Electric real-time adjustment method of two-dimensional pinhole for confocal laser scanning microscope

Abstract

The invention relates to an electric real-time adjustment method and an electric real-time adjustment system of a two-dimensional pinhole for a confocal laser scanning microscope. The electric real-time adjustment system comprises an X linear stepping motor, an X driving nut, a Y linear stepping motor, a Y driving nut, an X guide rail, an X sliding block, a Y guide rail, a Y sliding block, an X optocoupler, an X separation blade, a Y optocoupler, a Y separation blade, a light splitting plate, an imaging lens, a CCD (Charge Coupled Device), an electric control unit and the pinhole, wherein the X driving nut, the X sliding block and the X separation blade are mounted in a fastening manner, and the X sliding block is in sliding connection with the X guide rail; the Y driving nut, the Y sliding block and the Y separation blade are mounted in the fastening manner, and the Y sliding block is in sliding connection with the Y guide rail; the X driving nut does a linear motion under the driving of the X linear stepping motor; the Y driving nut does a linear motion under the driving of the Y linear stepping motor; the Y guide rail is fastened on the X sliding block; the pinhole is fastened in the Y sliding block. The electric real-time adjustment method and the electric real-time adjustment system can realize real-time electric adjustment on the two-dimensional direction position of the pinhole, and the influence caused by light spot position changes of laser introduced by other optical components in the system is eliminated.

Description

【Technical field】 [0001] The invention relates to the technical field of optical detection of microscopic objective lenses, in particular to a method and system for electric real-time adjustment of a two-dimensional pinhole for a laser scanning confocal microscope. 【Background technique】 [0002] In the laser scanning confocal microscope optical system, since there are a large number of optical devices that need to be switched in real time and there are adjustment errors between optical devices, the position of the incident laser light on the pinhole surface may drift after switching between different optical devices. The laser scanning confocal microscope uses a two-dimensional pinhole electric real-time adjustment method to realize real-time detection and adjustment of the pinhole to ensure that the pinhole is accurately positioned on the pinhole surface of the incident laser corresponding to the different optical components calibrated after assembly and adjustment. Locati...

AI Technical Summary

Problems solved by technology

[0002] In the laser scanning confocal microscope optical system, since there are a large number of optical devices that need to be switched in real time and there are adjustment errors between the optical devices, the position of the incident laser light on the pinhole surface may drift after switching between different optical devices

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