36results about How to "Improve qualitative accuracy" patented technology

The invention claims a method for detecting urotropin in cigarette mainstream smoke through high performance liquid chromatography-tandem mass spectrum. The method comprises the steps of gathering mainstream smoke total particulate matter of 20 cigarettes according to the rule of ISO4387:2000, then putting a cambridge filter with the mainstream smoke total particulate matter into a 250mL bread bottle, adding 40 mL of ethanol extract liquor containing triethylamine to oscillate for extracting the cambridge filter for 40 minutes, then filtering through a 0.45 micron filter membrane to detect by using GC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry). Instrument conditions are as follows: a HILIC (Hydrophilic Interaction Chromatography) is used; the detection temperature is 30 DEG C; the sample size is 2microliter; the flow velocity is 0.25mL per minute; a ratio of a mobile phase V (acetonitrile) to V (5mmol / L ammonium acetate solution) is 75:25; the mobile phase carries out isocratic elution. Mass spectrum conditions are as follows: an ionization mode is electros spray; a scanning mode is positive ion; the atomizing gas is 40 psi; the curtain gas is 10 psi; the auxiliary trim gas is 40 psi; the collision gas is 5 psi; the ion source voltage is 4000-5000V; the ion source temperature is 250-350 DEG C. The method is simple to operate, good in separation effect, accurate to determine the nature, short in sample analysis time and high in detection sensitivity.

The invention discloses a method for detecting ethyl urethane content of cigarette sidestream smoke via LC-MS / MS combination. A fishtail hood and a glass fiber filter disc are utilized to collect ethyl urethane of cigarette sidestream smoke; ethanol is applied to extraction of the glass fiber filter disc and cleaning of the fishtail hood; and an extraction liquid is diluted and then purified by a solid phase extraction small column, and finally eluted via dichloromethane, an eluant is detected by an efficient liquid chromatogram / tandom mass spectrometer, and the content of the ethyl carbamate of the cigarette side flow smoke. The method is simple and convenient to operate, has a good separation effect and accurate determination, and is short in sample analysis time and high in detection sensitivity.

The invention discloses an electronic patient record clinical data check method and system. The method comprises the following steps: positioning each identification item according to a predefined patient record template; calling corresponding identification engines according to data types corresponding to the identification items for performing identification; judging the data types of the identification results; finally saving the identification results and the original images of the identification items corresponding to the identification results into corresponding data tables in a data base. Due to the adoption of the method and the system, the follow-up targeted check and verification is convenient, the check workload is reduced, the time and the labor are saved, and the use cost is greatly reduced. Moreover, during the check, the check identification results and corresponding original images are displayed on the same screen after being linked, thereby further reducing both the workload and using cost.

The invention discloses a method and a device for simultaneously detecting three metabolic products of chlorpyrifos in urine. The method comprises the following steps: (A) target compounds selection: selecting 3,5,6-trichloro-2-pyridoxol, triethyl phosphate and diethyl thiophosphate; (B) sample enzymolysis; (C) sample extraction; (D) sample purification and concentration; (E) instrumental analysis. The device mainly comprises a main body and a chromatography-ion tandem mass spectrum detector, wherein the main body comprises a power supply unit, a thawing unit, a centrifugal device I, a water bath device, a turbine mixing device, a centrifugal device II, a purification device, a rotary evaporation device and a PLC (programmable logic controller). According to the method disclosed by the invention, the detection effectiveness and the detection reliability are greatly improved, and the detection time and the detection cost are saved.

The invention discloses a method for detecting advanced glycation end products (AGEs) in main stream smoke of a cigarette. The method specifically comprises analysis and determination of carboxy methyl lysine (CML) and carboxyethyl lysine (CEL). The method comprises the following steps: trapping total particulate matter of cigarette smoke by virtue of a Cambridge filter; carrying out ultrasonic extraction on the filter with a hydrochloric acid solution; and carrying out analysis by combining a cation exchange solid-phase extraction column purification method with liquid chromatogram-tandem mass spectrometry (LC-MS / MS). The method has the characteristics that (1) a detection method for AGEs in the main stream smoke of the cigarette is built for the first time; (2) a sample pretreatment method is built according to the characteristics of cigarette smoke sample matrix, derivative reaction is not required, and the pretreatment is simple and efficient; and (3) the method is high in sensitivity, good in qualitative accuracy and suitable for analysis of large batches of samples.

The invention provides a method for detecting organochlorine pesticides in the atmosphere by means of gas chromatography-ion trap tandem mass spectrometry , which comprises the following steps: collecting samples, extracting samples, purifying and concentrating samples, and detecting the samples by chromatography-ion trap mass spectrometry. The invention enjoys higher selectivity and sensitivity than the prior detection methods of the target compounds through optimization of gas chromatography and tandem mass spectrometry conditional parameters. The invention adopts the selection response monitoring techniques (SRM) of gas chromatography / ion trap tandem mass spectrometry (GC / ITMS) to remove the disadvantage that the selectivity and sensitivity of trace amount OCPs in complex background matrix of the prior detectors can not be achieved at the same time. The invention has the advantages of low detecting cost, and accurate and reliable positioning, thus presenting very obvious superiority over the traditional detection devices GC / ECD, GC / LRMS and GC / HRMS.

The invention provides a method for quantitatively detecting six kinds of nitrobenzene compounds in fine atmospheric particles PM2.5. The method comprises the following steps: acquiring a sample, extracting the sample, concentrating and purifying the sample and quantitatively detecting by adopting chromatography-tandem mass spectrometry. By optimizing parameters of secondary mass spectrometry conditions, the method is better in selectivity and higher in sensitivity on target compounds in comparison with an existing detection method reported in literature. According to the method, the disadvantage that the selectivity and the sensitivity of a conventional detector in the process of detecting the trace quantity of nitrobenzene compounds in a complex background matrix cannot be ensured at the same time is overcome by virtue of a gas chromatography / tandem mass spectrometry selective reaction monitoring technology, and the qualitative operation is accurate and reliable, so that the method has very remarkable advantages in comparison with conventional GC / ECDs (gas chromatography / election capture detectors), GC / NPDs (gas chromatography / nitrogen-phosphorus detector), GC / LRMS (gas chromatography / low resolution mass spectrometry) detectors or GC / HRMS (gas chromatography / high resolution mass spectrometry) detectors.

The invention provides a method for quantitatively detecting organophosphorus pesticide chlorpyrifos in environmental air, and belongs to the field of detection methods for pesticide residues. The method comprises collection of a sample (comprising a gas phase part and a particulate matter part), pretreatment of the sample and chromatography-tandem mass spectrometry quantitative detection. Compared with reported methods, the detection of the chlorpyrifos in the environmental air is higher in selectivity and higher in sensitivity through the optimization of parameters of a chromatography-mass spectrometry detection method. According to the method, by the use of a multi-reaction monitoring technology of high performance liquid chromatography / tandem mass spectrometry, the shortcoming that a conventional detector cannot have the selectivity and the sensitivity at the same time during detection of a trace chlorpyrifos compound in a complicated background matrix is overcome, and qualitation and quantitation are more accurate and reliable. Therefore, compared with conventional UV-Vis, HPLC / PDA, GC / ECD and GC / NPD, the method provided by the invention has very outstanding advantages.