40results about How to "Meet the requirements of quantitative analysis" patented technology

The invention discloses a method for synchronously detecting a plurality of organophosphorus fire retardants in bottom mud, belonging to the detection field of trace amount of organophosphorus fire retardants in the environment. The method comprises the main steps of firstly, extracting a target object in a sample through an accelerated solvent extraction instrument; purifying by a gel permeation chromatography; further purifying and enriching by adopting a solid-phase small extraction column; and finally, concentrating and making up to the constant volume and detecting and quantifying by using a gas chromatograph-mass spectrometer. According to the invention, a pre-treatment method for the organophosphorus fire retardants in the bottom mud is established and optimized, the automation degree is high and the repeatability is good; the gas chromatograph-mass spectrometer is used for carrying out quantitative detection, the detection limit is low and the sensitivity is high; the detection limit to nine types of the organophosphorus fire retardants are lower than 0.340 microgram per gram. According to the method, the synchronous analysis and detection on the plurality of trace amount of organophosphorus fire retardants in a complicated environment medium, namely the bottom mud, is realized; the sensitivity and the accuracy are achieved, and the method makes up the disadvantages of the technology in the field.

The invention provides a method for quantitatively detecting six kinds of nitrobenzene compounds in fine atmospheric particles PM2.5. The method comprises the following steps: acquiring a sample, extracting the sample, concentrating and purifying the sample and quantitatively detecting by adopting chromatography-tandem mass spectrometry. By optimizing parameters of secondary mass spectrometry conditions, the method is better in selectivity and higher in sensitivity on target compounds in comparison with an existing detection method reported in literature. According to the method, the disadvantage that the selectivity and the sensitivity of a conventional detector in the process of detecting the trace quantity of nitrobenzene compounds in a complex background matrix cannot be ensured at the same time is overcome by virtue of a gas chromatography/tandem mass spectrometry selective reaction monitoring technology, and the qualitative operation is accurate and reliable, so that the method has very remarkable advantages in comparison with conventional GC/ECDs (gas chromatography/election capture detectors), GC/NPDs (gas chromatography/nitrogen-phosphorus detector), GC/LRMS (gas chromatography/low resolution mass spectrometry) detectors or GC/HRMS (gas chromatography/high resolution mass spectrometry) detectors.

The invention relates to an ultra-high performance liquid chromatography-tandem mass spectrometry detection method. On the basis of an ultra-high performance liquid chromatography-tandem mass spectrometry detection platform, firstly, mass spectrometry detection parameters and chromatography separation conditions are optimized one by one by use of standard substances of target metabolites; an extraction method of 15 key target metabolites (including 4 lysolecithin, 3 unsaturated fatty acids and 8 bile acids) in blood plasma is further explored with an optimized chromatography-mass spectrometry method; LOD (limit of detection), LOQ (limit of quantitation), degree of precision, accuracy, the linear range and the like of the metabolites, detected simultaneously, in the blood plasma and urine are studied with an optimized analysis method. Results prove that the detection method can rapidly, accurately, specifically and sensitively quantify the content of the 15 key target metabolites in a body fluid, and a foundation is laid for screening of non-intrusive clinical diagnostic markers of liver and gall diseases.

The invention relates to a method for quickly detecting an extracting solution of Ganmaoling granules by utilizing near-infrared spectrometry. The method comprises the steps of linarin content determination and/or chlorogenic acid content determination and/or solid content determination. According to the method provided by the invention, linarin content, chlorogenic acid content and solid content near-infrared online analytical methods are established in an extraction process of the Ganmaoling granules, and are applied to online detection, so that automatic detection and real-time prediction of various index contents of the extraction process of the Ganmaoling granules are realized; an established model is high in prediction precision, and high in accuracy, and meets the requirement of quantitative analysis in actual production; by the method, quick and efficient real-time monitoring and control can be carried out on quality of the extraction process, so that the safety, stability and effectiveness of the final product quality are favorably ensured.

The invention discloses a method for synchronously extracting and purifying various chlorinated polycyclic aromatic hydrocarbons in soil, which belongs to the field of detection of the chlorinated polycyclic aromatic hydrocarbons. The method comprises the following steps: firstly removing moisture in a soil sample by virtue of a freezing dryer, grinding the soil sample, and filtering the ground soil sample by virtue of a 200-mesh sieve for later use; and extracting a target matter in the soil sample by utilizing an acceleration solvent extractor, purifying the target matter in a gel permeation chromatography, finally concentrating the target matter and sizing the target matter. According to the method, a preprocessing method of the chlorinated polycyclic aromatic hydrocarbons in the soil is established, less solvent is used in the entire process, the automation degree is high, less time is used, the safety is high, fewer steps are used, and various chlorinated polycyclic aromatic hydrocarbons in the soil can be accurately, qualitatively and quantitatively determined. The chlorinated polycyclic aromatic hydrocarbons in the soil are quantitatively detected by utilizing the gas phase chromatography-mass spectrometry, the detection limit is low (when S/N is equal to 3, the detection limit is 2.6 to 25pg/g), the precision is high (RSD=6.30 to 12.0 percent), the blank mark recovery rate is 64.1 to 109.2 percent, the synchronous analysis and detection of various trace chlorinated polycyclic aromatic hydrocarbons in the complicated environment medium such as the soil can be realized, sensitivity and accuracy can be realized, and the weaknesses of the prior art can be overcome.

