48 results about "Confocal laser scanning microscope" patented technology

A laser scanning microscope produces molecular excitation in a target material by simultaneous absorption of three or more photons to thereby provide intrinsic three-dimensional resolution. Fluorophores having single photon absorption in the short (ultraviolet or visible) wavelength range are excited by a beam of strongly focused subpicosecond pulses of laser light of relatively long (red or infrared) wavelength range. The fluorophores absorb at about one third, one fourth or even smaller fraction of the laser wavelength to produce fluorescent images of living cells and other microscopic objects. The fluorescent emission from the fluorophores increases cubicly, quarticly or even higher power law with the excitation intensity so that by focusing the laser light, fluorescence as well as photobleaching are confined to the vicinity of the focal plane. This feature provides depth of field resolution comparable to that produced by confocal laser scanning microscopes, and in addition reduces photobleaching and phototoxicity. Scanning of the laser beam by a laser scanning microscope, allows construction of images by collecting multi-photon excited fluorescence from each point in the scanned object while still satisfying the requirement for very high excitation intensity obtained by focusing the laser beam and by pulse time compressing the beam. The focused pulses also provide three-dimensional spatially resolved photochemistry which is particularly useful in photolytic release of caged effector molecules, marking a recording medium or in laser ablation or microsurgery. This invention refers explicitly to extensions of two-photon excitation where more than two photons are absorbed per excitation in this nonlinear microscopy.

For the purpose of calibration which is simple and can also be carried out as often as desired, an arrangement and a method for calibrating a preferably confocal laser scanning microscope, it being possible for an object (1) to be scanned by a scanning beam (2), are defined by calibration means (12) which are arranged in the plane of an intermediate image (11) and can likewise be scanned by the scanning beam (2).

A method of finding, recording and optionally evaluating object structures, especially on slides, preferably of fluorescent object structures such as gene spots. A microscope with a CCD camera, a scanning microscope or a preferably confocal laser scanning microscope can be used for recording and for the rapid and reliable detection of the object structures, with the image data being recorded using an illumination pattern that is projected into the object plane.

In a confocal laser scanning microscope with an illuminating configuration (2), which provides an illuminating beam for illuminating a specimen region (23), with a scanning configuration (3, 4), which guides the illuminating beam over the specimen while scanning, and with a detector configuration (5), which via the scanning configuration (3, 4) images the illuminated specimen region (23) by means of a confocal aperture (26) on to at least one detector unit (28), it is provided that the illuminating configuration (2) of the scanning configuration (3, 4) provides a line-shaped illuminating beam, that the scanning configuration (3, 4) guides the line-shaped illuminating beam over the specimen f while scanning and that the confocal aperture is designed as a slit aperture (26) or as a slit-shaped region (28, 48) of the detector unit (28) acting as a confocal aperture.

The invention provides a method for determining a diagenetic process and a porosity evolution process of a foreland basin sandstone reservoir. The method comprises steps as follows: determining reservoir genesis and petrologic features; determining a burial process and tectonic uplift and subsidence periods; determining a diagenesis type, features and strength of the reservoir by a cathodoluminescence microscope and / or through QEMScan (Quantitative Evaluation of Minerals by SCANning electron microscopy), and recovering the diagenetic process; determining reservoir space features and the porosity evolution process by a fluorescence microscope and / or a confocal laser scanning microscope. With the adoption of the method, the diagenetic process and the porosity evolution process of the ultra-deep compact foreland basin sandstone reservoir with complicated burial process, extremely great depth, extremely small pores and extremely low porosity and permeability can be effectively analyzed; the method can be used for predicating the quality of the sandstone reservoirs in different areas in the plane and the vertical profile, so that more and larger oil and gas fields can be discovered.

A laser microscope includes: a light source that emits deep ultraviolet laser light to be irradiated on a sample; a limiting device that sets a limit to an intensity of the deep ultraviolet laser light irradiated on the sample; a detection device that detects the deep ultraviolet laser light having been reflected from the sample; a storage device that stores first information related to damage to the sample corresponding to the intensity of the deep ultraviolet laser light; and a control device that controls the limiting device based upon the first information when a sample damage limit is input from outside.

