Fluorescent blotting membrane for in-situ visual detection of root exudates as well as preparation and application of fluorescent blotting membrane

Abstract

The invention discloses a fluorescent blotting membrane for in-situ visual detection of root exudates as well as preparation and application of the fluorescent blotting membrane. The preparation method comprises the following steps: by taking cadmium telluride quantum dots as a fluorescence detection source, including the cadmium telluride quantum dots in a molecularly imprinted polymer which takes single plant root exudates as a template, aminopropyl siloxane, phenyl siloxane and a cross-linking agent, and finally loading the molecularly imprinted polymer on a gel membrane to prepare the fluorescent imprinted membrane for in-situ visual specific detection of chlorogenic acid. The fluorescent blotting membrane can be used for in-situ visual detection of plant root exudates, can only perform specific response on specific exudates in a complex system, and is high in specific response value, safe, non-toxic and reusable.

Description

technical field [0001] The invention belongs to the field of energy and chemical industry (including chemistry, material science, energy science and technology, chemical engineering, textile science and technology, food science and technology, environmental science and technology, safety science and technology, etc.) Fluorescent blotting membrane for bit visualization detection and its preparation and application. Background technique [0002] Root exudates are the main components for the efficient activation of soil nutrients by roots, which are of great significance to improve the effectiveness of rhizosphere soil nutrients, and strongly affect the types and quantities of soil microorganisms and their distribution in the rhizosphere of plants. The relationship with soil microorganisms has always been the focus and difficulty of the scientific community. However, the decomposition of root exudates in the soil, the low content of root exudates themselves, the complex compos...

AI Technical Summary

Problems solved by technology

[0004] The existing detection methods are to collect root exudates in situ, and then separate and detect the exudates. Although the collection of plant exudates in situ can reduce the damage to plants, compared with traditional exudate collection methods, the The identification of plant exudates is more accurate and faster, but it still does not achieve the function of in-situ detection, and there are still shortcomings such as the cumbersome detection of traditional column chromatography. After collection, various root exudates are mixed together, and column chromatography is still required. Wait for the operation to perform separation and detection, the steps are cumbersome and cannot save time

At the same time, there are many parts in the in-situ collection device, which is inconvenient to assemble, and the cost of the equipment is relatively high. A certain amount of experimental space is required to install the collection and detection equipment, which cannot realize the rapid and convenient detection of root exudates.

[0005] To sum up, at present, in-situ visualization is a key and difficult point in the field of root exudates detection. Without a relatively simple and sensitive detection method, root exudates will be lost during the extraction and purification process and cause irreversible damage to the root system.

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