15 results about "Nitrosylation" patented technology

Disclosed is a process for the synthesis of mono- and bis-nitrosylated propanediols, as well as compositions and pharmaceutical formulations that includes the compounds. The process proceeds by reacting a corresponding propanediol that is not nitrosylated with a source of nitrite, optionally in the presence of a suitable acid. When the source of nitrite is an organic nitrite, reacting step is performed in a suitable organic solvent, and when the source of nitrite is an inorganic nitrite, the reacting step is performed in a bi-phasic solvent mixture comprising an aqueous phase and a non-aqueous phase. Also disclosed are methods of treating a condition wherein administration of nitric oxide (NO) has a beneficial effect by administering said compounds, compositions or formulations.

The invention relates to the field of bioengineering, in particular to application of GluA2 protein in an mPFC region as a target spot in preparation of a medicine for treating post-stroke depression. According to the application disclosed by the invention, by establishing a PSD model and detecting depression-like behaviors, the action and mechanism of the surface expression of an NSF protein S-nitrosation modification mediated GluA2-conforming AMPA receptor in the PSD are researched, the S-nitrosation level of NSF in an mPFC region and the interaction of GluA2-NSF are adjusted to be a target spot with clinical application potential for treating post-stroke depression, and the application has the advantages that the application range is wide, and the application prospect is wide. The method is beneficial for designing a medicine and a treatment method for treating post-stroke depression aiming at the NSF-GluA21, and has important scientific value and application prospect.

A method of treating cardiovascular disorders and diseases in a subject in need thereof includes administering to the subject an ADH inhibitor, AKR inhibitor, and / or SNO-CoAR inhibitor at an amount(s) effective to promote S-nitrosylation of proteins in the subject, wherein the ADH inhibitor and / or SNO-CoAR inhibitor is not an ADH3 inhibitor.

The invention relates to the technical field of microbial infection treatment and molecular biology, and provides a method for inhibiting formation of a staphylococcus aureus biofilm, which is realized by inhibiting nitrosation modification of MgrA protein in staphylococcus aureus so as to inhibit formation of the biofilm. The inhibitor is nitric oxide synthase and is used for inhibiting nitrosation modification of the MgrA protein; the nitrosation modification for inhibiting the MgrA protein is realized by applying a nitric oxide synthase (NOS) inhibitor; the invention reveals that the staphylococcus aureus endogenous nitric oxide can promote the formation of a biofilm by modifying and regulating the 12th cysteine of the protein MgrA for the first time; based on the target spot, research proves that the MgrA modification level can be effectively reduced and the removal of the biofilm can be enhanced through multiple means such as gene mutation, NOS inhibitor or combination of the NOS inhibitor and antibiotics, and a new strategy and a potential drug development approach are provided for preventing and treating staphylococcus aureus intractable biofilm infection.

The invention discloses a method for extracting bamboo leaf flavonoid and preparing a bamboo leaf flavonoid health-care beverage and beverage, and particularly relates to a method for preparing the bamboo leaf flavonoid health-care beverage and beverage by using nine high-benefit bamboo varieties in southern China as raw materials. The high-temperature-resistant coating is prepared from the following raw materials in parts by weight. The components comprise 70 parts of flavonoid compounds and other effective active substances extracted from dry bamboo leaf fragments by adopting a complex enzyme-microwave method, 16 parts of polysaccharide extracted from hericium erinaceus by adopting a conventional hot water extraction method, 17 parts of effective active substances extracted from plumbago indica by adopting hot water, 9 parts of eurycoma longifolia, 26 parts of effective active substances of beautiful millettia roots and 15 parts of effective active substances of polygonatum kingianum. The bamboo leaf flavonoid health-care beverage and the bamboo leaf beverage integrate the nutrients and other effective components of the bamboo leaf flavonoid and the hericium erinaceus and plumbago indica extract liquor, become an excellent bamboo leaf flavonoid health-care beverage and the bamboo leaf beverage, and have the effects of eliminating various types of reactive oxygen free radicals and inhibiting lipid peroxidation after being drunk usually; the vitamin C can protect brain blood vessels, block nitrosation reaction and eliminate nitrous ammonia, and can resist fatigue and aging as vitamin C.