The invention relates to a method of measuring the surface roughness by using a confocal laser scanning microscope, which belongs to the technical field of the physical performance examination on metal materials. According to the method, through the technical steps of measurement on a standard sample block, measurement on a to-be-measured sample and the like, the problem that the 3D (Three-Dimensional) shape of a to-be-measured surface can not be obtained in the traditional method. The method has the advantages that the a non-contact mode is realized, the surface can not be damaged, the sample can be put without requirements on directivity, the 3D image microstructure of the surface can be displayed clearly and directly, and especially, calibrating procedures on the standard sample block are conducted in a simple and reliable mode so as to keep measurement results of samples to be consistent and traceable.

An image recording method for use in a confocal laser scanning microscope apparatus is configured to scan a specimen with each of a plurality of laser lights at least having different wavelengths as spotlight, to detect the light from the specimen based on the spotlight, and to partition and record obtained image information.

The invention relates to an electric real-time adjustment method and an electric real-time adjustment system of a two-dimensional pinhole for a confocal laser scanning microscope. The electric real-time adjustment system comprises an X linear stepping motor, an X driving nut, a Y linear stepping motor, a Y driving nut, an X guide rail, an X sliding block, a Y guide rail, a Y sliding block, an X optocoupler, an X separation blade, a Y optocoupler, a Y separation blade, a light splitting plate, an imaging lens, a CCD (Charge Coupled Device), an electric control unit and the pinhole, wherein the X driving nut, the X sliding block and the X separation blade are mounted in a fastening manner, and the X sliding block is in sliding connection with the X guide rail; the Y driving nut, the Y sliding block and the Y separation blade are mounted in the fastening manner, and the Y sliding block is in sliding connection with the Y guide rail; the X driving nut does a linear motion under the driving of the X linear stepping motor; the Y driving nut does a linear motion under the driving of the Y linear stepping motor; the Y guide rail is fastened on the X sliding block; the pinhole is fastened in the Y sliding block. The electric real-time adjustment method and the electric real-time adjustment system can realize real-time electric adjustment on the two-dimensional direction position of the pinhole, and the influence caused by light spot position changes of laser introduced by other optical components in the system is eliminated.

The invention relates to the technical field of coal injection, and especially relates to an experimental method for determining the fusion characteristics of coal ash for spraying. The method comprises the steps that: coal ash is calcined into a coal ash mass; the coal ash mass is placed under a high-temperature confocal laser scanning microscope, and a coal ash mass morphological change process is observed; and initial coal ash deformation temperature, initial liquid phase formation temperature, and liquid phase complete fusion temperature of the coal ash mass are determined. According to the experimental method for determining fusion characteristics of coal ash for spraying, heating speed of the high-temperature confocal laser scanning microscope is high, and spraying coal ash fusion characteristic experiment efficiency is high. The method provided by the invention has high popularization value.

The invention discloses a coaxiality adjusting method of multiple beams. The method comprises the following steps that: step (1), a plurality of reflectors or beam splitters are arranged at light paths of a plurality of laser light sources, so that light emitted by the multiple laser light source is combined into one beam of light that can not be distinguished by naked eyes; step (2), a particulate matter that can reflect laser is selected, wherein the particle size of each particle of the particulate matter is less than the diameter of the light spot of the light beam that can not be distinguished by naked eyes; step (3), with an imaging device arranged at a confocal laser scanning microscope, scanning imaging is carried out on the particulate matter by the light beam that can not be distinguished by naked eyes; and step (4), if the obtained image contains a double image, the light beam that can not be distinguished by naked eyes is not a coaxial one, and the reflectors or beam splitters are adjusted and the step (3) is carried out again; and if no double image exists in the obtained image, the light beam that can not be distinguished by naked eyes is the coaxial one and thus adjustment is completed.

The invention relates to a confocal laser scanning microscope. An objective table of the confocal laser scanning microscope is additionally provided with a heating device; a temperature measuring device and an infrared detector are additionally arranged between an objective lens and a sample platform of the confocal laser scanning microscope; and heat image processing software is additionally arranged in a computer system of the confocal laser scanning microscope. The confocal laser scanning microscope relates to the following novel detection characterization techniques: (1) the microstructures of materials can be researched; (2) the distribution condition in a large range is directly observed by applying impurity or doped phase fluorescence excitation; (3) fluorescence is captured to dynamically track; (4) a system is focused for many times to obtain images with different depths; and three-dimensional imaging can be implemented for samples through computer processing; and (5) the heat and mass transfer processes of various materials can be synchronously dynamically researched when the temperature is changed, so that material phase changed thermodynamics and dynamics mechanisms can be built.

