22 results about "In situ visualization" patented technology

The invention relates to a method and a device for determining the flow characteristic of CO2 in a porous medium and the distribution rule of CH4 in a pore space. The method comprises the following steps: (1) pretreating a sample; (2) system connection and air tightness inspection; (3) vacuumizing the system; (4) temperature control; (5) CH4 saturation and pressure stabilization; (6) displacement experiment and data acquisition; (7) monitoring outlet gas; and (8) data processing. The problem that in existing CO2 geological sequestration and enhanced gas production research, due to the fact that in-situ visualization of the pore size cannot be achieved, a microscopic displacement mechanism is unclear is solved, and the method aims at providing scientific basis and technical support for efficient exploitation of natural gas and effective geological sequestration of CO2.

The invention relates to an all-solid-state battery thin-layer electrode device for multi-mode in-situ interface research. The all-solid-state battery thin-layer electrode device comprises the following components: a solid electrolyte substrate; the functional thin layer is arranged on the surface of the solid electrolyte substrate; the functional thin layer has conductivity; the functionalized thin layer allows a detection signal of an in-situ characterization technology to effectively penetrate or be reflected from the surface of the functionalized thin layer; the in-situ characterization techniques include atomic force microscopes, optical microscopes, and Raman spectroscopy, X-ray photoelectron spectroscopy, infrared spectroscopy, or X-ray diffraction. The invention provides a universal thin-layer electrode design and system and a thin-layer electrode in-situ imaging and spectroscopic characterization method for all-solid-state battery interface reaction. The objective of the invention is to expose a solid electrolyte-electrode interface reaction process which is difficult to observe in an effective detection range of various characterization technologies so as to realize real-time and in-situ visualization and accurate analysis of a dynamic evolution process of an internal interface of an all-solid-state battery.

The present application relates to the technical field of road engineering test, in particular to a recycled concrete interface multi-field coupling damage observation and active reinforcement device and method, and constructs a closed-loop experimental system of "observation-diagnosis-treatment" integration. The system simulates the real environment, diagnoses the state change of the internal interface area of the test piece in real time by using various high-precision sensors during the stress damage process of the test piece, and treats the weak interface at the key moment. The mechanical loading, environment simulation, in-situ microscopy, multi-parameter monitoring and active reinforcement intervention are integrated, and the whole chain and integrated research from macro performance to micro mechanism is realized. The dynamic, in-situ and visualized research on the most core scientific problem of recycled concrete is realized. Based on the trigger mechanism of multi-source information fusion, the timing of reinforcement intervention is more scientific and accurate, and data support is provided for optimizing the repair strategy.

The invention belongs to the technical field of unconventional oil and gas development, and particularly relates to a gas-water-rock reaction and particle dissolution-migration in-situ visual quantitative determination method and device based on a micro-fluidic chip. The quantitative determination method comprises the following steps: firstly, designing a chip model containing a real rock core, wherein factors such as a strong heterogeneous structure of the compact conglomerate oil reservoir and strong difference of a pore throat structure are considered at the same time; then, the gas-water-rock reaction and the particle dissolution-migration process are recorded through an optical camera; and finally, analyzing contribution degrees of different minerals to a gas-water-rock reaction and judging different corrosion modes of the conglomerate by combining image processing software and a mathematical model. The method can be used for carrying out in-situ visual quantitative determination on gas-water-rock three-phase reaction, particle corrosion and a secondary migration process in the conglomerate reservoir under the conditions of oil reservoir temperature pressure and fluid chemistry.