Disclosed herein are materials, articles, and methods for reducing, mitigating, or excluding the formation and / or amount of nitrosylating agents and / or N-nitroso compounds, including N-nitrosamines, in a package containing an active agent and a pharmaceutical dosage form in a shell, wherein the active agent can be effective to reduce, mitigate, or exclude the formation and / or amount of nitrosylating agents and / or N-nitroso compounds in the shell and / or in the pharmaceutical dosage form. There is provided a drug delivery system comprising a blister pack configured to contain a plurality of drug dosage forms, the blister pack comprising an active ingredient, the active ingredient may be effective to reduce, mitigate, or exclude the formation and / or amount of nitrosylating agents and / or N-nitroso compounds in the outer shell of the blister pack and / or in the pharmaceutical dosage form.

The invention discloses a protein sulfur-nitrosation modified detection probe as shown in a formula I. A preparation method comprises the following steps: (1) carrying out condensation reaction on 2-(diphenylphosphino) benzoic acid and a sulfydryl alkyl alcohol compound II to obtain an intermediate III; (2) carrying out acyl chloride condensation reaction on the intermediate III and phosphorus oxychloride under an alkaline condition, and then hydrolyzing to obtain a monophosphate intermediate IV; and (3) carrying out condensation reaction on the intermediates III and IV and a monophosphate compound to obtain the compound as shown in the formula I. The probe provided by the invention can selectively react with sulfur-nitrosation modified protein, so that the probe can be applied to immunoblotting, fluorescence microscopic imaging, enzyme-linked immunosorbent assay and proteomics analysis to detect SNO modification of the protein.

The invention discloses a protein nitrosation modification regulation compound as shown in a formula I as well as a preparation method and application of the protein nitrosation modification regulation compound. The compound disclosed by the invention can be used for simultaneously combining calcium / calmodulin dependent kinase II alpha (CaMKII alpha) and nerve type nitric oxide synthase (nNOS), promoting the interaction of the two proteins and up-regulating the nitrosation modification level of the CaMKII alpha, and can be used for preventing and / or treating diseases mediated by the CaMKII alpha.

The invention discloses an integral centrifugal column for enriching S-nitrosopeptide as well as a preparation method and application of the integral centrifugal column, and belongs to the technical field of SNO proteomics analysis. The method disclosed by the invention comprises the following steps: adding an integral material pre-polymerization solution into a closed blank centrifugal column, and carrying out in-situ thermal initiation polymerization reaction to obtain a substrate integral material centrifugal column; and swelling the substrate integral material centrifugal column, adding a phosphate buffer solution of pyridine dithio ethylamine hydrochloride, standing and cleaning to obtain the integral material centrifugal column for enriching the SNO peptide. The centrifugal column can realize integrated reduction, marking and enrichment of SNO peptides, and is applied to SNO proteomics analysis in complex biological samples. The method solves the technical problem that the existing method is difficult to simultaneously reduce / mark / enrich SNO protein or peptide in a complex sample, effectively reduces the loss and degradation of the SNO peptide in the sample treatment process through the integrated sample treatment process, and obviously improves the identification scale of the SNO protein and the modification site.

The application discloses a kind of nitric oxide donor type raw ginger exosome fusion liposome nanodelivery system and its preparation method and application, the nanodelivery system uses super flow control chip technology to nitric oxide precursor, two stearyl phosphatidylcholine, cholesterol, tobramycin, self-assembly, and then prepared by extrusion method and raw ginger exosome membrane fusion.The drug delivery system has a significant breakthrough in mucus barrier and antibacterial efficacy, and can be applied to inhalation therapy for pseudomonas aeruginosa lung infection.The negative surface of the drug delivery system and the electrostatic repulsion of mucin in mucus, mPEG-PNTC generates NO due to GSH responsiveness, which has a gas motor effect, enhancing the effect of breaking through the airway mucus barrier, while the NO generated by mPEG-PNTC, through nitrosylation and oxidative stress, destroys bacterial biofilm, interferes with bacterial iron metabolism, and synergistically kills bacteria and destroys biofilm.The present application has the advantages of improving drug delivery efficiency and high-efficiency bactericidal function, and has broad clinical application prospects and development value in the field of bacterial lung infection treatment.