The invention discloses an intracellular micro-RNA non-enzymatic amplification detection method based on an electrostatic affinity nano-transporter and cell imaging. According to the method, sequencesof specific DNA (deoxyribonucleic acid) hairpin probes H1 and H2 with fluorescent labels are designed by the aid of a non-enzymatic amplification system according to a detection target micro-RNA sequence, the H1, the H2 and gold nano-particles are assembled into compounds to be transferred to tumor cells, the amplification system and the H1 can be combined when the amplification system contains micro-RNA to be detected, so that the neck of the H1 is opened, parts of generated single-chain structures and the H2 act, the neck of the H2 is opened, the distance between a fluorescent group FAM andBHQ-1 quenching group is increased, fluorescence strength is increased and detected by a fluorescence spectrometer, and intracellular imaging conditions are recorded by a confocal laser scanning microscope. The method is simple in principle, low in detection cost and has the advantages that constant-temperature non-enzymatic amplification is achieved, and the method is high in sensitivity, simpleto operate, easy to popularize and the like.

The invention belongs to the field of medical devices, and in particular relates to a diagnosis and treatment integration confocal colposcopic system. The diagnosis and treatment integration confocal colposcopic system comprises a hard colposcope, wherein a treatment device is arranged on the hard endoscopic end part of the hard colposcope, and a treatment system host which is correspondingly matched with the treatment device is connected to the hard colposcope. Specifically, the diagnosis and treatment integration confocal colposcopic system organically combines the hard colposcope and a confocal laser scanning microscope system with a laser scalpel system or a microwave scalpel system. Through the clinical application of the diagnosis and treatment integration confocal colposcopic system, an effect of diagnosing and treating at the same time can be realized, one colposcopic can realize two problems of diagnosis and treatment simultaneously, endoscopes are prevented from being frequently changed, and the corresponding treatment can be immediately carried out, so that surgical time is greatly saved, and the pain of a patient is relieved; and furthermore, the surgical accuracy and safety are improved, and an unexpected effect is realized.

The invention belongs to the technical field of molecular biology and gene engineering, and discloses a method for preparation and transient gene expression of a jatropha curcas tissue culture seedling protoplast. The method comprises the following operation steps that jatropha curcas tissue culture seedling leaves are cut into leaf strips with the width of 0.5-1 mm, the leaf strips are placed into an enzymatic hydrolysate for light shading, standing is performed at the temperature of 22-25 DEG C for enzymolysis for 6-7 h, filtration washing is performed on the protoplast, a certain amount of plasmid DNA, the proroplast and the PEG4000 / Ca2+ solution are added for processing, culture is performed for 18-40 h and then observation is performed under a confocal laser scanning microscope.

The invention discloses a novel fluorescent molecular probe, which has the general formula of the structure: (see the original text), wherein R1 refers to an alkyl chain provided with one to eight carbon atoms; and R2 refers to hydrogen, a sulfonic acid group, a sulfoacid ion radical or quaternary ammonium containing three to fifteen carbon atoms, which is connected at the 4, 5 or 6 position of an intermediate benzene ring. The invention further discloses application of the novel fluorescent molecular probe to the field of Hg2+ detection. The novel fluorescent molecular probe is high in selectivity and sensitivity on mercury ions. By means of combination of confocal laser scanning microscope technology, fluorescent imaging of the novel fluorescent molecular probe after the novel fluorescent molecular probe captures the mercury ions in a cell can be acquired.

The invention discloses an in-situ visualization method of dispersity of an inorganic filler in plastic, belongs to the technical field of filler dispersity in plastic and relates to a method for special identification dyeing screening of an inorganic filler in plastic after film formation. Based on special identification of boronic acid-modified fluorescent molecules and surface hydroxyl of a layered filler, the inorganic filler in a press-molded plastic film is subjected to fluorescent labeling and through a fluorescence microscope or a confocal laser scanning microscope, the visualization result is measured and analyzed. The method is free of pre-modification or treatment on the inorganic filler, only utilizes immersion of any plastic film in boric acid fluorescent molecules, is an in-situ, non-destructive, rapid and effective visual identification method and can be widely used for effective screening of an inorganic filler in a composite material in the factory.

