Targeting CpG nucleic acid drug delivery system and preparation method thereof

A technology for delivering systems and compounds, applied in the field of biomedicine, can solve problems such as low efficiency and low stability, and achieve the effects of simple preparation process, good biocompatibility, and high-efficiency biocompatibility
CN113304277APending Publication Date: 2021-08-27JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2021-08-27

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Abstract

The invention discloses a targeting CpG nucleic acid drug delivery system and a preparation method thereof, and belongs to the field of biological medicine. The CpG nucleic acid drug delivery system comprises a carrier and a CpG nucleic acid drug, a carrier is made into water-soluble ammonification yeast beta-1,3-D-glucan through a specific mode, the surface of the water-soluble ammonification yeast beta-1,3-D-glucan has high positive charges, and the water-soluble ammonification yeast beta-1,3-D-glucan can be tightly combined with CpG through electrostatic interaction. The carrier is low in toxicity and good in biocompatibility, and can be combined with a specific recognition receptor Dectin-1 to target macrophages; and flow cytometry and laser confocal scanning microscope analysis show that the transfection efficiency is high. The low-toxicity CpG nucleic acid drug delivery system with good biocompatibility can realize targeted delivery of CpG nucleic acid drugs and improve the cell uptake efficiency.
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Description

technical field

[0001] The invention relates to a targeted CpG nucleic acid drug delivery system and a preparation method thereof, belonging to the field of biomedicine. Background technique

[0002] CpG oligonucleotide (hereinafter referred to as CpG ODN) is a kind of DNA rich in unmethylated cytosine-guanine dinucleotide. Unmethylated CpG motifs widely exist in the genomic DNA of bacteria or viruses, and the frequency of occurrence is much higher than that of vertebrates, and 80% of the carbon atom at the 5th position of cytosine in vertebrates is methylated. This significant difference allows CpG ODN to be regarded as a "danger signal" by the mammalian immune system to initiate an immune response. CpG ODN can be recognized by Toll-like receptor 9 (hereinafter referred to as TLR9) of immune cells to promote the secretion of various cytokines and induce innate or adaptive immune responses. It can be used as an effective immunotherapeutic tool in anti-infection immunity, ca...

Claims

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