosis result containing an error position, an error type and a correction suggestion; and finally, on a screen of the mobile terminal, performing spatial alignment and overlapping display on a diagnosis result and a text region in the image in an augmented reality mode. According to the method, a real-time, in-situ and visual quality checking and learning tool is provided for urban managers, scenic region operators and foreign language learners directly aiming at the grammar and translation quality problems of public place language landscape texts, and the problems that traditional manual inspection efficiency is low, and an online tool cannot be linked with a physical scene for checking are effectively solved.
Owner:SHANDONG PETROCHEMICAL INST

Low-freezing-point infiltration and visualization integrated detection method for improved salinized soil in seasonal frozen region

The invention relates to a low-freezing-point infiltration and visualization integrated detection method for improved saline soil in a seasonal frozen region. Cooperative improvement is realized around three links of low-temperature, constant-temperature and constant-flow liquid supply, in-situ visualization imaging and synchronous control and quantitative identification. The method comprises the following steps: stably supplying a tracing solution with the freezing point lower than 0 DEG C in a range of-5 DEG C to + 5 DEG C; the continuous wetting and infiltration process of the surface layer of the soil sample is kept uninterrupted; a non-destructive in-situ observation window is constructed through fixed geometry and exposure combination of a wedge-shaped or cutting edge transparent observation module, an industrial camera and annular illumination; the central control and data processing module is used for implementing timestamp alignment and closed-loop coordination on pump speed, temperature control, illumination and imaging; according to the system, interface and alignment errors are reduced, energy consumption and arrangement complexity are reduced, repeatability, comparability and efficiency of tests are improved, and a standardized data basis is provided for mechanism research, parameter inversion and model calibration under the condition of an indoor rack / transparent soil column.
Owner:CHANGCHUN INST OF TECH

An in-situ visualization rapid measurement device for measuring the gas dissolution rate

The present invention discloses an in-situ visualization rapid measurement device for measuring the gas dissolution rate. The device includes an observation system arranged at the upper part, a test substrate arranged in the middle, and a planar light source assembly arranged at the lower part; the planar light source assembly provides a visual background illumination condition for the test substrate; the observation system is used to record and save the measurement process in real time, and adjust the focal length and magnification to make the image clearly visible; the test substrate uses microcavities at the micron and millimeter levels as reaction chambers for testing the gas dissolution rate, and is made into a transparent test substrate, with optical photo detection as the main testing method; the volume of a single microcavity in the microcavity sample adopted by the present invention is very small, and the size can be controlled from a few microns to a few centimeters, and it can meet the measurement requirements of different two-phase media. The reaction speed is faster than that of a large-volume reaction chamber, the measurement time is short, and the measurement of the air dissolution rate is more accurate.
Owner:INST OF MECHANICS CHINESE ACAD OF SCI

A microfluidic chip-based gas-water-rock reaction and particle dissolution-migration in-situ visualization and quantitative determination method and device

The present application belongs to the technical field of unconventional oil and gas development, and particularly relates to a gas-water-rock reaction and micro-particle dissolution-migration in-situ visualized quantitative determination method and device based on a micro-fluidic chip. The quantitative determination method designs a chip model containing a real core by simultaneously considering factors such as strong heterogeneity of a dense conglomerate reservoir, strong difference of pore throat structure and the like; then records the gas-water-rock reaction and micro-particle dissolution-migration process through an optical camera; finally, combines image processing software and a mathematical model to analyze the contribution degree of different minerals to the gas-water-rock reaction and to distinguish different dissolution modes of conglomerate. The method can quantitatively determine the gas-water-rock three-phase reaction, micro-particle dissolution and secondary migration process in a conglomerate reservoir under the conditions of reservoir temperature, pressure and fluid chemistry.
Owner:CHINA UNIV OF PETROLEUM (EAST CHINA)

A method and apparatus for laboratory investigation of ultrasonic visualization solutions to near wellbore contamination

The present application relates to a kind of laboratory for studying the method and device for solving near well pollution with ultrasonic visualization.The present application relates to the technical field of oil and gas field development and stimulation, and the device is composed of ultrasonic system, displacement system, visualization monitoring system and data acquisition and control system;Focused ultrasonic transducer is accurately acted on microfluidic core model in cooperation with three-dimensional displacement platform, displacement system contains annular pressure automatic tracking module to simulate formation pressure, and high-speed camera realizes pore scale dynamic observation.The method comprises the following steps: establishing pollution model, applying controllable ultrasonic wave, in-situ visualizing collecting cavitation bubble and pollutant transport process, synchronously collecting seepage data and correlating and analyzing plugging removal mechanism.The present application can real-time observe the microcosmic dynamics process of ultrasonic plugging removal, quantify the relationship between frequency, power and plugging removal efficiency, and is suitable for mechanism research and parameter optimization of various near well pollution types, and solves the "black box" problem that traditional technology cannot in-situ visualize observation.
Owner:NORTHEAST GASOLINEEUM UNIV

Visual detection method and device for flow field of flow battery

The invention discloses a visual detection method and device for a flow field of a flow battery, and the method comprises the steps: opening a cold fluid valve and starting a flow control pump, enabling a cold fluid to circularly flow into a flow field in a visual pool at a preset rate of the flow control pump until the temperature of the flow field reaches a preset initial temperature; the cold fluid valve is closed, the hot fluid valve is opened, the hot fluid flows into the flow field at the speed preset by the flow control pump, and the hot fluid and the cold fluid reserved in the flow field are subjected to convective heat exchange; collecting color image data displayed by the thermochromic liquid crystal sheet in the visual pool along with the temperature change of the flow field in a preset time period through a camera; obtaining a plurality of color images corresponding to a plurality of preset moments in the color image data; respectively calculating uniformity coefficients of preset colors in the plurality of color images; and judging the distribution uniformity of the flow field according to the uniformity coefficient. The method aims to represent the fluid distribution state in a flow field by monitoring the temperature distribution of the flow field in real time, so that the in-situ visualization of the flow field is realized.
Owner:THE CHINESE UNIV OF HONG KONG (SHENZHEN) +1