The invention belongs to the field of medical apparatus, and particularly relates to a diagnosis and treatment integrated confocal hysteroscope system. A hard endoscope end part of a hard hysteroscope is provided with a treatment device; and a treatment system host, which is correspondingly used in conjunction with the treatment device, is connected to the hard hysteroscope. Particularly, the diagnosis and treatment integrated confocal hysteroscope system organically combines the hard hysteroscope, a confocal laser scanning microscope and a laser knife system or the hard hysteroscope, the confocal laser scanning microscope and a microwave knife system. Through the clinical application of the diagnosis and treatment integrated confocal hysteroscope system, the effect of performing diagnosis and treatment at the same time can be achieved, an optical system and a confocal laser scanning microscopic system can be used for observing the macroscopic condition and microscopic structures of a uterine cavity wall and pathologic changes in a uterine cavity respectively, and the laser knife system or the microwave knife system can perform laser treatment or microwave treatment on the pathologic changes under the direct vision of a monitor. By suing the diagnosis and treatment integrated confocal hysteroscope system to perform a hysteroscope operation, the problems in the diagnosis and the treatment of a patient can be solved, the frequent replacement of the endoscope can be avoided, a great deal of operation time can be saved, the paint of the patient can be alleviated, and the accuracy and the safety of the operation can be further improved.

A reference device for evaluating the performance of a confocal laser scan microscope. The reference device comprises a substrate (53) and reference fluorescing matter distributed over a surface of the substrate (53). The reference fluorescing matter has a predetermined spatial distribution over the latter surface.

Methods and apparatuses are disclosed whereby structured illumination microscopy (SIM) is applied to a scanning microscope, such as a confocal laser scanning microscope or sample scanning microscope, in order to improve spatial resolution. Particular aspects of the disclosure relate to the discovery of important advances in the ability to (i) increase light throughput to the sample, thereby increasing the signal / noise ratio and / or decreasing exposure time, as well as (ii) decrease the number of raw images to be processed, thereby decreasing image acquisition time. Both effects give rise to significant improvements in overall performance, to the benefit of users of scanning microscopy.

The invention provides a method for rapidly and efficiently determining the regeneration rate of plant cells after ultralow-temperature preservation, and relates to the technical field of cell survival rate detection, and the method can rapidly judge the advantages and disadvantages of an ultralow-temperature preservation scheme and determine the optimal treatment time and intensity; according tothe invention, fluorescence detection can be carried out through the laser confocal scanning microscope, so that the function of the laser confocal scanning microscope is effectively expanded, theoretical support is provided for designing a plant ultralow-temperature preservation scheme, and the method has important significance for developing a new generation of ultralow-temperature preservationtechnology. In the embodiment of the invention, the ratio of the dead cells to the living cells detected by the laser confocal scanning microscope is consistent with the ultralow-temperature regeneration rate of the embryonic cells, so that the ratio of the dead cells to the living cells can be detected by using the laser confocal scanning microscope, and an optimal ultralow-temperature preservation scheme is determined in advance.

The present invention relates to a laser scanning microscope that can generate an arbitrary control signal without depending on the configuration of the hardware. A memory 64 has a bit width wider than a bit width of drive data based on a drive table for generating a drive signal X for driving a scanner, which is driven by a drive circuit 66X, to scan a designated scanning area at a designated speed, and stores the drive table, and a controller 61 sets a control signal for controlling a predetermined operation in a predetermined mechanism that has a strict time relationship with the scanning based on the drive data, in bits in the bit width of the memory 64X, excluding the bits used by the drive data, and these mechanisms perform the predetermined operation based on the control signal that is read simultaneously when the drive data is read from the memory 64X. The present invention can be applied to a confocal laser scanning microscope that observes a sample by performing scanning with laser light.

The method of using the confocal laser scanning microscope system to measure the surface roughness of the medium involves the field of precision processing and precise measurement. The method includes designing the confocal laser scanning microscope system and setting an adjustable pinhole diaphragm in front of the detector; designing the The scanning range is 100 microns, the laser scanner with a control accuracy of 10 nanometers and the stage with adjustable object distance and inclination angle are selected. The laser with a wavelength of 405 nanometers is selected; the aperture pinhole is set to a value of <1.0 Airy unit, After selecting the scanning range and scanning layer thickness, scan and store the data; reconstruct the scanning image, select a shape curve in the plane, and give the average value of the peak roughness and total roughness of the shape curve; obtain multiple The average value of the peak roughness and the total roughness of the sampling length; the average value of the peak roughness and the total roughness is obtained by measuring the surface roughness of multiple waveguides with multiple sampling lengths; this method can be used in the production of large-scale micro-nano structures Non-destructive online fast detection in .

