A kind of immunoprobe based on polymer luminescence point and its preparation method and application

A polymer and luminous point technology, applied in the field of detection, can solve the problems of heavy metal pollution and other problems, and achieve the effect of reliable method, elimination of interference and strong specificity

Active Publication Date: 2018-07-13
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, traditional quantum dots are mainly cadmium-based quantum dots, which are polluted by heavy metals. Therefore, it is necessary to prepare and apply probes made of environmentally friendly materials.

Method used

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  • A kind of immunoprobe based on polymer luminescence point and its preparation method and application
  • A kind of immunoprobe based on polymer luminescence point and its preparation method and application
  • A kind of immunoprobe based on polymer luminescence point and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1 Preparation of an immunoprobe based on polymer luminescent dots

[0072] (1) Preparation of imidophos polyclonal antibody

[0073] According to the literature (Xu Ying. Research on immunoassay methods of organophosphorus pesticides dichlorvos and imophos [D]. Huazhong Agricultural University, 2009.) Mannich method was used to couple Phosemet to cationic bovine serum albumin (cBSA) to construct Phosemet complete antigen. According to the literature (Song Yang. Research on the ELISA method of imidophos pesticide residues [D]. Tianjin University of Science and Technology, 2009), the rabbit was immunized with the imothion antigen immunization scheme, and the antiserum titer reached the highest after 66 days. Blood was collected from the heart, rabbit serum was extracted, centrifuged to obtain imidophos antibody, and the concentration range of the antibody obtained by ultraviolet spectrum measurement was 0.64-0.78mg / mL, and stored at -20°C for later use.

[0074]...

Embodiment 2

[0097] Example 2 Detection of Stability and Specificity of Immunoprobes Based on Polymer Luminescent Dots

[0098] (1) According to the optimal reaction conditions of Example 1, the immune probe based on the polymer luminescent point was prepared. The optimal reaction conditions were as follows: the reaction mass ratio of PDs to antibodies was 4:5, the reaction temperature was 37°C, and the reaction time was 2h. The pH value of the system is 7.3. The prepared immune probe (PDs-Ab solution) was tested in the following steps.

[0099] (2) Detection of stability: store the immunoprobe based on the polymer luminescent point at 4°C. During the first week of storage, there was little change in fluorescence intensity. After that, it was measured once every other week, and the fluorescence intensity decreased slightly with the increase of time, and after 30 days of storage, the fluorescence intensity decreased by 10%. The above results show that the fluorescent properties of the pr...

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Abstract

The invention discloses an immunity probe based on a polymer luminous point and a preparing method and application thereof.The preparing method includes the steps that CN-PPV and PSMA are reacted, and a PDs aqueous solution is prepared and obtained; the PDs aqueous solution, PBS and EDC are mixed to be even to obtain mixed liquid A; the mixed liquid A and phosmet antibodies are mixed and reacted, and the immunity probe based on the polymer luminous point is obtained.The immunity probe can specifically recognize phosmet, and therefore the immunity probe can be used for quantitatively detecting phosmet and self-quantitatively or quantitatively detecting phosmet.The qualitative and self-quantitative detecting method is an in-situ visual detecting method; the quantitative detecting method is high in sensitivity and specificity, and high-sensitivity quantitative detection can be carried out.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to an immunoprobe based on a polymer luminescence point and its preparation method and application. Background technique [0002] The impact of pesticides on food, environment and ecology has attracted more and more attention. Pesticide residue samples are characterized by complex components and low analyte content, and the determination of its content is an important and challenging task. Since the content of pesticide residues is closely related to people's daily life, establishing a highly sensitive and on-site visual pesticide detection method is one of the future development directions. [0003] Imophos is an organophosphorus pesticide commonly used at present. The most commonly used detection methods mainly include high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS), but it usually requires complicated sample pretreatment. The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/533
CPCG01N33/533
Inventor 俞英周丽娜曹玉娟林碧霞水玲玲
Owner SOUTH CHINA NORMAL UNIVERSITY
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