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A core-shell nano-medicine for in situ visualized treatment of tumors and its preparation method

A nano-drug and core-shell technology, which is applied in the direction of nano-drugs, anti-tumor drugs, preparations for in vivo experiments, etc., can solve problems such as long intervals, side effects superposition, and delayed treatment timing

Active Publication Date: 2021-03-16
UNIV OF ELECTRONICS SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since the diagnosis and treatment of tumors are usually two relatively independent processes in traditional clinical applications, diagnostic reagents and treatment also need to be used separately. There are also problems such as low efficiency of diagnosis and treatment and superposition of side effects

Method used

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  • A core-shell nano-medicine for in situ visualized treatment of tumors and its preparation method
  • A core-shell nano-medicine for in situ visualized treatment of tumors and its preparation method
  • A core-shell nano-medicine for in situ visualized treatment of tumors and its preparation method

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Effect test

Embodiment 1

[0071] A core-shell nanomedicine for in situ visualization therapy of tumors, such as figure 1 As shown, it is characterized in that: a core-shell nano-medicine for in situ visualized treatment of tumors, characterized in that: the core of the core-shell nano-medicine includes: polycationic compound, and the polycationic compound passes through The immune adjuvant oligonucleotides combined by electrostatic attraction, the immune adjuvant oligonucleotides are labeled with fluorescent probes; the shell of the core-shell nano-medicine includes: a hydrophilic shell, and a hydrophilic shell An MRI imaging contrast agent chelated on the surface of the shell, the hydrophilic shell includes a negatively charged phototherapy drug modified by polydopamine, and the phototherapy drug is formed by copolymerization of aminated graphene quantum dots and a carboxyl-containing photosensitizer ; The core-shell structure is formed by electrostatic assembly between polycationic compounds and poly...

Embodiment 2

[0088] Stability experiment of nanomedicine:

[0089] Such as Figure 5 As shown, figure (a) is the different incubation time of nano-medicine of the present invention and DNase I enzyme, and figure (b) is the protective effect of different nanoparticles on CpG, wherein M is Maker, 1 is free CpG, and 2 is CpG+DNase I Enzyme, 3 is P / C+DNase I enzyme+heparin sodium, 4 is P / C+heparin sodium+DNase I enzyme, 5 is P / C@GCpD(Gd)+DNase I enzyme+heparin sodium, 6 is P / C @GCpD(Gd) + sodium heparin + DNase I enzyme.

[0090] Through agarose gel electrophoresis experiments, it was shown that the P / C@GCpD(Gd) nanoparticles of the present invention can protect CpG from being degraded by enzymes. Compared with free CpG, the P / C@GCpD(Gd) particles of the present invention can Improve the circulation time of CpG in the body to a certain extent.

[0091] Such as Image 6 As shown, figure (a) and figure (b) are respectively the change figure of particle size and PDI of P / C@GCpD (Gd) of the pr...

Embodiment 3

[0093] In vitro photothermal and photodynamic properties experiments of nanomedicine:

[0094] Such as Figure 7 As shown, under 660nm laser excitation (1W / cm 2 , 10 minutes), the nanomedicine P / C@GCpD(Gd) of the present invention has a good photothermal heating effect measured by an infrared thermal imager and a thermocouple; P / C@GCpD(Gd) was measured by a singlet oxygen probe SOSG ( Gd) has a high singlet oxygen yield and can be used in photodynamic experiments.

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Abstract

The invention relates to a core-shell nano-medicine for in situ visualized treatment of tumors, which belongs to the technical field of nano-medicine. The present invention adopts the nanoparticle formed by assembling the CpG OND labeled with the fluorescent probe and the polycationic compound as the inner core, and self-assembles the graphene quantum dot and photosensitizer copolymerization product modified by polydopamine surface on the inner core surface as the outer shell, and The MRI ion contrast agent is further chelated on the shell to form a core-shell nanomedicine. The nanomedicine of the present invention not only has excellent photodynamic / photothermal anticancer effect and good immune stimulation effect, but also can perform lesion imaging through MRI imaging contrast agent and fluorescent probe, has visualization characteristics, and realizes non-invasive real-time tracking of nano The distribution, absorption and metabolism of drugs can be further used for prognostic detection. The nano-medicine of the invention integrates combined therapy and multimodal imaging diagnosis functions, realizes the integration of tumor diagnosis and treatment, and has broad application prospects in the field of tumor diagnosis and treatment.

Description

technical field [0001] The invention belongs to the technical field of nano-medicine, and in particular relates to a core-shell nano-medicine for in-situ visualized treatment of tumors and a preparation method thereof. Background technique [0002] Due to its high degree of heterogeneity, complex microenvironment and other factors, malignant tumor is a major disease that seriously endangers public life and health, and has become a global public health problem. At present, single treatment methods, such as surgical resection, chemotherapy, radiotherapy, etc., have poor efficacy and large side effects; combining or synergistically killing tumors with multiple treatment methods is the mainstream strategy of current clinical treatment. At the same time, emerging tumor therapies, such as tumor phototherapy and immunotherapy, have emerged one after another. The development and combined use of tumor therapies provide new hope for tumor treatment. Tumor phototherapy uses laser to e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/12A61K49/18A61K9/51A61K39/39A61K41/00A61K47/55A61K47/59A61K49/00A61P35/00B82Y5/00
CPCA61K9/5146A61K39/39A61K41/0052A61K41/0057A61K49/0002A61K49/0052A61K49/0093A61K49/126A61K49/1824A61K2039/6025A61K47/55A61K47/59A61P35/00B82Y5/00A61K2300/00
Inventor 吴春惠官晓天田原刘秋月刘贻尧杨红李顺秦翔
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA
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