The invention discloses a carbon nano chemical indicator, a preparation method thereof and application of the carbon nano chemical indicator in bacterial proliferation monitoring. The method comprises the following steps: S1, preparing nitrogen-sulfur co-doped carbon quantum dots; and S2, carrying out surface modification by adopting 1, 2-naphthoquinone-4-sodium sulfonate, so as to obtain the carbon nano chemical indicator. The intelligent response type chemical indicator based on the carbon quantum dots is innovatively developed, according to the scheme, a specific response system is constructed through surface modification of the carbon quantum dots, and real-time and in-situ visual monitoring of bacterial proliferation is achieved through fluorescence / color dual-mode changes triggered by a bacterial metabolism microenvironment; compared with a traditional method, the method has the outstanding advantages that the response is fast (lt; the method can realize dynamic monitoring, does not need complex pretreatment, supports nondestructive detection, reduces the detection cost by more than 80%, has double functions of naked eye visualization and instrument quantitative detection, and is suitable for universal detection of a plurality of common bacteria.

The subject invention pertains to compositions of a probe comprising two pH-reversible forms, including the boron dipyrromethene (BODIPY) fluorescent form and the non-fluorescent leuco-BODIPY form, for use in confocal imaging methods of intracellular vesicles as well as extracellular vesicles that are present in conditioned culture medium and / or secreted by living cells, without inducing severe cytotoxicity.

The invention discloses a method for measuring the film-forming coverage rate and the shear strength of a low-speed heavy-load friction interface lubricating grease deposited film based on a fluorescence method, and belongs to the technical field of lubricating grease measurement. The method comprises the following steps: vertically arranging a coaxial illumination fluorescence microscope right above a ball-disc contact area formed by a steel ball and a sapphire disc; a lubricating grease sample dyed by a fluorescent agent is preset in the ball disc contact area; after the excitation light is filtered, the excitation light is horizontally emitted into a coaxial illumination fluorescence microscope, is reflected by a dichroscope, and is vertically irradiated to a lubricating grease sample in a ball disc contact area; a fluorescence signal sequentially passes through a dichroscope and a cut-off filter, is focused and amplified by a coaxial illumination fluorescence microscope, and is captured by a camera; and converting the fluorescence signal into a fluorescence image, and carrying out morphological and gray brightness analysis to obtain the distribution form and aggregation state of the lubricating grease thickening agent in the contact area and in the peripheral friction raceway of the contact area. According to the invention, in-situ, visual and quantitative determination of the film-forming coverage rate and the shear strength of the deposited film of the lubricating grease thickening agent is realized.

The invention discloses a method for researching an MOF (Metal Organic Framework) synthesis process by utilizing a low-field nuclear magnetic imaging device, and belongs to the technical field of material synthesis process analysis. The method comprises the following steps: firstly, preparing a metal source solution and an organic ligand solution; one of the solutions is selected for low-field nuclear magnetic resonance relaxation time measurement, and imaging sequence parameters are optimized; mixing the two solutions, and immediately putting the mixed solution into a low-field nuclear magnetic imaging device; and carrying out continuous relaxation time weighted imaging and layered relaxation time measurement on the reaction process by using the optimized imaging sequence. The lossless, in-situ, visual and quantitative monitoring of the metal-organic framework material in the nucleation, growth and sedimentation process is realized by analyzing the change of image signal intensity in time and space and the change trend of relaxation time (such as T2 value) of different liquid layers along with time. According to the method, required equipment is simple, operation is convenient and fast, and an innovative research means is provided for deeply understanding the MOF synthesis mechanism and optimizing the synthesis process.

The embodiment of the application discloses a virus three-dimensional reconstruction method and system based on three-dimensional Gaussian splash, and the method comprises the following steps: acquiring three-dimensional point cloud data of a target virus; performing molecular dynamics simulation on the target virus based on a coarse-grained molecular dynamics model, identifying a high-energy interaction region, and acquiring binding energy data of the target virus and host molecules in the interaction process; tracking the conformational change of the virus structure to obtain conformational change data; performing three-dimensional Gaussian splash processing on the point cloud data to generate an in-situ visual three-dimensional model of the target virus; and in the Gaussian splash processing, the shape, direction and weight of the Gaussian kernel are dynamically adjusted according to the binding energy data and the conformational change data. The application realizes efficient generation of a virus three-dimensional model according to the characteristics of the virus.

