Fluorescent blotting membrane for in-situ visual detection of root exudates as well as preparation and application of fluorescent blotting membrane

A technology of root exudates and imprinted membranes, applied in nanotechnology for materials and surface science, analytical materials, nano optics, etc., can solve problems such as root damage, inconvenient assembly, cumbersome steps, etc., and achieve wide application and operation Convenience and high specific response value
CN113292749AActive Publication Date: 2021-08-24SOUTH CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTH CHINA AGRI UNIV
Publication Date
2021-08-24

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Abstract

The invention discloses a fluorescent blotting membrane for in-situ visual detection of root exudates as well as preparation and application of the fluorescent blotting membrane. The preparation method comprises the following steps: by taking cadmium telluride quantum dots as a fluorescence detection source, including the cadmium telluride quantum dots in a molecularly imprinted polymer which takes single plant root exudates as a template, aminopropyl siloxane, phenyl siloxane and a cross-linking agent, and finally loading the molecularly imprinted polymer on a gel membrane to prepare the fluorescent imprinted membrane for in-situ visual specific detection of chlorogenic acid. The fluorescent blotting membrane can be used for in-situ visual detection of plant root exudates, can only perform specific response on specific exudates in a complex system, and is high in specific response value, safe, non-toxic and reusable.
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Description

technical field

[0001] The invention belongs to the field of energy and chemical industry (including chemistry, material science, energy science and technology, chemical engineering, textile science and technology, food science and technology, environmental science and technology, safety science and technology, etc.) Fluorescent blotting membrane for bit visualization detection and its preparation and application. Background technique

[0002] Root exudates are the main components for the efficient activation of soil nutrients by roots, which are of great significance to improve the effectiveness of rhizosphere soil nutrients, and strongly affect the types and quantities of soil microorganisms and their distribution in the rhizosphere of plants. The relationship with soil microorganisms has always been the focus and difficulty of the scientific community. However, the decomposition of root exudates in the soil, the low content of root exudates themselves, the complex compos...

Claims

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