Separated RUBY visual virus infection system as well as construction method and application thereof

The invention discloses a separated RUBY visual virus infection system as well as a construction method and application thereof. According to the system, a key gene of a betacyanin biosynthetic pathway is split into two parts: CYP76AD1 and a GT gene are connected in series through a 2A peptide sequence and are constructed in a plant stable expression vector, and a stably inherited transgenic plant is obtained in nicotiana benthamiana; and cloning another key gene DODA into a plant virus vector to construct a recombinant virus expression vector. According to the method, real-time, lossless and in-situ visual observation of the virus infection and movement process is achieved, the system construction method is clear, complex instruments are not needed, an efficient and convenient tool platform is provided for protein function analysis, host factor screening and infection mechanism exploration in virology research, and good expandability is achieved.
Owner:GUIZHOU UNIV

Method for evaluating in-situ modified reservoir properties of low to medium mature shale oil

The present application belongs to the technical field of unconventional oil and gas resource development, and specifically discloses a method for evaluating in-situ modification reservoir properties of middle-low mature shale oil, which comprises the following steps: sample selection, diagenetic simulation, thermal simulation sample porosity test, quantitative identification of thermal simulation sample nuclear magnetic analysis pore structure and thermal simulation sample fracture development, final determination of in-situ conversion optimal heating temperature and heating rate parameters, evaluation of shale reservoir in-situ modification effect, and establishment of a quantitative evaluation scheme for reservoir in-situ modification. The method for evaluating in-situ modification reservoir properties of middle-low mature shale oil is fully combined with advanced current testing and in-situ characterization technology, solves the problem of in-situ observation in the evaluation of in-situ conversion shale reservoir of middle-low mature shale oil, effectively solves the problem of non-visualization of the physical process of thermal simulation experiment, realizes the visualized observation of in-situ, and has the advantages of rapidness, non-damage, sensitivity and the like compared with the traditional testing method.
Owner:CHINA UNIV OF PETROLEUM (EAST CHINA)

A benzisoselenazol derivative, its preparation method and application in preparing RNA fluorescent probe

This invention discloses a benzo[selenazole] derivative, its preparation method, and its application in the preparation of RNA fluorescent probes. The benzo[selenazole] derivative provided by this invention has the following structural formula: [structural formula would be inserted here]. This benzo[selenazole] derivative is a typical fluorescently activated probe, exhibiting excellent selective recognition and binding ability for RNA molecules. It demonstrates excellent resistance to biological background interference and exhibits high binding selectivity and recognition specificity for RNA in both live and fixed cell systems. It possesses advantages such as high signal-to-noise ratio and excellent fluorescence photostability, making it suitable for real-time, dynamic fluorescence imaging detection of intracellular RNA. It can achieve in-situ visualization and tracing of changes in subcellular RNA distribution and content. The synthesis method of the benzo[selenazole] derivative provided by this invention has a clear process flow, mild and stable reaction conditions, requires no demanding equipment or special reagents, and uses commercially available and readily available conventional chemicals as raw materials. The operation is simple and easy to perform, with strong overall process operability, making it suitable for large-scale production applications.
Owner:GUANGDONG UNIV OF TECH

In-situ visual detection reagent for cell surface glycosylation RNA (Ribonucleic Acid) and application of in-situ visual detection reagent

The invention discloses an in-situ visual detection reagent for cell surface glycosylation RNA and application thereof, the reagent comprises a glycosylation RNA recognition reagent and a multiple hybrid chain circulation reaction reagent, the glycosylation RNA recognition reagent comprises a nucleotide sequence Tsa of a 5'end modified DBCO functional group, a nucleotide sequence T2 for recognizing RNA and a connection sequence for connecting the Tsa and the T2; the multiple hybrid chain circular reaction reagent is composed of six nucleic acid sequences which are paired with one another. The whole reaction system does not use any enzyme, so that the system is less in interference factors; the method does not use biological enzyme, is isothermal in the whole process, is low in cost of used reagent equipment, is simple to operate, is easy to implement, and is easy to popularize.
Owner:THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV

A three-dimensional visualization and kinetic calculation method for inorganic phase migration in organic-inorganic composite materials

A three-dimensional visualization and kinetic calculation method for the migration of inorganic phases in organic-inorganic composite materials belongs to the technical field of material structure visualization and quantitative analysis. The present invention adopts a fluorescent tracer three-dimensional imaging analysis method to perform an in-situ visualization analysis of the migration process of the inorganic phase in the composite material. By counting the number of nanoparticles at different depths inside the composite material, the migration direction and migration process of the inorganic phase are analyzed. Statistical models are further used to calculate the migration amount, migration speed and direction of the inorganic phase, and the differences in inorganic phase migration under different external conditions and different polymer matrices are studied, further exploring the intrinsic connection between inorganic phase migration and composite material performance. This method realizes in-situ, non-destructive, three-dimensional quantitative analysis of the early migration process of the inorganic phase, providing an effective basis for studying the structure-activity relationship of composite materials and designing high-performance composite materials. This method is simple, sensitive, accurate, and has wide applicability and practical value.
Owner:BEIJING UNIV OF CHEM TECH