The present invention discloses a fluorescence in situ hybridization method for co-localization of multiple genes in shellfish hemocytes, belonging to the technical field of cell biology of marine invertebrates. By optimizing the osmotic pressure buffer system and the preparation method, the present invention can stably obtain a monolayer sample with complete and uniform cell morphology. By advancing the cell morphology imaging step, the complete morphological information of cells is obtained and fixed as a reference before implementing the stringent in situ hybridization process. The present invention can achieve in situ visualization of multiple target genes while better preserving the cell morphological structure, which helps to obtain more accurate and stable spatial distribution information of gene expression at the single-cell level, improves the overall reliability of the experiment, and provides a more effective analysis tool for the study of cell functions of marine invertebrates.

The application discloses a cell PD-L1 glycosylation in-situ visualization and biological function research method based on hybridization chain reaction. The application adopts a hybridization chain reaction strategy (HCR) of a logic "AND" gate activated by nucleic acid aptamer recognition and metabolic glycan labeling, and constructs a new method for a cell PD-L1 glycosylation in-situ visualization and biological function research method. Through HCR signal amplification induced by adjacent double recognition, the detection sensitivity of PD-L1 glycosylation on different cells is significantly improved, and subtle fluctuations in the PD-L1 glycosylation level can be accurately captured, so as to provide an analysis tool for revealing the dynamic changes under different physiological or pathological conditions. Meanwhile, the application first clarifies the immune regulation function of the PD-L1 glycosylation of dendritic cells, and further expands the research scope of tumor immune checkpoints.

The invention relates to the technical field of computer vision and natural language processing, and particularly discloses a language landscape text grammar error correction method and system based on image recognition, and the method comprises the steps: collecting an image containing a language landscape text through a mobile terminal camera; performing optical character recognition on the image, and extracting a target language text; inputting the text into the grammar error diagnosis model to obtain a diagnosis result containing an error position, an error type and a correction suggestion; and finally, on a screen of the mobile terminal, performing spatial alignment and overlapping display on a diagnosis result and a text region in the image in an augmented reality mode. According to the method, a real-time, in-situ and visual quality checking and learning tool is provided for urban managers, scenic region operators and foreign language learners directly aiming at the grammar and translation quality problems of public place language landscape texts, and the problems that traditional manual inspection efficiency is low, and an online tool cannot be linked with a physical scene for checking are effectively solved.

The invention relates to a low-freezing-point infiltration and visualization integrated detection method for improved saline soil in a seasonal frozen region. Cooperative improvement is realized around three links of low-temperature, constant-temperature and constant-flow liquid supply, in-situ visualization imaging and synchronous control and quantitative identification. The method comprises the following steps: stably supplying a tracing solution with the freezing point lower than 0 DEG C in a range of-5 DEG C to + 5 DEG C; the continuous wetting and infiltration process of the surface layer of the soil sample is kept uninterrupted; a non-destructive in-situ observation window is constructed through fixed geometry and exposure combination of a wedge-shaped or cutting edge transparent observation module, an industrial camera and annular illumination; the central control and data processing module is used for implementing timestamp alignment and closed-loop coordination on pump speed, temperature control, illumination and imaging; according to the system, interface and alignment errors are reduced, energy consumption and arrangement complexity are reduced, repeatability, comparability and efficiency of tests are improved, and a standardized data basis is provided for mechanism research, parameter inversion and model calibration under the condition of an indoor rack / transparent soil column.