To provide an S-nitrosylation inhibitor of IRE1α and screening method thereof.SOLUTION: Provided is an S-nitrosylation inhibitor of IRE1α represented by the following formula (I): (A is a monovalent α,β-unsaturated carbonyl group; B and C are the same or different and either (i) or (ii): (i) B and C together form a substituted / unsubstituted monocyclic or polycyclic ring, the monocyclic or polycyclic ring containing at least a 6- or 7-membered heterocyclic ring; and (ii) B and C are the same or different and are a substituted / unsubstituted monovalent lower alkyl group, a substituted / unsubstituted monovalent aryl group, or a substituted / unsubstituted monovalent cyclic group which may contain a heteroatom, provided that B and C are not both lower alkyl groups; and L1 and L2 are the same or different and are divalent linkers having 1 to 4 carbon atoms, provided that when B and C together form a monocyclic or polycyclic ring, L1 and L2 are absent).SELECTED DRAWING: None

Aspects of the present disclosure are directed to methods, compositions, and kits for detection and analysis of DNA and RNA modifications, such as DNA and RNA nucleobase methylation. Certain aspects include methods, compositions and kits useful in sequencing of methylated nucleic acids, including methylated nucleic acids from low-input samples such as cell-free DNA and cell-free RNA. Certain aspects include novel N-nitrosation strategies for deamination of DNA and RNA biological macromolecules under mild conditions. In some aspects, provided are compositions, methods, and kits comprising means for cooperative catalysis of a carbonyl organocatalyst with a Lewis acid to facilitate the formation of a C-nitro intermediate from a primary amine, which, upon rearrangement into N-nitrosamine, leads to selective deamination of unsubstituted canonical DNA and RNA bases under mild conditions.

The application discloses a covalent kinase inhibitor, a preparation method, a pharmaceutical composition and application, the structure of the covalent kinase inhibitor is as shown in formula I, II, III, IV or V, and the covalent kinase inhibitor further comprises a pharmaceutically acceptable salt thereof, the covalent kinase inhibitor can be efficiently covalently combined with a target kinase, and simultaneously triggers the release of NO / NO2 — ; on one hand, the covalent combination can covalently inhibit the target kinase containing a sulfydryl group, and on the other hand, the released NO / NO2 — can further produce sulfydryl nitroso modification on the target kinase or a protein interacting with the target kinase, thereby inhibiting self-phosphorylation, preventing kinase signal transduction and playing a synergistic effect; the covalent kinase inhibitor can act on various kinases related to malignant tumor proliferation, has good selectivity and a low off-target effect, can significantly inhibit the growth of tumor cells at a cell and animal level, and has no obvious adverse reactions. In addition, the preparation method is simple and universal, and is conducive to the expansion of a mother nucleus structure of various other kinase inhibitors.

The present invention relates to a novel method of synthesizing mononitrosylated and dinitrosylated propanediols and novel compositions and pharmaceutical formulations comprising said compounds. The method is carried out by reacting the corresponding non-nitrosylated propanediol with a source of nitrite, optionally in the presence of a suitable acid. Therein, when the source of nitrite is an organic nitrite, the reaction step is performed in a suitable organic solvent and when the source of nitrite is an inorganic nitrite, the reaction step is performed in a biphasic solvent mixture comprising an aqueous phase and a non-aqueous phase. The present invention further relates to methods of treating conditions in which the administration of nitric oxide (NO) has a beneficial effect, by administering said compounds, compositions or formulations.

This invention discloses a method for simultaneously detecting protein phosphorylation and S-nitrosylation. It utilizes phosphate affinity tags to label S-nitrosylated proteins, transforming the S-nitrosylation modification into a phosphorylation-mimicking tag. This allows for the simultaneous enrichment and analysis of both S-nitrosylation and phosphorylation using conventional phosphorylated proteomics techniques. Through testing on model proteins and complex biological samples, we have verified that this method exhibits high sensitivity, good reproducibility, and broad protein coverage, comparable to traditional single-method enrichment. It overcomes the technical challenges of analyzing modifications with different chemical properties and provides a reliable tool for comprehensive research on cross-functional PTM (phosphorylated protein-to-protein) interactions.