The invention provides a method for quantitatively detecting organophosphorus pesticide chlorpyrifos in environmental air, and belongs to the field of detection methods for pesticide residues. The method comprises collection of a sample (comprising a gas phase part and a particulate matter part), pretreatment of the sample and chromatography-tandem mass spectrometry quantitative detection. Compared with reported methods, the detection of the chlorpyrifos in the environmental air is higher in selectivity and higher in sensitivity through the optimization of parameters of a chromatography-mass spectrometry detection method. According to the method, by the use of a multi-reaction monitoring technology of high performance liquid chromatography/tandem mass spectrometry, the shortcoming that a conventional detector cannot have the selectivity and the sensitivity at the same time during detection of a trace chlorpyrifos compound in a complicated background matrix is overcome, and qualitation and quantitation are more accurate and reliable. Therefore, compared with conventional UV-Vis, HPLC/PDA, GC/ECD and GC/NPD, the method provided by the invention has very outstanding advantages.

The invention relates to a tobacco shred blending uniformity determination method based on combination of a chromatic value and an entropy weight method. Chromaticity values L *, a * and b * of the formula tobacco shreds taken at different time points are measured by adopting a color difference meter, variable coefficients of three chromaticity values of an evaluation sample are calculated, then each chromaticity value is weighted by using an entropy weight method to obtain a comprehensive variable coefficient of the three chromaticity values of the evaluation sample, finally a method for calculating the tobacco shred blending uniformity is established according to the comprehensive variable coefficient of the chromaticity values, and the test result of the method is compared with an industrial standard method. The result shows that the variable coefficient of the precision test for determining the tobacco shred blending uniformity by combining the chromaticity value with the entropy weight method and the variable coefficient of the repeatability test meet the requirements of quantitative analysis. Compared with an industrial standard method, the method has the advantages that thedetermination result of the tobacco shred blending uniformity has no significant difference, and the variable coefficient of the test result is smaller. The method has the advantages of simple operation, no chemical addition, high precision and good repeatability, and is suitable for accurate determination of the tobacco shred blending uniformity.

The invention discloses a method for measuring the residual amount of three types of dithiocarbamate in tobacco. To-be-tested tobacco powder is processed by an alkali extracting solution and then processed powder is filtered; the filter solution is collected and the analysis is carried out by LC-MS-MS; and then standard curve drawing and calculation are carried out to obtain a result. According tothe invention, the residual amount of three types of DCs materials in tobacco is measured accurately for the first time. The linear range of the three DCs materials is from 20mg/L to 100mg/L; the correlation coefficients of the three DCs materials are not less than 0.99 respectively; the detection limits are 2.3 microgram/kg, 4.3 microgram/kg, and 5.2microgram/kg respectively; the quantitation limits are 7.7 microgram/kg, 14.3 microgram/kg, and 17.3microgram/kg; the matrix effect ranges are from 0.19 to 0.50, from 0.78 to 1.70, and from 0.35 to 0.87; the intra-day RSDs of all DTCs of tobaccomatrixes are from 11.33% to 16.80% and the inter-day RSDs are from 5.84 to 6.54%; and the adding standard recovery is from 84.5% to 103.5%. According to the method disclosed by the invention, the operation becomes simple and convenient; the analysis speed is fast; the accuracy and precision as well as the stability are high; and the quantitative analysis requirement is met.

The invention relates to a method for rapidly detecting a preparation process of Qizhi Weitong granules with NIRS (near infrared spectroscopy) and an application. An NIRS online analysis method for a water extraction process, a volatile oil extraction process and an extracting solution concentration process of the preparation process of the Qizhi Weitong granules is established and applied to online detection, so that automatic detection of the preparation process of the Qizhi Weitong granules and real-time prediction of content of each quality control index are realized; an established model is high in prediction precision and accuracy and meets the requirement for quantitative analysis in actual production; compared with a traditional pharmacopeia method, the method has the advantages that on the premise that the accuracy is guaranteed, the analysis efficiency is increased greatly, the quality of the extraction processes can be monitored and controlled in real time rapidly and efficiently, and accordingly, guarantee of safety, stability and effectiveness of the quality of final products is facilitated.

The invention discloses a method for measuring tomatidine and tomato saponin by high performance liquid chromatography-mass spectrometry. The method comprises the following steps of: (1) preprocessinga sample to be tested; (2) measuring the content of tomatine and tomato saponin in the preprocessed sample by liquid chromatography-mass spectrometry. The method for measuring tomatidine and tomato saponin by high performance liquid chromatography-mass spectrometry realizes simultaneous detection of tomatidine and tomato saponin in tomato sample materials. The method meets the requirement of quantitative analysis in precision, accuracy, and stability. The method has the characteristics of high accuracy, good reproducibility, stability, and reliability. The method can also be used for quantitative analysis of changes in tomatine content during tomato breeding.