The present application belongs to the technical field of unconventional oil and gas development, and particularly relates to a gas-water-rock reaction and micro-particle dissolution-migration in-situ visualized quantitative determination method and device based on a micro-fluidic chip. The quantitative determination method designs a chip model containing a real core by simultaneously considering factors such as strong heterogeneity of a dense conglomerate reservoir, strong difference of pore throat structure and the like; then records the gas-water-rock reaction and micro-particle dissolution-migration process through an optical camera; finally, combines image processing software and a mathematical model to analyze the contribution degree of different minerals to the gas-water-rock reaction and to distinguish different dissolution modes of conglomerate. The method can quantitatively determine the gas-water-rock three-phase reaction, micro-particle dissolution and secondary migration process in a conglomerate reservoir under the conditions of reservoir temperature, pressure and fluid chemistry.

The present application relates to a kind of laboratory for studying the method and device for solving near well pollution with ultrasonic visualization.The present application relates to the technical field of oil and gas field development and stimulation, and the device is composed of ultrasonic system, displacement system, visualization monitoring system and data acquisition and control system;Focused ultrasonic transducer is accurately acted on microfluidic core model in cooperation with three-dimensional displacement platform, displacement system contains annular pressure automatic tracking module to simulate formation pressure, and high-speed camera realizes pore scale dynamic observation.The method comprises the following steps: establishing pollution model, applying controllable ultrasonic wave, in-situ visualizing collecting cavitation bubble and pollutant transport process, synchronously collecting seepage data and correlating and analyzing plugging removal mechanism.The present application can real-time observe the microcosmic dynamics process of ultrasonic plugging removal, quantify the relationship between frequency, power and plugging removal efficiency, and is suitable for mechanism research and parameter optimization of various near well pollution types, and solves the "black box" problem that traditional technology cannot in-situ visualize observation.

The invention discloses a separated RUBY visual virus infection system as well as a construction method and application thereof. According to the system, a key gene of a betacyanin biosynthetic pathway is split into two parts: CYP76AD1 and a GT gene are connected in series through a 2A peptide sequence and are constructed in a plant stable expression vector, and a stably inherited transgenic plant is obtained in nicotiana benthamiana; and cloning another key gene DODA into a plant virus vector to construct a recombinant virus expression vector. According to the method, real-time, lossless and in-situ visual observation of the virus infection and movement process is achieved, the system construction method is clear, complex instruments are not needed, an efficient and convenient tool platform is provided for protein function analysis, host factor screening and infection mechanism exploration in virology research, and good expandability is achieved.

This invention discloses a benzo[selenazole] derivative, its preparation method, and its application in the preparation of RNA fluorescent probes. The benzo[selenazole] derivative provided by this invention has the following structural formula: [structural formula would be inserted here]. This benzo[selenazole] derivative is a typical fluorescently activated probe, exhibiting excellent selective recognition and binding ability for RNA molecules. It demonstrates excellent resistance to biological background interference and exhibits high binding selectivity and recognition specificity for RNA in both live and fixed cell systems. It possesses advantages such as high signal-to-noise ratio and excellent fluorescence photostability, making it suitable for real-time, dynamic fluorescence imaging detection of intracellular RNA. It can achieve in-situ visualization and tracing of changes in subcellular RNA distribution and content. The synthesis method of the benzo[selenazole] derivative provided by this invention has a clear process flow, mild and stable reaction conditions, requires no demanding equipment or special reagents, and uses commercially available and readily available conventional chemicals as raw materials. The operation is simple and easy to perform, with strong overall process operability, making it suitable for large-scale production applications.

The present application relates to the technical field of analytical detection, and particularly relates to a method for synchronously in-situ visualizing and characterizing fine structure components of lignin in plant cell walls, wherein the fine structure components of lignin include at least one of lignin synthesis precursor, lignin and hemicellulose connecting substance, G-type lignin, S-type lignin, H-type lignin and the like. The in-situ visualizing and characterizing method of the present application can realize the synchronous in-situ visualizing and characterizing of the fine structure components of lignin in plant cell walls based on the area normalization processing and characteristic peak intensity ratio of Raman image pixel points, and can eliminate the influence of the scattering volume change caused by cell structure heterogeneity and the Raman intensity difference caused by different experimental conditions on the analysis results. The present application has the advantages of label-free and high chemical specificity, and can synchronously obtain the distribution of fine structure components of lignin, and is simple and efficient, and has a wide application prospect.

