Fluorescent blotting membrane for in-situ visual detection of root exudates as well as preparation and application of fluorescent blotting membrane

A technology of root exudates and imprinted membranes, applied in nanotechnology for materials and surface science, analytical materials, nano optics, etc., can solve problems such as root damage, inconvenient assembly, cumbersome steps, etc., and achieve wide application and operation Convenience and high specific response value

Active Publication Date: 2021-08-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing detection methods are to collect root exudates in situ, and then separate and detect the exudates. Although the collection of plant exudates in situ can reduce the damage to plants, compared with traditional exudate collection methods, the The identification of plant exudates is more accurate and faster, but it still does not achieve the function of in-situ detection, and there are still shortcomings such as the cumbersome detection of traditional column chromatography. After collection, various root exudates are mixed together, and column chromatography is still required. Wait for the operation to perform separation and detection, the steps

Method used

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  • Fluorescent blotting membrane for in-situ visual detection of root exudates as well as preparation and application of fluorescent blotting membrane
  • Fluorescent blotting membrane for in-situ visual detection of root exudates as well as preparation and application of fluorescent blotting membrane
  • Fluorescent blotting membrane for in-situ visual detection of root exudates as well as preparation and application of fluorescent blotting membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] (1) Preparation of CdTe quantum dots

[0063] Mix 1.6g sodium borohydride and 1.6g tellurium powder evenly, then disperse in 100mL distilled water, and react in the dark for 6h at room temperature to obtain the Te precursor NaHTe solution in CdTe quantum dots. Dissolve 0.4g of cadmium chloride and 0.5mL of thioglycolic acid (TGA) in 50mL of distilled water, add a 5% NaOH solution to adjust the pH to 10, and add 25mL of the precursor NaHTe solution to the In the mixed solution, the mixed solution was heated in a water bath to 90° C. for reflux reaction for 3 h to obtain a CdTe QDs solution, which was washed three times with absolute ethanol, centrifuged and freeze-dried for 12 h to obtain a CdTe QDs powder.

[0064] (2)CdTe@SiO 2 Preparation of @MIPs Molecularly Imprinted Polymers

[0065] Preparation of CdTe@SiO by Inverse Microemulsion Method 2 @MIPs: Add 100mL cyclohexane, 10mL NP-10, and 1mL distilled water into a round bottom flask, and sonicate for 20min to form...

Embodiment 2

[0069] (1) Preparation of CdTe quantum dots

[0070] 1.6g sodium borohydride (NaBH 4 ) and 3.2g of tellurium powder were mixed evenly, then dispersed in 100mL of distilled water, and reacted in the dark for 6h at room temperature to obtain the Te precursor NaHTe solution in CdTe quantum dots. Dissolve 0.5g of cadmium chloride and 0.5mL of thioglycolic acid (TGA) in 50mL of distilled water, add a 5% NaOH solution to adjust the pH to 10, and then add 17mL of the precursor NaHTe solution to the In the mixed solution, the mixed solution was heated in a water bath to 120° C. for reflux reaction for 3 h to obtain a CdTe QDs solution, washed three times with absolute ethanol, centrifuged and freeze-dried for 12 h to obtain a CdTe QDs powder.

[0071] (2)CdTe@SiO 2 Preparation of @MIPs Molecularly Imprinted Polymers

[0072] Preparation of CdTe@SiO by Inverse Microemulsion Method 2 @MIPs: Add 100mL cyclohexane, 14mL NP-10, and 1mL distilled water into a round bottom flask, and son...

Embodiment 3

[0076] (1) Preparation of CdTe quantum dots

[0077] 3 g sodium borohydride (NaBH 4 ) and 3g of tellurium powder were evenly mixed, then dispersed in 100mL of distilled water, and reacted in the dark for 12h at room temperature to obtain the Te precursor NaHTe solution in CdTe quantum dots. 0.5g cadmium chloride (CdCl 2) and 1.5 mL of thioglycolic acid (TGA) were dissolved in 50 mL of distilled water, and a 5% NaOH solution was added to adjust the pH to 10. After 0.5 h of nitrogen deaeration, 75 mL of the precursor NaHTe solution was added to the mixed solution, The mixed solution was heated to 90°C in a water bath for reflux reaction for 9h to obtain a CdTe QDs solution, washed with absolute ethanol for 3 times, centrifuged and freeze-dried for 12h to obtain CdTe quantum dot powder.

[0078] (2) CdTe@SiO 2 Preparation of @MIPs Molecularly Imprinted Polymers

[0079] Preparation of CdTe@SiO by Inverse Microemulsion Method 2 @MIPs: Add 100 mL of cyclohexane, 10 mL of NP-10...

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PUM

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Abstract

The invention discloses a fluorescent blotting membrane for in-situ visual detection of root exudates as well as preparation and application of the fluorescent blotting membrane. The preparation method comprises the following steps: by taking cadmium telluride quantum dots as a fluorescence detection source, including the cadmium telluride quantum dots in a molecularly imprinted polymer which takes single plant root exudates as a template, aminopropyl siloxane, phenyl siloxane and a cross-linking agent, and finally loading the molecularly imprinted polymer on a gel membrane to prepare the fluorescent imprinted membrane for in-situ visual specific detection of chlorogenic acid. The fluorescent blotting membrane can be used for in-situ visual detection of plant root exudates, can only perform specific response on specific exudates in a complex system, and is high in specific response value, safe, non-toxic and reusable.

Description

technical field [0001] The invention belongs to the field of energy and chemical industry (including chemistry, material science, energy science and technology, chemical engineering, textile science and technology, food science and technology, environmental science and technology, safety science and technology, etc.) Fluorescent blotting membrane for bit visualization detection and its preparation and application. Background technique [0002] Root exudates are the main components for the efficient activation of soil nutrients by roots, which are of great significance to improve the effectiveness of rhizosphere soil nutrients, and strongly affect the types and quantities of soil microorganisms and their distribution in the rhizosphere of plants. The relationship with soil microorganisms has always been the focus and difficulty of the scientific community. However, the decomposition of root exudates in the soil, the low content of root exudates themselves, the complex compos...

Claims

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Application Information

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IPC IPC(8): C08J5/18
CPCC08J5/18C08J9/28C08G77/26C08K3/30C08K3/36C09K11/025C09K11/883B82Y20/00B82Y30/00B82Y40/00G01N21/643G01N2021/6432C08J2201/0482C08J2383/08C08J2301/28C08J2405/12C08J2489/00C08J2483/08C08J2329/04C08J2401/28C08J2405/16
Inventor 蒋刚彪蓝玲敏胡甜李伟雄吴官平
Owner SOUTH CHINA AGRI UNIV
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