Novel fluorescent molecular probe and application thereof

A fluorescent molecular probe, a new type of technology, used in fluorescence/phosphorescence, material excitation analysis, organic chemistry, etc., can solve the problems of complex synthesis steps, unsuitable for industrialization, and lack of price competitiveness, and achieve high selectivity and The effect of sensitivity

Inactive Publication Date: 2012-06-20
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, the fluorescent molecular probe method can effectively measure the mercury content in living cells, and can be used for laser confocal cell fluorescence imaging, such as the fluorescent molecular probe formed by the combination of rhodamine and macrocycle with the following structure, which can measure Mercury content in cells [J.Org.Chem.2008, 73, 8571 8574], but its synthesis steps are complicated, the cost is high, it is not suitable for industrialization, and there is no price competitiveness

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  • Novel fluorescent molecular probe and application thereof
  • Novel fluorescent molecular probe and application thereof
  • Novel fluorescent molecular probe and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In this embodiment, the fluorescent molecular probe has the following molecular structural formula:

[0027]

[0028] Hg in solution by fluorescence method 2+ For selective recognition and determination: the fluorescent molecular probe was used at a concentration of 20 μM and 3 μM Hg 2+ Dispersed in dimethyl sulfoxide-water (volume ratio 1:9), incubated with mouse fibroblasts, observed with a Nikon Eclipse TE2000-5 fluorescence microscope (254nm excitation) and collected as follows: figure 2 Fluorescent images shown.

Embodiment 2

[0030] This example uses the same fluorescent molecular probe as that of Example 1.

[0031] The fluorescent molecular probe was treated with a concentration of 20 μM and 1 μM Hg 2+ Dispersed in dimethyl sulfoxide-water (volume ratio 1:9), incubated with mouse fibroblasts, observed with a Nikon Eclipse TE2000-5 fluorescence microscope (254nm excitation) and collected as follows: image 3 Fluorescent images shown.

Embodiment 3

[0033] In this embodiment, the fluorescent molecular probe has the following molecular structural formula:

[0034]

[0035] The fluorescent molecular probe at a concentration of 20 μM and 3 μM Hg 2+ Dispersed in dimethyl sulfoxide-water (volume ratio 1:9), incubated with mouse fibroblasts, observed with a Nikon Eclipse TE2000-5 fluorescence microscope (254nm excitation) and collected as follows: Figure 4 Fluorescent images shown.

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Abstract

The invention discloses a novel fluorescent molecular probe, which has the general formula of the structure: (see the original text), wherein R1 refers to an alkyl chain provided with one to eight carbon atoms; and R2 refers to hydrogen, a sulfonic acid group, a sulfoacid ion radical or quaternary ammonium containing three to fifteen carbon atoms, which is connected at the 4, 5 or 6 position of an intermediate benzene ring. The invention further discloses application of the novel fluorescent molecular probe to the field of Hg2+ detection. The novel fluorescent molecular probe is high in selectivity and sensitivity on mercury ions. By means of combination of confocal laser scanning microscope technology, fluorescent imaging of the novel fluorescent molecular probe after the novel fluorescent molecular probe captures the mercury ions in a cell can be acquired.

Description

technical field [0001] The invention relates to the technical field of fluorescent molecular probes, in particular to a novel fluorescent molecular probe and its application. Background technique [0002] In the research of life science, metal ions occupy an important position. Real-time online detection of the concentration and distribution of metal ions in the living system, and the exploration of the role played by various metal ions will provide the necessary basis for biological and medical research. Mercury is one of the most toxic heavy metals, and it is of great significance to develop a method for visual spectroscopic analysis of mercury ions in living systems. [0003] People have designed and synthesized fluorescent molecules that can effectively express molecular recognition information through the fluorescent signal of the molecule. In a certain system, when a certain physical or chemical property in the substance or system changes, the fluorescent signal of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D213/38G01N21/64
Inventor 孟庆华
Owner SHANGHAI JIAO TONG UNIV
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