This invention relates to the field of anti-corrosion coating technology and provides a self-healing coating monitoring system to reduce coating damage in coastal ship unloaders. The system includes a functionalized coating system, an embedded electrode system, a signal excitation system, an optical signal acquisition system, and a data processing and analysis system. The functionalized coating system integrates a reactive activation module that generates signals at the damage site and an internal reference calibration module that provides a stable benchmark. The system applies calibration and detection potentials and simultaneously acquires corresponding electrochemiluminescence images. The data processing and analysis system calculates a system state calibration factor based on the signal from the internal reference module and uses it to normalize and correct the healing signal image, outputting a quantified coating health status. This invention achieves in-situ, visualized, and precise quantitative assessment of self-healing events and eliminates measurement errors caused by system aging and other factors through a self-calibration mechanism, ensuring the long-term reliability of the monitoring results.

The application discloses a kind of glucose mass spectrum imaging methods based on in situ derivatization technology, belong to mass spectrum imaging detection technical field, the method is: preparation sample section and affix to conductive carrier glass;Derivatization reagent and catalyst are dissolved in volatile organic solvent, preparation derivative reagent system solution, derivative reagent system solution is sprayed on the surface of tissue section and is derived to carry out reaction;After the surface of derived section is coated with matrix, it is identified and analyzed using MALDI-MSI method;Derivatization reagent structure is as follows:In the application, the trace carbonyl-containing compound in biological tissue can be mildly and quickly derived in situ by the derivatization reagent, the mass spectrum signal response of target compound molecule is significantly improved, and the analyte delocalization (diffusion) phenomenon is obviously inhibited. The derived tissue section is analyzed using the MALDI-MSI technology, the trace carbonyl-containing compound in the biological tissue section is realized high spatial resolution in situ visual characterization, and the problems existing in the prior art are effectively solved.

The disclosure provides a mixed reality-based in-situ visualization method and system for shotcrete thickness, relating to the field of civil engineering and intelligent construction, aiming to realize in-situ visualization of shotcrete thickness detection by using mixed reality technology, the method comprising: constructing a three-dimensional grid representation of the shotcrete operation site, establishing a virtual mechanical arm corresponding to the physical mechanical arm in the shotcrete operation site by using the spatial anchor point technology, and keeping the virtual mechanical arm consistent with the physical mechanical arm in pose and spatial position; generating virtual shotcrete particles and setting particle parameters for the virtual shotcrete particles; when a virtual shotcrete particle is detected to collide with the environment grid, mapping the three-dimensional coordinates of the collision point to the grid cell in the two-dimensional height field grid, and calculating the current deposition thickness value of the grid cell; based on the deposition thickness value of the grid cell, obtaining the spraying thickness of each position point in the grid cell, and rendering the spraying thickness of each position point to the target surface through the corresponding color.

The application discloses an oil-water multiphase flow monitoring and identifying system based on microfluidics and fluorescence microscopy, and relates to the field of oil-water multiphase flow monitoring and identifying systems; comprising a microfluidic chip, a fluid injection control, a microscopic imaging, an image processing module and a produced liquid collecting device. The microfluidic chip is etched based on real core CT data and accurately simulates pore structure; the fluid injection control module realizes high-precision liquid injection and constant temperature control; the microscopic imaging module captures four-phase fluid dynamics through multi-band fluorescence imaging; the image processing module carries self-developed algorithms, can identify six types of remaining oil and complete multi-dimensional quantitative analysis, and the results can feedback injection parameters. The system solves the problems of real-time observation loss, high cost and indirect mechanism inference of traditional core experiments, realizes in-situ visualization of the displacement process, data precision and intelligent regulation and control, is suitable for chemical flooding mechanism research of complex reservoirs, and provides an efficient research platform for improving recovery efficiency.