A fluorescent blotting membrane for in situ visual detection of root exudates and its preparation and application

Abstract

The invention discloses a fluorescent imprinting membrane for in-situ visual detection of root exudates and its preparation and application. The invention uses cadmium telluride quantum dots as a fluorescence detection source, and incorporates them into a molecularly imprinted polymer with a single plant root exudate as a template, aminopropylsiloxane, phenylsiloxane and a cross-linking agent, Finally, it was loaded on a gel membrane to prepare a fluorescent blotting membrane for in situ visualization and specific detection of chlorogenic acid. The obtained fluorescent blotting membrane can visually detect exudates from plant root exudates in situ, can only respond specifically to specific exudates in a complex system, has a high specific response value, is safe, non-toxic, and can be reused.

Description

technical field [0001] The invention belongs to the field of energy and chemical industry (including chemistry, material science, energy science and technology, chemical engineering, textile science and technology, food science and technology, environmental science and technology, safety science and technology, etc.) Fluorescent blotting membrane for bit visualization detection and its preparation and application. Background technique [0002] Root exudates are the main components of efficiently activating soil nutrients in the root system, which are of great significance to improving the availability of soil nutrients in the rhizosphere, and strongly affect the type and quantity of soil microorganisms and their distribution in the rhizosphere of plants. The relationship with soil microorganisms has always been the focus and difficulty of the scientific community. However, the decomposition of root exudates in the soil, the low content of root exudates themselves, the compl...

AI Technical Summary

Problems solved by technology

[0004] The existing detection methods are to collect root exudates in situ, and then separate and detect the exudates. Although the collection of plant exudates in situ can reduce the damage to plants, compared with traditional exudate collection methods, the The identification of plant exudates is more accurate and faster, but it still does not achieve the function of in-situ detection, and there are still shortcomings such as the cumbersome detection of traditional column chromatography. After collection, various root exudates are mixed together, and column chromatography is still required. Wait for the operation to perform separation and detection, the steps are cumbersome and cannot save time

At the same time, there are many parts in the in-situ collection device, which is inconvenient to assemble, and the cost of the equipment is relatively high. A certain amount of experimental space is required to install the collection and detection equipment, which cannot realize the rapid and convenient detection of root exudates.

[0005] To sum up, at present, in-situ visualization is a key and difficult point in the field of root exudates detection. Without a relatively simple and sensitive detection method, root exudates will be lost during the extraction and purification process and cause irreversible damage to the root system.

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