The invention discloses a method for simultaneously detecting the content of water-soluble anions and cations in atmospheric particulate matters through a rapid solvent extraction-ion chromatography method, and relates to the field of atmospheric monitoring and detection.The technical scheme is characterized in that a rapid solvent extraction-ion chromatography method is used, and the method comprises the following steps: testing by a standard solution to obtain anion and cation standard spectrograms (Figure 1 and Figure 2) and a standard curve of a target compound, then pretreating a sample, testing on a machine, finding out a corresponding target peak by combining with the standard spectrograms, and calculating the concentration of the target compound according to the standard curve. According to the whole operation mode, the peak pattern of the target compound is sharp, the responsivity is high, the relative standard deviation is small, and the precision is relatively good, so that the test accuracy is effectively improved.

The invention discloses a gas chromatography method for detecting C18 fatty acid isomers in grease. A high-polarity ionic liquid gas chromatographic column with the specifications of 200 m*0.25 mm*0.20 mu m is used for detecting the C18 fatty acid isomers in the grease. By means of the method, the separation degree of C18:1 and C18:2 isomers is improved and C18:3 isomers are effectively separated. According to the established C18 fatty acid isomer method, the RSD (relative standard deviation) of the within-day precision is controlled in the range of 0.83%-4.30%, the RSD of the day to day precision is controlled in the range of 1.53%-3.90%, and the quantitative analysis requirements are met. According to the established C18 fatty acid isomer method, the detection limit ranges from 0.009 mg/L to 0.075 mg/L, and the minimum determination limit ranges from 0.028 mg/L to 0.225 mg/L, and the detection limit and the determination limit of C18 cis-trans-isomers are remarkably reduced.

The invention belongs to the field of traditional Chinese medicine, and particularly relates to a method for simultaneously measuring the contents of six chemical components in herba pogostemonis. Inorder to improve the quality control level of medicinal materials of herba pogostemonis and establish more scientific and reasonable quality standards, a UPLC is adopted for measuring the contents ofthe six components in the medicinal materials of herba pogostemonis for the first time, and a certain experimental basis is provided for improving quality control methods for the medicinal materials of herba pogostemonis and related preparations of herba pogostemonis.

The invention relates to a method for rapidly detecting a concentration process of an extracting solution during preparation of Qizhiweitong granules with NIRS (near infrared spectroscopy) and an application. An NIRS online analysis method for the concentration process of the extracting solution during preparation of the Qizhiweitong granules is established and applied to online detection, and automatic detection of the concentration process of the extracting solution during preparation of the Qizhiweitong granules and real-time prediction of content of various quality control indexes are realized; an established model is high in prediction precision and accuracy and meets the requirement of quantitative analysis in actual production; compared with the traditional pharmacopoeia method, the method has the advantages that the analysis efficiency is increased greatly on the basis of accuracy, and the quality of the concentration process of the extracting solution can be monitored and controlled in real time rapidly and efficiently, so that the safety, the stability and the effectiveness of the final product quality are guaranteed.

The invention relates to the field of drug detection, in particular to an establishment method for a near-infrared quantitative correction model of Jingyaokang Capsules and a detection method for Jingyaokang Capsules. The invention utilizes AOTF-NIR technology to establish NIR models of strychnine, hydroxysafflor yellow A, and moisture index of Jingyaokang Capsule finished products, which can simultaneously and quickly predict contents of multiple indexes in the finished products without cumbersome sample processing, and prediction results are accurate. The methods not only shorten a detectionperiod, reduce the use of consumables, and reduce detection costs, but also enable on-site analysis. And therefore, a fast and efficient detection method for the quality control of Jinguankang Capsules is provided. Compared with the traditional pharmacopoeia method, the methods greatly improve the analysis efficiency on the basis of ensuring the accuracy, and can quickly and efficiently control the product quality, thereby ensuring the safety, stability and effectiveness of the product quality.

The invention belongs to the field of analysis and testing, and relates to the development of a miniaturized low-temperature chromatographic column box. The feature of this device is that the refrigeration module is made of heat-conducting adhesive solidified at room temperature, and the capillary chromatographic column used for separation is also solidified in the middle of the refrigeration module. Using a 4cm x 4cm x 0.2cm semiconductor element for refrigeration does not require traditional liquid nitrogen or liquid carbon dioxide refrigerants. The entire column box has a volume of only 6cm×6cm×2.5cm, which is simple and convenient, and has low manufacturing cost.

The invention discloses a method for rapidly detecting triazole bactericides in soil. The detection method comprises the following steps: 1) establishing a standard curve of the triazole bactericides,2) adopting an improved QuEChERS method to carry out pretreatment on a soil sample, and 3) carrying out qualitative and quantitative determination on the triazole bactericides in combination with thestandard curve.