The invention relates to a method for evaluating the interfiber bonded area of high-yield pulp with CLSM as a main tool. The method comprises the following main steps: with commercial high-yield pulp as a raw material, subjecting fibrous material of the pulp to soaking, latency elimination, screening and other early-stage pretreatment; then carrying out fluorescent dyeing on fibers and building a fiber bonding model by using related tools; scanning the fiber bonding model by using the optical sectioning technology of CLSM and accurately positioning the interfiber bonded area by regulating CLSM scanning parameters; and acquiring the focal plane image of the interfiber bonded area and calculating the actual area of the bonded area so as to evaluate the interfiber bonded area of the high-yield pulp. The method provided by the invention does not need the procedures of preparation of handsheets from the high-yield pulp and ultrathin slicing of the handsheets, is simple to operate, easy to implement and applicable to evaluation of the interfiber bonded area of all types of high-yield pulp, and has good adaptability.

The invention relates to the technical field of manufacturing of 3D printing materials, in particular to a method for manufacturing an edible 3D printing material by using chickpea protein, and the method comprises the following steps: 1) purifying the chickpea protein; (2) preparing an oil-in-water emulsion; 3) measuring the size of the liquid drop; 4) carrying out rheological property measurement; 5) carrying out confocal laser scanning microscope (CLSM) measurement; and 6) carrying out 3D printing of the emulsion. The purpose of preparing the 3D printing emulsion by using the chickpea protein is achieved, the 3D printing emulsion has good stability and viscoelasticity and can be extruded to pass through a nozzle of a printer, adhered chickpea protein particles after pH adjustment can be used as physical crosslinking among oil drops, no additional treatment is needed, a stent with the elastic-plastic rheological property better than the critical stress, and the independence required by 3D printing is met.

Methods and apparatuses are disclosed whereby structured illumination microscopy (SIM) is applied to a scanning microscope, such as a confocal laser scanning microscope or sample scanning microscope, in order to improve spatial resolution. Particular aspects of the disclosure relate to the discovery of important advances in the ability to (i) increase light throughput to the sample, thereby increasing the signal / noise ratio and / or decreasing exposure time, as well as (ii) decrease the number of raw images to be processed, thereby decreasing image acquisition time. Both effects give rise to significant improvements in overall performance, to the benefit of users of scanning microscopy.

The invention belongs to the field of medical machinery, and particularly relates to a diagnosis and treat integral confocal cystoscope system which comprises a hard cystoscope, wherein a treatment unit is arranged at the end part of a hard endoscope of the hard cystoscope, and a host machine of a treatment system is connected onto the hard cystoscope and correspondingly matched with the treatment device for use. The diagnosis and treat integral confocal cystoscope system is organically combined with a laser scalpel system or a microwave scalpel system through the hard cystoscope and a confocal laser scanning microscope system. The clinical application of the diagnosis and treat integral confocal cystoscope system can realize the effect of simultaneous diagnosis and treatment, and also can solve the problems of two aspects of patient diagnosis and treatment, the endoscope is prevented from being frequently changed, a large amount of operation time is saved, the pain of a patient is relieved, furthermore, the operation accuracy and safety are improved, and an unexpected effect is obtained.

The invention discloses a targeting CpG nucleic acid drug delivery system and a preparation method thereof, and belongs to the field of biological medicine. The CpG nucleic acid drug delivery system comprises a carrier and a CpG nucleic acid drug, a carrier is made into water-soluble ammonification yeast beta-1,3-D-glucan through a specific mode, the surface of the water-soluble ammonification yeast beta-1,3-D-glucan has high positive charges, and the water-soluble ammonification yeast beta-1,3-D-glucan can be tightly combined with CpG through electrostatic interaction. The carrier is low in toxicity and good in biocompatibility, and can be combined with a specific recognition receptor Dectin-1 to target macrophages; and flow cytometry and laser confocal scanning microscope analysis show that the transfection efficiency is high. The low-toxicity CpG nucleic acid drug delivery system with good biocompatibility can realize targeted delivery of CpG nucleic acid drugs and improve the cell uptake efficiency.